LOCUS       Exported                 639 bp ds-DNA     linear   SYN 12-JUN-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    TrHKII-pGFPN3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 639)
  AUTHORS   Sun L, Shukair S, Naik TJ, Moazed F, Ardehali H
  TITLE     Glucose phosphorylation and mitochondrial binding are required for 
            the protective effects of hexokinases I and II.
  JOURNAL   Mol Cell Biol. 2008 Feb . 28(3):1007-17.
  PUBMED    18039843
REFERENCE   2  (bases 1 to 639)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..639
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      17..26
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     RBS             322..330
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 tcctcgccct tgctcaccat ggtggcgatg gatcctcnct gtccagcctc acggatgcgg
       61 caggccacag cagtgatgag cgccgccccc ttcccgctgc cntcctctga ctgcaggaaa
      121 gacacatcac atttcggagc caggtccttc actgtctcat gcatgacttt ggnaaagtga
      181 ggatgtanct tgtagagggt cccatccacn cccactgtca ctttgagagc gtccagccca
      241 cggttttctc gtatcctgtc caccacagcg gccatgcctg cgccacagag ctgggctgcc
      301 cgccgggcca ccacagtgca cacctcctta acaatgatgc tgtcnncaca ngtgctctca
      361 anccctaagt gttgcaggat ggctcggact tgcancaggg ccaggcagtc actctcaatc
      421 tgagacaaga acttggtttc aaagatgccc cttgncttga gccgctctga gatgcggcct
      481 cggaagagca gtccacgctt ggtgaaatcg atgagaatgt tacngacaat ctcacccagg
      541 tacattccac tgatcatttt ctcgaacctc tgcttgcccg ggttgagtga aanntcatcc
      601 acagccacnt nnnattctgt gcggaagtca tctangcat
//
