LOCUS       Exported               10614 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Dox-inducible polycistronic reprogramming lentiviral vector 
            expressing mouse Klf4, Sox2, cMyc.
ACCESSION   .
VERSION     .
KEYWORDS    pHAGE2-TetOminiCMV-KSM
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10614)
  AUTHORS   Velychko S, Adachi K, Kim KP, Hou Y, MacCarthy CM, Wu G, Scholer HR
  TITLE     Excluding Oct4 from Yamanaka Cocktail Unleashes the Developmental 
            Potential of iPSCs.
  JOURNAL   Cell Stem Cell. 2019 Oct 30. pii: S1934-5909(19)30423-0. doi: 
            10.1016/j.stem.2019.10.002.
  PUBMED    31708402
REFERENCE   2  (bases 1 to 10614)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10614
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2251..2521
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     primer_bind     2589..2613
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2679..2688
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     misc_feature    4113..4680
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4130..4682
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4274..4291)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4501..4520
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5663..5722
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     misc_feature    7056..7644
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7109..7129)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7527..7538)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7792..7972
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8019..8037)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8105..8209
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8210..9070
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8428..8447)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9241..9829
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9730..9749
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9983..10000
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     10092..10111
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     protein_bind    10240..10261
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        10276..10306
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10314..10330
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10319..10341
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10338..10354
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10338..10354
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10404..10423
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcca
     2221 caccctaact gacacactcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
     2281 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
     2341 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt
     2401 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac
     2461 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
     2521 aaaagtgaaa gtcgagctcg gtacccgggt cgaggtaggc atgtacggtg ggaggcctat
     2581 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
     2641 gacctccata gaagacaccg ggaccgatcc agcctgcggc cgccatggct gtcagcgacg
     2701 ctctgctccc gtccttctcc acgttcgcgt ccggcccggc gggaagggag aagacactgc
     2761 gtccagcagg tgccccgact aaccgttggc gtgaggaact ctctcacatg aagcgacttc
     2821 ccccacttcc cggccgcccc tacgacctgg cggcgacggt ggccacagac ctggagagtg
     2881 gcggagctgg tgcagcttgc agcagtaaca acccggccct cctagcccgg agggagaccg
     2941 aggagttcaa cgacctcctg gacctagact ttatcctttc caactcgcta acccaccagg
     3001 aatcggtggc cgccaccgtg accacctcgg cgtcagcttc atcctcgtct tccccagcga
     3061 gcagcggccc tgccagcgcg ccctccacct gcagcttcag ctatccgatc cgggccgggg
     3121 gtgacccggg cgtggctgcc agcaacacag gtggagggct cctctacagc cgagaatctg
     3181 cgccacctcc cacggccccc ttcaacctgg cggacatcaa tgacgtgagc ccctcgggcg
     3241 gcttcgtggc tgagctcctg cggccggagt tggacccagt atacattccg ccacagcagc
     3301 ctcagccgcc aggtggcggg ctgatgggca agtttgtgct gaaggcgtct ctgaccaccc
     3361 ctggcagcga gtacagcagc ccttcggtca tcagtgttag caaaggaagc ccagacggca
     3421 gccaccccgt ggtagtggcg ccctacagcg gtggcccgcc gcgcatgtgc cccaagatta
     3481 agcaagaggc ggtcccgtcc tgcacggtca gccggtccct agaggcccat ttgagcgctg
     3541 gaccccagct cagcaacggc caccggccca acacacacga cttccccctg gggcggcagc
     3601 tccccaccag gactacccct acactgagtc ccgaggaact gctgaacagc agggactgtc
     3661 accctggcct gcctcttccc ccaggattcc atccccatcc ggggcccaac taccctcctt
     3721 tcctgccaga ccagatgcag tcacaagtcc cctctctcca ttatcaagag ctcatgccac
     3781 cgggttcctg cctgccagag gagcccaagc caaagagggg aagaaggtcg tggccccgga
     3841 aaagaacagc cacccacact tgtgactatg caggctgtgg caaaacctat accaagagtt
     3901 ctcatctcaa ggcacacctg cgaactcaca caggcgagaa accttaccac tgtgactggg
     3961 acggctgtgg gtggaaattc gcccgctcgg atgaactgac caggcactac cgcaaacaca
     4021 cagggcaccg gccctttcag tgccagaagt gcgacagggc cttttccagg tcggaccacc
     4081 ttgccttaca catgaagagg cacttttaaa gatccctccc ccccccctaa cgttactggc
     4141 cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg
     4201 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct
     4261 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca
     4321 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg
     4381 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct
     4441 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa
     4501 tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt
     4561 atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa
     4621 aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa
     4681 tatggccaca catatgatgt ataacatgat ggagacggag ctgaagccgc cgggcccgca
     4741 gcaagcttcg gggggcggcg gcggaggagg caacgccacg gcggcggcaa ccggcggcaa
     4801 ccagaagaac agcccggacc gcgtcaagag gcccatgaac gccttcatgg tatggtcccg
     4861 ggggcagcgg cgtaagatgg cccaggagaa ccccaagatg cacaactcgg agatcagcaa
     4921 gcgcctgggc gcggagtgga aacttttgtc cgagaccgag aagcggccgt tcatcgacga
     4981 ggccaagcgg ctgcgcgctc tgcacatgaa ggagcacccg gattataaat accggccgcg
     5041 gcggaaaacc aagacgctca tgaagaagga taagtacacg cttcccggag gcttgctggc
     5101 ccccggcggg aacagcatgg cgagcggggt tggggtgggc gccggcctgg gtgcgggcgt
