LOCUS       Exported                7813 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Mammalian expression of human ACE2.
ACCESSION   .
VERSION     .
KEYWORDS    hACE2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7813)
  AUTHORS   Li W, Moore MJ, Vasilieva N, Sui J, Wong SK, Berne MA, Somasundaran 
            M, Sullivan JL, Luzuriaga K, Greenough TC, Choe H, Farzan M
  TITLE     Angiotensin-converting enzyme 2 is a functional receptor for the 
            SARS coronavirus.
  JOURNAL   Nature. 2003 Nov 27. 426(6965):450-4.
  PUBMED    14647384
REFERENCE   2  (bases 1 to 7813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7813
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3172..3189)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3178..3402
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3448..3876
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3535..3554)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3745..3766
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3885..3905)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3890..4219
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4070..4205
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4132..4151
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(4340..4359)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4948..4967
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5252..5373
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5289..5308)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5343..5362
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5422..5438)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5422..5438)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5435..5457)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5446..5462
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5470..5500)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5515..5536
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5653..5670)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5824..6412)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5904..5923)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6583..7443)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7206..7225
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7444..7548)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7623..7642)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagcgttt aaacgggccc tctagaccat gtcaagctct tcctggctcc ttctcagcct
      721 tgttgctgta actgctgctc agtccaccat tgaggaacag gccaagacat ttttggacaa
      781 gtttaaccac gaagccgaag acctgttcta tcaaagttca cttgcttctt ggaattataa
      841 caccaatatt actgaagaga atgtccaaaa catgaataat gctggggaca aatggtctgc
      901 ctttttaaag gaacagtcca cacttgccca aatgtatcca ctacaagaaa ttcagaatct
      961 cacagtcaag cttcagctgc aggctcttca gcaaaatggg tcttcagtgc tctcagaaga
     1021 caagagcaaa cggttgaaca caattctaaa tacaatgagc accatctaca gtactggaaa
     1081 agtttgtaac ccagataatc cacaagaatg cttattactt gaaccaggtt tgaatgaaat
     1141 aatggcaaac agtttagact acaatgagag gctctgggct tgggaaagct ggagatctga
     1201 ggtcggcaag cagctgaggc cattatatga agagtatgtg gtcttgaaaa atgagatggc
     1261 aagagcaaat cattatgagg actatgggga ttattggaga ggagactatg aagtaaatgg
     1321 ggtagatggc tatgactaca gccgcggcca gttgattgaa gatgtggaac atacctttga
     1381 agagattaaa ccattatatg aacatcttca tgcctatgtg agggcaaagt tgatgaatgc
     1441 ctatccttcc tatatcagtc caattggatg cctccctgct catttgcttg gtgatatgtg
     1501 gggtagattt tggacaaatc tgtactcttt gacagttccc tttggacaga aaccaaacat
     1561 agatgttact gatgcaatgg tggaccaggc ctgggatgca cagagaatat tcaaggaggc
     1621 cgagaagttc tttgtatctg ttggtcttcc taatatgact caaggattct gggaaaattc
     1681 catgctaacg gacccaggaa atgttcagaa agcagtctgc catcccacag cttgggacct
     1741 ggggaagggc gacttcagga tccttatgtg cacaaaggtg acaatggacg acttcctgac
     1801 agctcatcat gagatggggc atatccagta tgatatggca tatgctgcac aaccttttct
     1861 gctaagaaat ggagctaatg aaggattcca tgaagctgtt ggggaaatca tgtcactttc
     1921 tgcagccaca cctaagcatt taaaatccat tggtcttctg tcacccgatt ttcaagaaga
     1981 caatgaaaca gaaataaact tcctgctcaa acaagcactc acgattgttg ggactctgcc
     2041 atttacttac atgttagaga agtggaggtg gatggtcttt aaaggggaaa ttcccaaaga
     2101 ccagtggatg aaaaagtggt gggagatgaa gcgagagata gttggggtgg tggaacctgt
     2161 gccccatgat gaaacatact gtgaccccgc atctctgttc catgtttcta atgattactc
     2221 attcattcga tattacacaa ggacccttta ccaattccag tttcaagaag cactttgtca
     2281 agcagctaaa catgaaggcc ctctgcacaa atgtgacatc tcaaactcta cagaagctgg
     2341 acagaaactg ttcaatatgc tgaggcttgg aaaatcagaa ccctggaccc tagcattgga
     2401 aaatgttgta ggagcaaaga acatgaatgt aaggccactg ctcaactact ttgagccctt
     2461 atttacctgg ctgaaagacc agaacaagaa ttcttttgtg ggatggagta ccgactggag
     2521 tccatatgca gaccaaagca tcaaagtgag gataagccta aaatcagctc ttggagataa
     2581 agcatatgaa tggaacgaca atgaaatgta cctgttccga tcatctgttg catatgctat
     2641 gaggcagtac tttttaaaag taaaaaatca gatgattctt tttggggagg aggatgtgcg
     2701 agtggctaat ttgaaaccaa gaatctcctt taatttcttt gtcactgcac ctaaaaatgt
     2761 gtctgatatc attcctagaa ctgaagttga aaaggccatc aggatgtccc ggagccgtat
     2821 caatgatgct ttccgtctga atgacaacag cctagagttt ctggggatac agccaacact
     2881 tggacctcct aaccagcccc ctgtttccat atggctgatt gtttttggag ttgtgatggg
     2941 agtgatagtg gttggcattg tcatcctgat cttcactggg atcagagatc ggaagaagaa
     3001 aaataaagca agaagtggag aaaatcctta tgcctccatc gatattagca aaggagaaaa
     3061 taatccagga ttccaaaaca ctgatgatgt tcagacctcc tttggtaccg agacctccca
     3121 ggtggcgccc gcttaagctt ggatccgcgt taagtttaaa ccgctgatca gcctcgactg
     3181 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     3241 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     3301 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     3361 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
     3421 ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg
     3481 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     3541 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     3601 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     3661 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     3721 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     3781 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     3841 aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt
     3901 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     3961 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     4021 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     4081 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     4141 agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg
     4201 aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca
     4261 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
     4321 ggccgcttgg gtggagaggc tattcggcta tgactgggca acagacaatc ggctgctctg
     4381 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
     4441 tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga
     4501 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
     4561 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
     4621 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
     4681 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
     4741 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
     4801 ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct
     4861 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
     4921 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
     4981 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
     5041 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat
     5101 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta
     5161 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     5221 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
     5281 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     5341 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
     5401 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     5461 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     5521 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     5581 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     5641 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     5701 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     5761 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     5821 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     5881 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     5941 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     6001 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     6061 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     6121 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     6181 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     6241 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     6301 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     6361 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     6421 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     6481 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     6541 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     6601 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     6661 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     6721 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     6781 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     6841 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     6901 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     6961 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     7021 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     7081 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     7141 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     7201 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     7261 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     7321 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     7381 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     7441 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     7501 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     7561 aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc
     7621 agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg
     7681 gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac
     7741 aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc
     7801 agatatacgc gtt
//
