LOCUS       Exported               11152 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Overexpression of Progerin with a GFP fusion protein, under human 
            synapsin promotor.
ACCESSION   .
VERSION     .
KEYWORDS    pLenti-hSyn-PROGERIN
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11152)
  AUTHORS   Miller JD, Ganat YM, Kishinevsky S, Bowman RL, Liu B, Tu EY, Mandal 
            PK, Vera E, Shim JW, Kriks S, Taldone T, Fusaki N, Tomishima MJ, 
            Krainc D, Milner TA, Rossi DJ, Studer L
  TITLE     Human iPSC-based modeling of late-onset disease via progerin-induced
            aging.
  JOURNAL   Cell Stem Cell. 2013 Dec 5;13(6):691-705. doi: 
            10.1016/j.stem.2013.11.006.
  PUBMED    24315443
REFERENCE   2  (bases 1 to 11152)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11152
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        77..181
                     /gene="bla"
                     /label=AmpR promoter
     CDS             182..1042
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(400..419)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1213..1801
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1702..1721
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1955..1972
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2089..2110
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2125..2155
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2163..2179
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2168..2190
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2187..2203
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2187..2203
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    2252..2374
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2263..2282)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2318..2337
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(2504..2878)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(2897..2944)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(2992..3321)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3006..3141
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3060..3079)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     3306..3326
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(3335..3763)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3445..3466)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3657..3676
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    3809..4033
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     4022..4039
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             4065..4245
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     4750..4766
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     4751..4767
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    4770..5358
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             4876..4887
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5285..5305
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7333..8049)
                     /codon_start=1
                     /product="Aequorea coerulescens GFP"
                     /label=AcGFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK"
     promoter        complement(8063..8511)
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     misc_feature    8586..8703
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(9001..9045)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9230..9463
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    9956..10081
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             10128..10308
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(10326..10524)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(10351..10371)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        10526..10905
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     11077..11096
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tactgagagt gcaccatagg ggatcgggag atctcccgat ccgtcgacgt caggtggcac
       61 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
      121 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
      181 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
      241 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
      301 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
