LOCUS Exported 10416 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus. ACCESSION . VERSION . KEYWORDS pLVX-EF1alpha-SARS-CoV-2-nsp4-2xStrep-IRES-Puro SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10416) AUTHORS Gordon DE, et al. TITLE A SARS-CoV-2-Human Protein-Protein Interaction Map Reveals Drug Targets and Potential Drug Repurposing JOURNAL BioRxiv March 2020 REFERENCE 2 (bases 1 to 10416) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10416 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..634 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 681..806 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1303..1536 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1721..1765 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2028..2143 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" promoter 2338..3519 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 2568..3510 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 3467..3487 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" CDS 5071..5094 /codon_start=1 /product="peptide that binds Strep-Tactin(R), an engineered form of streptavidin" /label=Strep-Tag II /translation="WSHPQFEK" CDS 5131..5154 /codon_start=1 /product="peptide that binds Strep-Tactin(R), an engineered form of streptavidin" /label=Strep-Tag II /translation="WSHPQFEK" misc_feature 5166..5739 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(5333..5350) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 5560..5579 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 5772..6371 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(5772..5791) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 6268..6288 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 6385..6973 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(6438..6458) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(6856..6867) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 7180..7813 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" primer_bind complement(7941..7957) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(7941..7957) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(7954..7976) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 7965..7981 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(7989..8019) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 8034..8055 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(8172..8189) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(8343..8931) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(8423..8442) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(9102..9962) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 9725..9744 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(9963..10067) /gene="bla" /label=AmpR promoter polyA_signal 10115..10249 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(10152..10171) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 10206..10225 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg 2221 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc 2281 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct 2341 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag 2401 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg 2461 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag 2521 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt 2581 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt 2641 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg 2701 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg 2761 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct 2821 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg 2881 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc 2941 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga 3001 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct 3061 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca 3121 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg 3181 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg 3241 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat 3301 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg 3361 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa 3421 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca 3481 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gatctatttc cggtgaattc 3541 gccgccacca tgaagattgt aaacaattgg cttaaacaac ttattaaagt aaccctggta 3601 ttcttgtttg tcgccgcaat cttttacttg ataacaccgg tccacgtgat gtctaaacac 3661 acagacttta gtagtgagat aatcggatat aaagctatcg atggcggcgt tacccgcgac 3721 atcgcttcaa ccgacacatg tttcgctaat aagcacgccg attttgacac ttggtttagt 3781 caaagaggag gaagctacac aaacgacaag gcttgtccgc ttatcgcggc tgtaataaca 3841 agggaagttg ggtttgtcgt gcctggtttg ccaggtacta tactcagaac cacaaacggt 3901 gactttctgc acttcttgcc aagggttttt agcgcggtag gcaatatctg ctatacgcca 3961 agcaagctga ttgaatacac cgactttgcg acgagcgcat gtgtcctcgc tgcagagtgt 4021 acgatattta aggatgcgag cggaaagccc gtaccttact gctatgatac taatgtcctt 4081 gagggatcag ttgcgtatga gagtcttagg ccggatacac gatatgtgct catggatggt 4141 tccataatcc agttcccaaa tacctacctc gaagggtccg ttcgggtcgt caccacattt 4201 gatagcgaat attgcagaca cggaacatgc gaacggagtg aagcaggggt ctgcgtctcc 4261 acaagtgggc ggtgggtcct caataacgat tattaccgga gcctgcccgg cgtgttttgc 4321 ggggtcgatg cggttaactt gttgacgaat atgtttacgc ctctcataca acccattggc 4381 gcactggata tatctgcaag tatagtcgct ggaggcattg tggcaatcgt ggttacgtgt 4441 ttggcgtatt attttatgag gttccgccgg gctttcgggg aatattccca cgtagtcgcc 4501 ttcaacacac tcttgtttct catgtctttc actgtactgt gtttgactcc tgtctactct 4561 ttcttgccgg gtgtatactc agttatttac ttgtacctca cgttttattt gacaaacgat 4621 gtttcatttc tcgcgcatat ccaatggatg gtgatgttta cgccattggt gcctttctgg 4681 atcacgatcg cttatatcat ttgcatcagc accaaacact tctattggtt ctttagtaac 4741 tacttgaagc gcagggttgt ttttaacggt gtcagcttct caacgtttga agaggcagca 4801 ctgtgtactt tccttctgaa taaggaaatg tatctcaagc tgcggtccga tgtcctgctt 4861 ccgcttaccc agtataatcg ctatctcgcc ctgtataata agtacaagta cttctcaggc 4921 gcgatggata caaccagtta tagagaggct gcctgctgcc atcttgccaa agctttgaat 4981 gacttcagca atagtggctc agacgttctc taccaaccac cgcaaacttc cataacctca 5041 gccgtactcc agctcgaagg cggcggggga tggagccatc cacaattcga gaaaggcggt 5101 ggttcaggag gaggtagcgg gggtggatca tggtcacatc cgcagtttga aaagtaagga 5161 tcccgcccct ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc 5221 cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg 5281 gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc 5341 aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga 5401 agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg 5461 tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag 5521 tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc 5581 aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct 5641 cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac 5701 cacggggacg tggttttcct ttgaaaaaca cgatgataag cttgccacaa cccacaagga 5761 gacgaccttc catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc 5821 cccgggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg 5881 tcgacccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg 5941 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga 6001 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg 6061 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc 6121 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca 6181 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc 6241 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca 6301 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc 6361 ccggtgccta gacgcgtctg gaacaatcaa cctctggatt acaaaatttg tgaaagattg 6421 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct 6481 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg 6541 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact 6601 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc 6661 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc 6721 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag 6781 ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc 6841 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg 6901 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg 6961 gccgcctccc cgcctggaat taattctgca gtcgagacct agaaaaacat ggagcaatca 7021 caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg 7081 aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag 7141 ctgtagatct tagccacttt ttaaaagaaa agaggggact ggaagggcta attcactccc 7201 aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt 7261 agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca 7321 agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct 7381 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga 7441 ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca 7501 agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc 7561 gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt 7621 tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa 7681 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt 7741 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg 7801 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag 7861 aaatgaatat cagagagtga gaggccttga cattgctagc gtttaccgtc gacctctagc 7921 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca 7981 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg 8041 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg 8101 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc 8161 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta 8221 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag 8281 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg 8341 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg 8401 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg 8461 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga 8521 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc 8581 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt 8641 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact 8701 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg 8761 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt 8821 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt 8881 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct 8941 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg 9001 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt 9061 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt 9121 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc 9181 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg 9241 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc 9301 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg 9361 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca 9421 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga 9481 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct 9541 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg 9601 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca 9661 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata 9721 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct 9781 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact 9841 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa 9901 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc 9961 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga 10021 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga 10081 aaagtgccac ctgacgtcga cggatcggga gatcaacttg tttattgcag cttataatgg 10141 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc 10201 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac 10261 tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta 10321 cctgtggttt catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa 10381 ttgtatttgt taaatatgta ctacaaactt agtagt //