LOCUS Exported 428 bp ds-DNA linear SYN 31-MAY-2021 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS pTriEx-mVenus-PA-Rac1-C450A SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 428) AUTHORS Wu YI, Frey D, Lungu OI, Jaehrig A, Schlichting I, Kuhlman B, Hahn KM TITLE A genetically encoded photoactivatable Rac controls the motility of living cells. JOURNAL Nature. 2009 Sep 3. 461(7260):104-8. PUBMED 19693014 REFERENCE 2 (bases 1 to 428) AUTHORS . TITLE Direct Submission JOURNAL Exported May 31, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..428 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(26..43) /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" promoter complement(64..173) /label=p10 promoter /note="baculovirus promoter for expression in insect cells" protein_bind 189..213 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind complement(213..232) /label=T7 /note="T7 promoter, forward primer" promoter complement(214..232) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(274..293) /label=pCAG-F /note="Rabbit beta-globin intron, for pCAG plasmids, forward primer" primer_bind complement(355..377) /label=pCAGGS-5 /note="Chimeric intron in CAG promoter, forward primer" ORIGIN 1 gctcctcgcc cttgctcaca gaaccgtgat ggtggtgatg gtgtgccatg gtatatctcc 61 tttgattgta aataaaatgt aatttacagt atagtatttt aattaatata caaatgattt 121 gataataatt cttatttaac tataatatat tgtgttgggt tgaattaaag gtcccggatt 181 aactccgggg aattgttatc cgctcacaat tcccctatag tgagtcgtat taatttcggt 241 ccgatccaat tctttgccaa aatgatgaga cagcacaata accagcacgt tgcccaggag 301 ctgtaggaaa aagaagaagg catgaacatg gttagcagag gctctagagc cgccggtcac 361 acgccagaag ccgaaccccg ccctgccccg tcccccccga aggcagccgt ccccccgcgg 421 acagcccc //