LOCUS Exported 11630 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Lentiviral vector to express mutant N terminal HTT gene with partial long 3'UTR in mammalian cells (encodes Myc-tagged mutant huntingtin exon1 fragment with 145 repeats of Q). ACCESSION . VERSION . KEYWORDS pUltra-Exon1Q145-Myc-B SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11630) AUTHORS Xu H, An JJ, Xu B TITLE Distinct cellular toxicity of two mutant huntingtin mRNA variants due to translation regulation. JOURNAL PLoS One. 2017 May 11;12(5):e0177610. doi: 10.1371/journal.pone.0177610. eCollection 2017. PUBMED 28494017 REFERENCE 2 (bases 1 to 11630) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11630 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 210..327 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 392..1603 /label=UbC promoter /note="human ubiquitin C promoter" primer_bind 1440..1459 /label=hUBCpro-F /note="Human Ubiquitin C (UbC) promoter, forward primer" CDS 2339..2368 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" CDS 2384..2401 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" CDS 2429..2482 /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /label=T2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" misc_feature 5847..6435 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(5900..5920) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(6318..6329) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 6526..6759 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 6831..6952 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(6868..6887) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 6922..6941 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 6992..7127 /label=SV40 ori /note="SV40 origin of replication" primer_bind 7054..7073 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind complement(7147..7166) /label=T7 /note="T7 promoter, forward primer" promoter complement(7148..7166) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(7176..7193) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(7176..7192) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(7185..7207) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 7334..7789 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(7421..7440) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 7631..7652 /label=F1ori-F /note="F1 origin, forward primer" promoter 7815..7919 /gene="bla" /label=AmpR promoter CDS 7920..8780 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(8138..8157) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 8951..9539 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 9440..9459 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 9693..9710 /label=L4440 /note="L4440 vector, forward primer" protein_bind 9827..9848 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 9863..9893 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 9901..9917 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 9906..9928 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 9925..9941 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 9925..9941 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 9960..9980 /label=T3 /note="T3 promoter, forward primer" promoter 9962..9980 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" promoter 10008..10234 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" LTR 10235..10415 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 10462..10587 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 11080..11313 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 11498..11542 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" ORIGIN 1 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga 61 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag 121 agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt gcgccaattc 181 tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac 241 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa 301 aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt 361 tggttaatta acccgtgtcg gctccagatc tggcctccgc gccgggtttt ggcgcctccc 421 gcgggcgccc ccctcctcac ggcgagcgct gccacgtcag acgaagggcg cagcgagcgt 481 cctgatcctt ccgcccggac gctcaggaca gcggcccgct gctcataaga ctcggcctta 541 gaaccccagt atcagcagaa ggacatttta ggacgggact tgggtgactc tagggcactg 601 gttttctttc cagagagcgg aacaggcgag gaaaagtagt cccttctcgg cgattctgcg 661 gagggatctc cgtggggcgg tgaacgccga tgattatata aggacgcgcc gggtgtggca 721 cagctagttc cgtcgcagcc gggatttggg tcgcggttct tgtttgtgga tcgctgtgat 781 cgtcacttgg tgagtagcgg gctgctgggc tggccggggc tttcgtggcc gccgggccgc 841 tcggtgggac ggaagcgtgt ggagagaccg ccaagggctg tagtctgggt ccgcgagcaa 901 ggttgccctg aactgggggt tggggggagc gcagcaaaat ggcggctgtt cccgagtctt 961 gaatggaaga cgcttgtgag gcgggctgtg aggtcgttga aacaaggtgg ggggcatggt 1021 gggcggcaag aacccaaggt cttgaggcct tcgctaatgc gggaaagctc ttattcgggt 1081 gagatgggct ggggcaccat ctggggaccc tgacgtgaag tttgtcactg actggagaac 1141 tcggtttgtc gtctgttgcg ggggcggcag ttatggcggt gccgttgggc agtgcacccg 1201 tacctttggg agcgcgcgcc ctcgtcgtgt cgtgacgtca cccgttctgt tggcttataa 1261 tgcagggtgg ggccacctgc cggtaggtgt gcggtaggct tttctccgtc gcaggacgca 1321 gggttcgggc ctagggtagg ctctcctgaa tcgacaggcg ccggacctct ggtgagggga 1381 gggataagtg aggcgtcagt ttctttggtc ggttttatgt acctatcttc ttaagtagct 1441 gaagctccgg ttttgaacta tgcgctcggg gttggcgagt gtgttttgtg aagtttttta 1501 ggcacctttt gaaatgtaat catttgggtc aatatgtaat tttcagtgtt agactagtaa 1561 attgtccgct aaattctggc