LOCUS Exported 6610 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Retroviral vector with CMV promoter driving mutant form of CH3 domain fragment of human p300 (EP300), with N-terminal FLAG tag.. ACCESSION . VERSION . KEYWORDS pLB(N)CX-FLAG-CH3 Del33 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6610) AUTHORS Borger DR, DeCaprio JA TITLE Targeting of p300/CREB binding protein coactivators by simian virus 40 is mediated through p53. JOURNAL J Virol. 2006 May . 80(9):4292-303. PUBMED 16611888 REFERENCE 2 (bases 1 to 6610) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6610 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 414..589 /label=5' LTR (truncated) /note="truncated long terminal repeat from Moloney murine sarcoma virus" misc_feature 652..851 /label=MMLV Psi /note="packaging signal of Moloney murine leukemia virus (MMLV)" CDS 1052..1468 /label=gag (truncated) /note="truncated Moloney murine leukemia virus (MMLV) gag gene lacking the start codon" primer_bind 1398..1420 /label=pLXSN 5' /note="Murine stem cell virus, forward primer. Also called MSCV" primer_bind 1436..1452 /label=pBABE 5' /note="Psi packaging signal, forward primer for pBABE vectors" CDS 1511..1909 /codon_start=1 /gene="Aspergillus terreus BSD" /product="blasticidin S deaminase" /label=BSD /note="confers resistance to blasticidin" /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI KAIVKDSDGQPTAVGIRELLPSGYVWEG" enhancer 2391..2770 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 2771..2974 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 2925..2945 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 2971..2995 /label=LNCX /note="Human CMV promoter, forward primer" CDS 3089..3112 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" LTR 3512..4105 /note="long terminal repeat from Moloney murine leukemia virus" primer_bind complement(4208..4230) /label=pGEX 3' /note="pGEX vectors, reverse primer" misc_feature 4316..4456 /label=bom /note="basis of mobility region from pBR322" primer_bind 4371..4390 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind complement(4471..4488) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(4642..5230) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(4722..4741) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(5401..6261) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 6024..6043 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(6262..6366) /gene="bla" /label=AmpR promoter primer_bind 6434..6452 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" ORIGIN 1 tttgaaagac cccacccgta ggtggcaagc tagcttaagt aacgccactt tgcaaggcat 61 ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca aagaaacagc 121 tgaataccaa acaggatatc tgtggtaagc ggttcctgcc ccggctcagg gccaagaaca 181 gatgagacag ctgagtgatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg 241 ctcggggcca agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagtgaa 301 tcatcagatg tttccagggt gccccaagga cctgaaaatg accctgtacc ttatttgaac 361 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc cgctctccga gctcaataaa 421 agagcccaca acccctcact cggcgcgcca gtcttccgat agactgcgtc gcccgggtac 481 ccgtattccc aataaagcct cttgctgttt gcatccgaat cgtggtctcg ctgttccttg 541 ggagggtctc ctctgagtga ttgactaccc acgacggggg tctttcattt gggggctcgt 601 ccgggatttg gagacccctg cccagggacc accgacccac caccgggagg taagctggcc 661 agcaacttat ctgtgtctgt ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg 721 tctgtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt 781 ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc gtttttgtgg 841 cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg tggttctggt 901 aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggaa 961 ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct 1021 gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc ccttaagttt 1081 gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa 1141 gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg tcggatggcc 1201 gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg tcttttcacc 1261 tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag ccttggcttt 1321 tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc ctcttcctcc 1381 atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta 1441 tccagccctc actccttctc taggcgccgg aattccgatc tgatcaagag acaggatggg 1501 gtcgtttcgc atggccaagc ctttgtctca agaagaatcc accctcattg aaagagcaac 1561 ggctacaatc aacagcatcc ccatctctga agactacagc gtcgccagcg cagctctctc 1621 tagcgacggc cgcatcttca ctggtgtcaa tgtatatcat tttactgggg gaccttgtgc 1681 agaactcgtg gtgctgggca ctgctgctgc tgcggcagct ggcaacctga cttgtatcgt 1741 cgcgatcgga aatgagaaca ggggcatctt gagcccctgc ggacggtgcc gacaggtgct 1801 tctcgatctg catcctggga tcaaagccat agtgaaggac agtgatggac agccgacggc 1861 agttgggatt cgtgaattgc tgccctctgg ttatgtgtgg gagggctaag cgggactctg 1921 gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc 1981 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc 2041 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccgggctcga tcccctcgcg 2101 agttggttca gctgctgcct gaggctggac gacctcgcgg agttctaccg gcagtgcaaa 2161 tccgtcggca tccaggaaac cagcagcggc tatccgcgca tccatgcccc cgaactgcag 2221 gagtggggag gcacgatggc cgctttggtc gaggcggatc cggccattag ccatattatt 2281 cattggttat atagcataaa tcaatattgg ctattggcca ttgcatacgt tgtatccata 2341 tcataatatg tacatttata ttggctcatg tccaacatta ccgccatgtt gacattgatt 2401 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga 2461 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg 2521 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg 2581 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca 2641 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc 2701 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc 2761 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc 2821 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa 2881 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag 2941 gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg 3001 gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccg 3061 cggccccaag cttggatcct gaaccatgga ctacaaggac gacgatgaca agtcggccgc 3121 tggaggaggc cttggcttag atgatgagag caacaaccag caggctgcag ccacccagag 3181 cccaggcgat tctcgccgcc tgagtatcca gcagcggact ggtgtggttg ggcagcaaca 3241 gggcctccct tcccccactc ctgccactcc aacgacacca actggccaac agccaaccac 3301 cccgcagacg ccccagccca cttctcagcc tcagcctacc cctcccaata gcatgccacc 3361 ctacttgccc aggactcaag ctgctggccc tgtgtcccag ggtaaggcag caggccaggt 3421 gacccctcca acccctcctc agacttagga attcaagctt gttaacatcg ataaaataaa 3481 agattttatt tagtctccag aaaaaggggg gaatgaaaga ccccacctgt aggtttggca 3541 agctagctta agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaga 3601 agttcagatc aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg 3661 tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc 3721 caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc 3781 cccagatgcg gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc 3841 ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct 3901 tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggg 3961 gcgccagtcc tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg 4021 cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac 4081 tacccgtcag cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca 4141 gggaccaccg acccaccacc gggaggtaag ctggctgcct cgcgcgtttc ggtgatgacg 4201 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg 4261 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag 4321 ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga 4381 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag 4441 aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt 4501 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 4561 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 4621 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 4681 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 4741 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 4801 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 4861 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 4921 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 4981 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 5041 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg 5101 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 5161 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 5221 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 5281 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt 5341 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag 5401 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat 5461 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc 5521 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa 5581 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca 5641 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa 5701 cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt 5761 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc 5821 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact 5881 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc 5941 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg 6001 ctcttgcccg gcgtcaacac gggataatac cgcgccacat agcagaactt taaaagtgct 6061 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc 6121 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag 6181 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac 6241 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg 6301 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt 6361 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac 6421 attaacctat aaaaataggc gtatcacgag gccctttcgt cttcaagaat tcataccaga 6481 tcaccgaaaa ctgtcctcca aatgtgtccc cctcacactc ccaaattcgc gggcttctgc 6541 ctcttagacc actctaccct attccccaca ctcaccggag ccaaagccgc ggcccttccg 6601 tttctttgct //