LOCUS Exported 9991 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.. ACCESSION . VERSION . KEYWORDS TFORF3139 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9991) AUTHORS Joung J, Zhang F TITLE Multiplexed Overexpression of Regulatory Factors (MORF) Collection JOURNAL unpublished REFERENCE 2 (bases 1 to 9991) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9991 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 5..231 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" LTR 232..412 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 459..584 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1077..1310 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1495..1539 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" promoter 1770..1980 /label=SV40 promoter /note="SV40 early promoter" promoter 1792..1988 /label=SV40 promoter /note="SV40 early promoter" rep_origin 1839..1974 /label=SV40 ori /note="SV40 origin of replication" CDS 2020..2622 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS" primer_bind complement(2020..2039) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 2516..2536 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 2665..2782 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2920..4098 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 3151..4089 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 4046..4066 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 4118..4142 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(5664..5688) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 5696..5737 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 5848..6436 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(5901..5921) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(6319..6330) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 6508..6741 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 6819..6940 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(6856..6875) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 6910..6929 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 6980..7115 /label=SV40 ori /note="SV40 origin of replication" primer_bind complement(7135..7154) /label=T7 /note="T7 promoter, forward primer" promoter complement(7136..7154) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(7164..7181) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(7164..7180) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(7173..7195) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 7322..7777 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(7409..7428) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 7619..7640 /label=F1ori-F /note="F1 origin, forward primer" promoter 7803..7907 /gene="bla" /label=AmpR promoter CDS 7908..8768 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(8126..8145) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 8939..9527 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 9428..9447 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 9681..9698 /label=L4440 /note="L4440 vector, forward primer" protein_bind 9815..9836 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 9851..9881 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 9889..9905 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 9894..9916 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 9913..9929 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 9913..9929 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 9948..9968 /label=T3 /note="T3 promoter, forward primer" promoter 9950..9968 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" ORIGIN 1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa 61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac 121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa 181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc 241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag 301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct 361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc 421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc 481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt 541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga 601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat 661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt 721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag 781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa 841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa 901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg 961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta 1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga 1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg 1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg 1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc 1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg 1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat 1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac 1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat 1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca 1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga 1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg 1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa 1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca 1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca 1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg 1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct 1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg 2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc 2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc 2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg 2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg 2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa 2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc 2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga 2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac 2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga 2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag 2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc 2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca 2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg 2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc 2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg 2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc 3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc 3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt 3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct 3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac 3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct 3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc 3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt 3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg 3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt 3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga 3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc 3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg 3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg 3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt 3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt 3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg 3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt 4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt 4081 ccatttcagg tgtcgtgagg ctagcatcga ttgatcaaca agtttgtaca aaaaagcagg 4141 caccatgggt gaaactctgg gagattctcc tattgaccca gaaagcgatt ccttcactga 4201 tacactgtct gcaaacatat cacaagaaat gaccatggtt gacacagaga tgccattctg 4261 gcccaccaac tttgggatca gctccgtgga tctctccgta atggaagacc actcccactc 4321 ctttgatatc aagcccttca ctactgttga cttctccagc atttctactc cacattacga 4381 agacattcca ttcacaagaa cagatccagt ggttgcagat tacaagtatg acctgaaact 4441 tcaagagtac caaagtgcaa tcaaagtgga gcctgcatct ccaccttatt attctgagaa 4501 gactcagctc tacaataagc ctcatgaaga gccttccaac tccctcatgg caattgaatg 4561 tcgtgtctgt ggagataaag cttctggatt tcactatgga gttcatgctt gtgaaggatg 4621 caagggtttc ttccggagaa caatcagatt gaagcttatc tatgacagat gtgatcttaa 4681 ctgtcggatc cacaaaaaaa gtagaaataa atgtcagtac tgtcggtttc agaaatgcct 4741 tgcagtgggg atgtctcata atgccatcag gtttgggcgg atgccacagg ccgagaagga 4801 gaagctgttg gcggagatct ccagtgatat cgaccagctg aatccagagt ccgctgacct 4861 ccgggccctg gcaaaacatt tgtatgactc atacataaag tccttcccgc tgaccaaagc 4921 aaaggcgagg gcgatcttga caggaaagac aacagacaaa tcaccattcg ttatctatga 4981 catgaattcc ttaatgatgg gagaagataa aatcaagttc aaacacatca cccccctgca 5041 ggagcagagc aaagaggtgg ccatccgcat ctttcagggc tgccagtttc gctccgtgga 5101 ggctgtgcag gagatcacag agtatgccaa aagcattcct ggttttgtaa atcttgactt 5161 gaacgaccaa gtaactctcc tcaaatatgg agtccacgag atcatttaca caatgctggc 5221 ctccttgatg aataaagatg gggttctcat atccgagggc caaggcttca tgacaaggga 5281 gtttctaaag agcctgcgaa agccttttgg tgactttatg gagcccaagt ttgagtttgc 5341 tgtgaagttc aatgcactgg aattagatga cagcgacttg gcaatattta ttgctgtcat 5401 tattctcagt ggagaccgcc caggtttgct gaatgtgaag cccattgaag acattcaaga 5461 caacctgcta caagccctgg agctccagct gaagctgaac caccctgagt cctcacagct 5521 gtttgccaag ctgctccaga aaatgacaga cctcagacag attgtcacgg aacatgtgca 5581 gctactgcag gtgatcaaga agacggagac agacatgagt cttcacccgc tcctgcagga 5641 gatctacaag gacttgtact agaacccagc tttcttgtac aaagtggttg atatcggtaa 5701 gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgaacta gtccgtaact 5761 tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgagc aatggcgctc 5821 tcagacgtag tcacgcgtta agtcgacaat caacctctgg attacaaaat ttgtgaaaga 5881 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg 5941 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc 6001 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc 6061 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt 6121 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt 6181 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg 6241 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg 6301 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg 6361 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt 6421 tgggccgcct ccccgcgtcg actttaagac caatgactta caaggcagct gtagatctta 6481 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag 6541 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct 6601 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag 6661 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt 6721 cagtgtggaa aatctctagc agtacgtata gtagttcatg tcatcttatt attcagtatt 6781 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata 6841 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc 6901 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat 6961 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc 7021 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct 7081 attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat 7141 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac 7201 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat 7261 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg 7321 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc 7381 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc 7441 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt 7501 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg 7561 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt 7621 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta 7681 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt 7741 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg 7801 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga 7861 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac 7921 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc 7981 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca 8041 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc 8101 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg 8161 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac 8221 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca 8281 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg 8341 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac 8401 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg 8461 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat 8521 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg 8581 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg 8641 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc 8701 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc 8761 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt 8821 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt 8881 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt 8941 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag 9001 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca 9061 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca 9121 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg 9181 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg 9241 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct 9301 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga 9361 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc 9421 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg 9481 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg 9541 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt 9601 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc 9661 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac 9721 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc 9781 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg 9841 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat 9901 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc 9961 actaaaggga acaaaagctg gagctgcaag c //