LOCUS Exported 9830 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION doxycycline-inducible expression of Human RUNX1 in mammalian cells. ACCESSION . VERSION . KEYWORDS pFUW-tetO-RUNX1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9830) AUTHORS Rosa FF, Pires CF, Kurochkin I, Ferreira AG, Gomes AM, Palma LG, Shaiv K, Solanas L, Azenha C, Papatsenko D, Schulz O, