LOCUS       Exported                9610 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Encodes CRY2-mCherry-dPLD-P2A-CIBN-Sialyltransferase(TM) for 
            mammalian expression.
ACCESSION   .
VERSION     .
KEYWORDS    Golgi-targeted optodPLD
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9610)
  AUTHORS   Tei R, Baskin JM
  TITLE     Spatiotemporal control of phosphatidic acid signaling with 
            optogenetic, engineered phospholipase Ds.
  JOURNAL   J Cell Biol. 2020 Mar 2;219(3). pii: 133675. doi: 
            10.1083/jcb.201907013.
  PUBMED    31999306
REFERENCE   2  (bases 1 to 9610)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9610
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    586..604
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    607..625
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     635..659
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             761..2254
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWRKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     CDS             2288..2992
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(2430..2448)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2698..2717
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             4607..4663
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(5328..5345)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5334..5558
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5691..5710)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    5842..5889
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5897..6919
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELSAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    7049..7170
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7086..7105)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7140..7159
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7219..7235)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7219..7235)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7232..7254)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7243..7259
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7267..7297)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7312..7333
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7450..7467)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7621..8209)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7701..7720)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8380..9240)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9003..9022
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9241..9345)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9420..9439)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctccct atcagtgata
      601 gagatctccc tatcagtgat agagatcgtc gacgagctcg tttagtgaac cgtcagatcg
      661 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc
      721 tccggactct agcgtttaaa cttaagcttg gtacgccacc atgaagatgg acaaaaagac
      781 tatagtttgg tttagaagag acctaaggat tgaggataat cctgcattag cagcagctgc
      841 tcacgaagga tctgtttttc ctgtcttcat ttggtgtcct gaagaagaag gacagtttta
      901 tcctggaaga gcttcaagat ggtggatgaa acaatcactt gctcacttat ctcaatcctt
      961 gaaggctctt ggatctgacc tcactttaat caaaacccac aacacgattt cagcgatctt
     1021 ggattgtatc cgcgttaccg gtgctacaaa agtcgtcttt aaccacctct atgatcctgt
     1081 ttcgttagtt cgggaccata ccgtaaagga gaagctggtg gaacgtggga tctctgtgca
     1141 aagctacaat ggagatctat tgtatgaacc gtgggagata tactgcgaaa agggcaaacc
     1201 ttttacgagt ttcaattctt actggaggaa atgcttagat atgtcgattg aatccgttat
     1261 gcttcctcct ccttggcggt tgatgccaat aactgcagcg gctgaagcga tttgggcgtg
     1321 ttcgattgaa gaactagggc tggagaatga ggccgagaaa ccgagcaatg cgttgttaac
     1381 tagagcttgg tctccaggat ggagcaatgc tgataagtta ctaaatgagt tcatcgagaa
     1441 gcagttgata gattatgcaa agaacagcaa gaaagttgtt gggaattcta cttcactact
     1501 ttctccgtat ctccatttcg gggaaataag cgtcagacac gttttccagt gtgcccggat
     1561 gaaacaaatt atatgggcaa gagataagaa cagtgaagga gaagaaagtg cagatctttt
     1621 tcttagggga atcggtttaa gagagtattc tcggtatata tgtttcaact tcccgtttac
     1681 tcacgagcaa tcgttgttga gtcatcttcg gtttttccct tgggatgctg atgttgataa
     1741 gttcaaggcc tggagacaag gcaggaccgg ttatccgttg gtggatgccg gaatgagaga
     1801 gctttgggct accggatgga tgcataacag aataagagtg attgtttcaa gctttgctgt
     1861 gaagtttctt ctccttccat ggaaatgggg aatgaagtat ttctgggata cacttttgga
     1921 tgctgatttg gaatgtgaca tccttggctg gcagtatatc tctgggagta tccccgatgg
