LOCUS       Exported                 767 bp ds-DNA     linear   SYN 19-MAY-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pBabe-TetCMV-puro-mVenus-PA-Rac1-C450A
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Wu YI, Frey D, Lungu OI, Jaehrig A, Schlichting I, Kuhlman B, Hahn 
            KM
  TITLE     A genetically encoded photoactivatable Rac controls the motility of 
            living cells.
  JOURNAL   Nature. 2009 Sep 3. 461(7260):104-8.
  PUBMED    19693014
REFERENCE   2  (bases 1 to 767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 19, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             499..531
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
     protein_bind    545..561
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(629..652)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 ttattctgcc atgttatggt agatggaaaa ccggtgaatc tgggcttatg ggatacagct
       61 ggactagaag attatgacag attacgcccc ctatcctatc cgcaaacaga tgtgttctta
      121 atttgctttt cccttgtgag tcctgcatca ttccaccacg tccgtgcaaa gtggtatcct
      181 gaggtgcggc accactgtcc caacactccc atcatcctag tgggaactaa acttgatctt
      241 agggatgata aagacacgat cgagaaactg aaggagaaga agctgactcc catcacctat
      301 ccgcagggtc tagccatggc taaggagatt ggtgctgtaa aatacctgga gtgctcggcg
      361 ctcacacagc gaggcctcaa gacagtgttt gacgaagcga tccgagcagt cctctgcccg
      421 cctcccgtga agaagaggaa gagaaaatgc ctgctgttgt aaaagcttgc ggccgcacag
      481 ctgtatacac gtgcaagcca gccagaactc gccccggaag accccgagga tctcgagtgt
      541 ggaattgtga gcggataaca atttgtcgag ggcccctgca ggtcaattct accgggtagg
      601 ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg
      661 gcgctacaca agtggcctct ggcctcgcac acattccaca ttccaccggt aggcgccaac
      721 ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcc
//
