LOCUS Exported 11755 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pAcGP67A - murine TfR SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11755) TITLE Bjorkman lab plasmids REFERENCE 2 (bases 1 to 11755) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11755 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 87..106 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 300..322 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 314..331 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 315..331 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" misc_recomb 3504..4331 /label=baculovirus recombination region (lef2/ORF603) /note="contains ORF603 and part of lef2" CDS 3504..3628 /codon_start=3 /product="baculovirus late expression factor 2" /label=lef2 /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY" CDS complement(3666..4271) /codon_start=1 /product="baculovirus ORF603 protein" /label=ORF603 /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR" promoter 4335..4426 /gene="polh from Autographa californica" /label=polyhedrin promoter /note="promoter for the baculovirus polyhedrin gene" primer_bind 4351..4368 /label=Polyhedrin-F /note="Polyhedrin promoter, forward primer" sig_peptide 4523..4582 /gene="Autographa californica gp64" /product="signal sequence from the baculovirus envelope glycoprotein GP64" /label=GP64 signal sequence CDS 4592..4609 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" CDS 4619..4630 /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" misc_recomb 7097..7802 /label=baculovirus recombination region (ORF1629) /note="contains part of ORF1629" 3'UTR 7185..7557 /label=polyhedrin 3' UTR /note="3' UTR from the Autographa californica nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)" CDS complement(7214..7802) /codon_start=2 /product="baculovirus capsid-associated protein" /label=ORF1629 /note="required for viral replication" /translation="IPKSSTTNLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNR PNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKAR LLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIF ALTYHPKDYKFNELLKYVQQLSVNQQRTESSA" CDS complement(8236..8253) /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" primer_bind complement(9470..9486) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(9470..9486) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(9483..9505) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 9494..9510 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(9518..9548) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 9563..9584 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(9701..9718) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(9872..10460) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(9952..9971) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(10631..11491) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 11254..11273 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(11492..11596) /gene="bla" /label=AmpR promoter primer_bind 11664..11682 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" primer_bind complement(11720..11742) /label=pGEX 3' /note="pGEX vectors, reverse primer" ORIGIN 1 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg 61 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca 121 