LOCUS Exported 9266 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Endogenous tagging of GluN1: N-terminal (amino acid position: D23). ACCESSION . VERSION . KEYWORDS pORANGE GFP-Grin1 KI #2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9266) AUTHORS Willems J, de Jong APH, Scheefhals N, Mertens E, Catsburg LAE, Poorthuis RB, de Winter F, Verhaagen J, Meye FJ, MacGillavry HD TITLE ORANGE: A CRISPR/Cas9-based genome editing toolbox for epitope tagging of endogenous proteins in neurons. JOURNAL PLoS Biol. 2020 Apr 10;18(4):e3000665. doi: 10.1371/journal.pbio.3000665. PUBMED 32275651 REFERENCE 2 (bases 1 to 9266) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9266 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 62..302 /label=U6 promoter /note="RNA polymerase III promoter for human U6 snRNA" primer_bind 62..82 /label=hU6-F /note="Human U6 promoter, forward primer" primer_bind 233..252 /label=LKO.1 5' /note="Human U6 promoter, forward primer" misc_RNA 331..406 /label=gRNA scaffold /note="guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" CDS 536..1246 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE FVTAAGITLGMDELY" CDS 536..1246 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE FVTAAGITLGMDELY" primer_bind complement(578..599) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(839..858) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 1186..1207 /label=EGFP-C /note="EGFP, forward primer" enhancer 1331..1616 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer; contains an 18-bp deletion relative to the standard CMV enhancer" promoter 1618..1895 /label=chicken beta-actin promoter intron 1896..2123 /label=hybrid intron /note="hybrid between chicken beta-actin (CBA) and minute virus of mice (MMV) introns (Gray et al., 2011)" CDS 2144..2170 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" CDS 2174..2194 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 2204..6304 /codon_start=1 /product="Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=Cas9 /note="generates RNA-guided double strand breaks in DNA" /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD ATLIHQSITGLYETRIDLSQLGGD" CDS 6308..6328 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" primer_bind complement(6368..6385) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 6374..6581 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" repeat_region 6590..6730 /label=AAV2 ITR /note="inverted terminal repeat of adeno-associated virus serotype 2" repeat_region 6590..6719 /label=AAV2 ITR rep_origin 6805..7260 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(6892..6911) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 7102..7123 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(7277..7296) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 7396..7418 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(7456..7474) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 7542..7646 /gene="bla" /label=AmpR promoter CDS 7647..8507 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(7865..7884) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 8678..9266 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 9167..9186 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg 61 tgagggccta tttcccatga ttccttcata tttgcatata cgatacaagg ctgttagaga 121 gataattgga attaatttga ctgtaaacac aaagatatta gtacaaaata cgtgacgtag 181 aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa tggactatca 241 tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg 301 acgaaacacc gttgacgatc ttggggtcgc gttttagagc tagaaatagc aagttaaaat 361 aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttgttttaga 421 gctagaaata gcaagttaaa ataaggctag tccgttttta gcgcgtgcgc caattctgca 481 gacaaatggc tctagaagct tgttgacgat cttggggtcg caggcagcta gcggggtgag 541 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt 601 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct 661 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac 721 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga 781 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga 841 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg 901 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga 961 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa 1021 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta 1081 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag 1141 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga 1201 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtactcta ggggaagcgc 1261 ttcgttgacg atcttggggt cgcaggctcg aggatcctag gacgcgtcct aggacgcgtc 1321 cagaggtacc cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 1381 cccgcccatt gacgtcaata gtaacgccaa tagggacttt ccattgacgt caatgggtgg 1441 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc 1501 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttgtgcccag tacatgacct 1561 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtcg 1621 aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt 1681 tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg gggggggggc 1741 gcgcgccagg cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg agaggtgcgg 1801 cggcagccaa tcagagcggc gcgctccgaa agtttccttt tatggcgagg cggcggcggc 1861 ggcggcccta taaaaagcga agcgcgcggc gggcgggagt cgctgcgcgc tgccttcgcc 1921 ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg accgcgttac 1981 tcccacaggt gagcgggcgg gacggccctt ctcctccggg ctgtaattag ctgagcaaga 2041 ggtaagggtt taagggatgg ttggttggtg gggtattaat gtttaattac ctggagcacc 2101 tgcctgaaat cacttttttt caggttggac cggtgccacc atgtacccat acgatgttcc 2161 agattacgct tcgccgaaga aaaagcgcaa ggtcgaagcg tccgacaaga agtacagcat 2221 cggcctggac atcggcacca actctgtggg ctgggccgtg atcaccgacg agtacaaggt 2281 gcccagcaag aaattcaagg tgctgggcaa caccgaccgg cacagcatca agaagaacct 2341 gatcggagcc ctgctgttcg acagcggcga aacagccgag gccacccggc tgaagagaac 2401 cgccagaaga agatacacca gacggaagaa ccggatctgc tatctgcaag agatcttcag 2461 caacgagatg gccaaggtgg acgacagctt cttccacaga ctggaagagt ccttcctggt 2521 ggaagaggat aagaagcacg agcggcaccc catcttcggc aacatcgtgg acgaggtggc 2581 ctaccacgag aagtacccca ccatctacca cctgagaaag aaactggtgg acagcaccga 2641 caaggccgac ctgcggctga tctatctggc cctggcccac atgatcaagt tccggggcca 2701 cttcctgatc gagggcgacc tgaaccccga caacagcgac gtggacaagc tgttcatcca 2761 gctggtgcag acctacaacc agctgttcga ggaaaacccc atcaacgcca gcggcgtgga 2821 cgccaaggcc atcctgtctg ccagactgag caagagcaga cggctggaaa atctgatcgc 2881 ccagctgccc ggcgagaaga agaatggcct gttcggaaac ctgattgccc tgagcctggg 2941 cctgaccccc aacttcaaga gcaacttcga cctggccgag gatgccaaac tgcagctgag 3001 caaggacacc tacgacgacg acctggacaa cctgctggcc cagatcggcg accagtacgc 3061 cgacctgttt ctggccgcca agaacctgtc cgacgccatc ctgctgagcg acatcctgag 3121 agtgaacacc gagatcacca aggcccccct gagcgcctct atgatcaaga gatacgacga 3181 gcaccaccag gacctgaccc tgctgaaagc tctcgtgcgg cagcagctgc ctgagaagta 3241 caaagagatt ttcttcgacc agagcaagaa cggctacgcc ggctacattg acggcggagc 3301 cagccaggaa gagttctaca agttcatcaa gcccatcctg gaaaagatgg acggcaccga 3361 ggaactgctc gtgaagctga acagagagga cctgctgcgg aagcagcgga ccttcgacaa 3421 cggcagcatc ccccaccaga tccacctggg agagctgcac gccattctgc ggcggcagga 3481 agatttttac ccattcctga aggacaaccg ggaaaagatc gagaagatcc tgaccttccg 3541 catcccctac tacgtgggcc ctctggccag gggaaacagc agattcgcct ggatgaccag 3601 aaagagcgag gaaaccatca ccccctggaa cttcgaggaa gtggtggaca agggcgcttc 3661 cgcccagagc ttcatcgagc ggatgaccaa cttcgataag aacctgccca acgagaaggt 3721 gctgcccaag cacagcctgc tgtacgagta cttcaccgtg tataacgagc tgaccaaagt 3781 gaaatacgtg accgagggaa tgagaaagcc cgccttcctg agcggcgagc agaaaaaggc 3841 catcgtggac ctgctgttca agaccaaccg gaaagtgacc gtgaagcagc tgaaagagga 3901 ctacttcaag aaaatcgagt gcttcgactc cgtggaaatc tccggcgtgg aagatcggtt 3961 caacgcctcc ctgggcacat accacgatct gctgaaaatt atcaaggaca aggacttcct 4021 ggacaatgag gaaaacgagg acattctgga agatatcgtg ctgaccctga cactgtttga 4081 ggacagagag atgatcgagg aacggctgaa aacctatgcc cacctgttcg acgacaaagt 4141 gatgaagcag ctgaagcggc ggagatacac cggctggggc aggctgagcc ggaagctgat 4201 caacggcatc cgggacaagc agtccggcaa gacaatcctg gatttcctga agtccgacgg 4261 cttcgccaac agaaacttca tgcagctgat ccacgacgac agcctgacct ttaaagagga 4321 catccagaaa gcccaggtgt ccggccaggg cgatagcctg cacgagcaca ttgccaatct 4381 ggccggcagc cccgccatta agaagggcat cctgcagaca gtgaaggtgg tggacgagct 4441 cgtgaaagtg atgggccggc acaagcccga gaacatcgtg atcgaaatgg ccagagagaa 4501 ccagaccacc cagaagggac agaagaacag ccgcgagaga atgaagcgga tcgaagaggg 4561 catcaaagag ctgggcagcc agatcctgaa agaacacccc gtggaaaaca cccagctgca 4621 gaacgagaag ctgtacctgt actacctgca gaatgggcgg gatatgtacg tggaccagga 4681 actggacatc aaccggctgt ccgactacga tgtggaccat atcgtgcctc agagctttct 4741 gaaggacgac tccatcgaca acaaggtgct gaccagaagc gacaagaacc ggggcaagag 4801 cgacaacgtg ccctccgaag aggtcgtgaa gaagatgaag aactactggc ggcagctgct 4861 gaacgccaag ctgattaccc agagaaagtt cgacaatctg accaaggccg agagaggcgg 4921 cctgagcgaa ctggataagg ccggcttcat caagagacag ctggtggaaa cccggcagat 4981 cacaaagcac gtggcacaga tcctggactc ccggatgaac actaagtacg acgagaatga 5041 caagctgatc cgggaagtga aagtgatcac