LOCUS Exported 13395 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Mammalian expression of the centrosomal protein SAS6 N-terminally fused to CLIP-tag. ACCESSION . VERSION . KEYWORDS pEBTetDBl-CLIP-HsSAS6 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 13395) AUTHORS Lukinavicius G, Lavogina D, Orpinell M, Umezawa K, Reymond L, Garin N, Gonczy P, Johnsson K TITLE Selective chemical crosslinking reveals a Cep57-Cep63-Cep152 centrosomal complex. JOURNAL Curr Biol. 2013 Feb 4;23(3):265-70. doi: 10.1016/j.cub.2012.12.030. Epub 2013 Jan 17. PUBMED 23333316 REFERENCE 2 (bases 1 to 13395) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..13395 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(2404..4329) /codon_start=1 /product="Epstein-Barr nuclear antigen 1, also known as EBNA-1" /label=EBNA1 /note="crucial for latent viral infection, and for episomal amplification of vectors containing the oriP origin (Okita et al., 2013)" /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG AGGGAGAGGGAGGAGGAGGAGAGGGAG?GGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA GGAGGAGAGGGAGAGGG?GG?G?GGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG G?GGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE GEEGQE" primer_bind complement(4764..4782) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 4850..4954 /gene="bla" /label=AmpR promoter CDS 4955..5815 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(5173..5192) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 5986..6574 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6475..6494 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind complement(6763..6783) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 6781..7078 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind 7010..7029 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 7100..7498 /codon_start=1 /gene="Aspergillus terreus BSD" /product="blasticidin S deaminase" /label=BSD /note="confers resistance to blasticidin" /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI KAIVKDSDGQPTAVGIRELLPSGYVWEG" polyA_signal 7542..7676 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(7814..7833) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" promoter 8110..8371 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" intron 8488..9060 /label=-beta-globin intron /note="intron from rabbit beta-globin gene" primer_bind 9068..9087 /label=pCAG-F /note="Rabbit beta-globin intron, for pCAG plasmids, forward primer" primer_bind 9115..9134 /label=T7 /note="T7 promoter, forward primer" promoter 9115..9133 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 9144..9761 /codon_start=1 /gene="tetR from transposon Tn10" /product="tetracycline repressor TetR" /label=TetR /note="TetR binds to the tetracycline operator tetO to inhibit transcription. This inhibition can be relieved by adding tetracycline or doxycycline." /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESG" primer_bind complement(9217..9236) /label=Tet-R /note="Tetracycline resistance gene, reverse primer" polyA_signal 9806..9927 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" enhancer 9982..10361 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 10362..10565 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 10516..10536 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" protein_bind 10567..10585 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 10588..10606 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" primer_bind 10616..10640 /label=LNCX /note="Human CMV promoter, forward primer" CDS 10740..10763 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" CDS 10764..10781 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" CDS 11331..11354 /codon_start=1 /product="recognition and cleavage site for human rhinovirus 3C and PreScission proteases" /label=HRV 3C site /translation="LEVLFQGP" protein_bind 