LOCUS Exported 3244 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Provides constitutive promoter cassette between attL4-attR1 on Gateway entry vector. ACCESSION . VERSION . KEYWORDS pENTR-L4R1-PGK SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3244) TITLE Minoru Ko Lab plasmids REFERENCE 2 (bases 1 to 3244) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3244 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 593..688 /gene="mutant version of attL" /label=attL4 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" promoter 708..1207 /label=PGK promoter /note="mouse phosphoglycerate kinase 1 promoter" primer_bind complement(743..766) /label=MSCV-rev /note="Murine stem cell virus, reverse primer" primer_bind 1140..1159 /label=mPGK-F /note="Mouse PGK promoter, forward primer" promoter 1219..1266 /label=EM7 promoter /note="synthetic bacterial promoter " protein_bind 1289..1413 /gene="mutant version of attR" /label=attR1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1485..1504) /label=T7 /note="T7 promoter, forward primer" promoter complement(1486..1504) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1509..1525) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1509..1525) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1627..1646) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 1638..2447 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(1705..1724) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 2594..3182 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3083..3102 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccga gttaacgcta ccatggagct ccaaataatg 601 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca atgctttttt 661 ataatgccaa ctttgtatag aaaagttgtt taaacgtcga ctctaccggg taggggaggc 721 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta 781 cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc 841 gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc 901 cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct 961 cgtgcagatg gacggcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca 1021 atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg 1081 cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc 1141 attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg 1201 cctttcgacc tgcagcctgt tgacaattaa tcatcggcat agtatatcgg catagtataa 1261 tacgacaaga tgaggaacta aaccgaattc aagtttgtac aaaaaagttg aacgagaaac 1321 gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac agactacata 1381 atactgtaaa acacaacata tgcagtcact atgaaccaac tacttagatg gtattagtga 1441 cctgtagaat tcgagctcta gagctgcagg gcggccgcga tatcccctat agtgagtcgt 1501 attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt 1561 acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca 1621 gtaatacaag gggtgttatg agccatattc aacgggaaac gtcgaggccg cgattaaatt 1681 ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag 1741 gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg 1801 gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg 1861 aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac 1921 tcaccactgc gatccccgga aaaacagcat tccaggtatt agaagaatat cctgattcag 1981 gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt 2041 gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga 2101 ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac 2161 aagtctggaa agaaatgcat aaacttttgc cattctcacc ggattcagtc gtcactcatg 2221 gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg 2281 ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg 2341 gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg 2401 atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatca gaattggtta 2461 attggttgta acactggcag agcattacgc tgacttgacg ggacggcgca agctcatgac 2521 caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga 2581 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa 2641 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga 2701 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt 2761 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt 2821 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat 2881 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct 2941 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca 3001 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag 3061 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc 3121 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga 3181 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca 3241 tgtt //