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc 181 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg 241 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc 301 ccagtcacga cgttgtaaaa cgacggccag tgccaagctt ggctcgtaaa gcgagttgaa 361 ggatcatatt tagttgcgtt tatgagataa gattgaaagc acgtgtaaaa tgtttcccgc 421 gcgttggcac aactatttac aatgcggcca agttataaaa gattctaatc tgatatgttt 481 taaaacacct ttgcggcccg agttgtttgc gtacgtgact agcgaagaag atatgtggac 541 cgcagaacag atagtaaaac aaaaccctag tattggagca ataatcgatt taaccaacac 601 gtctaaatat tatgatggtg tgcatttttt gcgggcgggc ctgttataca aaaaaattca 661 agtacctggc cagactttgc cgcctgaaag catagttcaa gaatttattg acacggtaaa 721 agaatttaca gaaaagtgtc ccggcatgtt ggtgggcgtg cactgcacac acggtattaa 781 tcgcaccggt tacatggtgt gcagatattt aatgcacacc ctgggtattg cgccgcagga 841 agccatagat agattcgaaa aagccagagg tcacaaaatt gaaagacaaa attacgttca 901 agatttatta atttaattaa tattatttgc attctttaac aaatacttta tcctattttc 961 aaattgttgc gcttcttcca gcgaaccaaa actatgcttc gcttgctccg tttagcttgt 1021 agccgatcag tggcgttgtt ccaatcgacg gtaggattag gccggatatt ctccaccaca 1081 atgttggcaa cgttgatgtt acgtttatgc ttttggtttt ccacgtacgt cttttggccg 1141 gtaatagccg taaacgtagt gccgtcgcgc gtcacgcaca acaccggatg tttgcgcttg 1201 tccgcggggt attgaaccgc gcgatccgac aaatccacca ctttggcaac taaatcggtg 1261 acctgcgcgt cttttttctg cattatttcg tctttctttt gcatggtttc ctggaagccg 1321 gtgtacatgc ggtttagatc agtcatgacg cgcgtgacct gcaaatcttt ggcctcgatc 1381 tgcttgtcct tgatggcaac gatgcgttca ataaactctt gttttttaac aagttcctcg 1441 gttttttgcg ccaccaccgc ttgcagcgcg tttgtgtgct cggtgaatgt cgcaatcagc 1501 ttagtcacca actgtttgct ctcctcctcc cgttgtttga tcgcgggatc gtacttgccg 1561 gtgcagagca cttgaggaat tacttcttct aaaagccatt cttgtaattc tatggcgtaa 1621 ggcaatttgg acttcataat cagctgaatc acgccggatt tagtaatgag cactgtatgc 1681 ggctgcaaat acagcgggtc gccccttttc acgacgctgt tagaggtagg gcccccattt 1741 tggatggtct gctcaaataa cgatttgtat ttattgtcta catgaacacg tatagcttta 1801 tcacaaactg tatattttaa actgttagcg acgtccttgg ccacgaaccg gacctgttgg 1861 tcgcgctcta gcacgtaccg caggttgaac gtatcttctc caaatttaaa ttctccaatt 1921 ttaacgcgag ccattttgat acacgtgtgt cgattttgca acaactattg ttttttaacg 1981 caaactaaac ttattgtggt aagcaataat taaatatggg ggaacatgcg ccgctacaac 2041 actcgtcgtt atgaacgcag acggcgccgg tctcggcgca agcggctaaa acgtgttgcg 2101 cgttcaacgc ggcaaacatc gcaaaagcca atagtacagt tttgatttgc atattaacgg 2161 cgatttttta aattatctta tttaataaat agttatgacg cctacaactc cccgcccgcg 2221 ttgactcgct gcacctcgag cagttcgttg acgccttcct ccgtgtggcc gaacacgtcg 2281 agcgggtggt cgatgaccag cggcgtgccg cacgcgacgc acaagtatct gtacaccgaa 2341 tgatcgtcgg gcgaaggcac gtcggcctcc aagtggcaat attggcaaat tcgaaaatat 2401 atacagttgg gttgtttgcg catatctatc gtggcgttgg gcatgtacgt ccgaacgttg 2461 atttgcatgc aagccgaaat taaatcattg cgattagtgc gattaaaacg ttgtacatcc 2521 tcgcttttaa tcatgccgtc gattaaatcg cgcaatcgag tcaagtgatc aaagtgtgga 2581 ataatgtttt ctttgtattc ccgagtcaag cgcagcgcgt attttaacaa actagccatc 2641 ttgtaagtta gtttcattta atgcaacttt atccaataat atattatgta tcgcacgtca 2701 agaattaaca atgcgcccgt tgtcgcatct caacacgact atgatagaga tcaaataaag 2761 cgcgaattaa atagcttgcg acgcaacgtg cacgatctgt gcacgcgttc cggcacgagc 2821 tttgattgta ataagttttt acgaagcgat gacatgaccc ccgtagtgac aacgatcacg 2881 cccaaaagaa ctgccgacta caaaattacc gagtatgtcg gtgacgttaa aactattaag 2941 ccatccaatc gaccgttagt cgaatcagga ccgctggtgc