LOCUS       Exported                9983 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Tissue-specific knock-out in zebrafish, Cas9 and Cre driven by a UAS
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    pUAS:Cas9T2ACre;U6:sgRNA1;U6:sgRNA2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9983)
  AUTHORS   Di Donato V, De Santis F, Auer TO, Testa N, Sanchez-Iranzo H, 
            Mercader N, Concordet JP, Del Bene F
  TITLE     2C-Cas9: a versatile tool for clonal analysis of gene function.
  JOURNAL   Genome Res. 2016 Mar 8.
  PUBMED    26957310
REFERENCE   2  (bases 1 to 9983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9983
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     179..198
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(417..521)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(599..1054)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(736..757)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     948..967
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1180..1202
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1194..1211
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1195..1211
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1218..1237
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1218..1236
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1895..1989
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        2008..2246
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     2193..2214
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     regulatory      2261..2270
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             2279..2299
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2306..6406
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes MGAS15252 Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDDYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFGSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLADSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQIYNQLFEENPINASRVDAKAILSARLSKSRRL
                     ENLIAQLPGEKRNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNSEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGAYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDRGMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGHSLHEQIANLAGSPAIKKGILQTVKIVDELVKVMGH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFIKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             6319..6327
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             6422..6442
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6458..6511
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6512..7543
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MANLLTVHQNLPALPVDATSDEVRKNLMDMFRDHQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     promoter        complement(7551..7568)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7597..7616)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7651..7670
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(8342..8360)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8343..8360)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(8378..8394)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8378..8394)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8391..8413)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8402..8418
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8426..8456)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8471..8492
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8609..8626)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8780..9368)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8860..8879)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9539..416)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
       61 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
      121 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
