LOCUS Exported 3666 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Gateway-compatible Entry vector, with insert of NSP6 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. ACCESSION . VERSION . KEYWORDS pDONR223 SARS-CoV-2 NSP6 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3666) AUTHORS Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale M, Raught B, Jacob Y, Roth FP TITLE A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions. JOURNAL G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 10.1534/g3.120.401554. PUBMED 32763951 REFERENCE 2 (bases 1 to 3666) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3666 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 111..128 /label=L4440 /note="L4440 vector, forward primer" terminator 287..314 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 406..492 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 541..563 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 555..572 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 556..572 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 588..687 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(1564..1663) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1680..1699) /label=T7 /note="T7 promoter, forward primer" promoter complement(1681..1699) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1704..1720) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1704..1720) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" CDS 2151..2942 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 3035..3623 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3524..3543 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta 61 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag 121 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga 181 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg 241 caattaatac gcgtaccgct agccaggaag agtttgtaga aacgcaaaaa ggccatccgt 301 caggatggcc ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca 361 ccctccgggc cgttgcttca caacgttcaa atccgctccc ggcggatttg tcctactcag 421 gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtcttccg actgagcctt 481 tcgttttatt tgatgcctgg cagttcccta ctctcgcgtt aacgctagca tggatgtttt 541 cccagtcacg acgttgtaaa acgacggcca gtcttaagct cgggccccaa ataatgattt 601 tattttgact gatagtgacc tgttcgttgc aacaaattga tgagcaatgc ttttttataa 661 tgccaacttt gtacaaaaaa gttggcatga gcgccgtgaa gagaaccatc aagggaaccc 721 accactggct gctgctgacc atcctgacct ccctgctggt gctggtgcag tctacccagt 781 ggagcctgtt cttcttcctg tacgagaacg ccttcctgcc cttcgctatg ggtatcatcg 841 ccatgtccgc cttcgctatg atgttcgtga agcacaagca cgctttcctg tgcctgttcc 901 tgctgccatc cctggccacc gtggcttact tcaacatggt gtacatgccc gcctcttggg 961 tcatgcgcat catgacctgg ctggacatgg tggacaccag cctgtccggc ttcaagctga 1021 aggactgcgt gatgtacgcc tctgctgtgg tgctgctgat cctgatgacc gctagaaccg 1081 tgtacgatga tggtgctaga agggtgtgga ccctgatgaa cgtgctgacc ctggtgtaca 1141 aggtgtacta cggcaacgcc ctggaccagg ctatcagcat gtgggctctg atcatctctg 1201 tgaccagcaa ctactccgga gtggtgacca ccgtgatgtt cctggccaga ggtatcgtgt 1261 tcatgtgcgt ggagtactgc cctatcttct tcatcaccgg caacaccctg cagtgcatca 1321 tgctggtgta ctgcttcctg ggttacttct gcacctgcta tttcggcctg ttctgcctgc 1381 tgaacagata cttccgtctg accctgggcg tgtacgacta cctggtgtcc acccaggaat 1441 tccgttacat gaactctcag ggactgctgc cccctaagaa cagcatcgac gctttcaagc 1501 tgaacatcaa gctgctggga gtgggcggaa agccatgcat caaggtggcc accgtgcagt 1561 aatacccaac tttcttgtac aaagttggca ttataagaaa gcattgctta tcaatttgtt 1621 gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttgccatcca gctgatatcc 1681 cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc 1741 aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgcctc ctctagacca 1801 gccaggacag aaatgcctcg acttcgctgc tacccaaggt tgccgggtga cgcacaccgt 1861 ggaaacggat gaaggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat 1921 gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta 1981 acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc 2041 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca 2101 gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatt atgagggaag 2161 cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc 2221 tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc 2281 cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc 2341 gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc 2401 gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta 2461 agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc 2521 cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg 2581 ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg 2641 aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc 2701 gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc 2761 cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca 2821 tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag 2881 atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat 2941 aaccctcgag ccacccatga ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag 3001 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa 3061 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag 3121 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg 3181 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat 3241 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta 3301 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg 3361 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc 3421 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa 3481 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc 3541 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt 3601 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct 3661 tttgct //