LOCUS       Exported                 735 bp ds-DNA     linear   SYN 22-MAY-2021
DEFINITION  Lentiviral expression of SARS-CoV-2 protein; transient expression 
            and generate lentivirus.
ACCESSION   .
VERSION     .
KEYWORDS    pLVX-EF1alpha-SARS-CoV-2-orf9b-2xStrep-IRES-Puro
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Gordon DE, et al.
  TITLE     A SARS-CoV-2-Human Protein-Protein Interaction Map Reveals Drug 
            Targets and Potential Drug Repurposing
  JOURNAL   BioRxiv March 2020
REFERENCE   2  (bases 1 to 735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 22, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..735
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      18..27
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             333..356
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             393..416
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     primer_bind     complement(595..612)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 tttccggtga attcgccgcc accatggacc ccaaaatttc tgaaatgcac ccggcgcttc
       61 gcttggtgga cccccaaatc caactcgctg tcacgcggat ggaaaacgca gtaggacgcg
      121 accaaaataa tgtcggccca aaagtgtatc cgattatcct ccgccttggc agtcccctga
      181 gcctgaatat ggccagaaag actcttaaca gcctggagga caaagcgttt cagctgaccc
      241 ccattgcggt tcagatgact aagctggcga caactgaaga gttgcccgac gaatttgtgg
      301 tagtaaccgt aaagctcgaa ggcggcgggg gatggagcca tccacaattc gagaaaggcg
      361 gtggttcagg aggaggtagc gggggtggat catggtcaca tccgcagttt gaaaagtaag
      421 gatcccgccc ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag
      481 gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga
      541 gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg
      601 ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt
      661 gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca
      721 ggtgcctctg cggcc
//
