LOCUS Exported 3135 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Gateway-compatible Entry vector, with insert of NSP9 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. ACCESSION . VERSION . KEYWORDS pDONR223 SARS-CoV-2 NSP9 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3135) AUTHORS Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale M, Raught B, Jacob Y, Roth FP TITLE A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions. JOURNAL G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 10.1534/g3.120.401554. PUBMED 32763951 REFERENCE 2 (bases 1 to 3135) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3135 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 100..117 /label=L4440 /note="L4440 vector, forward primer" terminator 276..303 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 395..481 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 530..552 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 544..561 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 545..561 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 577..676 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(1022..1121) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1138..1157) /label=T7 /note="T7 promoter, forward primer" promoter complement(1139..1157) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1162..1178) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1162..1178) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" CDS 1609..2400 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 2493..3081 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2982..3001 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag 61 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa 121 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc 181 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatacg 241 cgtaccgcta gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct 301 tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc 361 gttgcttcac aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca 421 ccgacaaaca acagataaaa cgaaaggccc agtcttccga ctgagccttt cgttttattt 481 gatgcctggc agttccctac tctcgcgtta acgctagcat ggatgttttc ccagtcacga 541 cgttgtaaaa cgacggccag tcttaagctc gggccccaaa taatgatttt attttgactg 601 atagtgacct gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg 661 tacaaaaaag ttggcatgaa caacgagctg agccctgtgg ccctgagaca gatgtcttgt 721 gctgctggta ccacccagac cgcttgcacc gacgacaacg ccctggctta ctacaacacc 781 accaagggcg gaagattcgt gctggccctg ctgtctgacc tgcaggacct gaagtgggct 841 cgtttcccca agagcgacgg taccggcacc atctacaccg agctggaacc cccttgcaga 901 ttcgtgaccg acaccccaaa gggccccaag gtgaagtacc tgtacttcat caagggactg 961 aacaacctga acagaggaat ggtgctgggt agcctggctg ctaccgtgag gctgcagtaa 1021 tacccaactt tcttgtacaa agttggcatt ataagaaagc attgcttatc aatttgttgc 1081 aacgaacagg tcactatcag tcaaaataaa atcattattt gccatccagc tgatatcccc 1141 tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc cgtgtctcaa 1201 aatctctgat gttacattgc acaagataaa aatatatcat catgcctcct ctagaccagc 1261 caggacagaa atgcctcgac ttcgctgcta cccaaggttg ccgggtgacg cacaccgtgg 1321 aaacggatga aggcacgaac ccagtggaca taagcctgtt cggttcgtaa gctgtaatgc 1381 aagtagcgta tgcgctcacg caactggtcc agaaccttga ccgaacgcag cggtggtaac 1441 ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttggggtaca gtctatgcct 1501 cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc 1561 aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taaacattat gagggaagcg 1621 gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc 1681 gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca 1741 cacagtgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga 1801 gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc 1861 gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag 1921 cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca 1981 gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc 2041 ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag 2101 gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga 2161 aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg 2221 aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata 2281 cttgaagcta gacaggctta tcttggacaa gaagaagatc gcttggcctc gcgcgcagat 2341 cagttggaag aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa 2401 ccctcgagcc acccatgacc aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg 2461 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc 2521 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag 2581 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt 2641 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac 2701 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc 2761 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt 2821 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt 2881 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc 2941 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt 3001 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca 3061 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt 3121 tgctggcctt ttgct //