LOCUS       Exported                4876 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  LbCpf1 and gRNA expression plasmid for P. falciparum; no selection 
            in parasites..
ACCESSION   .
VERSION     .
KEYWORDS    pAIO-LbCpf1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4876)
  AUTHORS   Nessel T, Beck JM, Rayatpisheh S, Jami-Alahmadi Y, Wohlschlegel JA, 
            Goldberg DE, Beck JR
  TITLE     EXP1 is required for organisation of EXP2 in the intraerythrocytic 
            malaria parasite vacuole.
  JOURNAL   Cell Microbiol. 2020 Jan 28:e13168. doi: 10.1111/cmi.13168.
  PUBMED    31990132
REFERENCE   2  (bases 1 to 4876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4876
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(952..975)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(982..1029)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             complement(1030..4713)
                     /codon_start=1
                     /product="CRISPR-associated protein from Lachnospiraceae 
                     bacterium ND2006 (Zetsche et al., 2015)"
                     /label=LbCpf1
                     /translation="MSKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAE
                     DYKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKE
                     IAKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENMFS
                     EEAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEG
                     EFFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVL
                     SDRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKN
                     GPAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQ
                     LQEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKD
                     LLDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYS
                     KDKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNG
                     NYEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKL
                     IDFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLV
                     EEGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKK
                     EELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKI
                     NTEVRVLLKHDDNPYVIGIDRGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTD
                     YHSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSG
                     FKNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQ
                     NGFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALD
                     YKNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQ
                     QGDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRN
                     YEAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTS
                     VKH"
ORIGIN
        1 atatatatat atatatatat attttatatc atttaatata ttatgtgtga tataataata
       61 aatccattgt ccataaatag atgaaatagc ggccgcctac cctgaagaag aaaagtccga
      121 tgatgttgtg gacataaaga attgagctgt ctcaaaaaat gagaaaacaa acaaacaaac
      181 aaaataccat tatgcgcata tcctctgctc aatatcaatt tttctttatg ccagttttgt
      241 ttcgacacta tttgagcaac accttccctt tactatctac tactgttaca atgttatttt
      301 attcaccttt ttacaatttt atcaaaattt ttttgtatgc atataaatta tacattgtaa
      361 agccaaagtg tagataaaga acaccttgtt tatttttccc caattttttt ttttttacca
      421 tttttttatt aaccttgctc atttacctgc taatacgatt gctatatgat atcgaaattg
      481 aaggaaaaaa catcatttgt gtttatatgt ttttttttaa tttttcaact ttttttattt
      541 aactatgcta atcatattat aaacatttta aaaatcaagt ccaactattt atgaatcatt
      601 gaagagacaa cataattata acagtaaaaa taataataat aataataatt ttgtcttatt
      661 gtttcaaaca tattatgttc cataaatatc taaattttta atatggtcat aaattcatag
      721 gatttaattt atataaaata tggctattca tactagccat tttatgtgtg taaagttttt
      781 ttttctgaac aaaaaataaa atgttcataa ttttagctat ttacatgcat gtgcatgcac
      841 aaaaaaaaat atgcacacaa catacacatt tttacagtta taaatacaat caattggtat
      901 aaatatataa ataagaaaaa cgaacattaa gctgccatat ccctcgagtt acttgtcgtc
      961 gtcgtccttg tagtcggatc cctttttctt ttttgcctgg ccggcctttt tcgtggccgc
     1021 cggccttttg tgcttcacgc tggtctgggc gtactccagc cactccttgt tagagatggc
     1081 gatcttcacc ttatccagct tctcgtcctc ggccttcttg aactggccga tggcccacag
     1141 cacctttctg gcgatgttat aggcgccatt ggcgtcggcg ttctttggca ggatggcatt
     1201 ctcctgggcc tcatagttcc ggctatcgta gaagatgccg tcggagttct tcacagggct
     1261 gatcagaaaa tccacgtcgg tgcggcctgt gatgctgttc cgcatctgca gcatcaggct
     1321 catcagggcc ataaagctag agtagaaggc cttgtcggac tgctcgcaca gcagggctct
     1381 gatatcgccc tgctgataat tgatgccgta cttgttgaac agctccttat aggcgctggt
     1441 caggcacacc tcctcccagt cgaacacgtt gttcttctta ggattccgga agattctgat
     1501 ccggttgccg taggagtaca gcttccactt cttgatgtaa tcggcgtctg tgcgagagaa
     1561 gttcttatag tccagggcaa actcgaacag atcctcctcg ggcacgtaca tgatcctgtc
     1621 aaaggagctg atgaacttct tggaatcggc gatgctggta tacttggttt tcagcaggtt
     1681 cacaaagccg gtagatggat cgatcttgga tgtcagccag gcagggatgt aaaagatgaa
     1741 gccgttctgg gtagacatgg acttaaagct ctcgaactta ttggtgatct gatagccctt
     1801 cagggcgccg cctgttgcac aaggattaga cttcttgtcc accatgtagt tcagcttatc
     1861 gatcagcatc ttctcgaact tctgatacac ctgcttctcc accttcacgc ggctattctt
     1921 aaagccagag ttcaggtcct ccagggcgat cacggcatcg tacttctcca ccagctcgca
     1981 gatcttgtgc accacctgag agatatagcc ggccttcagc tccttgatat tctcgatgga
     2041 ggtccagttc tggcgggcct cgaacctctc cttctccttc ttgtccagca gagagtggta
     2101 atctgtcttg atcctgatgc cgttgaagtt gttgatgatc tcgttcaggg aatactgctc
     2161 cacgatgttg cccttgccgt ccaccaccac gatatacagc agattgcgct cgcccctatc
     2221 gatgccgatc acataggggt tatcgtcgtg cttcagcagc acgcgcacct ctgtattgat
     2281 cttgaagatg ttcttggggc acttattgat ggcgattggg atgtgcagct cgtactggtc
     2341 ctcagaaaac ctcttatcct tatacacgtc gtaggacagg gttgtggttt tcttgggatt
     2401 atctggattc ttgttggcga taggggagtt ggctgggtgc accaccagct cctccttctt
     2461 cagggaggcg cgcctcatga acagctctgc tcctccgctc agcctgatct gtccgtgatt
     2521 gttctcgtca aacagcagct tgaagtacat ggtgtgcaga ttgggtgtgc cgtgagactt
     2581 atcggaaaag tccttgttat agatctggaa catatacagc ttgccctcct ccaccagctt
     2641 atccacctcc ttcttgctgg cagactcgaa gctcacctta tagccctgct cctccacctc
     2701 tctgtaaaag ccggcgatgt ccttatactt ctctgtctca gaaaagttga aatcgtaggc
     2761 attggaccac tttggatacc gggagatgct atccttaaag aagtcgatca gcttgtgaca
     2821 gtcattcagg ttaaacatat cgcccttctt gaatgtgcca ttcttgtaga tcttctggat
     2881 gtcctcgctg gggttatagt aggccatcca cttcttagaa aagaacacct ttggcagcat
     2941 cttattaggg ccgggcagca gcttatagtt gatcttctcg taattgccgt tcacatcgtc
     3001 cttgtcgatc ttctgcaggc acttggcgta cttcttatcc atgatggcca gatagtactt
     3061 ggagccgtat ctcaggatgg tggcccgata gtctgtctcc ttatccttgt cccagccgcc
     3121 catgaactga gggttctgaa aatacagctt gaacttatcc ttagagtagg gcttctgggt
     3181 cacataattg cggatggcat cgtagatgtg gtccaccttc agcaggatgt cgtaggccag
     3241 cacaaaatcg ccatagaagg actcgtccct gtttgtctcc ttgccctcgc caaagaaggc
     3301 cttgatgtaa ttctcgaagc tcttcacaga atccagcagg tccttcatga tggccaccac
     3361 ggcgtcgttc ttcttcaggc tcttctccag cacaaaatcg gcgtcgaaca gcttctcaga
     3421 ggagccatac accttgtaga tctcatccac cttctggatg atgatctcct tcagcttctc
     3481 caccacagac agatcggcgt cggcgtactc ctgcagctgc tccagagaaa aggagccgat
     3541 cttcttgaag gactttctcc gatcgtcctc gtacttctcg gtcaccacgg ccttcttctt
     3601 caggtggata tcgtcatact cggcattcca cttgtcccgg atcacgttcc actcgccgaa
     3661 gatatccttg gagattgtgc tgatggcggg gccgttcttc acaaagatgc cggcgctaga
     3721 gtactcgtca aaattcttga acagcttctc cagcttcttg atggagctga agatctcgct
     3781 gttcttgttc agggtgtttc taaacacctc cagcacctcc tcatcggatg tatagccctc
     3841 gccgtagaag ctcagagact cccgatcgct cagcacctgc ttatacagtg gcttaaactt
     3901 aggcagcttc tgcttggttt tctgattata caggttgatg tactcgttca ggcccttgat
     3961 cttctcgccg ctctcggtca cgaagccgcc gatgatggcg ttatacacgt cgatgccctc
     4021 ctgtgtcagc acaaagttaa agaactcgcc ctcaaagaaa tcctccacat catagtcgct
     4081 gttcaggatc ttctccttga tctcctgcac ctcgtgctta tcaaagatgg cgtccacctt
     4141 ctcgaagatg tccatattag agatgtagcg ggtcagattc tcgttgatac acctgaaggc
     4201 gatggatgtg ctcttggcct cctcggaaaa catattctct ctgttatcaa agaagccggt
     4261 gaaggctgtg gtaaagccat tgaagctgtt caccagggcg atctcgtcct tatcgtccag
     4321 gaactctggc aggattgtct cgatgatatc cttcttaaac agggacttgt agccctcgtt
     4381 gcccttgaag gccttggcga tctccttccg cagattgatc tccaggttct ccagctcctt
     4441 attctccttc tcggttctgg ttttcttccg gaacaggctg atgtaattgt tcagattctt
     4501 cagcttgatg ctgtgcagca cgtcgttgat aaaagacaga tagtagcgat ccagcagctt
     4561 cttcacgccc ttataatcct cggctctctt ctcgtcctcc accagcagcc gcttattgtc
     4621 gatgttctcc tgggtcttgc ccacagggat ggccttgaac ctcagggtct tagacaggga
     4681 gtagcagttt gtaaacttct ccagcttgct catggatcct gatatatttc tattaggtat
     4741 ttattattat aaaatataaa tcttgaatga taataaataa aatattagtt attccttttc
     4801 tagtttaaaa tatacatatt ataaatatat atatatatat atatttttat tgtgacaaga
     4861 atatataatt ataaat
//
