LOCUS Exported 728 bp ds-DNA linear SYN 19-MAY-2021 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS pBabe-TetCMV-puro-mVenus-PA-Rac1-C450A SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 728) AUTHORS Wu YI, Frey D, Lungu OI, Jaehrig A, Schlichting I, Kuhlman B, Hahn KM TITLE A genetically encoded photoactivatable Rac controls the motility of living cells. JOURNAL Nature. 2009 Sep 3. 461(7260):104-8. PUBMED 19693014 REFERENCE 2 (bases 1 to 728) AUTHORS . TITLE Direct Submission JOURNAL Exported May 19, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..728 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 19..51 /codon_start=1 /product="HSV (herpes simplex virus) epitope tag" /label=HSV tag /translation="QPELAPEDPED" protein_bind 65..81 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter 114..613 /label=PGK promoter /note="mouse phosphoglycerate kinase 1 promoter" primer_bind complement(149..172) /label=MSCV-rev /note="Murine stem cell virus, reverse primer" primer_bind 546..565 /label=mPGK-F /note="Mouse PGK promoter, forward primer" primer_bind complement(636..655) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" ORIGIN 1 ctgtatacac gtgcaagcca gccagaactc gccccggaag accccgagga tctcgagtgt 61 ggaattgtga gcggataaca atttgtcgag ggcccctgca ggtcaattct accgggtagg 121 ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg 181 gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc 241 ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt 301 cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac 361 tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag 421 cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc 481 cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt cctccggagg 541 cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc 601 tccgggcctt tcgacctgca gccaataagc ttaccatgac cgagtacaag cccacggtgc 661 gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg 721 actacccc //