      181 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
      241 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
      301 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
      361 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
      421 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
      481 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
      541 ccacctgatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggaa
      601 attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt
      661 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata
      721 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac
      781 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa
      841 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
      901 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
      961 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
     1021 cccgccgcgc ttaatgcgcc gctacagggc gcgtccattc gccattcagg ctgcgcaact
     1081 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat
     1141 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
     1201 cgacggccag tgaattgtaa tacgactcac tatagggcga attgggccca gaggtgtaaa
     1261 gtacttgagt aattttactt gattactgta cttaagtatt atttttgggg atttttactt
     1321 tacttgagta caattaaaaa tcaatacttt tacttttact taattacatt tttttagaaa
     1381 aaaaagtact ttttactcct tacaatttta tttacagtca aaaagtactt attttttgga
     1441 gatcacttca ttctattttc ccttgctatt accaaaccaa ttgaattgcg ctgatgccca
     1501 gtttaattta aatagatccc atgggtcgac tacgcttaca gctccaaaac tctacggtaa
     1561 acctacataa actgctggtt ttcaaatttt aaagaattta agggtttaca ggtttactac
     1621 tacacagtga tttactgaca catgtaagtg taaatgagtt gaataagtaa gtaagccata
     1681 taccacacat gaaacacata cccagaagtc actggtatat atagccgtcc tccagactcc
     1741 cagctcgaga gaccgagaga gggtctcagt ttaagagcta tgctggaaac agcatagcaa
     1801 gtttaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt
     1861 tggtcgacgg atccaagctt gcatgcctgc aggtcggagt actgtcctcc gagcggagta
     1921 ctgtcctccg agcggagtac tgtcctccga gcggagtact gtcctccgag cggagtactg
     1981 tcctccgagc ggagactcta gcgagcgccg gagtataaat agaggcgctt cgtctacgga
     2041 gcgacaattc aattcaaaca agcaaagtga acacgtcgct aagcgaaagc taagcaaata
     2101 aacaagcgca gctgaacaag ctaaacaatc tgcagtaaag tgcaagttaa agtgaatcaa
     2161 ttaaaagtaa ccagcaacca agtaaatcaa ctgcaactac tgaaatctgc caagaagtaa
     2221 ttattgaata caagaagaga actctgaata gggaattggg gccaccatgg cttctccacc
     2281 taagaagaag agaaaggtgg gaagcatgga taagaagtat agcatcggcc tggatattgg
     2341 aactaactcc gtgggttggg cagtgattac agacgactac aaggtcccta gcaagaaatt
     2401 taaggtgctg ggtaacaccg acaggcacag catcaagaaa aatctgattg gagccctgct
     2461 gttcggttct ggagagactg ccgaagcaac acgcctgaaa agaacagcaa gaaggcgcta
     2521 taccagaagg aagaatagaa tctgttacct gcaggagatt ttctctaacg aaatggctaa
     2581 ggtggacgat tcattctttc ataggctgga ggaaagtttc ctggtcgagg aagataagaa
     2641 acacgagcgc catcctatct ttggaaacat tgtggacgag gtcgcctatc acgaaaaata
     2701 cccaaccatc tatcatctgc gcaagaaact ggctgactct actgataaag ccgacctgag
     2761 actgatctat ctggctctgg cccacatgat taagttcagg ggtcattttc tgatcgaggg
     2821 cgatctgaac cccgacaatt ccgatgtgga caagctgttc atccagctgg tccagattta
     2881 caatcagctg tttgaggaaa accctattaa tgcttccaga gtggacgcaa aagctatcct
     2941 gtcagccagg ctgtccaagt cacgcagact ggagaacctg attgcacagc tgcccggaga
     3001 aaagaggaac ggtctgtttg gaaatctgat cgctctgagt ctgggcctga ctcctaactt
     3061 caaaagcaat tttgatctgg ctgaggacgc caaactgcag ctgtcaaagg acacatatga
     3121 cgatgacctg gataacctgc tggcacagat cggagatcag tacgctgacc tgttcctggc
     3181 tgccaaaaat ctgtccgacg caatcctgct gtcagatatt ctgagagtga acagcgagat
     3241 tacaaaagct cctctgagtg ccagcatgat caagagatat gacgagcacc atcaggatct
     3301 gaccctgctg aaggctctgg tcaggcagca gctgccagag aagtacaagg aaattttctt
     3361 tgatcagtcc aagaacggct acgccggtta tatcgacgga ggcgcatcac aggaggaatt
     3421 ctacaagttt atcaaaccta ttctggagaa gatggacgga actgaggaac tgctggtgaa
