LOCUS Exported 7030 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Reporter plasmid with a 12-nucleotide specific ""barcode"" sequence. This plasmid is not available as an individual bacterial stab, but can be requested as part of the Plasmid-ID Kit (Kit #1000000168 ).. ACCESSION . VERSION . KEYWORDS pOPS0502 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7030) AUTHORS Mendoza-Suarez MA, Geddes BA, Sanchez-Canizares C, Ramirez-Gonzalez RH, Kirchhelle C, Jorrin B, Poole PS TITLE Optimizing Rhizobium-legume symbioses by simultaneous measurement of rhizobial competitiveness and N2 fixation in nodules. JOURNAL Proc Natl Acad Sci U S A. 2020 May 5;117(18):9822-9831. doi: 10.1073/pnas.1921225117. Epub 2020 Apr 21. PUBMED 32317381 REFERENCE 2 (bases 1 to 7030) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7030 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 9..103 /label=lambda t0 terminator /note="transcription terminator from phage lambda" primer_bind complement(202..221) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 213..1028 /codon_start=1 /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" oriT 1179..1287 /note="incP origin of transfer" CDS complement(1348..2496) /codon_start=1 /product="trans-acting replication protein that binds to and activates oriV" /label=trfA /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS IVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR" rep_origin 2889..3420 /label=oriV /note="incP origin of replication" terminator 5942..6028 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" CDS 6275..6988 /codon_start=1 /product="GFP variant that folds robustly even when fused to poorly folded proteins (Pedelacq et al., 2006)" /label=superfolder GFP /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE FVTAAGITHGMDELYK" primer_bind complement(7008..7030) /label=M13/pUC Forward /note="In lacZ gene" ORIGIN 1 cgctcttgga ctcctgttga tagatccagt aatgacctca gaactccatc tggatttgtt 61 cagaacgctc ggttgccgcc gggcgttttt tattggtgag aatccaggca attgtgtctc 121 aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgaaca ataaaactgt 181 ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcagcgt gaaacgagct 241 gtagccgtcc gcgtctgaac agcaacatgg atgcggatct gtatggctat aaatgggcgc 301 gtgataacgt gggtcagagc ggcgcgacca tttatcgtct gtatggcaaa ccggatgcgc 361 cggaactgtt tctgaaacat ggcaaaggca gcgtggcgaa cgatgtgacc gatgaaatgg 421 tgcgtctgaa ctggctgacc gaatttatgc cgctgccgac cattaaacat tttattcgca 481 ccccggatga tgcgtggctg ctgaccaccg cgattccggg caaaaccgcg tttcaggtgc 541 tggaagaata tccggatagc ggcgaaaaca ttgtggatgc gctggccgtg tttctgcgtc 601 gtctgcatag cattccggtg tgcaactgcc cgtttaacag cgatcgtgtg tttcgtctgg 661 cccaggcgca gagccgtatg aacaacggcc tggtggatgc gagcgatttt gatgatgaac 721 gtaacggctg gccggtggaa caggtgtgga aagaaatgca taaactgctg ccgtttagcc 781 cggatagcgt ggtgacccac ggcgatttta gcctggataa cctgattttc gatgaaggca 841 aactgattgg ctgcattgat gtgggccgtg tgggcattgc ggatcgttat caggatctgg 901 ccattctgtg gaactgcctg ggcgaattta gcccgagcct gcaaaaacgt ctgtttcaga 961 aatatggcat tgataatccg gatatgaaca aactgcaatt tcatctgatg ctggatgaat 1021 ttttctaata attaattgaa ctacttttcc gctgcataac cctgcttcgg ggtcattata 1081 gcgatttttt cggtatatcc atcctttttc gcacgatata caggattttg ccaaagggtt 1141 cgtgtagact ttccttggtg tatccaacgg cgtcagccgg gcaggatagg tgaagtaggc 1201 ccacccgcga gcgggtgttc cttcttcact gtcccttatt cgcacctggc ggtgctcaac 1261 gggaatcctg