     5161 gaaccagcgc atggacagct acgcgcacat gaacggctgg agcaacggca gctacagcat
     5221 gatgcaggag cagctgggct acccgcagca cccgggcctc aacgctcacg gcgcggcaca
     5281 gatgcaaccg atgcaccgct acgacgtcag cgccctgcag tacaactcca tgaccagctc
     5341 gcagacctac atgaacggct cgcccaccta cagcatgtcc tactcgcagc agggcacccc
     5401 cggtatggcg ctgggctcca tgggctctgt ggtcaagtcc gaggccagct ccagcccccc
     5461 cgtggttacc tcttcctccc actccagggc gccctgccag gccggggacc tccgggacat
     5521 gatcagcatg tacctccccg gcgccgaggt gccggagccc gctgcgccca gtagactgca
     5581 catggcccag cactaccaga gcggcccggt gcccggcacg gccattaacg gcacactgcc
     5641 cctgtcgcac atgggtagtg ggcaatgtac taactacgct ttgttgaaac tcgctggcga
     5701 tgttgaaagt aaccccggtc ctatgcccct caacgtgaac ttcaccaaca ggaactatga
     5761 cctcgactac gactccgtac agccctattt catctgcgac gaggaagaga atttctatca
     5821 ccagcaacag cagagcgagc tgcagccgcc cgcgcccagt gaggatatct ggaagaaatt
     5881 cgagctgctt cccaccccgc ccctgtcccc gagccgccgc tccgggctct gctctccatc
     5941 ctatgttgcg gtcgctacgt ccttctcccc aagggaagac gatgacggcg gcggtggcaa
     6001 cttctccacc gccgatcagc tggagatgat gaccgagtta cttggaggag acatggtgaa
     6061 ccagagcttc atctgcgatc ctgacgacga gaccttcatc aagaacatca tcatccagga
     6121 ctgtatgtgg agcggtttct cagccgctgc caagctggtc tcggagaagc tggcctccta
     6181 ccaggctgcg cgcaaagaca gcaccagcct gagccccgcc cgcgggcaca gcgtctgctc
     6241 cacctccagc ctgtacctgc aggacctcac cgccgccgcg tccgagtgca ttgacccctc
     6301 agtggtcttt ccctacccgc tcaacgacag cagctcgccc aaatcctgta cctcgtccga
     6361 ttccacggcc ttctctcctt cctcggactc gctgctgtcc tccgagtcct ccccacgggc
     6421 cagccctgag cccctagtgc tgcatgagga gacaccgccc accaccagca gcgactctga
     6481 agaagagcaa gaagatgagg aagaaattga tgtggtgtct gtggagaaga ggcaaacccc
     6541 tgccaagagg tcggagtcgg gctcatctcc atcccgaggc cacagcaaac ctccgcacag
     6601 cccactggtc ctcaagaggt gccacgtctc cactcaccag cacaactacg ccgcaccccc
     6661 ctccacaagg aaggactatc cagctgccaa gagggccaag ttggacagtg gcagggtcct
     6721 gaagcagatc agcaacaacc gcaagtgctc cagccccagg tcctcagaca cggaggaaaa
     6781 cgacaagagg cggacacaca acgtcttgga acgtcagagg aggaacgagc tgaagcgcag
     6841 cttttttgcc ctgcgtgacc agatccctga attggaaaac aacgaaaagg cccccaaggt
     6901 agtgatcctc aaaaaagcca ccgcctacat cctgtccatt caagcagacg agcacaagct
     6961 cacctctgaa aaggacttat tgaggaaacg acgagaacag ttgaaacaca aactcgaaca
     7021 gcttcgaaac tctggtgcat aaatcgatag atcctaatca acctctggat tacaaaattt
     7081 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     7141 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     7201 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     7261 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     7321 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     7381 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     7441 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
     7501 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     7561 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     7621 tctccctttg ggccgcctcc ccgcctggta cctttaagac caatgactta caaggcagct
     7681 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     7741 cgaagacaag atcacctgca ggacaggcgc gccctgcttt ttgcttgtac tgggtctctc
     7801 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     7861 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     7921 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cacccgggcg
     7981 attaaggaaa gggctagatc attcttgaag acgaaagggc ctcgtgatac gcctattttt
     8041 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     8101 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     8161 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     8221 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     8281 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     8341 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     8401 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc
     8461 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     8521 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     8581 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     8641 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     8701 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     8761 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     8821 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     8881 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     8941 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     9001 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     9061 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     9121 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     9181 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     9241 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     9301 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     9361 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     9421 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     9481 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     9541 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     9601 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     9661 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     9721 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     9781 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     9841 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     9901 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     9961 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
    10021 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagcaagc tcatggctga
    10081 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
    10141 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctccccgt ggcacgacag
    10201 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
    10261 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
    10321 cggataacaa tttcacacag gaaacagcta tgacatgatt acgaatttca caaataaagc
    10381 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
    10441 ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc
    10501 tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt
    10561 attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag tagt
//