     3481 actgaataga gaggacctgc tgaggaagca gcgcacattt gataacggtt ccatcccaca
     3541 ccagattcat ctgggagagc tgcacgctat cctgaggcgc caggaagact tctacccctt
     3601 tctgaaagat aaccgcgaga agatcgaaaa aattctgacc ttcagaatcc cttactatgt
     3661 gggtccactg gctcgcggaa acagcagatt tgcctggatg actcgcaaat ccgaggaaac
     3721 cattactcct tggaacttcg aggaagtggt cgataagggc gcctctgcac agtccttcat
     3781 cgagagaatg actaattttg acaaaaacct gcccaatgag aaagtgctgc ctaagcactc
     3841 cctgctgtac gagtatttca ctgtctataa cgaactgaca aaggtgaaat acgtcaccga
     3901 gggcatgaga aagccagcct tcctgtcagg agagcagaag aaagcaatcg tggatctgct
     3961 gtttaaaacc aacaggaaag tgactgtcaa gcagctgaag gaggactact tcaagaaaat
     4021 tgaatgcttc gattccgtgg agatcagcgg agtcgaagac agatttaacg caagcctggg
     4081 cgcttaccac gatctgctga agatcattaa ggataaagac ttcctggaca acgaggaaaa
     4141 tgaggatatc ctggaagaca ttgtgctgac actgaccctg tttgaggaca gaggaatgat
     4201 cgaggaaaga ctgaaaacct atgctcatct gttcgatgac aaggtgatga aacagctgaa
     4261 gagaaggcgc tacactggct ggggtagact gagcaggaag ctgatcaacg gcattaggga
     4321 taaacagtca ggaaagacaa tcctggactt tctgaaaagt gatggcttcg ccaaccgcaa
     4381 ttttatgcag ctgattcacg atgacagtct gaccttcaaa gaggacatcc agaaggctca
     4441 ggtgtctgga cagggccact ccctgcatga gcagattgca aacctggctg gaagcccagc
     4501 catcaagaaa ggcattctgc agacagtgaa aatcgtcgat gagctggtga aagtcatggg
     4561 ccataagccc gaaaacatcg tgattgagat ggctcgcgaa aatcagacaa cccagaaggg
     4621 tcagaagaac agtagagaga ggatgaaaag aatcgaggaa ggcattaagg agctgggtag
     4681 ccagatcctg aaagagcacc cagtggaaaa cacacagctg cagaatgaga agctgtatct
     4741 gtactatctg cagaatggaa gagatatgta cgtggaccag gagctggata ttaacaggct
     4801 gtctgattac gacgtggatc atatcgtccc ccagagtttc atcaaagatg acagcattga
     4861 caacaaggtg ctgaccaggt ccgacaaaaa cagaggaaaa tcagataatg tccctagtga
     4921 ggaagtggtc aagaaaatga agaactactg gagacagctg ctgaatgcca aactgatcac
     4981 tcagaggaag tttgataacc tgacaaaagc agagcgcggt ggactgtcag aactggacaa
     5041 agctggattc atcaagaggc agctggtgga aacacgccag atcactaaac acgtcgcaca
     5101 gattctggat agtcgcatga acacaaagta cgatgagaat gacaaactga tcagagaagt
     5161 gaaggtcatt accctgaaga gtaaactggt cagcgacttt aggaaagatt tccagtttta
     5221 taaggtccgc gagattaaca attatcacca tgcccatgac gcatacctga acgccgtggt
     5281 cggtaccgca ctgatcaaga aatacccaaa actggagagc gaattcgtgt acggagacta
     5341 taaggtgtac gatgtcagaa aaatgatcgc caagtccgag caggaaattg gaaaagctac
     5401 tgccaagtat ttcttttact caaacatcat gaatttcttt aagacagaga tcaccctggc
     5461 caatggagaa atccgcaaaa ggcccctgat tgagacaaac ggagagacag gcgaaatcgt
     5521 gtgggacaaa ggcagagatt ttgcaaccgt gaggaaggtc ctgagcatgc ctcaagtgaa
     5581 tatcgtcaag aaaactgagg tgcagacagg cggtttctca aaagaaagta ttctgccaaa
     5641 acgcaactct gataagctga tcgctagaaa gaaagactgg gaccctaaga agtatggagg
     5701 ctttgactct cccactgtgg catactccgt cctggtggtc gctaaggtgg agaagggcaa
     5761 aagcaagaaa ctgaaatctg tcaaggagct gctgggtatc acaattatgg agagaagctc
     5821 tttcgagaag aacccaatcg attttctgga ggccaaaggt tataaggaag tgaagaaaga
     5881 cctgatcatt aaactgccca agtacagtct gtttgagctg gaaaacggca ggaaacgcat
     5941 gctggcaagc gctggagagc tgcagaaagg caatgaactg gccctgcctt ctaagtacgt
     6001 gaacttcctg tatctggcaa gccactacga gaagctgaaa ggatctccag aggataacga
     6061 acagaaacag ctgtttgtgg agcagcacaa gcattatctg gacgagatca ttgaacagat
     6121 tagcgagttc tctaaaagag tgatcctggc cgacgcaaat ctggataagg tcctgtctgc
     6181 ttacaacaaa cacagagata agcccatcag ggagcaggcc gaaaatatca ttcatctgtt
     6241 cactctgaca aacctgggcg cacctgcagc tttcaagtac ttcgacacta caatcgatag
     6301 aaagaggtac acctccacta aggaggtgct ggacgctaca ctgatccatc agagtattac
     6361 cggcctgtac gaaacaagga ttgacctgtc tcagctgggt ggcgactcac cggtgagatc
     6421 tcctaagaag aagagaaagg tgaggtccgg cggcggagag ggcagaggaa gtcttctaac
     6481 atgcggtgac gtggaggaga atcccggccc aatggccaat ttactgaccg tacaccaaaa
     6541 tttgcctgca ttaccggtcg atgcaacgag tgatgaggtt cgcaagaacc tgatggacat
