LOCUS Exported 9609 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Encodes CRY2-mCherry-PLD-P2A-CIBN-Sialyltransferase(TM) for mammalian expression. ACCESSION . VERSION . KEYWORDS Golgi-targeted optoPLD SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9609) AUTHORS Tei R, Baskin JM TITLE Spatiotemporal control of phosphatidic acid signaling with optogenetic, engineered phospholipase Ds. JOURNAL J Cell Biol. 2020 Mar 2;219(3). pii: 133675. doi: 10.1083/jcb.201907013. PUBMED 31999306 REFERENCE 2 (bases 1 to 9609) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9609 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 1..380 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 381..584 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 535..555 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" protein_bind 586..604 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 607..625 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" primer_bind 635..659 /label=LNCX /note="Human CMV promoter, forward primer" CDS 761..2254 /codon_start=1 /product="N-terminal photolyase homology region of Arabidopsis thaliana cryptochrome 2" /label=CRY2PHR /note="binds to CIB1 upon exposure to blue light (Kennedy et al., 2010)" /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWRKCLDMSI ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA" CDS 2288..2992 /codon_start=1 /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /label=mCherry /note="mammalian codon-optimized" /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE GRHSTGGMDELYK" primer_bind complement(2430..2448) /label=mCherry-R /note="mCherry, reverse primer" primer_bind 2698..2717 /label=mCherry-F /note="mCherry, forward primer" CDS 4607..4663 /codon_start=1 /product="2A peptide from porcine teschovirus-1 polyprotein" /label=P2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="ATNFSLLKQAGDVEENPGP" primer_bind complement(5327..5344) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 5333..5557 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" primer_bind complement(5690..5709) /label=F1ori-R /note="F1 origin, reverse primer" protein_bind 5841..5888 /label=FRT /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="FLP-mediated recombination occurs in the 8-bp core sequence TCTAGAAA (Turan and Bode, 2011)." CDS 5896..6918 /codon_start=1 /gene="aph(4)-Ia" /product="aminoglycoside phosphotransferase from E. coli" /label=HygR /note="confers resistance to hygromycin" /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE TELSAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE " polyA_signal 7048..7169 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(7085..7104) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 7139..7158 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(7218..7234) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(7218..7234) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(7231..7253) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 7242..7258 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(7266..7296) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 7311..7332 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(7449..7466) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(7620..8208) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(7700..7719) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(8379..9239) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 9002..9021 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(9240..9344) /gene="bla" /label=AmpR promoter primer_bind complement(9419..9438) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" ORIGIN 1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc 61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca 121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga 181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc 241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct 301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat 361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc 421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt 481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa 541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctccct atcagtgata 601 gagatctccc tatcagtgat agagatcgtc gacgagctcg tttagtgaac cgtcagatcg 661 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc 721 tccggactct agcgtttaaa cttaagcttg gtacgccacc atgaagatgg acaaaaagac 781 tatagtttgg tttagaagag acctaaggat tgaggataat cctgcattag cagcagctgc 841 tcacgaagga tctgtttttc ctgtcttcat ttggtgtcct gaagaagaag gacagtttta 901 tcctggaaga gcttcaagat ggtggatgaa acaatcactt gctcacttat ctcaatcctt 961 gaaggctctt ggatctgacc tcactttaat caaaacccac aacacgattt cagcgatctt 1021 ggattgtatc cgcgttaccg gtgctacaaa agtcgtcttt