LOCUS Exported 6907 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Retroviral expression vector driving CH3 domain fragment of human p300 (EP300), with N-terminal FLAG tag.. ACCESSION . VERSION . KEYWORDS pLB(N)CX-FLAG-CH3 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6907) AUTHORS Borger DR, DeCaprio JA TITLE Targeting of p300/CREB binding protein coactivators by simian virus 40 is mediated through p53. JOURNAL J Virol. 2006 May . 80(9):4292-303. PUBMED 16611888 REFERENCE 2 (bases 1 to 6907) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6907 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 1..380 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 381..584 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 535..555 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 581..605 /label=LNCX /note="Human CMV promoter, forward primer" CDS 699..722 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" LTR 1419..2012 /note="long terminal repeat from Moloney murine leukemia virus" primer_bind complement(2115..2137) /label=pGEX 3' /note="pGEX vectors, reverse primer" misc_feature 2223..2363 /label=bom /note="basis of mobility region from pBR322" primer_bind 2278..2297 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind complement(2378..2395) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(2549..3137) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(2629..2648) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(3308..4168) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 3931..3950 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(4169..4273) /gene="bla" /label=AmpR promoter primer_bind 4341..4359 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" LTR 4931..5106 /label=5' LTR (truncated) /note="truncated long terminal repeat from Moloney murine sarcoma virus" misc_feature 5169..5368 /label=MMLV Psi /note="packaging signal of Moloney murine leukemia virus (MMLV)" CDS 5569..5985 /label=gag (truncated) /note="truncated Moloney murine leukemia virus (MMLV) gag gene lacking the start codon" primer_bind 5915..5937 /label=pLXSN 5' /note="Murine stem cell virus, forward primer. Also called MSCV" primer_bind 5953..5969 /label=pBABE 5' /note="Psi packaging signal, forward primer for pBABE vectors" CDS 6028..6426 /codon_start=1 /gene="Aspergillus terreus BSD" /product="blasticidin S deaminase" /label=BSD /note="confers resistance to blasticidin" /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI KAIVKDSDGQPTAVGIRELLPSGYVWEG" ORIGIN 1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc 61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca 121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga 181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc 241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct 301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat 361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc 421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt 481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa 541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc 601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat 661 ccagcctccg cggccccaag cttggatcct gaaccatgga ctacaaggac gacgatgaca 721 agtcggccgc tggaggaggc cttggcttag atgatgagag caacaaccag caggctgcag 781 ccacccagag cccaggcgat tctcgccgcc tgagtatcca gcgctgcatc cagtctctgg 841 tccatgcttg ccagtgtcgg aatgccaatt gctcactgcc atcctgccag aagatgaagc 901 gggttgtgca gcataccaag ggttgcaaac ggaaaaccaa tggcgggtgc cccatctgca 961 agcagctcat tgccctctgc tgctaccatg ccaagcactg ccaggagaac aaatgcccgg 1021 tgccgttctg cctaaacatc aagcagaagc tccggcagca acagctgcag caccgactac 1081 agcaggccca aatgcttcgc aggaggatgg ccagcatgca gcggactggt gtggttgggc 1141 agcaacaggg cctcccttcc cccactcctg ccactccaac gacaccaact ggccaacagc 1201 caaccacccc gcagacgccc cagcccactt ctcagcctca gcctacccct cccaatagca 1261 tgccacccta cttgcccagg actcaagctg ctggccctgt gtcccagggt aaggcagcag 1321 gccaggtgac ccctccaacc cctcctcaga cttaggaatt caagcttgtt aacatcgata 1381 aaataaaaga ttttatttag tctccagaaa aaggggggaa tgaaagaccc cacctgtagg 1441 tttggcaagc tagcttaagt aacgccattt tgcaaggcat ggaaaaatac ataactgaga 1501 atagagaagt tcagatcaag gtcaggaaca gatggaacag ctgaatatgg gccaaacagg 1561 atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg aacagctgaa 1621 tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg gccaagaaca 1681 gatggtcccc agatgcggtc cagccctcag cagtttctag agaaccatca gatgtttcca 1741 gggtgcccca aggacctgaa atgaccctgt gccttatttg aactaaccaa tcagttcgct 1801 tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat aaaagagccc acaacccctc 1861 actcggggcg ccagtcctcc gattgactga gtcgcccggg tacccgtgta tccaataaac 1921 cctcttgcag ttgcatccga cttgtggtct cgctgttcct tgggagggtc tcctctgagt 1981 gattgactac ccgtcagcgg gggtctttca tttgggggct cgtccgggat cgggagaccc 2041 ctgcccaggg accaccgacc caccaccggg aggtaagctg gctgcctcgc gcgtttcggt 2101 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa 2161 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg 2221 ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg 2281 