     6601 gttcagggat caccaggcgt tttctgagca tacctggaaa atgcttctgt ccgtttgccg
     6661 gtcgtgggcg gcatggtgca agttgaataa ccggaaatgg tttcccgcag aacctgaaga
     6721 tgttcgcgat tatcttctat atcttcaggc gcgcggtctg gcagtaaaaa ctatccagca
     6781 acatttgggc cagctaaaca tgcttcatcg tcggtccggg ctgccacgac caagtgacag
     6841 caatgctgtt tcactggtta tgcggcggat ccgaaaagaa aacgttgatg ccggtgaacg
     6901 tgcaaaacag gctctagcgt tcgaacgcac tgatttcgac caggttcgtt cactcatgga
     6961 aaatagcgat cgctgccagg atatacgtaa tctggcattt ctggggattg cttataacac
     7021 cctgttacgt atagccgaaa ttgccaggat cagggttaaa gatatctcac gtactgacgg
     7081 tgggagaatg ttaatccata ttggcagaac gaaaacgctg gttagcaccg caggtgtaga
     7141 gaaggcactt agcctggggg taactaaact ggtcgagcga tggatttccg tgtctggtgt
     7201 agctgatgat ccgaataact acctgttttg ccgggtcaga aaaaatggtg ttgccgcgcc
     7261 atctgccacc agccagctat caactcgcgc cctggaaggg atttttgaag caactcatcg
     7321 attgatttac ggcgctaagg atgactctgg tcagagatac ctggcctggt ctggacacag
     7381 tgcccgtgtc ggagccgcgc gagatatggc ccgcgctgga gtttcaatac cggagatcat
     7441 gcaagctggt ggctggacca atgtaaatat tgtcatgaac tatatccgta acctggatag
     7501 tgaaacaggg gcaatggtgc gcctgctgga agatggcgat tagtctagaa ctatagtgag
     7561 tcgtattacg tagatccaga catgataaga tacattgatg agtttggaca aaccacaact
     7621 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
     7681 accattataa gctgcaataa accttgtaca aagtggggga tcctacgctt acagctccaa
     7741 aactctacgg taaacctaca taaactgctg gttttcaaat tttaaagaat ttaagggttt
     7801 acaggtttac tactacacag tgatttactg acacatgtaa gtgtaaatga gttgaataag
     7861 taagtaagcc atataccaca catgaaacac atacccagaa gtcactggta tatatagccg
     7921 tcctccagac tcccagctcg agacgattag atctaatgcg tctcgtttaa gagctatgct
     7981 ggaaacagca tagcaagttt aaataaggct agtccgttat caacttgaaa aagtggcacc
     8041 gagtcggtgc tttttttcaa tgatgaatta attcgagctc caccgcggaa gcttaaacaa
     8101 gaatctctag ttttctttct tgcttttact tttacttcct taatactcaa gtacaatttt
     8161 aatggagtac ttttttactt ttactcaagt aagattctag ccagatactt ttacttttaa
     8221 ttgagtaaaa ttttccctaa gtacttgtac tttcacttga gtaaaatttt tgagtacttt
     8281 ttacacctct gctcgaccat atgggagagc tcccaacgcg ttggatgcat agcttgagta
     8341 ttctatagtg tcacctaaat agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     8401 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     8461 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     8521 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     8581 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     8641 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     8701 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     8761 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     8821 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     8881 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     8941 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     9001 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     9061 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     9121 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     9181 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     9241 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     9301 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     9361 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     9421 cacgttaagg cattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     9481 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     9541 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     9601 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     9661 gtgctgcaat gataccgcga gatccacgct caccggctcc agatttatca gcaataaacc
     9721 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     9781 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     9841 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     9901 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     9961 ttagctcctt cggtcctccg atc
//
