LOCUS       Exported                9543 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Express EBFP with an upstream T7 promoter in a lentiviral backbone.
ACCESSION   .
VERSION     .
KEYWORDS    pLX - T7pro - EBFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9543)
  AUTHORS   Chen H, Liu S, Padula S, Lesman D, Griswold K, Lin A, Zhao T, 
            Marshall JL, Chen F
  TITLE     Efficient, continuous mutagenesis in human cells using a 
            pseudo-random DNA editor.
  JOURNAL   Nat Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0331-8. doi: 
            10.1038/s41587-019-0331-8.
  PUBMED    31844291
REFERENCE   2  (bases 1 to 9543)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9543
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        96..1274
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          327..1265
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1222..1242
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        1294..1312
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1371..2090
                     /codon_start=1
                     /product="enhanced blue variant of GFP"
                     /label=EBFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTHGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1416..1437)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1677..1696)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2024..2045
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2141..2729
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2194..2214)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2612..2623)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2801..3034
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3112..3233
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3149..3168)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3203..3222
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3273..3408
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(3429..3447)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3457..3474)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3457..3473)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3466..3488)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3615..4070
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3702..3721)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3912..3933
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4096..4200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4201..5061
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4419..4438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5232..5820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5721..5740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5974..5991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6108..6129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6144..6174
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6182..6198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6187..6209
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6206..6222
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6206..6222
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6241..6261
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6243..6261
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6289..6515
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6516..6696
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6743..6868
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7361..7594
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7779..7823
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8054..8264
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8076..8272
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8123..8258
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8294..9313
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     "
     misc_feature    9384..9501
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctcgaggcct gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct
       61 aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag
      121 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg
      181 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
      241 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
      301 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
      361 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga
      421 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag
      481 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc
      541 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa
      601 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc
      661 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt
      721 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg
      781 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc
      841 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc
      901 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg
      961 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact
     1021 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt
     1081 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg
     1141 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg
     1201 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat
     1261 ttcaggtgtc gtgaggctag catcgattga tcataatacg actcactata gggagaccca
     1321 agctggctag cgtttaaact taagcttggt accgagctcg gatccactag atggtgagca
     1381 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     1441 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     1501 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     1561 ccctgaccca cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     1621 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     1681 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     1741 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     1801 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     1861 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     1921 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     1981 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     2041 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtga tagtaatgaa
     2101 ctagtaccgg ttaagtcgac aatcaacgcg ttaagtcgac aatcaacctc tggattacaa
     2161 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     2221 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     2281 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     2341 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     2401 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     2461 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     2521 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
     2581 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     2641 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     2701 tcggatctcc ctttgggccg cctccccgcg tcgactttaa gaccaatgac ttacaaggca
     2761 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     2821 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     2881 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     2941 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     3001 agaccctttt agtcagtgtg gaaaatctct agcagtacgt atagtagttc atgtcatctt
     3061 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt
     3121 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     3181 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     3241 tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     3301 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     3361 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc
     3421 caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg
     3481 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
     3541 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
     3601 gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     3661 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     3721 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     3781 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     3841 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     3901 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     3961 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     4021 ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact
     4081 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     4141 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     4201 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     4261 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     4321 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     4381 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     4441 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     4501 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     4561 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     4621 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     4681 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     4741 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     4801 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     4861 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     4921 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     4981 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     5041 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     5101 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     5161 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     5221 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     5281 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     5341 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     5401 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     5461 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     5521 ttaccggata aggcgcagcg atcgggctga acggggggtt cgtgcacaca gcccagcttg
     5581 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     5641 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     5701 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     5761 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     5821 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     5881 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     5941 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     6001 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     6061 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     6121 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     6181 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc
     6241 gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg
     6301 caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag
     6361 agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag
     6421 gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag
     6481 atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct
     6541 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     6601 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     6661 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
     6721 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
     6781 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
     6841 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     6901 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     6961 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     7021 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     7081 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     7141 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     7201 gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     7261 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     7321 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     7381 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     7441 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     7501 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     7561 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     7621 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     7681 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     7741 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     7801 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     7861 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     7921 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     7981 caaccccgag gggacccatg cattgcatct caattagtca gcaaccaggt gtggaaagtc
     8041 cccaggctcc ccagcaggca gaagtatgca aagcatgcgt ctcaattagt cagcaaccat
     8101 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     8161 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     8221 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctttc
     8281 tagaggtacc accatgaaaa agcctgaact caccgctacc tctgtcgaga agtttctgat
     8341 cgaaaagttc gacagcgtct ccgacctgat gcagctctcc gagggcgaag aatctcgggc
     8401 tttcagcttc gatgtgggag ggcgtggata tgtcctgcgg gtgaatagct gcgccgatgg
     8461 tttctacaaa gatcgctatg tttatcggca ctttgcatcc gccgctctcc ctattcccga
     8521 agtgcttgac attggggagt tcagcgagag cctgacctat tgcatctccc gccgtgcaca
     8581 gggtgtcacc ttgcaagacc tgcctgaaac cgaactgccc gctgttctcc agcccgtcgc
     8641 cgaggccatg gatgccatcg ctgccgccga tcttagccag accagcgggt tcggcccatt
     8701 cggacctcaa ggaatcggtc aatacactac atggcgcgat ttcatctgcg ctattgctga
     8761 tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgcct ccgtcgccca
     8821 ggctctcgat gagctgatgc tttgggccga ggactgcccc gaagtccggc acctcgtgca
     8881 cgccgatttc ggctccaaca atgtcctgac cgacaatggc cgcataacag ccgtcattga
     8941 ctggagcgag gccatgttcg gggattccca atacgaggtc gccaacatct tcttctggag
     9001 gccctggttg gcttgtatgg agcagcagac ccgctacttc gagcggaggc atcccgagct
     9061 tgcaggatct cctcggctcc gggcttatat gctccgcatt ggtcttgacc aactctatca
     9121 gagcttggtt gacggcaatt tcgatgatgc agcttgggct cagggtcgct gcgacgcaat
     9181 cgtccggtcc ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa gcgctgccgt
     9241 ctggaccgat ggctgtgtgg aagtgctcgc cgatagtgga aacagacgcc ccagcactcg
     9301 tcctagggca aaggatctgc agtaatgagg cgcgccgtta acgaattcta gatcttgaga
     9361 caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca
     9421 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa
     9481 attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggcgc
     9541 cgg
//
