LOCUS Exported 7757 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Clostridial vector, encoding the Cre protein, which mediates recombination between lox sites.. ACCESSION . VERSION . KEYWORDS pQcre1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7757) AUTHORS Cerisy T, Rostain W, Chhun A, Boutard M, Salanoubat M, Tolonen AC TITLE A Targetron-Recombinase System for Large-Scale Genome Engineering of Clostridia. JOURNAL mSphere. 2019 Dec 11;4(6). pii: 4/6/e00710-19. doi: 10.1128/mSphere.00710-19. PUBMED 31826971 REFERENCE 2 (bases 1 to 7757) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7757 /organism="synthetic DNA construct" /mol_type="other DNA" rep_origin 386..974 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 875..894 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 1128..1145 /label=L4440 /note="L4440 vector, forward primer" protein_bind 1262..1283 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 1298..1328 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 1336..1352 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 1341..1363 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 1360..1376 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 1360..1376 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" CDS complement(1422..2453) /codon_start=1 /product="site-specific recombinase" /label=Cre /note="Cre is a site-specific recombinase from bacteriophage P1. Recombination occurs at loxP sequences." /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE DGD" primer_bind complement(2639..2656) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(2639..2655) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2648..2670) /label=M13/pUC Forward /note="In lacZ gene" CDS complement(2981..3352) /codon_start=1 /gene="traJ" /product="oriT-recognizing protein" /label=traJ /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE KQDELGKVMMGVVRPRAEP" oriT 3385..3494 /note="incP origin of transfer" ORIGIN 1 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg 61 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta 121 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg 181 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata 241 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt 301 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc 361 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct 421 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa 481 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag 541 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc 601 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg 661 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca 721 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat 781 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg 841 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc 901 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc 961 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc 1021 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg 1081 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga 1141 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc 1201 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa 1261 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc 1321 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg 1381 attacgccaa gcttgcatgc ctgcaggtcg actctagatt actaatcgcc atcttccagc 1441 aggcgcacca ttgcccctgt ttcactatcc aggttacgga tatagttcat gacaatattt 1501 acattggtcc agccaccagc ttgcatgatc tccggtattg aaactccagc gcgggccata 1561 tctcgcgcgg ctccgacacg ggcactgtgt ccagaccagg ccaggtatct ctgaccagag 