aaccacctct atgatcctgt 1081 ttcgttagtt cgggaccata ccgtaaagga gaagctggtg gaacgtggga tctctgtgca 1141 aagctacaat ggagatctat tgtatgaacc gtgggagata tactgcgaaa agggcaaacc 1201 ttttacgagt ttcaattctt actggaggaa atgcttagat atgtcgattg aatccgttat 1261 gcttcctcct ccttggcggt tgatgccaat aactgcagcg gctgaagcga tttgggcgtg 1321 ttcgattgaa gaactagggc tggagaatga ggccgagaaa ccgagcaatg cgttgttaac 1381 tagagcttgg tctccaggat ggagcaatgc tgataagtta ctaaatgagt tcatcgagaa 1441 gcagttgata gattatgcaa agaacagcaa gaaagttgtt gggaattcta cttcactact 1501 ttctccgtat ctccatttcg gggaaataag cgtcagacac gttttccagt gtgcccggat 1561 gaaacaaatt atatgggcaa gagataagaa cagtgaagga gaagaaagtg cagatctttt 1621 tcttagggga atcggtttaa gagagtattc tcggtatata tgtttcaact tcccgtttac 1681 tcacgagcaa tcgttgttga gtcatcttcg gtttttccct tgggatgctg atgttgataa 1741 gttcaaggcc tggagacaag gcaggaccgg ttatccgttg gtggatgccg gaatgagaga 1801 gctttgggct accggatgga tgcataacag aataagagtg attgtttcaa gctttgctgt 1861 gaagtttctt ctccttccat ggaaatgggg aatgaagtat ttctgggata cacttttgga 1921 tgctgatttg gaatgtgaca tccttggctg gcagtatatc tctgggagta tccccgatgg 1981 ccacgagctt gatcgcttgg acaatcccgc gttacaaggc gccaaatatg acccagaagg 2041 tgagtacata aggcaatggc ttcccgagct tgcgagattg ccaactgaat ggatccatca 2101 tccatgggac gctcctttaa ccgtactcaa agcttctggt gtggaactcg gaacaaacta 2161 tgcgaaaccc attgtagaca tcgacacagc tcgtgagcta ctagctaaag ctatttcaag 2221 aacccgtgaa gcacagatca tgatcggagc agcaggtgga ggcggttcag gcggaggtgg 2281 cagcggcgtg agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt 2341 caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga 2401 gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct 2461 gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa 2521 gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga 2581 gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca 2641 ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc 2701 cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga 2761 cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga 2821 cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa 2881 cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta 2941 cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca agagatctcg 3001 aagcgcggcc gcgggagcag gaggagcagc tcgaggcgca gcagcgggag caggaggagc 3061 aggtcgatcg ggcggatctg cagattcagc tacaccacat ttggacgccg ttgagcaaac 3121 attgaggcaa gtgtccccag gcctggaggg cgatgtatgg gaaagaacta gtggaaataa 3181 attggacgga agtgccgctg atcccagtga ttggttgttg caaaccccag gatgttgggg 3241 cgatgataaa tgcgctgaca gagttgggac aaaaaggttg ctggctaaga tgactgaaaa 3301 tatagggaat gccaccagaa ccgtagatat tagtacactt gcacctttcc caaacggcgc 3361 attccaagac gctattgtcg ctggtttgaa ggagtccgca gctaaaggca ataagttgaa 3421 agttaggata ctggttgggg ctgcacccgt ctaccacatg aatgtaatac cttccaagta 3481 tagggacgaa ttgactgcaa aacttggcaa agccgcagaa aacataactt tgaacgtggc 3541 tagtatgact acatcaaaaa cagctttctc ctggaaccat agcaaaatct tggttgtaga 3601 cgggcaaagt gcccttactg gtggaataaa tagctggaag gatgattact tggatactac 3661 tcatccagtg tctgatgttg atcttgcact gaccggacca gctgcaggct ctgccggtag 3721 ataccttgac actttgtgga catggacctg ccaaaacaag agtaacatcg cttccgtctg 3781 gtttgcagct tccggcaacg cagggtgcat gcccactatg cataaagata caaaccccaa 3841 ggcatcacct gccaccggga atgtcccagt aatagccgtt ggagggttgg gcgttggcat 3901 caaagatgtg gaccccaagt ccactttcag gcccgatctg ccaacagcta gcgatactaa 3961 gtgtgtggta ggacttcacg acaatactaa cgccgataga gactatgata cagtcaaccc 4021 agaagagagt gccttgagag ctcttgttgc ttcagcaaaa ggtcacattg aaatatctca 4081 gcaggatttg aacgctacat gtccaccctt gcctaggtat gacattagat tgtacgatgc 4141 cttggctgct aaaatggctg ctggggttaa ggtgagaatt gtggttagcg acccagccaa 4201 caggggcgca gtgggcagtg ggggttacag ccaaataaaa tcacttagtg aaatatcaga 4261 cactttgaga aacaggcttg caaatatcac aggcggtcaa caagctgcca aaactgcaat 4321 gtgtagcaat ttgcagcttg caacctttag atcctccccc aacggcaaat gggcagacgg 4381 gcatccttat gcccaacacc ataagttggt ttccgtcgac tcatccacat tttatatcgg 4441 ttcaaaaaat ttgtacccat cctggctgca agattttggc tacatagtgg aatccccaga 4501 ggctgccaag caactggatg ccaaattgct tgaccctcag tggaagtact cacaggagac 4561 tgcaacagtg gattacgcaa gaggaatctg caacgctgga agcggcgcta caaactttag 4621 tctgctcaag caggctggcg atgtcgagga aaacccaggt cccggtacca tgaatggagc 4681 tataggaggt gaccttttgc tcaattttcc tgacatgtcg gtcctagagc gccaaagggc 4741 tcacctcaag tacctcaatc ccacctttga ttctcctctc gccggcttct ttgccgattc 4801 ttcaatgatt accggcggcg agatggacag ctatctttcg actgccggtt tgaatcttcc 4861 gatgatgtac ggtgagacga cggtggaagg tgattcaaga ctctcaattt cgccggaaac 4921 gacgcttggg actggaaatt tcaaggcagc gaagtttgat acagagacta aggattgtaa 4981 tgaggcggcg aagaagatga cgatgaacag agatgaccta gtagaagaag gagaagaaga 5041 gaagtcgaaa ataacagagc aaaacaatgg gagcacaaaa agcatcaaga agatgaaaca 5101 caaagccaag aaagaagaga acaatttctc taatgattca tctaaagtga cgaaggaatt 5161 ggagaaaacg gattatattc