LOCUS Exported 6846 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Expression of Leishmania donovani ectodomain in mammalian cells. ACCESSION . VERSION . KEYWORDS D26_Ld_200260 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6846) TITLE Leishmania ectodomain expression plasmids REFERENCE 2 (bases 1 to 6846) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6846 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 2154..2173 /label=rbglobpA-R /note="Rabbit beta-globin polyA, reverse primer. Also called rb-glob-pA-term-R" polyA_signal 2172..2227 /label=-beta-globin poly(A) signal /note="rabbit beta-globin polyadenylation signal (Gil and Proudfoot, 1987)" CDS complement(2279..2296) /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" regulatory 3163..3172 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" primer_bind complement(3195..3214) /label=pMT2-F /note="Synthetic intron, forward primer" primer_bind complement(3695..3719) /label=LNCX /note="Human CMV promoter, forward primer" promoter complement(3716..3919) /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind complement(3745..3765) /label=CMV-F /note="Human CMV immediate early promoter, forward primer" enhancer 3920..4299 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" primer_bind complement(4570..4586) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(4570..4586) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(4583..4605) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 4594..4610 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(4618..4648) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 4663..4684 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(4801..4818) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(4972..5560) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(5052..5071) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(5731..6591) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 6354..6373 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(6592..6696) /gene="bla" /label=AmpR promoter primer_bind 6764..6782 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" ORIGIN 1 gatacagatt aggatagcat atactaccca gatatagatt aggatagcat atgctaccca 61 gatatagatt aggatagcct atgctaccca gatataaatt aggatagcat atactaccca 121 gatatagatt aggatagcat atgctaccca gatatagatt aggatagcct atgctaccca 181 gatatagatt aggatagcat atgctaccca gatatagatt aggatagcat atgctatcca 241 gatatttggg tagtatatgc tacccagata taaattagga tagcatatac taccctaatc 301 tctattagga tagcatatgc tacccggata cagattagga tagcatatac tacccagata 361 tagattagga tagcatatgc tacccagata tagattagga tagcctatgc tacccagata 421 taaattagga tagcatatac tacccagata tagattagga tagcatatgc tacccagata 481 tagattagga tagcctatgc tacccagata tagattagga tagcatatgc tatccagata 541 tttgggtagt atatgctacc catggcaaca ttagcccacc gtgctctcag cgacctcgtg 601 aatatgagga ccaacaaccc tgtgcttggc gctcaggcgc aagtgtgtgt aatttgtcct 661 ccagatcgca gcaatcgcgc ccctatcttg gcccgcccac ctacttatgc aggtattccc 721 cggggtgcca ttagtggttt tgtgggcaag tggtttgacc gcagtggtta gcggggttac 781 aatcagccaa gttattacac ccttatttta cagtccaaaa ccgcagggcg gcgtgtgggg 841 gctgacgcgt gcccccactc cacaatttca aaaaaaagag tggccacttg tctttgttta 901 tgggccccat tggcgtggag ccccgtttaa ttttcggggg tgttagagac aaccagtgga 961 gtccgctgct gtcggcgtcc actctctttc cccttgttac aaatagagtg taacaacatg 1021 gttcacctgt cttggtccct gcctgggaca catcttaata accccagtat catattgcac 1081 taggattatg tgttgcccat agccataaat tcgtgtgaga tggacatcca gtctttacgg 1141 cttgtcccca ccccatggat ttctattgtt aaagatattc agaatgtttc attcctacac 1201 tagtatttat tgcccaaggg gtttgtgagg gttatattgg tgtcatagca caatgccacc 1261 actgaacccc ccgtccaaat tttattctgg gggcgtcacc tgaaaccttg ttttcgagca 1321 cctcacatac accttactgt tcacaactca gcagttattc tattagctaa acgaaggaga 1381 atgaagaagc aggcgaagat tcaggagagt tcactgcccg ctccttgatc ttcagccact 1441 gcccttgtga ctaaaatggt tcactaccct cgtggaatcc tgaccccatg taaataaaac 1501 cgtgacagct catggggtgg gagatatcgc tgttccttag gaccctttta ctaaccctaa 1561 ttcgatagca tatgcttccc gttgggtaac atatgctatt gaattagggt tagtctggat 1621 agtatatact actacccggg aagcatatgc tacccgttta gggttaacaa gggggcctta 1681 taaacactat tgctaatgcc ctcttgaggg tccgcttatc ggtagctaca caggcccctc 1741 tgattgacgt tggtgtagcc tcccgtagtc ttcctgggcc cctgggaggt acatgtcccc 1801 cagcattggt gtaagagctt cagccaagag ttacacataa aggcaatgtt gtgttgcagt 1861 ccacagactg caaagtctgc tccaggatga aagccactca gtgttggcaa atgtgcacat 1921 ccatttataa ggatgtcaac tacagtcaga gaaccccttt gtgtttggtc cccccccgtg 1981 tcacatgtgg aacagggccc agttggcaag ttgtaccaac caactgaagg gattacatgc 2041 actgccccgc gaagaagggg cagagatgtc gtagtcaggt ttagttcgtc cggggcgggg 2101 atcgatcctc tagctagaga tctccgaggg atctcgacca aatgatttgc cctcccatat 2161 gtccttccga gtgagagaca caaaaaattc caacacacta ttgcaatgaa aataaatttc 2221 ctttattagc cagaggtcga ggtcggggga tccttaatta atcacgtgtc tagactcaat 2281 ggtggtgatg gtggtggggg cccagagggg ccagaggagg caggttcctg tccctgtggt 2341 tccacagcat cttctgggcg