LOCUS       Exported                1770 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Bacterial Expression plasmid for SARS-CoV-2 nsp11.
ACCESSION   .
VERSION     .
KEYWORDS    pGBW-m4046829
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1770)
  TITLE     SARS-CoV-2 protein E. coli expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     20..39
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        20..38
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    39..63
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             78..100
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             144..164
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             165..191
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     rep_origin      308..896
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     797..816
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1002..1661
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1068..1087)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1732..1766
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 gatctcgatc ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat
       61 tcccctctag aaataatttt gtttaacttt aagaaggaga ctcttctatg gctgatgcac
      121 aatcgttttt aaacgggttt gcggagaacc tgtactttca aagctacccg tatgacgttc
      181 ctgattatgc ctaaccggct tatcggtcag tttcacctga tttacgtaaa aacccgcttc
      241 ggcgggtttt tgcttttgga ggggcagaaa gatgaatgac tgtccacgac gctataccca
      301 aaagaaattg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
      361 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
      421 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
      481 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
      541 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
      601 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
      661 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
      721 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
      781 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
      841 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaattga
      901 tcgggcacgt aagaggttcc aactttcacc ataatgaaat aagatcacta ccgggcgtat
      961 tttttgagtt atcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg
     1021 gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt
     1081 cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga
     1141 ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga
     1201 tgaatgctca tccggaattt cgtatggcaa tgaaagacgg tgagctggtg atatgggata
     1261 gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga
     1321 gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt
     1381 acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtttcag
     1441 ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct
     1501 tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc
     1561 tggcgattca ggttcatcat gccgtttgtg atggcttcca tgtcggcaga atgcttaatg
     1621 aattacaaca gtactgcgat gagtggcagg gcggggcgta atttgatatc gagctcgctt
     1681 ggactcctgt tgatagatcc agtaatgacc tcagaactcc atctggattt gttcagaacg
     1741 ctcggttgcc gccgggcgtt ttttattggt
//
