LOCUS Exported 5134 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Localization: Nucleus/Histones, Excitation: 587, Emission: 610. ACCESSION . VERSION . KEYWORDS mCherry-H2B-18 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5134) TITLE Davidson lab - mCherry constructs REFERENCE 2 (bases 1 to 5134) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5134 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 35..338 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 339..542 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 493..513 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" CDS 631..1008 /codon_start=1 /gene="HIST1H2BJ" /product="human histone H2B" /label=H2B /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP GELAKHAVSEGTKAITKYTSAK" regulatory 1057..1066 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 1063..1773 /codon_start=1 /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /label=mCherry /note="mammalian codon-optimized" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" primer_bind complement(1208..1226) /label=mCherry-R /note="mCherry, reverse primer" primer_bind 1476..1495 /label=mCherry-F /note="mCherry, forward primer" polyA_signal 1896..2017 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(1933..1952) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 1987..2006 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(2024..2479) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2161..2182) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 2373..2392 /label=F1ori-R /note="F1 origin, reverse primer" promoter 2506..2610 /gene="bla" /label=AmpR promoter promoter 2612..2969 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(2635..2655) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 2820..2955 /label=SV40 ori /note="SV40 origin of replication" primer_bind 2882..2901 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 3004..3798 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(3058..3077) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 3668..3687 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(3986..4005) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 4030..4077 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 4406..4994 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4895..4914 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc 61 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca 121 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg 181 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg 241 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt 301 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca 361 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg 421 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact 481 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag 541 ctggtttagt gaaccgtcag atccgctagc gctaccggac tcagatctcg agctcaagct 601 tcgaattctg cagtcgacgg taccgccacc atgccagagc cagcgaagtc tgctcccgcc 661 ccgaaaaagg gctccaagaa ggcggtgact aaggcgcaga agaaaggcgg caagaagcgc 721 aagcgcagcc gcaaggagag ctattccatc tatgtgtaca aggttctgaa gcaggtccac 781 cctgacaccg gcatttcgtc caaggccatg ggcatcatga attcgtttgt gaacgacatt 841 ttcgagcgca tcgcaggtga ggcttcccgc ctggcgcatt acaacaagcg ctcgaccatc 901 acctccaggg agatccagac ggccgtgcgc ctgctgctgc ctggggagtt ggccaagcac 961 gccgtgtccg agggtactaa ggccatcacc aagtacacca gcgctaagga tcacggtcac 1021 ggtactggtt ctactggttc tggttcttct ccggtcgcca ccatggtgag caagggcgag 1081 gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc 1141 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc 1201 cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg 1261 tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac 1321 tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac 1381 ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag 1441 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg 1501 ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc 1561 aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac 1621 aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc 1681 acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac 1741 tccaccggcg gcatggacga gctgtacaag taaagcggcc gcgactctag atcataatca 1801 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga 1861 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg 1921 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt 1981 ctagttgtgg tttgtccaaa ctcatcaatg tatcttaagg cgtaaattgt aagcgttaat 2041 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc 2101 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt 2161 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa 2221 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg 2281 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga 2341 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct 2401 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat 2461 gcgccgctac agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt 2521 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa 2581 atgcttcaat aatattgaaa aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg 2641 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca 2701 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa 2761 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca 2821 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt 2881 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag 2941 gcttttttgg aggcctaggc ttttgcaaag atcgatcaag agacaggatg aggatcgttt 3001 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta 3061 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg 3121 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa 3181 ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct 3241 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg 3301 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca 3361 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat 3421 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac 3481 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc 3541 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa 3601 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag 3661 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc 3721 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt 3781 cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca 3841 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa 3901 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct 3961 tcgcccaccc tagggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc 4021 gcgctatgac ggcaataaaa agacagaata aaacgcacgg tgttgggtcg tttgttcata 4081 aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga ccccattggg 4141 gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg ggtgaaggcc 4201 cagggctcgc agccaacgtc ggggcggcag gccctgccat agcctcaggt tactcatata 4261 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt 4321 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc 4381 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct 4441 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa 4501 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag 4561 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc 4621 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg 4681 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca 4741 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat 4801 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg 4861 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc 4921 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc 4981 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc 5041 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg 5101 ccatgcatta gttattaata gtaatcaatt acgg //