LOCUS Exported 3213 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Gateway-compatible Entry vector, with insert of NSP10 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. ACCESSION . VERSION . KEYWORDS pDONR223 SARS-CoV-2 NSP10 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3213) AUTHORS Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale M, Raught B, Jacob Y, Roth FP TITLE A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions. JOURNAL G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 10.1534/g3.120.401554. PUBMED 32763951 REFERENCE 2 (bases 1 to 3213) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3213 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 75..91 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 75..91 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 96..115 /label=T7 /note="T7 promoter, forward primer" promoter 96..114 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind 132..231 /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(655..754) /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(770..787) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(770..786) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(779..801) /label=M13/pUC Forward /note="In lacZ gene" terminator 850..936 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" terminator 1028..1055 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" primer_bind complement(1214..1231) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(1385..1973) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1465..1484) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(2066..2857) /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" ORIGIN 1 agaggaggca tgatgatata tttttatctt gtgcaatgta acatcagaga ttttgagaca 61 cgggccagag ctgccaggaa acagctatga ccatgtaata cgactcacta taggggatat 121 cagctggatg gcaaataatg attttatttt gactgatagt gacctgttcg ttgcaacaaa 181 ttgataagca atgctttctt ataatgccaa ctttgtacaa gaaagttggg tattactgca 241 gcataggttc cctcagctgg tcgcaagagc atccgtagcc cttccacatt ccgcacacgg 301 tgcacacggt gttcttcagg gtgaatccca cggggtcgtt agcgcaggtg gttgggatct 361 gcacgtactt gcccttcagg tcgcagaaac ccttagggtt ggggtggtcg atgtggcatc 421 tgcagtacag gcagcagctg gcgccaccga aagattcctg gtccatgtta gcctctgggg 481 tcacggtgat ggcctgaccg gttccggtgt gggtgcacag catcttcacg cagttggtga 541 taggctgtcc gccggaagcc aggtagtcct tgtaggcctt agcggcgtcc acagcgaagg 601 cgcagaagga cagcacggtg ctgttagcag gcacctcagt agcgttacca gccatgccaa 661 cttttttgta caaagttggc attataaaaa agcattgctc atcaatttgt tgcaacgaac 721 aggtcactat cagtcaaaat aaaatcatta tttggggccc gagcttaaga ctggccgtcg 781 ttttacaacg tcgtgactgg gaaaacatcc atgctagcgt taacgcgaga gtagggaact 841 gccaggcatc aaataaaacg aaaggctcag tcggaagact gggcctttcg ttttatctgt 901 tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgggagcgga tttgaacgtt 961 gtgaagcaac ggcccggagg gtggcgggca ggacgcccgc cataaactgc caggcatcaa 1021 actaagcaga aggccatcct gacggatggc ctttttgcgt ttctacaaac tcttcctggc 1081 tagcggtacg cgtattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt 1141 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc 1201 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 1261 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 1321 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 1381 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 1441 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 1501 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 1561 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 1621 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 1681 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 1741 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 1801 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag 1861 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 1921 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 1981 ctttgatctt ttctacgggg tctgacgctc agtggaacga cgcgtaactc acgttaaggg 2041 attttggtca tgggtggctc gagggttatt tgccgactac cttggtgatc tcgcctttca 2101 cgtagtggac aaattcttcc aactgatctg cgcgcgaggc caagcgatct tcttcttgtc 2161 caagataagc ctgtctagct tcaagtatga cgggctgata ctgggccggc aggcgctcca 2221 ttgcccagtc ggcagcgaca tccttcggcg cgattttgcc ggttactgcg ctgtaccaaa 2281 tgcgggacaa cgtaagcact acatttcgct catcgccagc ccagtcgggc ggcgagttcc 2341 atagcgttaa ggtttcattt agcgcctcaa atagatcctg ttcaggaacc ggatcaaaga 2401 gttcctccgc cgctggacct accaaggcaa cgctatgttc tcttgctttt gtcagcaaga 2461 tagccagatc aatgtcgatc gtggctggct cgaagatacc tgcaagaatg tcattgcgct 2521 gccattctcc aaattgcagt tcgcgcttag ctggataacg ccacggaatg atgtcgtcgt 2581 gcacaacaat ggtgacttct acagcgcgga gaatctcgct ctctccaggg gaagccgaag 2641 tttccaaaag gtcgttgatc aaagctcgcc gcgttgtttc atcaagcctt acggtcaccg 2701 taaccagcaa atcaatatca ctgtgtggct tcaggccgcc atccactgcg gagccgtaca 2761 aatgtacggc cagcaacgtc ggttcgagat ggcgctcgat gacgccaact acctctgata 2821 gttgagtcga tacttcggcg atcaccgctt ccctcataat gtttaacttt gttttagggc 2881 gactgccctg ctgcgtaaca tcgttgctgc tccataacat caaacatcga cccacggcgt 2941 aacgcgcttg ctgcttggat gcccgaggca tagactgtac cccaaaaaaa cagtcataac 3001 aagccatgaa aaccgccact gcgccgttac caccgctgcg ttcggtcaag gttctggacc 3061 agttgcgtga gcgcatacgc tacttgcatt acagcttacg aaccgaacag gcttatgtcc 3121 actgggttcg tgccttcatc cgtttccacg gtgtgcgtca cccggcaacc ttgggtagca 3181 gcgaagtcga ggcatttctg tcctggctgg tct //