LOCUS Exported 9984 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Encodes CRY2-mCherry-dPLD-P2A-CIBN-2xFYVE for mammalian expression. ACCESSION . VERSION . KEYWORDS Endosome-targeted optodPLD SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9984) AUTHORS Tei R, Baskin JM TITLE Spatiotemporal control of phosphatidic acid signaling with optogenetic, engineered phospholipase Ds. JOURNAL J Cell Biol. 2020 Mar 2;219(3). pii: 133675. doi: 10.1083/jcb.201907013. PUBMED 31999306 REFERENCE 2 (bases 1 to 9984) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9984 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 1..380 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 381..584 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 535..555 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" protein_bind 586..604 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" primer_bind 614..638 /label=LNCX /note="Human CMV promoter, forward primer" CDS 740..2233 /codon_start=1 /product="N-terminal photolyase homology region of Arabidopsis thaliana cryptochrome 2" /label=CRY2PHR /note="binds to CIB1 upon exposure to blue light (Kennedy et al., 2010)" /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWRKCLDMSI ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA" CDS 2267..2971 /codon_start=1 /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /label=mCherry /note="mammalian codon-optimized" /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE GRHSTGGMDELYK" primer_bind complement(2409..2427) /label=mCherry-R /note="mCherry, reverse primer" primer_bind 2677..2696 /label=mCherry-F /note="mCherry, forward primer" CDS 4586..4642 /codon_start=1 /product="2A peptide from porcine teschovirus-1 polyprotein" /label=P2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="ATNFSLLKQAGDVEENPGP" primer_bind complement(5702..5719) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 5708..5932 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" primer_bind complement(6065..6084) /label=F1ori-R /note="F1 origin, reverse primer" protein_bind 6216..6263 /label=FRT /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="FLP-mediated recombination occurs in the 8-bp core sequence TCTAGAAA (Turan and Bode, 2011)." CDS 6271..7293 /codon_start=1 /gene="aph(4)-Ia" /product="aminoglycoside phosphotransferase from E. coli" /label=HygR /note="confers resistance to hygromycin" /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE " polyA_signal 7423..7544 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(7460..7479) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 7514..7533 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(7593..7609) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(7593..7609) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(7606..7628) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 7617..7633 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(7641..7671) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 7686..7707 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(7824..7841) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(7995..8583) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(8075..8094) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(8754..9614) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 9377..9396 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(9615..9719) /gene="bla" /label=AmpR promoter primer_bind complement(9794..9813) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" ORIGIN 1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc 61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca 121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga 181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc 241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct 301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat 361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc 421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt 481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa 541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctccct atcagtgata 601 gagatcgtcg acgagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct 661 gttttgacct ccatagaaga caccgggacc gatccagcct ccggactcta gcgtttaaac 721 ttaagcttgg tacgccacca tgaagatgga caaaaagact atagtttggt ttagaagaga 781 cctaaggatt gaggataatc ctgcattagc agcagctgct cacgaaggat ctgtttttcc 841 tgtcttcatt tggtgtcctg aagaagaagg acagttttat cctggaagag cttcaagatg 901 gtggatgaaa caatcacttg ctcacttatc tcaatccttg aaggctcttg gatctgacct 961 cactttaatc aaaacccaca acacgatttc agcgatcttg gattgtatcc gcgttaccgg 1021 tgctacaaaa gtcgtcttta accacctcta tgatcctgtt tcgttagttc gggaccatac 1081 cgtaaaggag aagctggtgg aacgtgggat ctctgtgcaa agctacaatg gagatctatt 1141 gtatgaaccg tgggagatat actgcgaaaa gggcaaacct tttacgagtt tcaattctta 1201 ctggaggaaa