LOCUS Exported 784 bp ds-DNA linear SYN 26-MAY-2021 DEFINITION Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1) high affinity 2. ACCESSION . VERSION . KEYWORDS pcDNA3-sACE2v2-Fc(IgG1) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 784) AUTHORS Chan KK, Dorosky D, Sharma P, Abbasi SA, Dye JM, Kranz DM, Herbert AS, Procko E TITLE Engineering human ACE2 to optimize binding to the spike protein of SARS coronavirus 2. JOURNAL Science. 2020 Sep 4;369(6508):1261-1265. doi: 10.1126/science.abc0870. Epub 2020 Aug 4. PUBMED 32753553 REFERENCE 2 (bases 1 to 784) AUTHORS . TITLE Direct Submission JOURNAL Exported May 26, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..784 /organism="synthetic DNA construct" /mol_type="other DNA" polyA_signal 261..382 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(298..317) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 352..371 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(431..447) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(431..447) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(444..466) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 455..471 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(479..509) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 524..545 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(662..679) /label=L4440 /note="L4440 vector, forward primer" ORIGIN 1 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc 61 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag 121 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg 181 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc 241 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca 301 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt 361 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg 421 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca 481 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca 541 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc 601 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt 661 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact 721 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag 781 caaa //