cctgaagtcc aagctggtgt ccgatttccg 5101 gaaggatttc cagttttaca aagtgcgcga gatcaacaac taccaccacg cccacgacgc 5161 ctacctgaac gccgtcgtgg gaaccgccct gatcaaaaag taccctaagc tggaaagcga 5221 gttcgtgtac ggcgactaca aggtgtacga cgtgcggaag atgatcgcca agagcgagca 5281 ggaaatcggc aaggctaccg ccaagtactt cttctacagc aacatcatga actttttcaa 5341 gaccgagatt accctggcca acggcgagat ccggaagcgg cctctgatcg agacaaacgg 5401 cgaaaccggg gagatcgtgt gggataaggg ccgggatttt gccaccgtgc ggaaagtgct 5461 gagcatgccc caagtgaata tcgtgaaaaa gaccgaggtg cagacaggcg gcttcagcaa 5521 agagtctatc ctgcccaaga ggaacagcga taagctgatc gccagaaaga aggactggga 5581 ccctaagaag tacggcggct tcgacagccc caccgtggcc tattctgtgc tggtggtggc 5641 caaagtggaa aagggcaagt ccaagaaact gaagagtgtg aaagagctgc tggggatcac 5701 catcatggaa agaagcagct tcgagaagaa tcccatcgac tttctggaag ccaagggcta 5761 caaagaagtg aaaaaggacc tgatcatcaa gctgcctaag tactccctgt tcgagctgga 5821 aaacggccgg aagagaatgc tggcctctgc cggcgaactg cagaagggaa acgaactggc 5881 cctgccctcc aaatatgtga acttcctgta cctggccagc cactatgaga agctgaaggg 5941 ctcccccgag gataatgagc agaaacagct gtttgtggaa cagcacaagc actacctgga 6001 cgagatcatc gagcagatca gcgagttctc caagagagtg atcctggccg acgctaatct 6061 ggacaaagtg ctgtccgcct acaacaagca ccgggataag cccatcagag agcaggccga 6121 gaatatcatc cacctgttta ccctgaccaa tctgggagcc cctgccgcct tcaagtactt 6181 tgacaccacc atcgaccgga agaggtacac cagcaccaaa gaggtgctgg acgccaccct 6241 gatccaccag agcatcaccg gcctgtacga gacacggatc gacctgtctc agctgggagg 6301 cgacagcccc aagaagaaga gaaaggtgga ggccagctaa gaattctaac tagagctcgc 6361 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg 6421 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt 6481 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc 6541 aagggggagg attgggaaga gaatagcagg catgctgggg agcggccgca ggaaccccta 6601 gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca 6661 aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc 6721 tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac 6781 cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg 6841 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cttagcgccc gctcctttcg 6901 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg 6961 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt 7021 tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt 7081 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaactcta 7141 tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggtctat tggttaaaaa 7201 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt 7261 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc 7321 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac 7381 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac 7441 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa 7501 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt 7561 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc 7621 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc 7681 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa 7741 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg 7801 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag 7861 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc 7921 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta 7981 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg 8041 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca 8101 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac 8161 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat 8221 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg 8281 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata 8341 aatctggagc cggtgagcgt ggaagccgcg gtatcattgc agcactgggg ccagatggta 8401 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa 8461 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag 8521 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg 8581 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact 8641 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg 8701 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc 8761 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata 8821 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta 8881 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc 8941 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg 9001 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac 9061 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg 9121 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt 9181 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct 9241 cgtcaggggg gcggagccta tggaaa //