11367..11391 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(13385..14) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" ORIGIN 1 ttgtacaaag tggtgatggc agctagcggc gcgcccggcc gcggggcagt gcatgtaatc 61 ccttcagttg gttggtacaa cttgccaact gggccctgtt ccacatgtga cacggggggg 121 gaccaaacac aaaggggttc tctgactgta gttgacatcc ttataaatgg atgtgcacat 181 ttgccaacac tgagtggctt tcatcctgga gcagactttg cagtctgtgg actgcaacac 241 aacattgcct ttatgtgtaa ctcttggctg aagctcttac accaatgctg ggggacatgt 301 acctcccagg ggcccaggaa gactacggga ggctacacca acgtcaatca gaggggcctg 361 tgtagctacc gataagcgga ccctcaagag ggcattagca atagtgttta taaggccccc 421 ttgttaaccc taaacgggta gcatatgctt cccgggtagt agtatatact atccagacta 481 accctaattc aatagcatat gttacccaac gggaagcata tgctatcgaa ttagggttag 541 taaaagggtc ctaaggaaca gcgatatctc ccaccccatg agctgtcacg gttttattta 601 catggggtca ggattccacg agggtagtga accattttag tcacaagggc agtggctgaa 661 gatcaaggag cgggcagtga actctcctga atcttcgcct gcttcttcat tctccttcgt 721 ttagctaata gaataactgc tgagttgtga acagtaaggt gtatgtgagg tgctcgaaaa 781 caaggtttca ggtgacgccc ccagaataaa atttggacgg ggggttcagt ggtggcattg 841 tgctatgaca ccaatataac cctcacaaac cccttgggca ataaatacta gtgtaggaat 901 gaaacattct gaatatcttt aacaatagaa atccatgggg tggggacaag ccgtaaagac 961 tggatgtcca tctcacacga atttatggct atgggcaaca cataatccta gtgcaatatg 1021 atactggggt tattaagatg tgtcccaggc agggaccaag acaggtgaac catgttgtta 1081 cactctattt gtaacaaggg gaaagagagt ggacgccgac agcagcggac tccactggtt 1141 gtctctaaca cccccgaaaa ttaaacgggg ctccacgcca atggggccca taaacaaaga 1201 caagtggcca ctcttttttt tgaaattgtg gagtgggggc acgcgtcagc ccccacacgc 1261 cgccctgcgg ttttggactg taaaataagg gtgtaataac ttggctgatt gtaaccccgc 1321 taaccactgc ggtcaaacca cttgcccaca aaaccactaa tggcaccccg gggaatacct 1381 gcataagtag gtgggcgggc caagataggg gcgcgattgc tgcgatctgg aggacaaatt 1441 acacacactt gcgcctgagc gccaagcaca gggttgttgg tcctcatatt cacgaggtcg 1501 ctgagagcac ggtgggctaa tgttgccatg ggtagcatat actacccaaa tatctggata 1561 gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta tatctgggta 1621 gtatatgcta tcctaatctg tatccgggta gcatatgcta tcctaataga gattagggta 1681 gtatatgcta tcctaattta tatctgggta gcatatacta cccaaatatc tggatagcat 1741 atgctatcct aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagcat 1801 aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat 1861 atgctatcct aatttatatc tgggtagcat aggctatcct aatctatatc tgggtagcat 1921 atgctatcct aatctatatc tgggtagtat atgctatcct aatctgtatc cgggtagcat 1981 atgctatcct catgcatata cagtcagcat atgataccca gtagtagagt gggagtgcta 2041 tcctttgcat atgccgccac ctcccaaggg ggcgtgaatt ttcgctgctt gtccttttcc 2101 tgctggttgc tcccattctt aggtgaattt aaggaggcca ggctaaagcc gtcgcatgtc 2161 tgattgctca ccaggtaaat gtcgctaatg ttttccaacg cgagaaggtg ttgagcgcgg 2221 agctgagtga cgtgacaaca tgggtatgcc caattgcccc atgttgggag gacgaaaatg 2281 gtgacaagac agatggccag aaatacacca acagcacgca tgatgtctac tggggattta 2341 ttctttagtg cgggggaata cacggctttt aatacgattg agggcgtctc ctaacaagtt 2401 acatcactcc tgcccttcct caccctcatc tccatcacct ccttcatctc cgtcatctcc 2461 gtcatcaccc tccgcggcag ccccttccac cataggtgga aaccagggag gcaaatctac 2521 tccatcgtca aagctgcaca cagtcaccct gatattgcag gtaggagcgg gctttgtcat 2581 aacaaggtcc ttaatcgcat ccttcaaaac ctcagcaaat atatgagttt gtaaaaagac 2641 catgaaataa cagacaatgg actcccttag cgggccaggt tgtgggccgg gtccaggggc 2701 cattccaaag gggagacgac tcaatggtgt aagacgacat tgtggaatag caagggcagt 2761 tcctcgcctt aggttgtaaa gggaggtctt actacctcca tatacgaaca caccggcgac 2821 ccaagttcct tcgtcggtag tcctttctac gtgactccta gccaggagag ctcttaaacc 2881 ttctgcaatg ttctcaaatt tcgggttgga acctccttga ccacgatgct ttccaaacca 2941 ccctcctttt ttgcgcctgc ctccatcacc ctgaccccgg ggtccagtgc ttgggccttc 3001 tcctgggtca tctgcggggc cctgctctat cgctcccggg ggcacgtcag gctcaccatc 3061 tgggccacct tcttggtggt attcaaaata atcggcttcc cctacagggt ggaaaaatgg 3121 ccttctacct ggagggggcc tgcgcggtgg agacccggat