LOCUS       Exported                 970 bp ds-DNA     linear   SYN 22-MAY-2021
DEFINITION  Gateway-compatible Entry vector, with insert of NSP14 gene's CDS 
            from SARS-CoV-2 isolate Wuhan-Hu-1.
ACCESSION   .
VERSION     .
KEYWORDS    pDONR223 SARS-CoV-2 NSP14
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 22, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..970
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    68..92
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gattttattt tgactgatag tgacctgttc gttgcaacaa attgatgagc aatgcttttt
       61 tataatgcca actttgtaca aaaaagttgg catggctgag aacgtgaccg gtctgttcaa
      121 ggactgctct aaggtcatca ctggtctgca cccaactcag gctccaaccc acctgtctgt
      181 ggacaccaag ttcaagaccg aaggactgtg cgtggacatc cccggtatcc ctaaggacat
      241 gacctaccgc aggctgatct ccatgatggg cttcaagatg aactaccagg tgaacggata
      301 ccctaacatg ttcatcacca gagaggaagc tatccgccac gtgagggcct ggatcggttt
      361 cgacgtggaa ggttgccacg ctaccagaga agctgtgggt accaacctgc cactgcagct
      421 gggcttctct accggagtga acctggtggc tgtgcctacc ggctacgtgg acaccccaaa
      481 caacaccgac ttctctagag tgagcgccaa gccccctcca ggagaccagt tcaagcacct
      541 gatccccctg atgtacaagg gtctgccttg gaacgtggtg cgcatcaaga tcgtgcagat
      601 gctgtccgac accctgaaga acctgtctga cagggtggtg ttcgtgctgt gggctcacgg
      661 cttcgagctg accagcatga agtacttcgt gaagatcgga cccgaacgta cctgctgcct
      721 gtgcgacaga cgtgccacct gcttctccac cgcctctgac acctacgctt gctggcacca
      781 ctccatcgga ttcgactacg tgtacaaccc tttcatgatc gacgtgcagc agtggggatt
      841 caccggtaac ctgcagtcca accacgacct gtactgccag gtgcacggta acgcccacgt
      901 ggcttcttgc gacgctatca tgaccagatg cctggccgtg cacgagtgct tcgtgaagcg
      961 tgtggactgg
//