      361 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
      421 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
      481 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
      541 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
      601 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
      661 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
      721 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
      781 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
      841 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
      901 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
      961 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     1021 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     1081 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     1141 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     1201 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     1261 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     1321 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     1381 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     1441 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     1501 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     1561 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     1621 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     1681 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     1741 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     1801 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     1861 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     1921 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     1981 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     2041 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     2101 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     2161 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct
     2221 ctagctagag gtcgacggta tacagacatg ataagataca ttgatgagtt tggacaaacc
     2281 acaactagaa tgcagtgaaa aaaatgcttt tatttgtgaa atttgtgatg ctattgcttt
     2341 atttgtaacc attataagct gcaataaaca agttggggtg ggcgaagaac tccagcatga
     2401 gatccccgcg ctggaggatc atccagccgg cgtcccggaa aacgattccg aagcccaacc
     2461 tttcatagaa ggcggcggtg gaatcgaaat ctcgtagcac gtgtcagtcc tgctcctcgg
     2521 ccacgaagtg cacgcagttg ccggccgggt cgcgcagggc gaactcccgc ccccacggct
     2581 gctcgccgat ctcggtcatg gccggcccgg aggcgtcccg gaagttcgtg gacacgacct
     2641 ccgaccactc ggcgtacagc tcgtccaggc cgcgcaccca cacccaggcc agggtgttgt
     2701 ccggcaccac ctggtcctgg accgcgctga tgaacagggt cacgtcgtcc cggaccacac
     2761 cggcgaagtc gtcctccacg aagtcccggg agaacccgag ccggtcggtc cagaactcga
     2821 ccgctccggc gacgtcgcgc gcggtgagca ccggaacggc actggtcaac ttggccatgg
     2881 tttagttcct caccttgtcg tattatacta tgccgatata ctatgccgat gattaattgt
     2941 caacacgtgc tgatcagatc cgaaaatgga tatacaagct cccgggagct ttttgcaaaa
     3001 gcctaggcct ccaaaaaagc ctcctcacta cttctggaat agctcagagg cagaggcggc
     3061 ctcggcctct gcataaataa aaaaaattag tcagccatgg ggcggagaat gggcggaact
     3121 gggcggagtt aggggcggga tgggcggagt taggggcggg actatggttg ctgactaatt
     3181 gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc cacacctggt
     3241 tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt
     3301 tccacaccct aactgacaca cattccacag aattaattcg cgttaaattt ttgttaaatc
     3361 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
     3421 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
     3481 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
     3541 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
     3601 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
     3661 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
     3721 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtggggata ccccctagag
     3781 ccccagctgg ttctttccgc ctcagaagcc atagagccca ccgcatcccc agcatgcctg
     3841 ctattgtctt cccaatcctc ccccttgctg tcctgcccca ccccaccccc cagaatagaa
     3901 tgacacctac tcagacaatg cgatgcaatt tcctcatttt attaggaaag gacagtggga
     3961 gtggcacctt ccagggtcaa ggaaggcacg ggggaggggc aaacaacaga tggctggcaa
     4021 ctagaaggca cagtcgaggc tgatcagcgg gtttaaacgg gccctgctag agattttcca
     4081 cactgactaa aagggtctga gggatctcta gttaccagag tcacacaaca gacgggcaca
     4141 cactacttga agcactcaag gcaagcttta ttgaggctta agcagtgggt tccctagtta
