LOCUS       Exported                 899 bp ds-DNA     linear   SYN 17-MAY-2021
DEFINITION  Gateway-compatible Entry vector, with insert of NSP5 gene's CDS from
            SARS-CoV-2 isolate Wuhan-Hu-1.
ACCESSION   .
VERSION     .
KEYWORDS    pDONR223 SARS-CoV-2 NSP5
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 17, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..899
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    67..91
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgatgagca atgctttttt
       61 ataatgccaa ctttgtacaa aaaagttggc atgtccggat tccgcaagat ggccttccct
      121 tctggcaagg tggagggctg catggtgcag gtgacctgcg gaaccaccac cctgaacggt
      181 ctgtggctgg acgacgtggt gtactgcccc aggcacgtga tctgcacctc tgaggacatg
      241 ctgaacccta actacgaaga cctgctgatc agaaagagca accacaactt cctggtgcag
      301 gccggtaacg tgcagctgcg tgtgatcggc cactctatgc agaactgcgt gctgaagctg
      361 aaggtggaca ccgctaaccc caagacccct aagtacaagt tcgtgagaat ccagcctggt
      421 cagaccttct ccgtgctggc ctgctacaac ggaagcccat ccggcgtgta ccagtgcgct
      481 atgcgtccca acttcaccat caagggcagc ttcctgaacg gctcttgcgg aagcgtgggt
      541 ttcaacatcg actacgactg cgtgtccttc tgctacatgc accacatgga gctgccaact
      601 ggagtgcacg ctggaactga cctggagggt aacttctacg gccccttcgt ggacagacag
      661 actgctcagg ctgctggcac cgacaccacc atcaccgtga acgtgctggc ttggctgtac
      721 gccgctgtga tcaacggaga ccgctggttc ctgaacaggt tcaccaccac cctgaacgac
      781 ttcaacctgg tggctatgaa gtacaactac gagcctctga cccaggacca cgtggatatc
      841 ctgggtccac tgagcgccca gactggaatc gctgtgctgg acatgtgcgc ctccctgaa
//