ctctgcgagg ctggccgtag gccggccgcg atgcaggtgg ctgctgaacc 1321 cccagccgga actgacccca caaggcccta gcgtttgcaa tgcaccaggt catcattgac 1381 ccaggcgtgt tccaccaggc cgctgcctcg caactcttcg caggcttcgc cgacctgctc 1441 gcgccacttc ttcacgcggg tggaatccga tccgcacatg aggcggaagg tttccagctt 1501 gagcgggtac ggctcccggt gcgagctgaa atagtcgaac atccgtcggg ccgtcggcga 1561 cagcttgcgg tacttctccc atatgaattt cgtgtagtgg tcgccagcaa acagcacgac 1621 gatttcctcg tcgatcagga cctggcaacg ggacgttttc ttgccacggt ccaggacgcg 1681 gaagcggtgc agcagcgaca ccgattccag gtgcccaacg cggtcggacg tgaagcccat 1741 cgccgtcgcc tgtaggcgcg acaggcattc ctcggccttc gtgtaatacc ggccattgat 1801 cgaccagccc aggtcctggc aaagctcgta gaacgtgaag gtgatcggct cgccgatagg 1861 ggtgcgcttc gcgtactcca acacctgctg ccacaccagt tcgtcatcgt cggcccgcag 1921 ctcgacgccg gtgtaggtga tcttcacgtc cttgttgacg tggaaaatga ccttgttttg 1981 cagcgcctcg cgcgggattt tcttgttgcg cgtggtgaac agggcagagc gggccgtgtc 2041 gtttggcatc gctcgcatcg tgtccggcca cggcgcaata tcgaacaagg aaagctgcat 2101 ttccttgatc tgctgcttcg tgtgtttcag caacgcggcc tgcttggctt cgctgacctg 2161 ttttgccagg tcctcgccgg cggtttttcg cttcttggtc gtcatagttc ctcgcgtgtc 2221 gatggtcatc gacttcgcca aacctgccgc ctcctgttcg agccgacgcg aacgctccac 2281 ggcggccgat ggcgcgggca gggcaggggg agccagttgc acgctgtcgc gctcgatctt 2341 ggccgtagct tgctggacta tcgagccgac ggactggaag gtttcgcggg gcgcacgcat 2401 gacggtgcgg cttgcgatgg tttcggcatc ctcggcggaa aaccccgcgt cgatcagttc 2461 ttgcctgtat gccttccggt caaacgtccg attcattcac cctccttgcg ggattgcccc 2521 ggaattaatt ccccggatcg atccgtcgat cttgatcccc tgcgccatca gatccttggc 2581 ggcaagaaag ccatccagtt tactttgcag ggcttcccaa ccttaccaga gggcgcccca 2641 gctggcaatt ccggttcgct tgctgtccat aaaaccgccc agtctagcta tcgccatgta 2701 agcccactgc aagctacctg ctttctcttt gcgcttgcgt tttcccttgt ccagatagcc 2761 cagtagctga cattcatccg gggtcagcac cgtttctgcg gactggcttt ctacgtggct 2821 gccatttttg gggtgaggcc gttcgcggcc gaggggcgca gcccctgggg ggatgggagg 2881 cccgcgttag cgggccggga gggttcgaga agggggggca ccccccttcg gcgtgcgcgg 2941 tcacgcgcac agggcgcagc cctggttaaa aacaaggttt ataaatattg gtttaaaagc 3001 aggttaaaag acaggttagc ggtggccgaa aaacgggcgg aaacccttgc aaatgctgga 3061 ttttctgcct gtggacagcc cctcaaatgt caataggtgc gcccctcatc tgtcagcact 3121 ctgcccctca agtgtcaagg atcgcgcccc tcatctgtca gtagtcgcgc ccctcaagtg 3181 tcaataccgc agggcactta tccccaggct tgtccacatc atctgtggga aactcgcgta 3241 aaatcaggcg ttttcgccga tttgcgaggc tggccagctc cacgtcgccg gccgaaatcg 3301 agcctgcccc tcatctgtca acgccgcgcc gggtgagtcg gcccctcaag tgtcaacgtc 3361 cgcccctcat ctgtcagtga gggccaagtt ttccgcgagg tatccacaac gccggcggcc 3421 ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga tcccccgcag 3481 aaaaaaagga tctcaagaag atcctttgat cttttctaca ttactgcatg agcttgtgga 3541 agtgtgctga gcataaatga ttctcctagc tgttctttgg gtaagtacgc catcaggacg 3601 ttgtgagtgg cgcgattttt agcggctgaa atcagccctt gagcctgtcg gcaagtcgcg 3661 tcatgaggtc catgcgctca tgcaggatcg ccacgaccaa cgcgggttcg cccgcacgcg 3721 gcaggcaaaa aacgtagtgg tgttcgcagc gggccatccg cagcgcggga aagagttcgc 3781 tcatgtcctt aaacgggcct tcgccggcgg caagcctggc tatgccctgt tccagcttag 3841 cgatatagcg gcgcacctgc gccgcgcccc actcccggcg cgtgtagcgg atgatgccgc 3901 gtagatcggc ttcggcctca gccgtgagga tgtaggccgt caagcgcgat ccccgctgag 3961 ttcttcatca agaatttcgc cgacgctctt ggtggacacc ttgccggcaa gcccatcgtt 4021 gatgcggttc cccagcatgg ttttcagttc ctgccatgcc tgatcggcat cagcgtcacc 4081 ggggaacaga cgttcgaggg cgtattgctt aatggtcttg ccctgcaagg cggccagggc 4141 tttcaggctc