LOCUS Exported 10503 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pEGFP-C1-hAtg2A SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10503) AUTHORS Velikkakath AK, Nishimura T, Oita E, Ishihara N, Mizushima N TITLE Mammalian Atg2 proteins are essential for autophagosome formation and important for regulation of size and distribution of lipid droplets. JOURNAL Mol Biol Cell. 2012 Mar;23(5):896-909. Epub 2012 Jan 4. PUBMED 22219374 REFERENCE 2 (bases 1 to 10503) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10503 /organism="synthetic DNA construct" /mol_type="other DNA" rep_origin 1..589 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 490..509 /label=pBR322ori-F /note="pBR322 origin, forward primer" enhancer 764..1067 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 1068..1271 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 1222..1242 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" regulatory 1310..1319 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 1316..2032 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 1316..2032 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(1361..1382) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(1622..1641) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 1969..1990 /label=EGFP-C /note="EGFP, forward primer" polyA_signal 7994..8115 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(8031..8050) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 8085..8104 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(8122..8577) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(8259..8280) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 8471..8490 /label=F1ori-R /note="F1 origin, reverse primer" promoter 8604..8708 /gene="bla" /label=AmpR promoter promoter 8710..9067 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(8733..8753) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 8918..9053 /label=SV40 ori /note="SV40 origin of replication" primer_bind 8980..8999 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 9102..9896 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(9156..9175) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 9766..9785 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(10084..10103) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 10128..10175 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" ORIGIN 1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc 61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt 121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt 181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc 241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa 301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac 361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg 421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga 481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact 541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa 601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc 661 gttatcccct gattctgtgg ataaccgtat taccgccatg cattagttat taatagtaat 721 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg 781 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt 841 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac 901 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg 961 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact 1021 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt 1081 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc 1141 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc 1201 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata 1261 taagcagagc tggtttagtg aaccgtcaga tccgctagcg ctaccggtcg ccaccatggt 1321 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga 1381 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa 1441 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt 1501 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca 1561 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa 1621 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa 1681 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct 1741 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat 1801 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca 1861 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct 1921 gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct 1981 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtccggact 2041 cagatctatg tcacgatggc tgtggccatg gtcaaactgt gtgaaagagc gggtctgccg 2101 ctacttgctg caccactact taggtcactt cttccaagag cacctcagcc tggaccagct 2161 cagcctcgat ctgtacaagg gcagcgttgc cctgcgagac atccacctgg aaatctggtc 2221 tgtgaacgag gtgctggagt caatggagtc accgctggag ctggtggaag gcttcgtggg 2281 ctccatcgag gtggccgtgc cctgggctgc tctgctcacc gaccactgca cagtgcgcgt 2341 gtccggcctc cagctcacct tgcagccccg ccggggtcca gcgccagggg ctgccgactc 2401 acagagctgg gcctcatgca tgaccacaag cctgcagctg gcccaggagt gtctgcggga 2461 tgggctaccg gagccctctg agccaccaca gcccctggag gggctggaga tgtttgccca 2521 gaccattgag actgtgcttc ggaggatcaa agtgaccttc ctggacactg tcgtgagggt 2581 ggagcactct ccgggtgatg gggaacgtgg tgtggccgtc gaggtccgtg tgcagagact 2641 ggagtactgt gatgaggcag tgcgggaccc aagccaggcg ccgccggtgg atgtgcatca 2701 gccgcctgcc ttcctgcaca agctgctgca gctggcaggg gtccgcctgc actacgagga 2761 gctcccggca caggaagagc ctccagagcc ccccttgcag atcggcagct gctcagggta 2821 catggagctg atggtgaagt tgaagcaaaa tgaggccttc cctggcccca agttggaggt 2881 ggcgggacag ctgggctccc tgcacctgct cctgaccccg aggcagctcc agcaacttca 2941 ggaactgctc agcgccgtga gccttacaga ccacgagggc ctggctgaca agctgaacaa 3001 gagccgcccg ctaggtgccg aagacctgtg gctgattgag caggacctga accagcagct 3061 gcaggcaggg gcagtggctg agcccctcag cccagacccc cttaccaacc cccttctcaa 3121 cctggataac actgacctct tcttctccat ggctggcctc acaagcagtg tggcctcagc 3181 cctctctgag ctctccctct ccgatgtaga cctggcctcc tctgtgcgca gcgacatggc 3241 ctcccgccgg ctctctgccc aggcccaccc agctggcaag atggccccca accccctcct 3301 ggacaccatg cgccctgact cgctgctgaa gatgaccttg gggggtgtga ccctgacctt 3361 gcttcagacg tctgccccat cttccggacc acctgacctc gccacgcact ttttcaccga 3421 gtttgatgcc accaaggatg ggcccttcgg ttcccgagac ttccatcacc ttcgaccacg 3481 cttccagagg gcctgtccct gtagccatgt tcggctaacg ggcacagccg tgcagctgtc 3541 ctgggagctg cggacgggca gtcggggccg gcggacaacc agcatggaag tgcacttcgg 3601 gcagctggag gtgctggagt gtctgtggcc ccggggcacc tctgagcctg agtacacgga 3661 gatcctgacc tttcctggta cgctgggctc ccaggcctca gctcggccct gcgcccatct 3721 gcgccacaca cagatcctgc gccgtgtgcc taagagccga ccccggcgct cagttgcctg 3781 ccattgccac tcagaactgg ccctggacct ggccaacttc caggcggacg tggagctggg 3841 ggccctggac cggctggccg ccctactgcg cctggccacc gtacctgctg agcctccagc 3901 cggcctgctg acagagcccc tgccggcgat ggagcagcag acggtatttc ggctctctgc 3961 accccgggcc acgctgcggc tgcgcttccc cattgccgac ctgcggcctg agcgggaccc 4021 ctgggcgggc caggccgtgc gggctgagca gcttcggctg gagctgagtg agccccagtt 4081 ccggtcagag cttagcagtg ggcctggtcc cccagtcccc acccacctgg aactcacctg 4141 ctccgaccta catggtatct atgaagatgg agggaagcca cctgtccctt gcctgcgtgt 4201 ctccaaagcc ctggacccca agagcactgg gcgcaagtac ttcctgcccc aggtagtggt 4261 gactgtgaac ccccagtcca gcagcacaca gtgggaggtg gccccggaga agggagagga 4321 actggagctg tcagtggaga gtccctgtga gctgcgggaa cctgagccct cgcccttctc 4381 ctctaagagg accatgtatg agacagagga gatggtgatc cctggagacc ctgaggagat 4441 gaggacgttc cagagccgga ccctggcact gtcccgctgc agcctggaag tgatcctgcc 4501 cagtgtccac atctttctgc ccagcaagga ggtctacgag agcatctaca acaggatcaa 4561 caacgacctg ctcatgtggg agcctgcaga tctgcttccc acccccgacc ccgccgccca 4621 gccctcgggc ttccccggcc cctcaggctt ctggcacgac agctttaaga tgtgcaagtc 4681 agccttcaag ctggccaact gctttgatct caccccagac tcggactcgg atgacgagga 4741 tgcccacttc ttctcagtgg gggcatcagg tggcccacag gccgctgccc ctgaggcccc 4801 aagtcttcac ttgcagagca ccttctctac actggtgaca gtgctgaagg ggcggatcac 4861 agccctctgt gagaccaagg atgagggtgg gaagcggctg gaggctgtgc acggggagct 4921 ggtgctggac atggagcacg gtaccctctt cagcgtctcc cagtactgtg gccagccagg 4981 acttggctac ttctgtctgg aagctgaaaa ggcaacactc taccaccgag cggccgtgga 5041 tgactacccg ctgcccagtc acctggacct tcccagtttc gctcccccgg ctcagctggc 5101 cccaaccatc tacccatcgg aggaaggggt gaccgagcgg ggagcctcgg gccgcaaggg 5161 ccagggccgg ggaccccaca tgttgtccac tgctgtgcgc atccacctgg acccccacaa 5221 gaatgtgaag gagttcctgg tgacactgcg gttgcacaaa gccaccttgc gccactacat 5281 ggccctgccc gagcagagct ggcattccca gttgttggag ttcctagacg tgctggatga 5341 ccctgtgctg ggctacctgc ccccgacggt catcaccatc ctgcacacac acctgttctc 5401 ctgctctgtg gactataggc cactctacct cccagtgcgt gtcctcatca ccgcggagac 5461 cttcactctc tccagcaaca tcatcatgga cacctccacc ttcctgctca ggttcatcct 5521 cgatgactcc gccttgtacc tgtccgacaa gtgtgaggtg gagaccctgg acctgcggcg 5581 agattatgtc tgtgttttgg atgttgacct cttggaactt gtgattaaaa cctggaaagg 5641 gagcaccgag ggcaaactga gccagccact attcgagctg cgctgctcca acaatgtggt 5701 acacgtgcac agctgtgccg actcctgtgc cctgctggtc aacctgctcc agtacgtaat 5761 gagcacaggc gatctgcacc ccccaccccg gccccccagc cccacggaga tcgccggcca 5821 gaagctctcg gagagtcctg cctctctgcc ctcgtgcccc ccagtggaga cggccctcat 5881 caaccagcgt gacctggccg acgccctcct ggacaccgag cgcagcctac gggagctggc 5941 ccagccttca ggtggccacc tccctcaggc gtcgcccatc tccgtctacc tattcccagg 6001 tgaacggagt ggggccccac ccccttcacc acctgtcggg ggccctgctg gcagcttagg 6061 gtcatgctca gaggagaagg aagatgaaag ggaagaggag ggcgatggag acaccctgga 6121 cagtgatgag ttctgcatcc ttgatgctcc cggcctgggc atcccgcccc gagatgggga 6181 gcctgtggtg acacagctgc atcccggccc catcgttgtg agggacggtt acttctcacg 