LOCUS Exported 9211 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Gateway-compatible Entry vector, with insert of NSP3 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. ACCESSION . VERSION . KEYWORDS pDONR207 SARS-CoV-2 NSP3 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9211) AUTHORS Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale M, Raught B, Jacob Y, Roth FP TITLE A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions. JOURNAL G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 10.1534/g3.120.401554. PUBMED 32763951 REFERENCE 2 (bases 1 to 9211) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9211 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 56..83 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 175..261 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" protein_bind 314..413 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(6255..6354) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(6466..6485) /label=pENTR-R /note="pENTR vectors, reverse primer" primer_bind complement(6544..6563) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" CDS complement(7137..7670) /codon_start=1 /gene="aacC1" /product="gentamycin acetyltransferase" /label=GmR /note="confers resistance to gentamycin" /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR EEVMHFDIDPSTAT" promoter complement(7859..7887) /gene="intI1 (promoter lies within the coding sequence)" /label=Pc promoter /note="class 1 integron promoter" rep_origin 8544..9132 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 9033..9052 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctgattctgt ggataaccgt attaccgcta gccaggaaga gtttgtagaa acgcaaaaag 61 gccatccgtc aggatggcct tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc 121 tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg gcggatttgt 181 cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtcttccga 241 ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcgtta acgctagcat 301 ggatctcggg ccccaaataa tgattttatt ttgactgata gtgacctgtt cgttgcaaca 361 aattgatgag caatgctttt ttataatgcc aactttgtac aaaaaagttg gcatggcccc 421 taccaaggtg accttcggag acgacaccgt gatcgaggtg cagggttaca agagcgtgaa 481 catcaccttc gagctggacg aacgcatcga caaggtgctg aacgaaaagt gctccgctta 541 caccgtggag ctgggcaccg aggtgaacga attcgcctgc gtggtggccg acgctgtgat 601 caagaccctg cagccagtgt ccgaactgct gacccctctg ggtatcgacc tggacgagtg 661 gtctatggct acctactacc tgttcgacga gagcggcgaa ttcaagctgg cctctcacat 721 gtactgcagc ttctaccctc ccgacgaaga cgaggaagag ggtgactgcg aagaggaaga 781 gttcgagcct tccactcagt acgagtacgg caccgaagac gactaccagg gcaagcctct 841 ggaattcgga gccacctctg ccgctctgca gcctgaagag gaacaggagg aagactggct 901 ggacgacgac tcccagcaga ccgtgggtca gcaggacggc tctgaagaca accagaccac 961 caccatccag accatcgtgg aggtgcagcc tcagctggag atggaactga ccccagtggt 1021 gcagaccatc gaggtgaaca gcttctccgg ctacctgaag ctgaccgaca acgtgtacat 1081 caagaacgct gacatcgtgg aggaagccaa gaaggtgaaa ccaaccgtgg tggtgaacgc 1141 cgctaacgtg tacctgaagc acggcggagg tgtggctgga gctctgaaca aggctaccaa 1201 caacgccatg caggtggaat ccgacgacta catcgctacc aacggtcctc tgaaggtggg 1261 cggatcctgc gtgctgtctg gccacaacct ggccaagcac tgcctgcacg tggtgggacc 1321 aaacgtgaac aagggagagg acatccagct gctgaagagc gcctacgaaa acttcaacca 1381 gcacgaggtg ctgctggctc cactgctgtc tgccggaatc ttcggtgctg atcccatcca 1441 cagcctgcgc gtgtgcgtgg acaccgtgag gaccaacgtg tacctggccg tgttcgacaa 1501 gaacctgtac gacaagctgg tgagctcctt cctggagatg aagtccgaaa agcaggtgga 1561 gcagaagatc gccgagatcc ccaaggagga agtgaagcct ttcatcaccg aatctaagcc 1621 cagcgtggag cagagaaagc aggacgacaa gaagatcaag gcctgcgtgg aggaagtgac 1681 caccaccctg gaggaaacca agttcctgac cgagaacctg ctgctgtaca tcgacatcaa 1741 cggcaacctg caccctgact ccgctaccct ggtgtctgac atcgacatca ccttcctgaa 1801 gaaggacgcc ccctacatcg tgggcgacgt ggtgcaggaa ggagtgctga ccgctgtggt 1861 catccctacc aagaaggccg gtggcaccac cgagatgctg gccaaggctc tgcgtaaggt 1921 gccaaccgac aactacatca ccacctaccc cggtcagggc ctgaacggtt acaccgtgga 1981 ggaagctaag accgtgctga agaagtgcaa gtctgccttc tacatcctgc cctccatcat 2041 ctctaacgag aagcaggaaa tcctgggcac cgtgagctgg aacctgcgcg aaatgctggc 2101 ccacgctgag gaaaccagga agctgatgcc cgtgtgcgtg gagaccaagg ccatcgtgag 2161 caccatccag agaaagtaca agggcatcaa gatccaggag ggtgtggtgg actacggcgc 2221 tcgtttctac ttctacacct ccaagaccac cgtggcctct ctgatcaaca ccctgaacga 2281 cctgaacgag accctggtga ccatgccact gggatacgtg acccacggtc tgaacctgga 2341 ggaagccgct agatacatgc gttccctgaa ggtgcctgct accgtgagcg tgagcagccc 2401 tgacgctgtg accgcctaca acggatacct gacctcctct agcaagaccc ctgaggaaca 2461 cttcatcgaa accatcagcc tggccggttc ctacaaggac tggtcttaca gcggacagtc 2521 cacccagctg ggcatcgagt tcctgaagcg cggagacaag agcgtgtact acacctccaa 2581 cccaaccacc ttccacctgg acggtgaagt gatcaccttc gacaacctga agaccctgct 2641 gtctctgcgc gaggtgagga ccatcaaggt gttcaccacc gtggacaaca tcaacctgca 2701 cacccaggtg gtggacatga gcatgaccta cggacagcag ttcggtccta cctacctgga 2761 cggagctgac gtgaccaaga tcaagccaca caactcccac gagggcaaga ccttctacgt 2821 gctgcctaac gatgacaccc tgagagtgga ggctttcgaa tactaccaca ccaccgaccc 2881 atccttcctg ggcaggtaca tgtctgccct gaaccacacc aagaagtgga agtaccccca 2941 ggtgaacgga ctgaccagca tcaagtgggc cgacaacaac tgctacctgg ccaccgctct 3001 gctgaccctg cagcagatcg aactgaagtt caacccaccc gctctgcagg acgcttacta 3061 cagagcccgt gctggcgagg ccgctaactt ctgcgctctg atcctggcct actgcaacaa 3121 gaccgtgggc gaactgggag acgtgagaga gaccatgagc tacctgttcc agcacgccaa 3181 cctggactcc tgcaagcgtg tgctgaacgt ggtgtgcaag acctgcggcc agcagcagac 3241 caccctgaag ggagtggaag ctgtgatgta catgggtacc ctgtcctacg agcagttcaa 3301 gaagggcgtg cagatccctt gcacctgcgg aaagcaggcc accaagtacc tggtgcagca 3361 ggaaagccca ttcgtgatga tgtccgctcc tccagcccag tacgaactga agcacggcac 3421 cttcacctgc gcttccgagt acaccggcaa ctaccagtgc ggacactaca agcacatcac 3481 ctctaaggag accctgtact gcatcgacgg agccctgctg accaagtcct ctgaatacaa 3541 gggtcccatc accgacgtgt tctacaagga gaacagctac accaccacca tcaagcccgt 3601 gacctacaag ctggacggcg tggtgtgcac cgaaatcgac cctaagctgg acaactacta 3661 caagaaggac aactcttact tcaccgagca gcctatcgac ctggtgccaa accagcccta 3721 ccctaacgcc agcttcgaca acttcaagtt cgtgtgcgac aacatcaagt tcgctgacga 3781 cctgaaccag ctgaccggat acaagaagcc tgcctctaga gagctgaagg tgaccttctt 