gatgatgact gactactggg 3181 actcctgggc ctcttttctc cacgtccacg acctctcccc ctggctcttt cacgacttcc 3241 ccccctggct ctttcacgtc ctctaccccg gcggcctcca ctacctcctc gaccccggcc 3301 tccactacct cctcgacccc ggcctccact gcctcctcga ccccggcctc cacctcctgc 3361 tcctgcccct cctgctcctg cccctcctcc tgctcctgcc cctcctgccc ctcctgctcc 3421 tgcccctcct gcccctcctg ctcctgcccc tcctgcccct cctgctcctg cccctcctgc 3481 ccctcctcct gctcctgccc ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc 3541 tgctcctgcc cctcctgccc ctcctgctcc tgcccctcct gcccctcctg ctcctgcccc 3601 tcctgctcct gcccctcctg ctcctgcccc tcctgctcct gcccctcctg cccctcctsc 3661 ccctcctcct gctcctgccc ctcctgctcc tgcccctcct gcccctcctg cccctcctgc 3721 tcctgcccct cctcctgctc ctgcccctcc tgcccctcct gcycctcctc ctsctcctsc 3781 ccctcctscc cctcctcctg ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc 3841 tgcccctcct cctgctcctg cccctcctgc ccctcctcct gctcctgccc ctcctcctgc 3901 tcctgcccct cctgcccctc ctgcccctcc tcctsctcct gcccctcctc ctgctcctgc 3961 ccctcctgcc cctcctgccc ctcctgcccc tcctcctgct cctgcccctc ctcctgctcc 4021 tgcccctcct gctcctgccc ctcccgctcc tgctcctgct cctgttccac cgtgggtccc 4081 tttgcagcca atgcaacttg gacgtttttg gggtctccgg acaccatctc tatgtcttgg 4141 ccctgatcct gagccgcccg gggctcctgg tcttccgcct cctcgtcctc gtcctcttcc 4201 ccgtcctcgt ccatggttat caccccctct tctttgaggt ccactgccgc cggagccttc 4261 tggtccagat gtgtctccct tctctcctag gccatttcca ggtcctgtac ctggcccctc 4321 gtcagacatg attcacacta aaagagatca atagacatct ttattagacg acgctcagtg 4381 aatacaggga gtgcagactc ctgccccctc caacagcccc cccaccctca tccccttcat 4441 ggtcgctgtc agacagatcc aggtctgaaa attccccatc ctccgaacca tcctcgtcct 4501 catcaccaat tactcgcagc ccggaaaact cccgctgaac atcctcaaga tttgcgtcct 4561 gagcctcaag ccaggcctca aattcctcgt cccccttttt gctggacggt agggatgggg 4621 attctcggga cccctcctct tcctcttcaa ggtcaccaga cagagatgct actggggcaa 4681 cggaagaaaa gctgggtgcg gcctgtgagg atcagcttat cgatgataag ctgtcaaaca 4741 tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat 4801 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc 4861 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg 4921 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc 4981 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt 5041 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct 5101 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac 5161 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact 5221 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa 5281 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga 5341 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt 5401 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga 5461 agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg 5521 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat 5581 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat 5641 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc 5701 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga 5761 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc 5821 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag 5881 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc 5941 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt 6001 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt 6061 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat 6121 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc 6181 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa 6241 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg 6301 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag 6361 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag 6421 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa 6481 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt 6541 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg 6601 gttcctggcc ttttgctggc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga 6661 cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg 6721 gaaggccatc cagcctcgcg tcgaactaga tgatccggct gtggaatgtg tgtcagttag 6781 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt 6841 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca 6901 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa 6961 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag 7021 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag 7081 ggtgaccgcc acgaccggta tggccaagcc tttgtctcaa gaagaatcca ccctcattga 7141 aagagcaacg gctacaatca acagcatccc catctctgaa gactacagcg tcgccagcgc 7201 agctctctct agcgacggcc gcatcttcac tggtgtcaat gtatatcatt ttactggggg 7261 accttgtgca gaactcgtgg tgctgggcac tgctgctgct gcggcagctg gcaacctgac 7321 ttgtatcgtc gcgatcggaa atgagaacag gggcatcttg agcccctgcg gacggtgccg 7381 acaggtgctt ctcgatctgc atcctgggat caaagccata gtgaaggaca gtgatggaca 7441 gccgacggca gttgggattc gtgaattgct gccctctggt tatgtgtggg agggctaacg 7501 gtccgacgcg gcccgacggg tccgaggggg gtcgacctcg aaacttgttt attgcagctt 7561 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac 7621 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatctgat 7681 ccgaacccct tcctcgacca attctcatgt ttgacagctt atcatcgcag atccgggcaa 7741 cgttgttgcc attgctcgac caattctcat gtttgacagc ttatcatcgc agatccgtat 7801 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg 7861 cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag 7921 gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg 7981 atgtacgggc cagatatacg cgtatctgag gggactaggg tgtgtttagg cgaaaagcgg 8041 ggcttcggtt gtacgcggtt aggagtcccc tcaggatata gtagtttcgc ttttgcatag 8101 ggagggggaa atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag 8161 ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag 8221 gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac 8281 cactgaattc cgcattgcag agatattgta tttaagtgcc tagctcgata caataaacgc 8341 catttgacca ttcaccacat tggtgtgcac ctccaagctg gccggggatc ctctagggcc 8401 tctgagctat tccagaagta gtgaagaggc ttttttggag gcctaggctt ttgcaaaaag 8461 ctccggatcg atcctgagaa cttcagggtg agtttgggga cccttgattg ttctttcttt 8521 ttcgctattg taaaattcat gttatatgga gggggcaaag ttttcagggt gttgtttaga 8581 atgggaagat gtcccttgta tcaccatgga ccctcatgat aattttgttt ctttcacttt 8641 ctactctgtt gacaaccatt gtctcctctt attttctttt cattttctgt aactttttcg 8701 ttaaacttta gcttgcattt gtaacgaatt tttaaattca cttttgttta tttgtcagat 8761 tgtaagtact ttctctaatc actttttttt caaggcaatc agggtatatt atattgtact 8821 tcagcacagt tttagagaac aattgttata attaaatgat aaggtagaat atttctgcat 8881 ataaattctg gctggcgtgg aaatattctt attggtagaa acaactacat cctggtcatc 8941 atcctgcctt tctctttatg gttacaatga tatacactgt ttgagatgag gataaaatac 9001 tctgagtcca aaccgggccc ctctgctaac catgttcatg ccttcttctt tttcctacag 9061 ctcctgggca acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attgtaatac 9121 gactcactat agggcgaatt gatatgtcta gattagataa aagtaaagtg attaacagcg 9181 cattagagct gcttaatgag gtcggaatcg aaggtttaac aacccgtaaa ctcgcccaga 9241 agctaggtgt agagcagcct acattgtatt ggcatgtaaa aaataagcgg gctttgctcg 9301 acgccttagc cattgagatg ttagataggc accatactca cttttgccct ttagaagggg 9361 aaagctggca agatttttta cgtaataacg ctaaaagttt tagatgtgct ttactaagtc 9421 atcgcgatgg agcaaaagta catttaggta cacggcctac agaaaaacag tatgaaactc 9481 tcgaaaatca attagccttt ttatgccaac aaggtttttc actagagaat gcattatatg 9541 cactcagcgc tgtggggcat tttactttag gttgcgtatt ggaagatcaa gagcatcaag 9601 tcgctaaaga agaaagggaa acacctacta ctgatagtat gccgccatta ttacgacaag 9661 ctatcgaatt atttgatcac caaggtgcag agccagcctt cttattcggc cttgaattga 9721 tcatatgcgg attagaaaaa caacttaaat gtgaaagtgg gtccgcgtac agcggatccc 9781 gggaattcag atcttattaa agcagaactt gtttattgca gcttataatg gttacaaata 9841 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg 9901 tttgtccaaa ctcatcaatg tatcttatca tgtctggtcg aatccatggc gcagaactgg 9961 taggtatgga agatccgcgt tgacattgat tattgactag ttattaatag taatcaatta 10021 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg 10081 