gagaagccgc gaagtatggc 3001 gaatgcatcg tataacgtgt ggagtccgct cattagagcg tcatgtttag acaagaaagc 3061 tacatattta attgatcccg atgattttat tgataaattg accctaactc catacacggt 3121 attctacaat ggcggggttt tggtcaaaat ttccggactg cgattgtaca tgctgttaac 3181 ggctccgccc actattaatg aaattaaaaa ttccaatttt aaaaaacgca gcaagagaaa 3241 catttgtatg aaagaatgcg tagaaggaaa gaaaaatgtc gtcgacatgc tgaacaacaa 3301 gattaatatg cctccgtgta taaaaaaaat attgaacgat ttgaaagaaa acaatgtacc 3361 gcgcggcggt atgtacagga agaggtttat actaaactgt tacattgcaa acgtggtttc 3421 gtgtgccaag tgtgaaaacc gatgtttaat caaggctctg acgcatttct acaaccacga 3481 ctccaagtgt gtgggtgaag tcatgcatct tttaatcaaa tcccaagatg tgtataaacc 3541 accaaactgc caaaaaatga aaactgtcga caagctctgt ccgtttgctg gcaactgcaa 3601 gggtctcaat cctatttgta attattgaat aataaaacaa ttataaatgt caaatttgtt 3661 ttttattaac gatacaaacc aaacgcaaca agaacatttg tagtattatc tataattgaa 3721 aacgcgtagt tataatcgct gaggtaatat ttaaaatcat tttcaaatga ttcacagtta 3781 atttgcgaca atataatttt attttcacat aaactagacg ccttgtcgtc ttcttcttcg 3841 tattccttct ctttttcatt tttctcctca taaaaattaa catagttatt atcgtatcca 3901 tatatgtatc tatcgtatag agtaaatttt ttgttgtcat aaatatatat gtctttttta 3961 atggggtgta tagtaccgct gcgcatagtt tttctgtaat ttacaacagt gctattttct 4021 ggtagttctt cggagtgtgt tgctttaatt attaaattta tataatcaat gaatttggga 4081 tcgtcggttt tgtacaatat gttgccggca tagtacgcag cttcttctag ttcaattaca 4141 ccatttttta gcagcaccgg attaacataa ctttccaaaa tgttgtacga accgttaaac 4201 aaaaacagtt cacctccctt ttctatacta ttgtctgcga gcagttgttt gttgttaaaa 4261 ataacagcca ttgtaatgag acgcacaaac taatatcaca aactggaaat gtctatcaat 4321 atatagttgc tgatatcatg gagataatta aaatgataac catctcgcaa ataaataagt 4381 attttactgt tttcgtaaca gttttgtaat aaaaaaacct ataaatattc cggattattc 4441 ataccgtccc accatcgggc gcggatctat gctactagta aatcagtcac accaaggctt 4501 caataaggaa cacacaagca agatggtaag cgctattgtt ttatatgtgc ttttggcggc 4561 ggcggcgcat tctgcctttg cggcggatcc gcatcatcat catcatcaca gcagcggcat 4621 cgagggacgt ggtgaattcc gcttatattg ggcagacctc aaaacactgt tgtcagagaa 4681 gttgaactcc atagagtttg ctgacaccat caagcagctg agccagaata catacactcc 4741 tcgtgaggct ggatctcaaa aagatgaaag tcttgcctat tatattgaaa atcagttcca 4801 tgaatttaaa ttcagcaaag tctggcgaga tgaacactat gtgaagattc aagtgaaaag 4861 cagcattggt caaaacatgg tgaccatagt gcagtcaaat ggtaacttag acccagtgga 4921 gtctcccgag ggttatgtgg cattcagtaa acctacagaa gtttctggta aactggtcca 4981 tgctaatttt ggcactaaaa aggactttga agaactaagt tattctgtga atggatcttt 5041 agtgattgtt agagcagggg aaattacttt tgcagaaaag gttgcaaatg cccaaagctt 5101 taatgcaatt ggtgtcctca tatacatgga caagaataaa ttccccgttg ttgaggcaga 5161 ccttgcactc tttggacatg ctcatctagg aactggtgat ccatacacac ctggctttcc 5221 ttctttcaat catactcagt ttccgccatc tcagtcatca gggttgccta atatacctgt 5281 gcaaacaatc tcaagagctg ctgcagaaaa gctatttgga aaaatggaag gaagctgtcc 5341 tgctagatgg aacatagatt cttcatgtaa gctggaactt tcacagaatc aaaatgtgaa 5401 gctcattgtg aaaaacgtac tgaaagaaag aagaatactt aacatctttg gagttattaa 5461 aggttatgag gaaccagacc gttatgttgt agtaggagcc cagagagacg ctttgggtgc 5521 tggtgttgcg gcgaagtcca gtgtgggaac aggtcttctg ttgaaacttg cccaagtatt 5581 ctcagatatg atttcaaaag atggatttag acccagcaga agtataatct ttgccagctg 5641 gactgcaggc gactttggag ctgttggtgc cactgagtgg ttggagggat acctttcatc 5701 tttgcattta aaagctttca cttatattaa tttggataaa gttgtccttg gtactagtaa 5761 cttcaaagtt tctgccagcc ccttattata tacacttatg