     4201 gccagagagc tcccaggctc agatctggtc taaccagaga gacccagtac agtccggatg
     4261 cagctctcgg gccatgtgat gaaatgctag gcggctgtca aacctccact ctaatacttc
     4321 tctctccggg tcatccatcc catgcaggct cacagggtgt aacaagcggg tgttctctcc
     4381 ttcattggct tcttctacct tctcttgctc aactggtact agcttgtagc accatccaaa
     4441 ggtcagtgga tatctgatcc ctggccctgg tgtgtagttc tgccaatcag ggaagtagcc
     4501 ttgtgtgtgg tagatccaca gatcaaggat atcttgtctt cgttgggagt gaattagccc
     4561 ttccagtccc cccttttctt ttaaaaagtg gctaagatct acagctgcct tgtaagtcat
     4621 tggtcttaaa ggtacctgag gtgtgactgg aaaacccacc tcctcctcct cttgtgcttc
     4681 tagccaggca caatcagcat tggtagctgc tgtattgcta cttgtgattg ctccatgttt
     4741 ttctaggtct cgaggtcgac ggtatcgatg cggggaggcg gcccaaaggg agatccgact
     4801 cgtctgaggg cgaaggcgaa gacgcggaag aggccgcaga gccggcagca ggccgcggga
     4861 aggaaggtcc gctggattga gggccgaagg gacgtagcag aaggacgtcc cgcgcagaat
     4921 ccaggtggca acacaggcga gcagccaagg aaaggacgat gatttccccg acaacaccac
     4981 ggaattgtca gtgcccaaca gccgagcccc tgtccagcag cgggcaaggc aggcggcgat
     5041 gagttccgcc gtggcaatag ggagggggaa agcgaaagtc ccggaaagga gctgacaggt
     5101 ggtggcaatg ccccaaccag tgggggttgc gtcagcaaac acagtgcaca ccacgccacg
     5161 ttgcctgaca acgggccaca actcctcata aagagacagc aaccaggatt tatacaagga
     5221 ggagaaaatg aaagccatac gggaagcaat agcatgatac aaaggcatta aagcagcgta
     5281 tccacatagc gtaaaaggag caacatagtt aagaatacca gtcaatcttt cacaaatttt
     5341 gtaatccaga ggttgattat cgataagctt gatatcgaat tcttacatga tgctgcagtt
     5401 ctgggggctc tgggctcctg agccgctggc agatgccttg tcggcaggct gcccgcaggt
     5461 cccgcacagc acggtgcgcg agcgcaggtt gtactcagcg gggtcccccg agctgctgca
     5521 gtgggagcca tggtggtgat ggagcaggtc atctccatcc tcatcctcgt cgtcctcaac
     5581 cacagtcact gagcgcacca gcttgcgcat ggccacttct tccccagtgg agttgatgag
     5641 agccgtacgc aggctgttcc cgcagcccca ggtgttctgt gccttccaca ccaggtcggt
     5701 aggggggctg tgggtggccc cagctcctgc agcccagatc gtcaccacct gcccagcctt
     5761 cagggtgaac tttggtggga accggtaagt cagcaaggga tcatctccat tctggcgctt
     5821 gatctgccaa ttgcccatgg actggtcctc attggacttg ttgcgcagcc ggacaaactt
     5881 gccctcctca tccacctcct ccacggccac gcgcccgcta gtgcgtgcgt gctgtgagaa
     5941 gctgctgcgg ctctcagtgg actccagttt gcgctttttg gtgacgctgc ccccaccctg
     6001 tgtctgggat gagtgagagg aagcacggcc acggctgcgc tgcgaggtag ggctggggga
     6061 caggcgtagc ctctcctcct cgccctccaa gagcttgcgg taggcgtgga tctccatgtc
     6121 cagggccagc ttgatgtcca gaagctcctg gtactcgtcc agctgctgct gcatccttgc
     6181 ccgcatctcg gccatctccc gctccttttc cgccagcagc cgccggctgg tgtcccgctc
     6241 acgggccagt gagtcctcca ggtctcgaag cttcgcctcc ttggctgcca gctgcttctg
     6301 gagctggctg agctgggcag agaggctgtc gatgcggatg cgcgactgct gcagctcctc
     6361 gtgggcagcc cccaccaggt tgctgttcct ctcagcagac tgcctggcat tgtccagctt
     6421 ggcagaataa gtcttctcca gctccttctt atactgctcc acctggtcct catgctgggc
     6481 ccgcagttcc tgcagcgcat ccgccagccg gctctcaaac tcacgctgct tcccattgtc
     6541 aatctccacc agtcgggtct catgacggcg cttggtctca cgcagctcct cactgtagat
     6601 gttcttctgg aagtccagtt cctccttcat ggtctgcagc ctgttctcag catccacccg
     6661 ccgcagcatc tcatcctgaa gttgcttctt ggcctcacct agggctgcct caagcttggc
     6721 cacctggccc cgcagatcat gcagctcgcc ctccagcgtg cgcttctcac tgagagcagt
     6781 gctcagtgcg gcctccttgg agttcagcag agcctccagg tccttcagcc gagcctgagc
     6841 agctatcagg tcaccctcct tcttggtatt gcgcgctttc agctccttaa actcctcacg
     6901 cactttgctc agctccagct gcaggcgggc gcgctccttg gctactgagt caagggtctt
     6961 gcgggcatcc ccgagctcgg cctcgtaggc ggccttgatg ccggacacct cgcggctgac
     7021 cacctcttca gactcggtga tgcgaaggcg cagccctgcg ttctccgttt ccagcgagcg
     7081 cacacggtcg atgtagaccg ccaagcgatc attgagctcc tgcaggtcct ccttctcctg
     7141 cagccgggtg atgcgggtgg gcgacagcgg agtggagctg gcctgcgccc cgctgcgggt
     7201 ggcgcgccgc tgggacgggg tctccatggc cggcaggttg gcagcgctgc ccgcggggca
     7261 ggggtcccgg aaagggcgcg gggctcgggt cgaaggacag agactgctgt cgacagaggc
     7321 cttaagtccg gacttgtaca gctcatccat gccgtgggtg atggcggcgg cggtcacgaa
     7381 gccgaagtag atcatgtgat cgcgcttctc gttggggtcc ttggacaggg cgctctgggt
     7441 ggacaggtag tggttatcgg gcagcagcac agggccatcg ccgatggggg tattctgctg
     7501 gtagtggtcg gccagctgca cgctgccatc ctcgatgttg tggcggatct tgaagttcac
     7561 cttgatgcca ttcttggcct tgtcggtcat gatgtacaca ttgtgggcgt tgtagttgta
     7621 ctccatctta ttgcccagga tgttgccatc ctccttgaaa tcggtgccgg tcagctcgat