atgtgaccgg tatacatact aatctgaaaa agaagttcag 5221 cctcttcatc ctggtcttcc tgctcttcgc tgtgatctgc gtttggaaga agggctctga 5281 ttacgaggcc cttacactgt aaggcccgtt taaacccgct gatcagcctc gactgtgcct 5341 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt 5401 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg 5461 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac 5521 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc 5581 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg 5641 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct 5701 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg 5761 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag 5821 ggtgatggtt cacgtaccta gaagttccta ttccgaagtt cctattctct agaaagtata 5881 ggaacttcct tggccaaaaa gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc 5941 gaaaagttcg acagcgtctc cgacctgatg cagctctcgg agggcgaaga atctcgtgct 6001 ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg taaatagctg cgccgatggt 6061 ttctacaaag atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc gattccggaa 6121 gtgcttgaca ttggggaatt cagcgagagc ctgacctatt gcatctcccg ccgtgcacag 6181 ggtgtcacgt tgcaagacct gcctgaaacc gaactgtccg ctgttctgca gccggtcgcg 6241 gaggccatgg atgcgatcgc tgcggccgat cttagccaga cgagcgggtt cggcccattc 6301 ggaccgcaag gaatcggtca atacactaca tggcgtgatt tcatatgcgc gattgctgat 6361 ccccatgtgt atcactggca aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag 6421 gctctcgatg agctgatgct ttgggccgag gactgccccg aagtccggca cctcgtgcac 6481 gcggatttcg gctccaacaa tgtcctgacg gacaatggcc gcataacagc ggtcattgac 6541 tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt cttctggagg 6601 ccgtggttgg cttgtatgga gcagcagacg cgctacttcg agcggaggca tccggagctt 6661 gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg gtcttgacca actctatcag 6721 agcttggttg acggcaattt cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc 6781 gtccgatccg gagccgggac tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc 6841 tggaccgatg gctgtgtaga agtactcgcc gatagtggaa accgacgccc cagcactcgt 6901 ccgagggcaa aggaatagca cgtactacga gatttcgatt ccaccgccgc cttctatgaa 6961 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat 7021 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa 7081 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt 7141 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag 7201 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt 7261 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc 7321 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc 7381 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct 7441 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca 7501 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac 7561 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt 7621 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg 7681 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 7741 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc 7801 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc 7861 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac 7921 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt 7981 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 8041 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc 8101 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt 8161 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg 8221 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc 8281 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa 8341 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag 8401 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg 8461 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga 8521 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag 8581 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa 8641 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc 8701 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca 8761 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg 8821 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat 8881 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc 8941 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg 9001 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg 9061 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt 9121 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca 9181 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata 9241 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac 9301 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa 9361 gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc actctcagta 9421 caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg 9481 tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt 9541 gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat 9601 atacgcgtt //