LOCUS       Exported                5872 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Yeast Expression plasmid for SARS-CoV-2 nsp11.
ACCESSION   .
VERSION     .
KEYWORDS    pGBW-m4046488
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5872)
  TITLE     SARS-CoV-2 protein Yeast expression constructs, tagged, for protein 
            expression and/or purification
REFERENCE   2  (bases 1 to 5872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5872
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        10..417
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     CDS             467..484
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(1627..1645)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             1996..2799
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      2882..3864
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     terminator      3942..3971
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     rep_origin      3993..4580
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      4592..4619
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(4643..4834)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(4835..5695)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5458..5477
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5696..5798)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      5799..5830
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 gcggccgccc cacacaccat agcttcaaaa tgtttctact ccttttttac tcttccagat
       61 tttctcggac tccgcgcatc gccgtaccac ttcaaaacac ccaagcacag catactaaat
      121 ttcccctctt tcttcctcta gggtgtcgtt aattacccgt actaaaggtt tggaaaagaa
      181 aaaagagacc gcctcgtttc tttttcttcg tcgaaaaagg caataaaaat ttttatcacg
      241 tttctttttc ttgaaaattt ttttttttga tttttttctc tttcgatgac ctcccattga
      301 tatttaagtt aataaacggt cttcaatttc tcaagtttca gtttcatttt tcttgttcta
      361 ttacaacttt ttttacttct tgctcattag aaagaaagca tagcaatcta atctaagttt
      421 taattacaaa atggctgatg cacaatcgtt tttaaacggg tttgcgctcg tgccgagagg
      481 ttctaaaatc gaggaaggta agctagtcat ttggataaat ggagataagg gttacaacgg
      541 tttggcagaa gtaggcaaga aatttgaaaa ggacacaggt atcaaggtga cggttgaaca
      601 tccagacaag ttagaagaaa aattccctca agttgctgct actggtgatg gtcccgacat
      661 tattttctgg gcccacgatc gttttggtgg atatgctcag tcaggtttgc tggctgagat
      721 caccccagac aaggccttcc aagataaatt gtacccattc acttgggatg ctgtcagata
      781 caacggtaag cttatagcat atccaattgc tgttgaagcc ttatcgttga tctacaataa
      841 ggacttgtta cctaacccac caaagacatg ggaagaaatt ccagctttgg ataaagagct
      901 aaaggccaag ggaaaaagcg ctttgatgtt taacttacaa gaaccttatt tcacctggcc
      961 attaatcgct gctgacggtg gttacgcatt taaatacgaa aacggtaagt atgacattaa
     1021 ggatgtcggc gttgataatg ctggtgcaaa ggctggtttg accttcttgg ttgatttaat
     1081 aaagaataaa cacatgaacg ctgacactga ctactctatc gccgaagccg ctttcaacaa
     1141 gggtgaaact gctatgacca taaatggtcc atgggcctgg agtaacattg atacttctaa
     1201 agtaaactat ggtgtcactg ttttgcctac attcaaaggc caaccatcca agccctttgt
     1261 cggtgttcta tccgctggca tcaatgctgc ttctccaaac aaggagctag ctaaagaatt
     1321 tttggaaaat tacttgttaa ctgacgaagg tttggaggcc gttaacaagg acaagccatt
     1381 gggtgctgtc gctttgaaat cttacgaaga agaattggct aaggatccca gaattgccgc
     1441 taccatggag aacgcccaaa agggtgaaat tatgccaaac atcccacaaa tgtcagcctt
     1501 ctggtacgca gttaggactg ccgtgattaa tgctgcttcc ggtagacaaa ccgtcgacga
     1561 agcattaaaa gatgctcaaa cgtaaacagg ccccttttcc tttgtcgata tcatgtaatt
     1621 agttatgtca cgcttacatt cacgccctcc tcccacatcc gctctaaccg aaaaggaagg
     1681 agttagacaa cctgaagtct aggtccctat ttattttttt taatagttat gttagtatta
     1741 agaacgttat ttatatttca aatttttctt ttttttctgt acaaagcggc cgccgaacca
     1801 ctagtgcact gcagtacacg gtttccttga aatttttttg attcggtaat ctccgaacag
     1861 aaggaagaac gaaggaagga gcacagactt agattggtat atatacgcat atgtagtgtt
     1921 gaagaaacat gaaattgccc agtattctta acccaactgc acagaacaaa aacgtgcagg
     1981 aaacgaagat aaatcatgtc gaaagctaca tataaggaac gtgctgctac tcatcctagt
     2041 cctgttgctg ccaagctatt taatatcatg cacgaaaagc aaacaaactt gtgtgcttca
     2101 ttggatgttc gtaccaccaa ggaattactg gagttagttg aagcattagg tcccaaaatt
     2161 tgtttactaa aaacacatgt ggatatcttg actgattttt ccatggaggg cacagttaag
     2221 ccgctaaagg cattatccgc caagtacaat tttttactct tcgaggacag aaaatttgct
     2281 gacattggta atacagtcaa attgcagtac tctgcgggtg tatacagaat agcagaatgg
     2341 gcagacatta cgaatgcaca cggtgtggtg ggcccaggta ttgttagcgg tttgaagcag
     2401 gcggcagaag aagtaacaaa ggaacctaga ggccttttga tgttagcaga attgtcatgc
     2461 aagggctccc tatctactgg agaatatact aagggtactg ttgacattgc gaagagcgac
     2521 aaagattttg ttatcggctt tattgctcaa agagacatgg gtggaagaga tgaaggttac
     2581 gattggttga ttatgacacc cggtgtgggt ttagatgaca agggagatgc attgggtcaa
     2641 cagtatagaa ccgtggatga tgtggtttct acaggatctg acattattat tgttggaaga
     2701 ggactatttg caaagggaag ggatgctaag gtagagggtg aacgttacag aaaagcaggc
     2761 tgggaagcat atttgagaag atgcggccag caaaactaaa aaactgtatt ataagtaaat
     2821 gcatgtatac taaactcaca aattagagct tcaatttaat tatatcagtt attacccgag
     2881 taacgaagca tctgtgcttc attttgtaga acaaaaatgc aacgcgagag cgctaatttt
     2941 tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga aagcgctatt
     3001 ttaccaacga agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc gagagcgcta
     3061 atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgagagcg
     3121 ctattttacc aacaaagaat ctatacttct tttttgttct acaaaaatgc atcccgagag
     3181 cgctattttt ctaacaaagc atcttagatt actttttttc tcctttgtgc gctctataat
     3241 gcagtctctt gataactttt tgcactgtag gtccgttaag gttagaagaa ggctactttg
     3301 gtgtctattt tctcttccat aaaaaaagcc tgactccact tcccgcgttt actgattact
     3361 agcgaagctg cgggtgcatt ttttcaagat aaaggcatcc ccgattatat tctataccga
     3421 tgtggattgc gcatactttg tgaacagaaa gtgatagcgt tgatgattct tcattggtca
     3481 gaaaattatg aacggtttct tctattttgt ctctatatac tacgtatagg aaatgtttac
     3541 attttcgtat tgttttcgat tcactctatg aatagttctt actacaattt ttttgtctaa
     3601 agagtaatac tagagataaa cataaaaaat gtagaggtcg agtttagatg caagttcaag
     3661 gagcgaaagg tggatgggta ggttatatag ggatatagca cagagatata tagcaaagag
     3721 atacttttga gcaatgtttg tggaagcggt attcgcaata ttttagtagc tcgttacagt
     3781 ccggtgcgtt tttggttttt tgaaagtgcg tcatcagagc gcttttggtt ttcaaaagcg
     3841 ctctgaagtt cctatacttt ctagctagag aataggaact tcccgagcgg ccgcgtgtta
     3901 caagcttgac tccagcgtaa ctggactgca atcaactcac tggctcacct tcacgggtgg
     3961 gcctttcttc ggtagaaaat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     4021 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     4081 ctaccaactc tttttccgag gtaactggct tcagcagagc gcagatacca aatactgttc
     4141 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     4201 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     4261 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     4321 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     4381 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     4441 gcagggtcgg aacaggagag cgcacgaggg agcttccagg ggtaaacgcc tggtatcttt
     4501 atagtcctgt cgggtttcgc cacctctgac ttgagcatcg atttttgtga tgctcgtcag
     4561 gggggcggag cctatggaaa aacgccagca acgcagaaag gcccacccga aggtgagcca
     4621 ggtgattaca tttgggccct catcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc
     4681 agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat
     4741 ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg
     4801 ccatgttaag ggcggttttt tcctgtttgg tcacttacca atgcttaatc agtgaggcac
     4861 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     4921 taactacgat gcgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     4981 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccgggagg gccgagcgca
     5041 gaagtgatcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     5101 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     5161 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     5221 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     5281 ttgccagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     5341 ctcttactgt catgccatcc gtgagatgct tttctgtgac tggtgagtac tcaaccaagt
     5401 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     5461 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     5521 gtaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     5581 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     5641 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcattttag
     5701 cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt
     5761 cattatggtg aaagttggaa cctcttacgt gccgatcaag tcaaaagcct ccggtcggag
     5821 gcttttgact ttctgctatg gaggtcaggt atgatttaaa tggtcagtta ga
//