tccaggatgt ggtgcaggct gccgggcgcg cctcccttca 2401 tgttctgcag cacggacatc acgatgggga tgaccaggta catccagtat ttctgcagga 2461 aggaccgttc ctcgacgggc acttcctctt ccaccacttc gccctcggcg tttcggacca 2521 tccgccgctc ggtctggggt ctggcgattg tctttctggc gtcctctgtc tcggtcaggg 2581 cggtcaggtc ctcgtagcgg actttccggg tcactttcag agggtgcacc aggcccactt 2641 tggtgttcag gtgcacggtg gggaacagtt tctgcaccac gctgcggtcg cactcgtcgc 2701 ccttcagcat cttggcgtgg aagaagtttg tctcgctgga cacctggagg cccatcaggg 2761 aggtggacac gccgggcacg atgttgatgt tctcgtgcag caccactgtc ttgctgtcca 2821 tggcctcgaa gccccggatc aggctgcagg gggtgaccac cacggtggtg ctgtgcttgg 2881 gggcaggctt gccttcctgg ttggggtagg cccggtagta caccaggtcg gcagcggcca 2941 gcttgtttct ctcttccatg ctcagggaca cctcgccgcc cagctgtgta gagatccggg 3001 caggggcctg gctgctcagt ctgctgatgg cgaagctgcc gatccgggac cactcgccgt 3061 cgctgcccac tcttctttcc agcacgtagc tctgagacac ggcggtggcg gccgcaccag 3121 acatgcagag cagcagggac atgagtacct gggtctgaaa ctccatggtg gcaaacttgg 3181 acctgggagt ggacacctgt ggagagaaag gcaaagtgga tgtcattgtc actcaagtgt 3241 atggccagat cgggccaggt gaatatcaaa tcctcctcgt ttttggaaac tgacaatctt 3301 agcgcagaag taatgcccgc ttttgagagg gagtactcac cccaacagct ggatctcaag 3361 cctgccacac ctcacctcga ccatccgccg tctcaagacc gcctacttta attacatcat 3421 cagcagcacc tccgccagaa acaaccccga ccgccacccg ctgccgcccg ccacggtgct 3481 cagcctacct tgcgactgtg actggttaga cgcctttctc gagaggtttt ccgatccggt 3541 cgatgcggac tcgctcaggt ccctcggtgg cggagtaccg ttcggaggcc gacgggtttc 3601 cgatccaaga gtactggaaa gaccgcgaag agtttgtcct caaccgcgag cccaacagct 3661 ggccctcgca gacagcgatg cggaagagag tgaggatctg acggttcact aaacgagctc 3721 tgcttatata gacctcccac cgtacacgcc taccgcccat ttgcgtcaac ggggcggggt 3781 tattacgaca ttttggaaag tcccgttgat tttggtgcca aaacaaactc ccattgacgt 3841 caatggggtg gagacttgga aatccccgtg agtcaaaccg ctatccacgc ccattggtgt 3901 actgccaaaa ccgcatcacc atggtaatag cgatgactaa tacgtagatg tactgccaag 3961 taggaaagtc ccgtaaggtc atgtactggg cataatgcca ggcgggccat ttaccgtcat 4021 tgacgtcaat agggggcgga cttggcatat gatacacttg atgtactgcc aagtgggcag 4081 tttaccgtaa atactccacc cattgacgtc aatggaaagt ccctattggc gttactatgg 4141 gaacatacgt cattattgac gtcaatgggc gggggtcgtt gggcggtcag ccaggcgggc 4201 catttaccgt aagttatgta acgcggaact ccatatatgg gctatgaact aatgaccccg 4261 taattgatta ctattaataa ctagtcaata atcaatgtca acatggcggt catattggac 4321 atgagccaat ataaatgtac atattatgat atagatacaa cgtatgcaat ggccaatagc 4381 caatattgat ttatgctata taaccaatga ctaatatggc taattgccaa tattgattca 4441 atgtatagat cttccatacc taccagttct gcgcctgcag caatggcaac aacgttgccc 4501 ggatctgcga tgataagctg tcaaacatga gaattggtcg acctctagct agagcttggc 4561 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa 4621 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac 4681 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca 4741 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc 4801 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc 4861 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc 4921 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag 4981 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc 5041 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 5101 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 5161 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 5221 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 5281 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 5341 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 5401 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 5461 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt 5521 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 5581 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt 5641 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta 5701 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat 5761 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac 5821 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg 5881 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag 5941 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt 6001 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt 6061 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt 6121 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt 6181 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct 6241 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt 6301 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac gggataatac 6361 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa 6421 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa 6481 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca 6541 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct 6601 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga 6661 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc 6721 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag 6781 gccctttcgt cttcaagaaa attctcatgt ttgacagctt atcatgagga tagcatatgc 6841 tacccg //