tgcttagata tgtcgattga atccgttatg cttcctcctc cttggcggtt 1261 gatgccaata actgcagcgg ctgaagcgat ttgggcgtgt tcgattgaag aactagggct 1321 ggagaatgag gccgagaaac cgagcaatgc gttgttaact agagcttggt ctccaggatg 1381 gagcaatgct gataagttac taaatgagtt catcgagaag cagttgatag attatgcaaa 1441 gaacagcaag aaagttgttg ggaattctac ttcactactt tctccgtatc tccatttcgg 1501 ggaaataagc gtcagacacg ttttccagtg tgcccggatg aaacaaatta tatgggcaag 1561 agataagaac agtgaaggag aagaaagtgc agatcttttt cttaggggaa tcggtttaag 1621 agagtattct cggtatatat gtttcaactt cccgtttact cacgagcaat cgttgttgag 1681 tcatcttcgg tttttccctt gggatgctga tgttgataag ttcaaggcct ggagacaagg 1741 caggaccggt tatccgttgg tggatgccgg aatgagagag ctttgggcta ccggatggat 1801 gcataacaga ataagagtga ttgtttcaag ctttgctgtg aagtttcttc tccttccatg 1861 gaaatgggga atgaagtatt tctgggatac acttttggat gctgatttgg aatgtgacat 1921 ccttggctgg cagtatatct ctgggagtat ccccgatggc cacgagcttg atcgcttgga 1981 caatcccgcg ttacaaggcg ccaaatatga cccagaaggt gagtacataa ggcaatggct 2041 tcccgagctt gcgagattgc caactgaatg gatccatcat ccatgggacg ctcctttaac 2101 cgtactcaaa gcttctggtg tggaactcgg aacaaactat gcgaaaccca ttgtagacat 2161 cgacacagct cgtgagctac tagctaaagc tatttcaaga acccgtgaag cacagatcat 2221 gatcggagca gcaggtggag gcggttcagg cggaggtggc agcggcgtga gcaagggcga 2281 ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc 2341 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac 2401 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct 2461 gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga 2521 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga 2581 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa 2641 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat 2701 gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat 2761 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta 2821 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat 2881 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca 2941 ctccaccggc ggcatggacg agctgtacaa gagatctcga agcgcggccg cgggagcagg 3001 aggagcagct cgaggcgcag cagcgggagc aggaggagca ggtcgatcgg gcggatctgc 3061 agattcagct acaccacatt tggacgccgt tgagcaaaca ttgaggcaag tgtccccagg 3121 cctggagggc gatgtatggg aaagaactag tggaaataaa ttggacggaa gtgccgctga 3181 tcccagtgat tggttgttgc aaaccccagg atgttggggc gatgataaat gcgctgacag 3241 agttgggaca aaaaggttgc tggctaagat gactgaaaat atagggaatg ccaccagaac 3301 cgtagatatt agtacacttg cacctttccc aaacggcgca ttccaagacg ctattgtcgc 3361 tggtttgaag gagtccgcag ctaaaggcaa taagttgaaa gttaggatac tggttggggc 3421 tgcacccgtc taccacatga atgtaatacc ttccaagtat agggacgaat tgactgcaaa 3481 acttggcaaa gccgcagaaa acataacttt gaacgtggct agtatgacta catcaaaaac 3541 agctttctcc tggaacgcta gcaaaatctt ggttgtagac gggcaaagtg cccttactgg 3601 tggaataaat agctggaagg atgattactt ggatactact catccagtgt ctgatgttga 3661 tcttgcactg accggaccag ctgcaggctc tgccggtaga taccttgaca ctttgtggac 3721 atggacctgc caaaacaaga gtaacatcgc ttccgtctgg tttgcagctt ccggcaacgc 3781 agggtgcatg cccactatgc ataaagatac aaaccccaag gcatcacctg ccaccgggaa 3841 tgtcccagta atagccgttg gagggttggg cgttggcatc aaagatgtgg accccaagtc 3901 cactttcagg cccgatctgc caacagctag cgatactaag tgtgtggtag gacttcacga 3961 caatactaac gccgatagag actatgatac agtcaaccca gaagagagtg ccttgagagc 4021 tcttgttgct tcagcaaaag gtcacattga aatatctcag caggatttga acgctacatg 4081 tccacccttg cctaggtatg acattagatt gtacgatgcc ttggctgcta aaatggctgc 4141 tggggttaag gtgagaattg tggttagcga cccagccaac aggggcgcag tgggcagtgg 4201 gggttacagc caaataaaat cacttagtga aatatcagac actttgagaa acaggcttgc 4261 aaatatcaca ggcggtcaac aagctgccaa aactgcaatg tgtagcaatt tgcagcttgc 4321 aacctttaga tcctccccca acggcaaatg ggcagacggg catccttatg cccaacacca 4381 taagttggtt tccgtcgact catccacatt ttatatcggt tcaaaaaatt tgtacccatc 4441 ctggctgcaa gattttggct acatagtgga atccccagag gctgccaagc aactggatgc 4501 caaattgctt gaccctcagt ggaagtactc acaggagact gcaacagtgg attacgcaag 4561 aggaatctgc aacgctggaa gcggcgctac aaactttagt ctgctcaagc aggctggcga 4621 tgtcgaggaa aacccaggtc ccggtaccat gaatggagct ataggaggtg accttttgct 4681 caattttcct gacatgtcgg tcctagagcg ccaaagggct cacctcaagt acctcaatcc 4741 cacctttgat tctcctctcg ccggcttctt tgccgattct tcaatgatta ccggcggcga 4801 gatggacagc tatctttcga ctgccggttt gaatcttccg atgatgtacg gtgagacgac 4861 ggtggaaggt gattcaagac tctcaatttc gccggaaacg acgcttggga ctggaaattt 4921 caaggcagcg aagtttgata cagagactaa ggattgtaat gaggcggcga agaagatgac 4981 gatgaacaga gatgacctag tagaagaagg agaagaagag aagtcgaaaa taacagagca 5041 aaacaatggg agcacaaaaa gcatcaagaa gatgaaacac aaagccaaga aagaagagaa 5101 caatttctct aatgattcat ctaaagtgac gaaggaattg gagaaaacgg attatattca 5161 tgtgaccggt ggcgccttag aattcgaaag tgatgccatg ttcgctgctg aaagagcccc 5221 tgactgggtg gatgctgagg aatgccatcg gtgcagagta cagtttgggg tggtgacccg 5281 caagcatcac tgccgagcat gtgggcagat cttctgtggc aagtgctcct ccaagtactc 5341 caccatcccc aagttcggca ttgagaagga ggtgcgcgtg tgtgagccct gctatgagca 5401 gctgaacaag