LOCUS Exported 7111 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION expresses human Nox4 in mammalian cells. ACCESSION . VERSION . KEYWORDS pcDNA3.1-hNox4 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7111) AUTHORS Serrander L, Cartier L, Bedard K, Banfi B, Lardy B, Plastre O, Sienkiewicz A, Forro L, Schlegel W, Krause KH TITLE NOX4 activity is determined by mRNA levels and reveals a unique pattern of ROS generation. JOURNAL Biochem J. 2007 Aug 15;406(1):105-14. PUBMED 17501721 REFERENCE 2 (bases 1 to 7111) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7111 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(44..63) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 235..614 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 615..818 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 769..789 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 863..882 /label=T7 /note="T7 promoter, forward primer" promoter 863..881 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" regulatory 899..908 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" primer_bind complement(2702..2719) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 2708..2932 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 2978..3406 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(3065..3084) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 3275..3296 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(3415..3435) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 3420..3749 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 3600..3735 /label=SV40 ori /note="SV40 origin of replication" primer_bind 3662..3681 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 3816..4610 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(3870..3889) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 4480..4499 /label=Neo-F /note="Neomycin resistance gene, forward primer" polyA_signal 4784..4905 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(4821..4840) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 4875..4894 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(4954..4970) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(4954..4970) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(4967..4989) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 4978..4994 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(5002..5032) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 5047..5068 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(5185..5202) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(5356..5944) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(5436..5455) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(6115..6975) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 6738..6757 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(6976..7080) /gene="bla" /label=AmpR promoter ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 caccatggct gtgtcctgga ggagctggct cgccaacgaa ggggttaaac acctctgcct 961 gttcatctgg ctctccatga atgtcctgct tttctggaaa accttcttgc tgtataacca 1021 agggccagag tatcactacc tccaccagat gttggggcta ggattgtgtc taagcagagc 1081 ctcagcatct gttcttaacc tcaactgcag ccttatcctt ttacccatgt gccgaacact 1141 cttggcttac ctccgaggat cacagaaggt tccaagcagg agaaccagga gattgttgga 1201 taaaagcaga acattccata ttacctgtgg tgttactatc tgtattttct caggcgtgca 1261 tgtggctgcc catctggtga atgccctcaa cttctcagtg aattacagtg aagactttgt 1321 tgaactgaat gcagcaagat accgagatga ggatcctaga aaacttctct tcacaactgt 1381 tcctggcctg acaggggtct gcatggtggt ggtgctattc ctcatgatca cagcctctac 1441 atatgcaata agagtttcta actatgatat cttctggtat actcataacc tcttctttgt 1501 cttctacatg ctgctgacgt tgcatgtttc aggagggctg ctgaagtatc aaactaattt 1561 agatacccac cctcccggct gcatcagtct taaccgaacc agctctcaga atatttcctt 1621 accagagtat ttctcagaac attttcatga acctttccct gaaggatttt caaaaccggc 1681 agagtttacc cagcacaaat ttgtgaagat ttgtatggaa gagcccagat tccaagctaa 1741 ttttccacag acttggcttt ggatttctgg acctttgtgc ctgtactgtg ccgaaagact 1801 ttacaggtat atccggagca ataagccagt caccatcatt tcggtcataa gtcatccctc 1861 agatgtcatg gaaatccgaa tggtcaaaga aaattttaaa gcaagacctg gtcagtatat 1921 tactctacat tgtcccagtg tatctgcatt agaaaatcat ccatttaccc tcacaatgtg 1981 tccaactgaa accaaagcaa catttggggt tcatcttaaa atagtaggag actggacaga 2041 acgatttcga gatttactac tgcctccatc tagtcaagac tctgaaattc tgcccttcat 2101 tcaatctaga aattatccca agctgtatat tgatggtcct tttggaagtc catttgagga 2161 atcactgaac tatgaggtca gcctctgcgt ggctggaggc attggagtaa ctccatttgc 2221 atcaatactc aacaccctgt tggatgactg gaaaccatac aagcttagaa gactatactt 2281 tatttgggta tgcagagata tccagtcctt ccgttggttt gcagatttac tctgtatgtt 2341 gcataacaag ttttggcaag agaacagacc tgactatgtc aacatccagc tgtacctcag 2401 tcaaacagat gggatacaga agataattgg agaaaaatat catgcactga attcaagact 2461 gtttatagga cgtcctcggt ggaaactttt gtttgatgaa atagcaaaat ataacagagg 2521 aaaaacagtt ggtgttttct gttgtggacc caattcacta tccaagactc ttcataaact 2581 gagtaaccag aacaactcat atgggacaag atttgaatac aataaagagt ctttcagctg 2641 aaatcactag tgaattcgcg gccgctcgag tctagagggc ccgtttaaac ccgctgatca 2701 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc 2761 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg 2821 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg 2881 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag 2941 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta 3001 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg 3061 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa 3121 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc 3181 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt 3241 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca 3301 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc 3361 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg 3421 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca 3481 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa 3541 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca 3601 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt 3661 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag 3721 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg 3781 gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg 3841 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa 3901 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg 3961 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt 4021 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa 4081 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc 4141 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg 4201 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg 4261 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg 4321 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg 4381 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact 4441 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg 4501 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc 4561 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct 4621 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac 4681 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat 4741 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc 4801 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc 4861 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc 4921 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg 4981 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg 5041 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc 5101 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt 5161 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 5221 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 5281 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 5341 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 5401 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 5461 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 5521 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt 5581 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 5641 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 5701 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 5761 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct 5821 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 5881 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 5941 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 6001 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 6061 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 6121 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 6181 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 6241 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 6301 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 6361 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 6421 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc 6481 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag 6541 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt 6601 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac 6661 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg 6721 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 6781 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc 6841 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc 6901 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa 6961 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg 7021 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 7081 cacatttccc cgaaaagtgc cacctgacgt c //