1621 tcatccttag cgccgtaaat caatcgatga gttgcttcaa aaatcccttc cagggcgcga 1681 gttgatagct ggctggtggc agatggcgcg gcaacaccat tttttctgac ccggcaaaac 1741 aggtagttat tcggatcatc agctacacca gagacggaaa tccatcgctc gaccagttta 1801 gttaccccca ggctaagtgc cttctctaca cctgcggtgc taaccagcgt tttcgttctg 1861 ccaatatgga ttaacatcct cccaccgtca gtacgtgaga tatctttaac cctgatcctg 1921 gcaatttcgg ctatacgtaa cagggtgtta taagcaatcc ccagaaatgc cagattacgt 1981 atatcctggc agcgatcgct attttccatg agtgaacgaa cctggtcgaa atcagtgcgt 2041 tcgaacgcta gagcctgttt tgcacgttca ccggcatcaa cgttttcttt tcggatccgc 2101 cgcataacca gtgaaacagc attgctgtca cttggtcgtg gcagcccgga ccgacgatga 2161 agcatgttta gctggcccaa atgttgctgg atagttttta ctgccagacc gcgcgcctga 2221 agatatagaa gataatcgcg aacatcttca ggttctgcgg gaaaccattt ccggttattc 2281 aacttgcacc atgccgccca cgaccggcaa acggacagaa gcattttcca ggtatgctca 2341 gaaaacgcct ggcgatccct gaacatgtcc atcaggttct tgcgaacctc atcactcgtt 2401 gcatcgaccg gtaatgcagg caaattttgg tgtacggtca gtaaattgga catatgcgtt 2461 ctcctttttg tataaaatta aatttatatt atattgaaat taataaactt tagtttcaat 2521 ataatataaa taaaaagaga taattattta tttttggctt tttaaacaga acttttataa 2581 atatataaca taaaaaagaa tttatatgtt taataggatg aatccgaacc tcgaattcac 2641 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc 2701 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc 2761 cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta 2821 cgcatctgtg cggtatttca caccgcatag gggatcggtc ttgccttgct cgtcggtgat 2881 gtacttcacc agctccgcga agtcgctctt cttgatggag cgcatgggga cgtgcttggc 2941 aatcacgcgc accccccggc cgttttagcg gctaaaaaag tcatggctct gccctcgggc 3001 ggaccacgcc catcatgacc ttgccaagct cgtcctgctt ctcttcgatc ttcgccagca 3061 gggcgaggat cgtggcatca ccgaaccgcg ccgtgcgcgg gtcgtcggtg agccagagtt 3121 tcagcaggcc gcccaggcgg cccaggtcgc cattgatgcg ggccagctcg cggacgtgct 3181 catagtccac gacgcccgtg attttgtagc cctggccgac ggccagcagg taggccgaca 3241 ggctcatgcc ggccgccgcc gccttttcct caatcgctct tcgttcgtct ggaaggcagt 3301 acaccttgat aggtgggctg cccttcctgg ttggcttggt ttcatcagcc atccgcttgc 3361 cctcatctgt tacgccggcg gtagccggcc agcctcgcag agcaggattc ccgttgagca 3421 ccgccaggtg cgaataaggg acagtgaaga aggaacaccc gctcgcgggt gggcctactt 3481 cacctatcct gcccggctga cgccgttgga tacaccaagg aaagtctaca cgaacccttt 3541 ggcaaaatcc tgtatatcgt gcgaaaaagg atggatatac cgaaaaaatc gctataatga 3601 ccccgaagca gggttatgca gcggaaaaga tcccccgaag caaacttaag agtgtgttga 3661 tagtgcagta tcttaaaatt ttgtgtataa taggaattga agttaaatta gatgctaaaa 3721 atttgtaatt aagaaggagg gattcgtcat gttggtattc caaatgcgta atgtagataa 3781 aacatctact gttttgaaac agactaaaaa cagtgattac gcagataaat aaatacgtta 3841 gattaattcc taccagtgac taatcttatg actttttaaa cagataacta aaattacaaa 3901 caaatcgttt aacttctgta tttatttaca gatgtaatca cttcaggagt aattacatga 3961 acaaaaatat aaaatattct caaaactttt taacgagtga aaaagtactc aaccaaataa 4021 taaaacaatt gaatttaaaa gaaaccgata ccgtttacga aattggaaca ggtaaagggc 4081 atttaacgac gaaactggct aaaataagta aacaggtaac gtctattgaa ttagacagtc 4141 atctattcaa cttatcgtca gaaaaattaa aactgaacat tcgtgtcact ttaattcacc 4201 aagatattct acagtttcaa ttccctaaca aacagaggta taaaattgtt gggagtattc 4261 cttaccattt aagcacacaa attattaaaa aagtggtttt tgaaagccat gcgtctgaca 4321 tctatctgat tgttgaagaa ggattctaca agcgtacctt ggatattcac cgaacactag 4381 ggttgctctt gcacactcaa gtctcgattc agcaattgct taagctgcca gcggaatgct 4441 ttcatcctaa accaaaagta aacagtgtct taataaaact tacccgccat accacagatg 4501 ttccagataa atattggaag ctatatacgt actttgtttc aaaatgggtc aatcgagaat 4561 atcgtcaact gtttactaaa aatcagtttc atcaagcaat gaaacacgcc aaagtaaaca 4621 atttaagtac cattacttat gagcaagtat tgtctatttt taatagttat ctattattta 4681 acgggaggaa ataattctat