aaggcacaag gacaaggatc cgaaagtgat gccatgttcg ctgctgaaag 5461 agcccctgac tgggtggatg ctgaggaatg ccatcggtgc agagtacagt ttggggtggt 5521 gacccgcaag catcactgcc gagcatgtgg gcagatcttc tgtggcaagt gctcctccaa 5581 gtactccacc atccccaagt tcggcattga gaaggaggtg cgcgtgtgtg agccctgcta 5641 tgagcagctg aacaagaagg catgatctag agggccctat tcgtttaaac ccgctgatca 5701 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc 5761 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg 5821 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg 5881 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag 5941 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta 6001 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg 6061 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa 6121 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc 6181 aaaaaacttg attagggtga tggttcacgt acctagaagt tcctattccg aagttcctat 6241 tctctagaaa gtataggaac ttccttggcc aaaaagcctg aactcaccgc gacgtctgtc 6301 gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct ctcggagggc 6361 gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat 6421 agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc atcggccgcg 6481 ctcccgattc cggaagtgct tgacattggg gaattcagcg agagcctgac ctattgcatc 6541 tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt 6601 ctgcagccgg tcgcggaggc catggatgcg atcgctgcgg ccgatcttag ccagacgagc 6661 gggttcggcc cattcggacc gcaaggaatc ggtcaataca ctacatggcg tgatttcata 6721 tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg tgatggacga caccgtcagt 6781 gcgtccgtcg cgcaggctct cgatgagctg atgctttggg ccgaggactg ccccgaagtc 6841 cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa tggccgcata 6901 acagcggtca ttgactggag cgaggcgatg ttcggggatt cccaatacga ggtcgccaac 6961 atcttcttct ggaggccgtg gttggcttgt atggagcagc agacgcgcta cttcgagcgg 7021 aggcatccgg agcttgcagg atcgccgcgg ctccgggcgt atatgctccg cattggtctt 7081 gaccaactct atcagagctt ggttgacggc aatttcgatg atgcagcttg ggcgcagggt 7141 cgatgcgacg caatcgtccg atccggagcc gggactgtcg ggcgtacaca aatcgcccgc 7201 agaagcgcgg ccgtctggac cgatggctgt gtagaagtac tcgccgatag tggaaaccga 7261 cgccccagca ctcgtccgag ggcaaaggaa tagcacgtac tacgagattt cgattccacc 7321 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc 7381 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct 7441 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca 7501 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg 7561 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt 7621 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt 7681 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg 7741 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg 7801 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg 7861 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat 7921 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc 7981 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc 8041 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga 8101 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt 8161 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg 8221 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc 8281 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg 8341 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc 8401 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg 8461 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc 8521 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct 8581 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt 8641 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa 8701 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa 8761 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc 8821 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct 8881 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca 8941 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt 9001 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt 9061 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc 9121 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc 9181 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt 9241 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact 9301 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc 9361 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt 9421 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg 9481 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct 9541 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa 9601 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt 9661 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc 9721 acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat 9781 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg 9841 cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag 9901 gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg 9961 atgtacgggc cagatatacg cgtt //