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc 10141 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa 10201 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca 10261 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta 10321 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 10381 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 10441 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 10501 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 10561 gagctctccc tatcagtgat agagatctcc ctatcagtga tagagatcgt cgacgagctc 10621 gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa 10681 gacaccggga ccgatccagc ctccggactc tagcgtttaa acttaagctt accaccatgg 10741 attataaaga cgatgatgac aagcaccacc accaccacca cgacaaggat tgtgaaatga 10801 aacgcaccac actggacagc cctttgggga agctggagct gtctggttgt gagcagggtc 10861 tgcacgaaat aatttttctg ggcaagggga cgtctgcagc tgatgccgtg gaggtcccag 10921 cccccgctgc ggttctcgga ggtccggagc ccctgataca ggctacagcc tggctgaatg 10981 cctatttcca ccagcccgag gctatcgaag agttccccgt gccggctctt caccatcccg 11041 ttttccagca agagtcgttc accagacagg tgttatggaa gctgctgaag gttgtgaaat 11101 tcggagaagt gatttctgag tcacacttag cagccctggt aggcaacccc gcagccacgg 11161 cagcagtgaa tacggcactg gatggcaatc ctgtccctat cctgatcccg tgccacagag 11221 tggtccaggg ggattcggat gtggggccgt accttggtgg actggccgtg aaggaatggc 11281 ttctggccca tgaaggccac cggttgggga agccaggctt gggattcgaa ctggaggtgc 11341 tgttccaggg ccccggtacc gccatcacaa gtttgtacaa aaaagcaggc tttatcaccg 11401 gtaccatgag ccaagtgctg ttccaccaac tagtcccgtt gcaggtgaaa tgcaaagact 11461 gtgaggagag gagagtaagt ataagaatga gcattgaact acaatcagtt tctaatccag 11521 ttcacagaaa ggacttagtt attcgtctga ctgatgacac ggatccattt tttttatata 11581 accttgttat atctgaggaa gattttcaaa gtttaaaatt ccagcaaggt cttctggtag 11641 acttcttagc tttcccacaa aaatttatag atctccttca gcaatgtact caagaacatg 11701 ccaaagaaat tccaaggttt ttgctacagt tagtttctcc agcagctatt ttggataact 11761 cacctgcatt tttaaatgtg gtagagacaa atccttttaa gcatcttaca cacctctcac 11821 taaaactttt acctggaaat gatgtggaga taaagaaatt tctcgcaggc tgtttgaaat 11881 gtagcaagga agaaaaatta tcattgatgc aatcactaga tgatgctact aagcaactgg 11941 actttacacg aaagacatta gcagaaaaaa aacaagagct cgataagtta cggaatgaat 12001 gggcgtcaca tacagcagcc ttgacaaaca agcattctca ggaactgaca aatgaaaagg 12061 aaaaagcctt gcaggcacag gttcaatatc aacagcagca tgaacaacag aaaaaagatt 12121 tagaaatcct ccatcaacaa aacatccacc agctacaaaa cagactgtct gagttagaag 12181 cggctaataa agacttaacc gaaagaaaat ataaaggaga ctccactatt agagaactta 12241 aagcaaaact ttctggtgtt gaagaggagc tacagcggac taagcaagaa gtcctctctt 12301 tgcgaagaga gaattctaca ctagatgttg aatgccacga gaaagaaaag cacgttaatc 12361 agctacaaac aaaagtggca gttttagaac aggaaatcaa ggataaggac cagcttgttt 12421 taagaacaaa agaggcattt gatacaatcc aggaacaaaa ggtggtttta gaagaaaatg 12481 gtgagaaaaa tcaagtacaa ctaggaaagc ttgaagctac aataaaatca ttatctgcag 12541 aacttctgaa ggcaaatgaa attatcaaga agttacaagg ggatctgaaa actttaatgg 12601 gtaagttgaa attgaagaat acagttacta ttcagcaaga aaaactcttg gctgagaagg 12661 aggaaaaatt acaaaaggaa caaaaggaat tacaagatgt tggacagtct cttcgaatta 12721 aagagcaaga ggtatgcaaa ttacaagaac aattagaagc tacagttaaa aaacttgaag 12781 aaagcaaaca acttctaaaa aataatgaaa agttaatcac gtggttaaat aaagaactaa 12841 atgaaaatca gctagtgaga aagcaagatg tattgggacc ttctactact ccgcctgcac 12901 attccagcag caacacaatc agaagtggaa tttctcctaa cctgaatgtg gttgatggta 12961 gactgactta cccaacctgt gggattggtt atcctgtctc ctctgcattt gcattccaga 13021 ataccttccc tcattcgata tctgccaaaa ataccagcca ccctggttca ggaacaaagg 13081 ttcagtttaa tttgcagttt acaaaaccaa atgcatcact aggagatgtt cagtcaggag 13141 caactattag tatgccttgc tcaactgata aggaaaatgg tgaaaatgta gggttggaat 13201 ccaaatacct gaagaaaagg gaagatagca ttcctttacg cggactcagc cagaacctat 13261 ttagtaattc agaccatcag agagatggca ctttaggagc attacataca tcttccaaac 13321 ccacagcgct cccctctgcg tcttcagcct atttccctgg gcagttacca aacagttaat 13381 ctagacccag ctttc //