gagtcgcttt tttaaatttg gaaagttaca cgttactaaa 4741 gggaatggag ataaattatt agatatacta ctgacagctt ccaagaagct aaagaggtcc 4801 ctagcgccta cggggaattt gggcaaatca aaagaaaaat tctcccaaga attagaaatg 4861 agtagatcaa attattcacg aatagaatca ggaaaatcag atccaaccat aaaaacacta 4921 gaacaaattg caaagttaac taactcaacg ctagtagtgg atttaatccc aaatgagcca 4981 acagaaccag aaccagaaac agaatcagaa caagtaacat tggatttaga aatggaagaa 5041 gaaaaaagca atgacttcgt gtgaataatg cacgaaatcg ttgcttattt tttttaaaag 5101 cggtatacta gatataacga aacaacgaac tgaatagaaa cgaaaaaaga gccatgacac 5161 atttataaaa tgtttgacga cattttataa atgcatagcc cgataagatt gccaaaccaa 5221 cgcttatcag ttagtcagat gaactcttcc ctcgtaagaa gttatttaat taactttgtt 5281 tgaagacggt atataaccgt actatcatta tatagggaaa tcagagagtt ttcaagtatc 5341 taagctactg aatttaagaa ttgttaagca atcaatcgga aatcgtttga ttgctttttt 5401 tgtattcatt tatagaaggt ggagtttgta tgaatcatga tgaatgtaaa acttatataa 5461 aaaatagttt attggagata agaaaattag caaatatcta tacactagaa acgtttaaga 5521 aagagttaga aaagagaaat atctacttag aaacaaaatc agataagtat ttttcttcgg 5581 agggggaaga ttatatatat aagttaatag aaaataacaa aataatttat tcgattagtg 5641 gaaaaaaatt gacttataaa ggaaaaaaat ctttttcaaa acatgcaata ttgaaacagt 5701 tgaatgaaaa agcaaaccaa gttaattaaa caacctattt tataggattt ataggaaagg 5761 agaacagctg aatgaatatc ccttttgttg tagaaactgt gcttcatgac ggcttgttaa 5821 agtacaaatt taaaaatagt aaaattcgct caatcactac caagccaggt aaaagcaaag 5881 gggctatttt tgcgtatcgc tcaaaatcaa gcatgattgg cggtcgtggt gttgttctga 5941 cttccgagga agcgattcaa gaaaatcaag atacatttac acattggaca cccaacgttt 6001 atcgttatgg aacgtatgca gacgaaaacc gttcatacac gaaaggacat tctgaaaaca 6061 atttaagaca aatcaatacc ttctttattg attttgatat tcacacggca aaagaaacta 6121 tttcagcaag cgatatttta acaaccgcta ttgatttagg ttttatgcct actatgatta 6181 tcaaatctga taaaggttat caagcatatt ttgttttaga aacgccagtc tatgtgactt 6241 caaaatcaga atttaaatct gtcaaagcag ccaaaataat ttcgcaaaat atccgagaat 6301 attttggaaa gtctttgcca gttgatctaa cgtgtaatca ttttggtatt gctcgcatac 6361 caagaacgga caatgtagaa ttttttgatc ctaattaccg ttattctttc aaagaatggc 6421 aagattggtc tttcaaacaa acagataata agggctttac tcgttcaagt ctaacggttt 6481 taagcggtac agaaggcaaa aaacaagtag atgaaccctg gtttaatctc ttattgcacg 6541 aaacgaaatt ttcaggagaa aagggtttaa tagggcgtaa taacgtcatg tttaccctct 6601 ctttagccta ctttagttca ggctattcaa tcgaaacgtg cgaatataat atgtttgagt 6661 ttaataatcg attagatcaa cccttagaag aaaaagaagt aatcaaaatt gttagaagtg 6721 cctattcaga aaactatcaa ggggctaata gggaatacat taccattctt tgcaaagctt 6781 gggtatcaag tgatttaacc agtaaagatt tatttgtccg tcaagggtgg tttaaattca 6841 agaaaaaaag aagcgaacgt caacgtgttc atttgtcaga atggaaagaa gatttaatgg 6901 cttatattag cgaaaaaagc gatgtataca agccttattt agtgacgacc aaaaaagaga 6961 ttagagaagt gctaggcatt cctgaacgga cattagataa attgctgaag gtactgaagg 7021 cgaatcagga aattttcttt aagattaaac caggaagaaa tggtggcatt caacttgcta 7081 gtgttaaatc attgttgcta tcgatcatta aagtaaaaaa agaagaaaaa gaaagctata 7141 taaaggcgct gacaaattct tttgacttag agcatacatt cattcaagag actttaaaca 7201 agctagcaga acgccctaaa acggacacac aactcgattt gtttagctat gatacaggct 7261 gaaaataaaa cccgcactat gccattacat ttatatctat gatacgtgtt tgttttttct 7321 ttgctgttta gcgaatgatt agcagaaata tacagagtaa gattttaatt aattattagg 7381 gggagaagga gagagtagcc cgaaaacttt tagttggctt ggactgaacg aagtgaggga 7441 aaggctacta aaacgtcgag gggcagtgag agcgaagcga acacttgatt ttttaatttt 7501 ctatctttta taggtcatta gagtatactt atttgtccta taaactattt agcagcataa 7561 tagatttatt gaataggtca tttaagttga gcatattaga ggaggaaaat cttggagaaa 7621 tatttgaaga acccgattac atggattgga ttagttcttg tggttacgtg gtttttaact 7681 aaaagtagtg aatttttgat ttttggtgtg tgtgtcttgt tgttagtatt tgctagtcaa 7741 agtgattaaa tagaatt //