     7681 gcgattcacc agggtatcgc cctcgaactt cacctcggcg cgcgacttgt agttgccgtc
     7741 atcctcgaag aagatggtgc gctcctggat gtagccctca ggcatggcgc tcttgaagaa
     7801 gtcgtgctgc ttcatgtgat cggggtagcg tgagaagcac tgcacgccgt agctcagggt
     7861 ggtcaccagg gtgggccagg gcacaggcag cttgccggtg gtgcagatga acttcagggt
     7921 cagcttgccg taggtggcat cgccctcgcc ctcgccgctc acgctgaact tgtggccatt
     7981 cacatcgcca ttcagctcga tcaggatggg cacgatgccg gtgaacagct cggcgccctt
     8041 gctcaccata ccggtttctc gactgcgctc tcaggcacga cacgactcct ccgctgccca
     8101 ccgcagactg aggcagcgct gagtcgccgg cgccgcagcg cagatggtcg cgcccgtgcc
     8161 cccctatctc gcgcctcgcg tggtgcggtc cggctgggcc ggcggcggcg cggacgcgac
     8221 caaggtggcc gggaagggga gtttgcgggg gaccggcgag tgacgtcagc gcgccttcag
     8281 tgctgaggcg gcggtggcgc gcgccgccag gcgggggcga aggcactgtc cgcggtgctg
     8341 aagctggcag tgcgcacgcg cctcgccgca tcctgtttcc cctccccctc tctgataggg
     8401 gatgcgcaat ttggggaatg gggggttggg tgcttgtcca gtgggtcggg gtcggtcgtc
     8461 atgtaggcac ccccaccccg cctcatcctg gtcctaaaac ccacttgcac tcatacgcag
     8521 ggccctctgc agtctagaca cttaattaac caaactggat ctctgctgtc cctgtaataa
     8581 acccgaaaat tttgaatttt tgtaatttgt ttttgtaatt ctttagtttg tatgtctgtt
     8641 gctattatgt ctactattct ttcccctgca ctgtaccccc caatcccccc ttttctttta
     8701 aaattgtgga tgaatactgc catttgtctg cagaattggc gcacgcagtg ccgatccgtt
     8761 cactaatcga atggatctgt ctctgtctct ctctccacct tcttcttcta ttccttcggg
     8821 cctgtcgggt cccctcgggg ttgggaggtg ggtctgaaac gataatggtg aatatccctg
     8881 cctaactcta ttcactatag aaagtacagc aaaaactatt cttaaaccta ccaagcctcc
     8941 tactatcatt atgaataatt ttatatacca cagccaattt gttatgttaa accaattcca
     9001 caaacttgcc catttatcta attccaataa ttcttgttca ttcttttctt gctggttttg
     9061 cgattcttca attaaggagt gtattaagct tgtgtaattg ttaatttctc tgtcccactc
     9121 catccaggtc gtgtgattcc aaatctgttc cagagattta ttactccaac tagcattcca
     9181 aggcacagca gtggtgcaaa tgagttttcc agagcaaccc caaatcccca ggagctgttg
     9241 atcctttagg tatctttcca cagccaggat tcttgcctgg agctgcttga tgccccagac
     9301 tgtgagttgc aacagatgct gttgcgcctc aatagccctc agcaaattgt tctgctgctg
     9361 cactatacca gacaataatt gtctggcctg taccgtcagc gtcattgacg ctgcgcccat
     9421 agtgcttcct gctgctccca agaacccaag gaacaaagct cctattccca ctgctctttt
     9481 ttctctctgc accactcttc tctttgcctt ggtgggtgct actcctaatg gttcaatttt
     9541 tactacttta tatttatata attcacttct ccaattgtcc ctcatatctc ctcctccagg
     9601 tctgaagatc agcggccgct tgctgtgcgg tggtcttact tttgttttgc tcttcctcta
     9661 tcttgtctaa agcttccttg gtgtctttta tctctatcct ttgatgcaca caatagaggg
     9721 ttgctactgt attatataat gatctaagtt cttctgatcc tgtctgaagg gatggttgta
     9781 gctgtcccag tatttgtcta cagccttctg atgtttctaa caggccagga ttaactgcga
     9841 atcgttctag ctccctgctt gcccatacta tatgttttaa tttatatttt ttctttcccc
     9901 ctggccttaa ccgaattttt tcccatcgcg atctaattct cccccgctta atactgacgc
     9961 tctcgcaccc atctctctcc ttctagcctc cgctagtcaa aatttttggc gtactcacca
    10021 gtcgccgccc ctcgcctctt gccgtgcgcg cttcagcaag ccgagtcctg cgtcgagaga
    10081 gctcctctgg tttccctttc gctttcaagt ccctgttcgg gcgccactgc tagagatttt
    10141 ccacactgac taaaagggtc tgagggatct ctagttacca gagtcacaca acagacgggc
    10201 acacactact tgaagcactc aaggcaagct ttattgaggc ttaagcagtg ggttccctag
    10261 ttagccagag agctcccagg ctcagatctg gtctaaccag agagacccag tacaggcaaa
    10321 acgcgctgct tatatagacc tcccaccgta cacgcctacc gcccatttgc gtcaatgggg
    10381 cggagttgtt acgacatttt ggaaagtccc gttgattttg gtgccaaaac aaactcccat
    10441 tgacgtcaat ggggtggaga cttggaaatc cccgtgagtc aaaccgctat ccacgcccat
    10501 tgatgtactg ccaaaaccgc atcaccatgg taatagcgat gactaatacg tagatgtact
    10561 gccaagtagg aaagtcccat aaggtcatgt actgggcata atgccaggcg ggccatttac
    10621 cgtcattgac gtcaataggg ggcgtacttg gcatatgata cacttgatgt actgccaagt
    10681 gggcagttta ccgtaaatac tccacccatt gacgtcaatg gaaagtccct attggcgtta
    10741 ctatgggaac atacgtcatt attgacgtca atgggcgggg gtcgttgggc ggtcagccag
    10801 gcgggccatt taccgtaagt tatgtaacgc ggaactccat atatgggcta tgaactaatg
    10861 accccgtaat tgattactat taataactag tcaataatca atgtcaacgc gtatatctgg
    10921 cccgtacatc gcgaagcagc gcaaaacgcc taaccctaag cagattcttc atgcaattgt
    10981 cggtcaagcc ttgccttgtt gtagcttaaa ttttgctcgc gcactactca gcgacctcca
    11041 acacacaagc agggagcaga tactggctta actatgcggc atcagagcag attgtactga
    11101 gagtgcacca taggggatcg ggagatctcc cgatccgtcg acgtcaggtg gc
//