ggaaagataa tgcaagatgt 5821 aaagcatcca gttgatggaa aatctctata tagagacagc aattggatta gcaaagttga 5881 gaaactttcc tttgacaatg ctgcatatcc tttccttgca tattctggaa tcccagcagt 5941 ttctttttgt ttttgtgagg atgcagacta tccttatttg ggcactagat tggataccta 6001 tgaggcattg actcagaaag ttcctcagct caaccaaatg gttcgtacag cagcggaagt 6061 ggctggtcag ctcattatta aacttaccca tgacgttgaa ttgaacctgg actatgagat 6121 gtataacagc aaactactgt catttatgaa ggatctgaac cagttcaaaa cagatatcag 6181 ggatatgggt ctaagtctac agtggctgta ttccgctcgt ggagactact tccgtgctac 6241 ttctagacta acaactgatt ttcataatgc tgagaaaaca aacagatttg tcatgaggga 6301 aatcaatgat cgtattatga aagtggagta tcacttcctg tcgccctatg tatctccaag 6361 agagtctcct ttccgacata tcttctgggg ctctggctct cacactctct cagctttagt 6421 ggagaacttg aagcttcgtc aaaaaaatat tactgctttt aatgaaaccc tcttcagaaa 6481 ccagttggcc ctggctactt ggactattca gggagtcgca aatgccctct ctggtgacat 6541 ttggaatatt gacaatgagt tttaaatgta acatgcataa ttaaataaga gcagggtagt 6601 ccggccgctg cagatctgat cctttcctgg gacccggcaa gaaccaaaaa ctcactctct 6661 tcaaggaaat ccgtaatgtt aaacccgaca cgatgaagct tgtcgttgga tggaaaggaa 6721 aagagttcta cagggaaact tggacccgct tcatggaaga cagcttcccc attgttaacg 6781 accaagaagt gatggatgtt ttccttgttg tcaacatgcg tcccactaga cccaaccgtt 6841 gttacaaatt cctggcccaa cacgctctgc gttgcgaccc cgactatgta cctcatgacg 6901 tgattaggat cgtcgagcct tcatgggtgg gcagcaacaa cgagtaccgc atcagcctgg 6961 ctaagaaggg cggcggctgc ccaataatga accttcactc tgagtacacc aactcgttcg 7021 aacagttcat cgatcgtgtc atctgggaga acttctacaa gcccatcgtt tacatcggta 7081 ccgactctgc tgaagaggag gaaattctcc ttgaagtttc cctggtgttc aaagtaaagg 7141 agtttgcacc agacgcacct ctgttcactg gtccggcgta ttaaaacacg atacattgtt 7201 attagtacat ttattaagcg ctagattctg tgcgttgttg atttacagac aattgttgta 7261 cgtattttaa taattcatta aatttataat ctttagggtg gtatgttaga gcgaaaatca 7321 aatgattttc agcgtcttta tatctgaatt taaatattaa atcctcaata gatttgtaaa 7381 ataggtttcg attagtttca aacaagggtt gtttttccga accgatggct ggactatcta 7441 atggattttc gctcaacgcc acaaaacttg ccaaatcttg tagcagcaat ctagctttgt 7501 cgatattcgt ttgtgttttg ttttgtaata aaggttcgac gtcgttcaaa atattatgcg 7561 cttttgtatt tctttcatca ctgtcgttag tgtacaattg actcgacgta aacacgttaa 7621 ataaagcttg gacatattta acatcgggcg tgttagcttt attaggccga ttatcgtcgt 7681 cgtcccaacc ctcgtcgtta gaagttgctt ccgaagacga ttttgccata gccacacgac 7741 gcctattaat tgtgtcggct aacacgtccg cgatcaaatt tgtagttgag ctttttggaa 7801 ttatttctga ttgcgggcgt ttttgggcgg gtttcaatct aactgtgccc gattttaatt 7861 cagacaacac gttagaaagc gatggtgcag gcggtggtaa catttcagac ggcaaatcta 7921 ctaatggcgg cggtggtgga gctgatgata aatctaccat cggtggaggc gcaggcgggg 7981 ctggcggcgg aggcggaggc ggaggtggtg gcggtgatgc agacggcggt ttaggctcaa 8041 atgtctcttt aggcaacaca gtcggcacct caactattgt actggtttcg ggcgccgttt 8101 ttggtttgac cggtctgaga cgagtgcgat ttttttcgtt tctaatagct tccaacaatt 8161 gttgtctgtc gtctaaaggt gcagcgggtt gaggttccgt cggcattggt ggagcgggcg 8221 gcaattcaga catcgatggt ggtggtggtg gtggaggcgc tggaatgtta ggcacgggag 8281 aaggtggtgg cggcggtgcc gccggtataa tttgttctgg tttagtttgt tcgcgcacga 8341 ttgtgggcac cggcgcaggc gccgctggct gcacaacgga aggtcgtctg cttcgaggca 8401 gcgcttgggg tggtggcaat tcaatattat aattggaata caaatcgtaa aaatctgcta 8461 taagcattgt aatttcgcta tcgtttaccg tgccgatatt taacaaccgc tcaatgtaag 8521 caattgtatt gtaaagagat tgtctcaagc tcgcacgccg ataacaagcc ttttcatttt 8581 tactacagca ttgtagtggc gagacacttc