LOCUS Exported 722 bp ds-DNA linear SYN 22-MAY-2021 DEFINITION Bacterial expression of pH-sensitive monomeric mNectarine red fluorescent protein. ACCESSION . VERSION . KEYWORDS pBAD-mNectarine SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 722) AUTHORS Johnson DE, Ai HW, Wong P, Young JD, Campbell RE, Casey J TITLE Red fluorescent protein pH biosensor to detect concentrative nucleoside transport. JOURNAL J Biol Chem. 2009 Jun 3. ():. PUBMED 19494110 REFERENCE 2 (bases 1 to 722) AUTHORS . TITLE Direct Submission JOURNAL Exported May 22, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..722 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 85..102 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" CDS 106..138 /codon_start=1 /product="leader peptide from bacteriophage T7 gene 10" /label=T7 tag (gene 10 leader) /note="promotes efficient translation in E. coli" /translation="MASMTGGQQMG" CDS 142..165 /codon_start=1 /product="Xpress(TM) epitope tag, including an enterokinase recognition and cleavage site" /label=Xpress(TM) tag /translation="DLYDDDDK" primer_bind complement(326..344) /label=mCherry-R /note="mCherry, reverse primer" ORIGIN 1 cctgacgctt tttatcgcaa ctctctactg tttctccata cccgtttttt gggctaacag 61 gaggaattaa ccatgggggg ttctcatcat catcatcatc atggtatggc tagcatgact 121 ggtggacagc aaatgggtcg ggatctgtac gacgatgacg ataaggatcc gagctcgagc 181 atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag 241 gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggtga gggcgagggc 301 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc 361 ttcgcctggg acatcctgtc ccctcagttc tgctacggct ccaaggccta tgtgaagcac 421 cccgccgaca tccccgacta cttgaagctg tccttccccg agggcctcaa ttgggagcgc 481 gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac 541 ggcgagttca tctacaaggt gaagctgcgc ggcactaact tcccctccga cggccccgta 601 atgcagtgtc gtaccgtggg ctgggaggcc tccactgagc ggatgcaccc cgaggacggc 661 gccctgaagg gcgagatcat gcagaggctg aagctgaagg gacggcggcc actacgacgc 721 tg //