     1981 ccacgagctt gatcgcttgg acaatcccgc gttacaaggc gccaaatatg acccagaagg
     2041 tgagtacata aggcaatggc ttcccgagct tgcgagattg ccaactgaat ggatccatca
     2101 tccatgggac gctcctttaa ccgtactcaa agcttctggt gtggaactcg gaacaaacta
     2161 tgcgaaaccc attgtagaca tcgacacagc tcgtgagcta ctagctaaag ctatttcaag
     2221 aacccgtgaa gcacagatca tgatcggagc agcaggtgga ggcggttcag gcggaggtgg
     2281 cagcggcgtg agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt
     2341 caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga
     2401 gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct
     2461 gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa
     2521 gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga
     2581 gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca
     2641 ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc
     2701 cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga
     2761 cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga
     2821 cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa
     2881 cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta
     2941 cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca agagatctcg
     3001 aagcgcggcc gcgggagcag gaggagcagc tcgaggcgca gcagcgggag caggaggagc
     3061 aggtcgatcg ggcggatctg cagattcagc tacaccacat ttggacgccg ttgagcaaac
     3121 attgaggcaa gtgtccccag gcctggaggg cgatgtatgg gaaagaacta gtggaaataa
     3181 attggacgga agtgccgctg atcccagtga ttggttgttg caaaccccag gatgttgggg
     3241 cgatgataaa tgcgctgaca gagttgggac aaaaaggttg ctggctaaga tgactgaaaa
     3301 tatagggaat gccaccagaa ccgtagatat tagtacactt gcacctttcc caaacggcgc
     3361 attccaagac gctattgtcg ctggtttgaa ggagtccgca gctaaaggca ataagttgaa
     3421 agttaggata ctggttgggg ctgcacccgt ctaccacatg aatgtaatac cttccaagta
     3481 tagggacgaa ttgactgcaa aacttggcaa agccgcagaa aacataactt tgaacgtggc
     3541 tagtatgact acatcaaaaa cagctttctc ctggaacgct agcaaaatct tggttgtaga
     3601 cgggcaaagt gcccttactg gtggaataaa tagctggaag gatgattact tggatactac
     3661 tcatccagtg tctgatgttg atcttgcact gaccggacca gctgcaggct ctgccggtag
     3721 ataccttgac actttgtgga catggacctg ccaaaacaag agtaacatcg cttccgtctg
     3781 gtttgcagct tccggcaacg cagggtgcat gcccactatg cataaagata caaaccccaa
     3841 ggcatcacct gccaccggga atgtcccagt aatagccgtt ggagggttgg gcgttggcat
     3901 caaagatgtg gaccccaagt ccactttcag gcccgatctg ccaacagcta gcgatactaa
     3961 gtgtgtggta ggacttcacg acaatactaa cgccgataga gactatgata cagtcaaccc
     4021 agaagagagt gccttgagag ctcttgttgc ttcagcaaaa ggtcacattg aaatatctca
     4081 gcaggatttg aacgctacat gtccaccctt gcctaggtat gacattagat tgtacgatgc
     4141 cttggctgct aaaatggctg ctggggttaa ggtgagaatt gtggttagcg acccagccaa
     4201 caggggcgca gtgggcagtg ggggttacag ccaaataaaa tcacttagtg aaatatcaga
     4261 cactttgaga aacaggcttg caaatatcac aggcggtcaa caagctgcca aaactgcaat
     4321 gtgtagcaat ttgcagcttg caacctttag atcctccccc aacggcaaat gggcagacgg
     4381 gcatccttat gcccaacacc ataagttggt ttccgtcgac tcatccacat tttatatcgg
     4441 ttcaaaaaat ttgtacccat cctggctgca agattttggc tacatagtgg aatccccaga
     4501 ggctgccaag caactggatg ccaaattgct tgaccctcag tggaagtact cacaggagac
     4561 tgcaacagtg gattacgcaa gaggaatctg caacgctgga agcggcgcta caaactttag
     4621 tctgctcaag caggctggcg atgtcgagga aaacccaggt cccggtacca tgaatggagc
     4681 tataggaggt gaccttttgc tcaattttcc tgacatgtcg gtcctagagc gccaaagggc
     4741 tcacctcaag tacctcaatc ccacctttga ttctcctctc gccggcttct ttgccgattc
     4801 ttcaatgatt accggcggcg agatggacag ctatctttcg actgccggtt tgaatcttcc
     4861 gatgatgtac ggtgagacga cggtggaagg tgattcaaga ctctcaattt cgccggaaac
     4921 gacgcttggg actggaaatt tcaaggcagc gaagtttgat acagagacta aggattgtaa
     4981 tgaggcggcg aagaagatga cgatgaacag agatgaccta gtagaagaag gagaagaaga
     5041 gaagtcgaaa ataacagagc aaaacaatgg gagcacaaaa agcatcaaga agatgaaaca
     5101 caaagccaag aaagaagaga acaatttctc taatgattca tctaaagtga cgaaggaatt
     5161 ggagaaaacg gattatattc atgtgaccgg tatacatact aatctgaaaa agaagttcag
     5221 cctcttcatc ctggtcttcc tgctcttcgc tgtgatctgc gtttggaaga agggctctga
     5281 ttacgaggcc cttacactgt aagggcccgt ttaaacccgc tgatcagcct cgactgtgcc
     5341 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     5401 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     5461 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     5521 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