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg 2341 taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct 2401 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca 2461 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga 2521 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc 2581 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 2641 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 2701 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt 2761 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 2821 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 2881 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 2941 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg 3001 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 3061 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca 3121 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 3181 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga 3241 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt 3301 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt 3361 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat 3421 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag 3481 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct 3541 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt 3601 tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg 3661 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca 3721 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt 3781 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat 3841 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac 3901 cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa 3961 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt 4021 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt 4081 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa 4141 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt 4201 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa 4261 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta 4321 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt caagaattca 4381 taccagatca ccgaaaactg tcctccaaat gtgtccccct cacactccca aattcgcggg 4441 cttctgcctc ttagaccact ctaccctatt ccccacactc accggagcca aagccgcggc 4501 ccttccgttt ctttgctttt gaaagacccc acccgtaggt ggcaagctag cttaagtaac 4561 gccactttgc aaggcatgga aaaatacata actgagaata gaaaagttca gatcaaggtc 4621 aggaacaaag aaacagctga ataccaaaca ggatatctgt ggtaagcggt tcctgccccg 4681 gctcagggcc aagaacagat gagacagctg agtgatgggc caaacaggat atctgtggta 4741 agcagttcct gccccggctc ggggccaaga acagatggtc cccagatgcg gtccagccct 4801 cagcagtttc tagtgaatca tcagatgttt ccagggtgcc ccaaggacct gaaaatgacc 4861 ctgtacctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc gcgcttccgc 4921 tctccgagct caataaaaga gcccacaacc cctcactcgg cgcgccagtc ttccgataga 4981 ctgcgtcgcc cgggtacccg tattcccaat aaagcctctt gctgtttgca tccgaatcgt 5041 ggtctcgctg ttccttggga gggtctcctc tgagtgattg actacccacg acgggggtct 5101 ttcatttggg ggctcgtccg ggatttggag acccctgccc agggaccacc gacccaccac 5161 cgggaggtaa gctggccagc aacttatctg tgtctgtccg attgtctagt gtctatgttt 5221 gatgttatgc gcctgcgtct gtactagtta gctaactagc tctgtatctg gcggacccgt 5281 ggtggaactg acgagttctg aacacccggc cgcaaccctg ggagacgtcc cagggacttt 5341 gggggccgtt tttgtggccc gacctgagga agggagtcga tgtggaatcc gaccccgtca 5401 ggatatgtgg ttctggtagg agacgagaac ctaaaacagt tcccgcctcc gtctgaattt 5461 ttgctttcgg tttggaaccg aagccgcgcg tcttgtctgc tgcagcgctg cagcatcgtt 5521 ctgtgttgtc tctgtctgac tgtgtttctg tatttgtctg aaaattaggg ccagactgtt 5581 accactccct taagtttgac cttaggtcac tggaaagatg tcgagcggat cgctcacaac 5641 cagtcggtag atgtcaagaa gagacgttgg gttaccttct gctctgcaga atggccaacc 5701 tttaacgtcg gatggccgcg agacggcacc tttaaccgag acctcatcac ccaggttaag 5761 atcaaggtct tttcacctgg cccgcatgga cacccagacc aggtccccta catcgtgacc 5821 tgggaagcct tggcttttga cccccctccc tgggtcaagc cctttgtaca ccctaagcct 5881 ccgcctcctc ttcctccatc cgccccgtct ctcccccttg aacctcctcg ttcgaccccg 5941 cctcgatcct ccctttatcc agccctcact ccttctctag gcgccggaat tccgatctga 6001 tcaagagaca ggatggggtc gtttcgcatg gccaagcctt tgtctcaaga agaatccacc 6061 ctcattgaaa gagcaacggc tacaatcaac agcatcccca tctctgaaga ctacagcgtc 6121 gccagcgcag ctctctctag cgacggccgc atcttcactg gtgtcaatgt atatcatttt 6181 actgggggac cttgtgcaga actcgtggtg ctgggcactg ctgctgctgc ggcagctggc 6241 aacctgactt gtatcgtcgc gatcggaaat gagaacaggg gcatcttgag cccctgcgga 6301 cggtgccgac aggtgcttct cgatctgcat cctgggatca aagccatagt gaaggacagt 6361 gatggacagc cgacggcagt tgggattcgt gaattgctgc cctctggtta tgtgtgggag 6421 ggctaagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac 6481 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg 6541 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccaccccg 6601 ggctcgatcc cctcgcgagt tggttcagct gctgcctgag gctggacgac ctcgcggagt 6661 tctaccggca gtgcaaatcc gtcggcatcc aggaaaccag cagcggctat ccgcgcatcc 6721 atgcccccga actgcaggag tggggaggca cgatggccgc tttggtcgag gcggatccgg 6781 ccattagcca tattattcat tggttatata gcataaatca atattggcta ttggccattg 6841 catacgttgt atccatatca taatatgtac atttatattg gctcatgtcc aacattaccg 6901 ccatgtt //