tggtgctgct ggtccgtcat gtcgattgtc aggcggctca ttggataacc 4201 tccataaaat acacgtaacc acattagcac atatgtgggc gtgaggctac agcgcgaggc 4261 gcattaaggt cgggaaaatg cgctaggcgc atttaaattg cgtattgctg taatgcgcca 4321 tgccggctag actaggccca aatgggtata cccaatttga ccaaggggga cgcgatgagg 4381 gcggccaagc actaccgaca acttctatcc atcgacttca acatcgaggc gctggccttc 4441 gtgcctggac ccgacggcac acgcggccgg cgcatccacg tcctggggcg cgaggtccgc 4501 gaccggcccg gcctggtcga gtacctttcg ccggcgttcg gctcgcgggt ggcgctggac 4561 ggctactgca aggccaattt cgatgcagtg ctgcacctgg cgtaccccga tcatcagcaa 4621 tggggccacg catgaagcgc cgaagctacg ccatgctgcg cgccgctgcc gcgctggccg 4681 tcctggtcgt tgcctcgccg gcatgggccg agctgcgcgg cgaggtcgtg cgcatcatcg 4741 acggcgacac catcgacgtg ctggtagaca agcagccggt gcgcgtgcgc ctggtggaca 4801 ttgacgcgcc ggaaaagcgg caagccttcg gcgaacgtgc gcgccaggcg ctggccggca 4861 tggtgttccg ccggcacgtc ctggtcgacg agaaggacac cgaccgttac ggccgcacgc 4921 tgggcaccgt ctgggtcaac atggagctgg ccagccggcc gccgcagccg cgcaacgtca 4981 acgccgcgat ggttcaccag ggcatggcgt gggcctatcg cttccacggc cgcgcggccg 5041 accctgaaat gctgcggctc gaacaggagg cgcgaggcaa gcgcgtcggc ctctggtccg 5101 atccgcacgc cgtcgagccg tggaaatggc gacgcgagag caacaaccgg agggacgaag 5161 gttgaaggtc gcccgcatct acctgcgcgc cagtacggac gagcagaatc ttgaacgcca 5221 ggagagcctt gtagcggcca cgcgggccgc cgggtactac gtcgccggca tctaccgcga 5281 gaaggcgtcc ggcgcacgcg ccgaccggcc cgagctgctg cgcatgatcg cggacctgca 5341 acctggtgaa gtcgtcgttg cggagaagat cgaccgcatc agccgcttgc cgttggccga 5401 ggccgagcgc ctggttgcgt cgatccgggc caaaggggcc aagctggccg tgcctggcgt 5461 ggtggacctg tcggagctgg ccgccgaggc gaacggagtg gcgaaaatcg ttctggaatc 5521 cgtccaggac atgcttttga agctcgcctt gcagatggcc cgcgacgact acgaggatcg 5581 gcgcgagcgt caacgtcagg gtgtccagtt ggcgaaggcc gccggccgct acaccggccg 5641 caaacgtgac gccggcatgc acgaccgcat catcacgctt cgctccggcg gatcgagcat 5701 tgccaagacg gccaagctgg tcggatgcag cccgagccag gtcaaacgag tgtgggcggc 5761 ctggaacgcg cagcagcaaa aataaagccg ggcagtgccc ggcttttctc accttttcgc 5821 gtcccgcagg gccgctgcga gcgccctacc tagatcctcg ctttccccct cggtgtagtc 5881 cggccagggc acgaagggcg cggatgcgaa cctgttgagc aggtcagatc tagggcggcg 5941 gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt 6001 ctttcgactg agcctttcgt tttatttgat gcctgccgaa ttcggatccg gagctcgaca 6061 cgacattgtc gtcacctttg tcgggtccgc gacaagcttt ttgttcctcg atcccgtatg 6121 ttttcgccta aatatctgaa acagcagcaa attatttttc gttagctcaa tcggcccact 6181 tggcacgagt cttgaaattt attgcgaggc ggcggagctg ccggccgcat ccgtgcggca 6241 taaccacggt tgtgaacaaa tgaaggaagg ccaaatgcgt aaaggcgaag agctgttcac 6301 tggtgtcgtc cctattctgg tggaactgga tggtgatgtc aacggtcata agttttccgt 6361 gcgtggcgag ggtgaaggtg acgcaactaa tggtaaactg acgctgaagt tcatctgtac 6421 tactggtaaa ctgccggtac cttggccgac tctggtaacg acgctgactt atggtgttca 6481 gtgctttgct cgttatccgg accatatgaa gcagcatgac ttcttcaagt ccgccatgcc 6541 ggaaggctat gtgcaggaac gcacgatttc ctttaaggat gacggcacgt acaaaacgcg 6601 tgcggaagtg aaatttgaag gcgataccct ggtaaaccgc attgagctga aaggcattga 6661 ctttaaagaa gatggcaata tcctgggcca taagctggaa tacaatttta acagccacaa 6721 tgtttacatc accgccgata aacaaaaaaa tggcattaaa gcgaatttta aaattcgcca 6781 caacgttgag gatggcagcg tgcagctggc tgatcactac cagcaaaaca ctccaatcgg 6841 tgatggtcct gttctgctgc cagacaatca ctatctgagc acgcaaagcg ttctgtctaa 6901 agatccgaac gagaaacgcg atcatatggt tctgctggag ttcgtaaccg cagcgggcat 6961 cacgcatggt atggatgaac tgtacaaatg agcttaattg tgtcggacgt tttacaacgt 7021 cgtgactggg //