3841 cccagacctg aacggcgacg tggtggctat cgactacaag cactacaccc caagcttcaa 3901 gaagggagcc aagctgctgc acaagcccat cgtgtggcac gtgaacaacg ctaccaacaa 3961 ggccacctac aagcctaaca cctggtgcat ccgttgcctg tggtctacca agccagtgga 4021 aacctccaac tctttcgacg tgctgaagag cgaggacgct cagggcatgg acaacctggc 4081 ctgcgaagac ctgaagcccg tgagcgagga agtggtggag aacccaacca tccagaagga 4141 cgtgctggaa tgcaacgtga agaccaccga ggtggtggga gacatcatcc tgaagccagc 4201 taacaacagc ctgaagatca ccgaggaagt gggtcacacc gacctgatgg ccgcttacgt 4261 ggacaacagc tccctgacca tcaagaagcc caacgagctg agcagggtgc tgggtctgaa 4321 gaccctggct actcacggtc tggctgctgt gaactctgtg ccatgggaca ccatcgctaa 4381 ctacgccaag cctttcctga acaaggtggt gtccaccacc accaacatcg tgaccagatg 4441 tctgaaccgc gtgtgcacca actacatgcc ctacttcttc accctgctgc tgcagctgtg 4501 caccttcacc agaagcacca actcccgtat caaggctagc atgcctacca ccatcgccaa 4561 gaacaccgtg aagagcgtgg gaaagttctg cctggaggct tccttcaact acctgaagtc 4621 tccaaacttc agcaagctga tcaacatcat catctggttc ctgctgctct ccgtgtgcct 4681 gggttccctg atctactcta ccgccgctct gggagtgctg atgtctaacc tgggtatgcc 4741 tagctactgc actggttaca gagagggata cctgaacagc accaacgtga ccatcgccac 4801 ctactgcacc ggcagcatcc catgctccgt gtgcctgtct ggactggaca gcctggacac 4861 ctacccctcc ctggaaacca tccagatcac catctctagc ttcaagtggg acctgaccgc 4921 cttcggtctg gtggctgagt ggttcctggc ctacatcctg ttcaccaggt tcttctacgt 4981 gctgggcctg gccgctatca tgcagctgtt cttctcctac ttcgctgtgc acttcatcag 5041 caactcctgg ctgatgtggc tgatcatcaa cctggtgcag atggctccca tctctgccat 5101 ggtgcgcatg tacatcttct tcgccagctt ctactacgtg tggaagtcct acgtgcacgt 5161 ggtggacggt tgcaactcct ctacctgcat gatgtgctac aagcgcaaca gggctaccag 5221 ggtggagtgc accaccatcg tgaacggcgt gcgcaggtcc ttctacgtgt acgccaacgg 5281 cggcaaggga ttctgcaagc tgcacaactg gaactgcgtg aactgcgaca ccttctgcgc 5341 tggctccacc ttcatctctg acgaggtggc cagagacctg agcctgcagt tcaagcgtcc 5401 aatcaacccc accgaccaga gctcctacat cgtggactct gtgaccgtga agaacggtag 5461 catccacctg tacttcgaca aggctggcca gaagacctac gagcgccact ctctgagcca 5521 cttcgtgaac ctggacaacc tgagggccaa caacaccaag ggcagcctgc ctatcaacgt 5581 gatcgtgttc gacggaaagt ccaagtgcga ggaatctagc gccaagtccg ccagcgtgta 5641 ctacagccag ctgatgtgcc agccaatcct gctgctggac caggctctgg tgtctgacgt 5701 gggagacagc gccgaagtgg ccgtgaagat gttcgacgcc tacgtgaaca ccttctcctc 5761 taccttcaac gtgcctatgg agaagctgaa gaccctggtg gctactgctg aggctgaact 5821 ggctaagaac gtgtccctgg acaacgtgct gagcaccttc atctccgccg ctaggcaggg 5881 tttcgtggac tctgacgtgg aaaccaaaga cgtggtggag tgcctgaagc tgtctcacca 5941 gagcgacatc gaagtgaccg gcgactcctg caacaactac atgctgacct acaacaaggt 6001 ggagaacatg accccaagag acctgggagc ttgcatcgac tgctccgccc gtcacatcaa 6061 cgctcaggtg gccaagtctc acaacatcgc cctgatctgg aacgtgaagg acttcatgag 6121 cctgtccgag cagctgcgca agcagatcag gagcgccgct aagaagaaca acctgccctt 6181 caagctgacc tgcgctacca ccagacaggt ggtgaacgtg gtgaccacca agatcgccct 6241 gaagggcgga taatacccaa ctttcttgta caaagttggc attataagaa agcattgctt 6301 atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgccatcc 6361 agctgcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata 6421 tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttatga 6481 gccatattca acgggaaacg tcgaggccgc gattaaattc caacatggat gctgatttat 6541 atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt 6601 atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg 6661 atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct cttccgacca 6721 tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg atccccggaa 6781 aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc 6841 tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacagcg 6901 atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga 6961 gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata 7021 aacttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc 7081 ttatttttga cgaggggaaa ttaggccggg aagccgatct cggcttgaac gaattgttag 7141 gtggcggtac ttgggtcgat atcaaagtgc atcacttctt cccgtatgcc caactttgta 7201 tagagagcca ctgcgggatc gtcaccgtaa tctgcttgca cgtagatcac ataagcacca 7261 agcgcgttgg cctcatgctt gaggagattg atgagcgcgg tggcaatgcc ctgcctccgg 7321 tgctcgccgg agactgcgag atcatagata tagatctcac tacgcggctg ctcaaacttg 7381 ggcagaacgt aagccgcgag agcgccaaca accgcttctt ggtcgaaggc agcaagcgcg 7441 atgaatgtct tactacggag caagttcccg aggtaatcgg agtccggctg atgttgggag 7501 taggtggcta cgtctccgaa ctcacgaccg aaaagatcaa gagcagcccg catggatttg 7561 acttggtcag ggccgagcct acatgtgcga atgatgccca tacttgagcc acctaacttt 7621 gttttagggc gactgccctg ctgcgtaaca tcgttgctgc tgcgtaacat cgttgctgct 7681 ccataacatc aaacatcgac ccacggcgta acgcgcttgc tgcttggatg cccgaggcat 7741 agactgtaca aaaaaacagt cataacaagc catgaaaacc gccactgcgc cgttaccacc 7801 gctgcgttcg gtcaaggttc tggaccagtt gcgtgagcgc atacgctact tgcattacag 7861 tttacgaacc gaacaggctt atgtcaactg ggttcgtgcc ttcatccgtt tccacggtgt 7921 gcgtcacccg gcaaccttgg gcagcagcga agtcgaggca tttctgtcct ggctggcgaa 7981 cgagcgcaag gtttcggtct ccacgcatcg tcaggcattg gcggccttgc tgttcttcta 8041 cggcaaggtg ctgtgcacgg atctgccctg gcttcaggag atcggaagac ctcggccgtc 8101 gcggcgcttg ccggtggtgc tgaccccgga tgaagtggtt cgcatcctcg gttttctgga 8161 aggcgagcat cgtttgttcg cccaggactc tagctatagt tctagtggtt ggctactaat 8221 aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct 8281 atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg 8341 tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta 8401 atcagaattg gttaattggt tgtaacactg gcagagcatt acgctgactt gacgggacgg 8461 cgcaagctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc 8521 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg 8581 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact 8641 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg 8701 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg 8761 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac 8821 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca 8881 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga 8941 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc 9001 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct 9061 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg 9121 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct 9181 tttgctcaca tgttctttcc tgcgttatcc c //