cgtttttggc ttttttgtta gacgaagctt gggctgcagg 1621 tccgatccac cgggatccac tagtccagtg tggtggaatt ctgcagataa acaccaccat 1681 ggcgaccctg gaaaagctga tgaaggcctt cgagtccctc aagtccttcc aacaacagca 1741 gcaacaacaa cagcaacagc agcaacagca acagcagcaa cagcagcaac agcagcaaca 1801 acagcaacag caacaacaac agcaacagca gcaacagcaa cagcaacagc agcagcaaca 1861 gcagcaacag cagcaacaac agcagcaaca gcaacagcag caacagcaac agcagcaaca 1921 acagcagcag cagcaacagc aacagcagca acagcagcag cagcagcagc aacaacagca 1981 acagcagcaa cagcagcaac agcagcagca gcagcaacaa caacagcagc aacaacagca 2041 acagcagcaa caacagcaac agcaacagca acagcaacag cagcagcagc aacagcaaca 2101 gcagcagcaa caacaacagc agcaacagca acaacagcaa caacaacagc aacagcaaca 2161 gcaaccgcca ccgccgccgc cgccgccgcc gcctcctcag cttcctcagc cgccgccgca 2221 ggcacagccg ctgctgcctc agccgcagcc gcccccgccg ccgcccccgc cgccacccgg 2281 cccggctgtg gctgaggagc cgctgcaccg accagcggcc gctcgaggtc acccattcga 2341 acaaaaactc atctcagaag aggatctgaa tatgcatacc ggtcatcatc accatcacca 2401 ttgagtttct agaggatccg gctccggcga gggcagggga agtctactaa catgcgggga 2461 cgtggaggaa aatcccggcc cagctagcgt gtgatcagaa ttcatttaat tatatcagta 2521 aagagattaa ttttaacgta actctttcta tgcccgtgta aagtatgtga atcgcaaggc 2581 ctgtgctgca tgcgacagcg tccggggtgg tggacagggc ccccggccac gctccctctc 2641 ctgtagccac tggcatagcc ctcctgagca cccgctgaca tttccgttgt acatgttcct 2701 gtttatgcat tcacaaggtg actgggatgt agagaggcgt tagtgggcag gtggccacag 2761 caggactgag gacaggcccc cattatccta ggggtgcgct cacctgcagc ccctcctcct 2821 cgggcacaga cgactgtcgt tctccaccca ccagtcaggg acagcagcct ccctgtcact 2881 cagctgagaa ggccagccct ccctggctgt gagcagcctc cactgtgtcc agagacatgg 2941 gcctcccact cctgttcctt gctagccctg gggtggcgtc tgcctaggag ctggctggca 3001 ggtgttggga cctgctgctc catggatgca tgccctaaga gtgtcactga gctgtgtttt 3061 gtctgagcct ctctcggtca acagcaaagc ttggtgtctt ggcactgtta gtgacagagc 3121 ccagcatccc ttctgccccc gttccagctg acatcttgca cggtgacccc ttttagtcag 3181 gagagtgcag atctgtgctc atcggagact gccccacggc cctgtcagag ccgccactcc 3241 tatccccagg ccaggtccct ggaccagcct cctgtttgca ggcccagagg agccaagtca 3301 ttaaaatgga ggtggattct ggatggccgg gctgctgctg atgtaggagc tggatttggg 3361 agctctgctt gctgactggc tgtgagacga ggcaggggct ctgcttcctc agccctagag 3421 gcgagccagg caaggttggc gactgtcatg tggcttggtt tggtcatgcc cgtcgatgtt 3481 ttgggtattg aatgtggtaa gtggaggaaa cgttggaact ctgtgcaggt gctgccttga 3541 gacccccaag cttccacctg tccctctcct atgtggcagc tggggagcag ctgagatgtg 3601 gacttgtatg ctgcccacat acgtgagggg gagctgaaag ggagcccctc ctctgagcag 3661 cctctgccag gcctgtatga ggcttttccc accagctccc aacagaggcc tcccccagcc 3721 aggaccacct cgtcctcgtg gcggggcagc aggagcggta gaaaggggtc cgatgtttga 3781 ggaggccctt aagggaagct actgaattat aacacgtaag aaaatcacca ttcttccgta 3841 ttggttgggg gctcctgttt ctcatcctag ctttttcctg gaaagcccgc tagaaggttt 3901 gggaacgagg ggaaagttct cagaactgtt ggctgctccc cacccgcctc ccgcctcccc 3961 cgcaggttat gtcagcagct ctgagacagc agtatcacag gccagatgtt gttcctggct 4021 agatgtttac atttgtaaga aataacactg tgaatgtaaa acagagccat tcccttggaa 4081 tgcatatcgc tgggctcaac atagagtttg tcttcctctt gtttacgacg tgatctaaac 4141 cagtccttag caaggggctc agaacacccc gctctggcag taggtgtccc ccacccccaa 4201 agacctgcct gtgtgctccg gagatgaata tgagctcatt agtaaaaatg acttcaccca 4261 cgcatataca taaagtatcc atgcatgtgc atatagacac atctataatt ttacacacac 4321 acctctcaag acggagatgc atggcctcta agagtgcccg tgtcggttct tcctggaagt 4381 tgactttcct tagacccgcc aggtcaagtt agccgcgtga cggacatcca ggcgtgggac 4441 gtggtcaggg cagggctcat tcattaccca ctaggatccc actggcgaag atggtctcca 4501 tatcagctct ctgcagaagg gaggaagact ttatcatgtt cctaaaaatc tgtggcaagc 4561 acccatcgta ttatccaaat tttgttgcaa atgtgattaa tttggttgtc aagttttggg 4621 ggtgggctgt ggggagattg cttttgtttt cctgctggta atatcgggaa agattttaat 4681 gaaaccaggg tagaattgtt tggcaatgca ctgaagcgtg tttctttccc aaaatgtgcc 4741 tcccttccgc tgcgggccca gctgtgtcta tgtaggtgat gtttccagct gccaagtgct 4801 ctttgttact gtccaccctc atttctgcca gcgcatgtgt cctttcaagg ggaaaatgtg 4861 aagctgaacc ccctccagac acccagaatg tagcatctga gaaggccctg tgccctaaag 4921 gacacccctc gcccccatct tcatggaggg ggtcatttca gagccctcgg agccaatgaa 4981 cagctcctcc tcttggagct gagatgagcc ccacgtggag ctcgggacgg atagtagaca 5041 gcaataactc ggtgtgtggc cgcctggcag gtggaacttc ctcccgttgc ggggtggagt 5101 gaggttagtt ctgtgtgtct ggtgggtgga gtcaggcttc tcttgctacc tgtgagcatc 5161 cttcccagca gacatcctca tcgggctttg tccctccccc gcttcctccc tctgcgggga 5221 ggacccggga ccacagctgc tggccagggt agacttggag ctgtcctcca gaggggtcac 5281 gtgtaggagt gagaagaagg aagatcttga gagctgctga gggaccttgg agagctcagg 5341 atggctcaga cgaggacact cgcttgccgg gcctgggcct cctgggaagg agggagctgc 5401 tcagaatgcc gcatgacaac tgaaggcaac ctggaaggtt caggggccgc tcttccccca 5461 tgtgcctgtc acgctctgat gcagtcaaag gaacgccttc ccctcagttg tttctaagag 5521 cagagtctcc cgctgcaatc tgggtggtaa ctgccagcct tggaggatcg tggccaacgt 5581 ggacctgcct acggagggtg ggctctgacc caagtggggc ctccttgtcc aggtctcact 5641 gctttgcacc gtggtcagag ggactgtcag ctgagcttga gctcccctgg agccagcagg 5701 gctgtgatgg gcgagtcccg gagccccacc cagacctgaa tgcttctgag agcaaaggga 5761 aggactgacg agagatgtat atttaatttt ttaactgctg caaacattgt acatccaaga 5821 attcgttccg gagtcgtcga ctcgacaatc aacctctgga ttacaaaatt tgtgaaagat 5881 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc 5941 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct 6001 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca 6061 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt 6121 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg 6181 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga 6241 agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt 6301 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc 6361 cggctctgcg gcctcttccg catcttcgcc ttcgccctca gacgagtcgg atctcccttt 6421 gggccgcctc cccgcctgga attaattcga gctcggtacc tttaagacca atgacttaca 6481 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctacgta 6541 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga 6601 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct 6661 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat 6721 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta 6781 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta 6841 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat 6901 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct 6961 ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc 7021 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc 7081 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc 7141 aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt 7201 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc 7261 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg 7321 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg 7381 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct 7441 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta 7501 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt 7561 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg 7621 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat 7681 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt 7741 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt 7801 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt 7861 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta 7921 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg 7981 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac 8041 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg 8101 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc 8161 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg 8221 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat 8281 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg 8341 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg 8401 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc 8461 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt 8521 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct 8581 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc 8641 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca 8701 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct 8761 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt 8821 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga 8881 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca 8941 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac 9001 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg 9061 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag 9121 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac 9181 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt 9241 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg 9301 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc 9361 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc 9421 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc 9481 acctctcact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa 9541 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt 9601 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg 9661 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag 9721 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 9781 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 9841 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 9901 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg 9961 caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc 10021 aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga 10081 gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg 10141 aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga 10201 tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg 10261 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc 10321 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct 10381 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa 10441 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg 10501 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag 10561 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg 10621 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg 10681 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc 10741 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga 10801 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac 10861 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag 10921 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga 10981 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa 11041 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt 11101 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag 11161 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca 11221 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc 11281 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact 11341 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat 11401 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat 11461 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga 11521 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat 11581 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt //