LOCUS Exported 684 bp ds-DNA linear SYN 25-MAY-2021 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS OCT4 GFP puro Donor 1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 684) AUTHORS Hockemeyer D, Soldner F, Beard C, Gao Q, Mitalipova M, Dekelver RC, Katibah GE, Amora R, Boydston EA, Zeitler B, Meng X, Miller JC, Zhang L, Rebar EJ, Gregory PD, Urnov FD, Jaenisch R TITLE Efficient targeting of expressed and silent genes in human ESCs and iPSCs using zinc-finger nucleases. JOURNAL Nat Biotechnol. 2009 Aug 13. ():. PUBMED 19680244 REFERENCE 2 (bases 1 to 684) AUTHORS . TITLE Direct Submission JOURNAL Exported May 25, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..684 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 12..37 /label=SA /note="splice acceptor site" ORIGIN 1 gctcnccatg gcctgtggga ggaagagaag aggtcaggga tcctcctggg tgccaggtct 61 gggcagctgc aggtgaccac ttccccatca ggctgccctg tcatgaccac ctccccacac 121 cccaaccccg tcgaagctca cttgcctcct ccgggttttg ctccagcttc tccttctcca 181 gcttcacggc accaggggtg acggtgcagg gctccgggga ggccccatcg gagttgctct 241 ccaccccgac tcctgcttcg ccctcaggct gagaggtctc caagccgcct tggggcacta 301 gccccactcc aacctggggc ccacagtacg ccatcccccc acagaactca tacggcgggg 361 ggcatggggg aatcccccac acctcagagc ctggcccaac ccccggcccg attcctggcc 421 ctccaggagg gccttggaag cttagccagg tccgaggatc aacccagccc ggctccggcc 481 cccctggccc atcacctcca ccacctggag ggggcgagaa ggcgaaatcc gaagccaggt 541 gtcccgccat ggggaaggaa ggcgccccaa gccgggggcc tggtgaaatg agggcttgcg 601 aagggactac tcaacccctc tctccctccc cagtcccacc cactagcctt gacctctggc 661 cccgccccct ggatgggtgg agga //