     5581 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     5641 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     5701 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     5761 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     5821 gggtgatggt tcacgtacct agaagttcct attccgaagt tcctattctc tagaaagtat
     5881 aggaacttcc ttggccaaaa agcctgaact caccgcgacg tctgtcgaga agtttctgat
     5941 cgaaaagttc gacagcgtct ccgacctgat gcagctctcg gagggcgaag aatctcgtgc
     6001 tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg
     6061 tttctacaaa gatcgttatg tttatcggca ctttgcatcg gccgcgctcc cgattccgga
     6121 agtgcttgac attggggaat tcagcgagag cctgacctat tgcatctccc gccgtgcaca
     6181 gggtgtcacg ttgcaagacc tgcctgaaac cgaactgtcc gctgttctgc agccggtcgc
     6241 ggaggccatg gatgcgatcg ctgcggccga tcttagccag acgagcgggt tcggcccatt
     6301 cggaccgcaa ggaatcggtc aatacactac atggcgtgat ttcatatgcg cgattgctga
     6361 tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca
     6421 ggctctcgat gagctgatgc tttgggccga ggactgcccc gaagtccggc acctcgtgca
     6481 cgcggatttc ggctccaaca atgtcctgac ggacaatggc cgcataacag cggtcattga
     6541 ctggagcgag gcgatgttcg gggattccca atacgaggtc gccaacatct tcttctggag
     6601 gccgtggttg gcttgtatgg agcagcagac gcgctacttc gagcggaggc atccggagct
     6661 tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc aactctatca
     6721 gagcttggtt gacggcaatt tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat
     6781 cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt
     6841 ctggaccgat ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc ccagcactcg
     6901 tccgagggca aaggaatagc acgtactacg agatttcgat tccaccgccg ccttctatga
     6961 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     7021 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa
     7081 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     7141 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta
     7201 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     7261 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     7321 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     7381 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     7441 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     7501 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     7561 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     7621 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     7681 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     7741 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     7801 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     7861 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     7921 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     7981 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     8041 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     8101 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     8161 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     8221 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     8281 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     8341 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     8401 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     8461 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     8521 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     8581 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     8641 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     8701 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     8761 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     8821 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     8881 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     8941 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     9001 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     9061 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     9121 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     9181 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     9241 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     9301 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     9361 agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt
     9421 acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag
     9481 gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat
     9541 tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga
     9601 tatacgcgtt
//