6241 gccgatcggc agcacggact tgctgcgggc acctgcccat ttcccagtgc ccagcactcg 6301 ggtggtgcta cgtgaggtct ccctcgtctg gcacctctat gggggccgag actttggccc 6361 ccaccccggc cacagggcaa gaactggcct ctcaggtccc aggagctccc cttcccgctg 6421 ctctggcccc aaccggcccc agaactcatg gcgcacgcag gggggcagcg ggcggcagca 6481 ccatgtcctc atggagatcc agctgagcaa ggtaagcttc cagcacgagg tgtacccagc 6541 ggagccagcc acaggccctg cggcccccag ccaggagctg gaggagcgac cgctgtcccg 6601 tcaggtgttc atcgtgcagg agctggaggt ccgagaccgg ctcgcctcct cccagatcaa 6661 caagttcctg tacctacaca cgagtgagcg gatgccgcga cgtgcccact ctaacatgct 6721 caccatcaaa gcgctgcatg tggcccccac taccaacctg ggtgggcctg agtgctgtct 6781 ccgcgtctcg ctgatgcccc tgcggctcaa tgtggaccag gatgccctct tcttcctcaa 6841 ggacttcttc actagtctgg tggccggcat caaccccgtg gtcccagggg agacctccgc 6901 tgaggctcgc cccgagactc gagcccagcc cagcagcccc ctggaagggc aggccgaagg 6961 cgtagagacc actggttcgc aggaggcccc aggaggtgga cacagcccct cccctcctga 7021 ccagcagccc atctacttca gagagttccg cttcacgtct gaggtcccca tctggctgga 7081 ttaccatggc aagcacgtca cgatggacca ggtgggcact tttgctggcc tcctcatcgg 7141 cctggcccaa ctcaactgct ccgagctgaa gctaaagcgg ctctgttgca ggcacgggct 7201 cctgggtgtg gacaaggtgc tgggctatgc cctcaacgag tggctgcagg acatccgcaa 7261 gaaccagctg cccggcctgc tgggaggcgt gggccccatg cactcggttg tccagctctt 7321 ccaagggttc cgggacctgc tgtggctgcc cattgagcag tacaggaagg atggccgcct 7381 catgcggggg ctgcagcgag gggctgcctc ctttggctca tccacagcct ctgccgccct 7441 ggaactcagc aaccggttgg tacaggctat ccaggccaca gctgagaccg tgtatgacat 7501 cctgtccccg gcagcccccg tctcccgctc cctgcaggat aagcgctctg cgcggaggct 7561 gcgcaggggc cagcagcctg ccgacctgcg ggagggtgtg gccaaggcct acgacacagt 7621 gcgagagggc atcttggata cagctcagac catctgtgac gtggcatcgc ggggccatga 7681 gcagaagggg ctgacgggcg ccgtgggggg cgtgatccgc cagctgcccc cgactgtggt 7741 gaagccgctc atcctggcca cggaggccac gtccagcctg ctcgggggca tgcgcaacca 7801 gattgtcccc gacgcccaca aggaccacgc cctcaagtgg cgctcggaca gtgcccaaga 7861 ctgaggatcc accggatcta gataactgat cataatcagc cataccacat ttgtagaggt 7921 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc 7981 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat 8041 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact 8101 catcaatgta tcttaacgcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa 8161 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata 8221 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac 8281 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc 8341 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa 8401 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg 8461 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg 8521 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag 8581 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt 8641 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa 8701 ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa 8761 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac 8821 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa 8881 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag 8941 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc 9001 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt 9061 ttgcaaagat cgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat 9121 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac 9181 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc 9241 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc 9301 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag 9361 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc 9421 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg 9481 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc 9541 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc 9601 cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga 9661 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca 9721 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg 9781 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg 9841 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg 9901 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga 9961 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg 10021 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccta gggggaggct 10081 aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag 10141 acagaataaa acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg 10201 ctggcactct gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc 10261 cttttcccca ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg 10321 ggcggcaggc cctgccatag cctcaggtta ctcatatata ctttagattg atttaaaact 10381 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat 10441 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc 10501 ttc //