gctgtcgtcg acgtacatgt atgctttgtt 8641 gtcaaaaacg tcgttggcaa gctttaaaat atttaaaaga acatctctgt tcagcaccac 8701 tgtgttgtcg taaatgttgt ttttgataat ttgcgcttcc gcagtatcga cacgttcaaa 8761 aaattgatgc gcatcaattt tgttgttcct attattgaat aaataagatt gtacagattc 8821 atatctacga ttcgtcatgg ccaccacaaa tgctacgctg caaacgctgg tacaatttta 8881 cgaaaactgc aaaaacgtca aaactcggta taaaataatc aacgggcgct ttggcaaaat 8941 atctatttta tcgcacaagc ccactagcaa attgtatttg cagaaaacaa tttcggcgca 9001 caattttaac gctgacgaaa taaaagttca ccagttaatg agcgaccacc caaattttat 9061 aaaaatctat tttaatcacg gttccatcaa caaccaagtg atcgtgatgg actacattga 9121 ctgtcccgat ttatttgaaa cactacaaat taaaggcgag ctttcgtacc aacttgttag 9181 caatattatt agacagctgt gtgaagcgct caacgatttg cacaagcaca atttcataca 9241 caacgacata aaactcgaaa atgtcttata tttcgaagca cttgatcgcg tgtatgtttg 9301 cgattacgga ttgtgcaaac acgaaaactc acttagcgtg cacgacggca cgttggagta 9361 ttttagtccg gaaaaaattc gacaccacaa ctatgcacgt tcgtttgact ggtacgccgt 9421 cggcgtgtta acatacaagt tgctaaccgg cggccaattc gtaatcatgg tcatagctgt 9481 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa 9541 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac 9601 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg 9661 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc 9721 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat 9781 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 9841 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 9901 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 9961 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 10021 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta 10081 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 10141 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 10201 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 10261 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat 10321 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 10381 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 10441 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 10501 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 10561 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 10621 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc 10681 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac 10741 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat 10801 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg 10861 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata 10921 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta 10981 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 11041 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 11101 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 11161 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc 11221 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt 11281 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 11341 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 11401 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 11461 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 11521 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 11581 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta 11641 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt 11701 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagc //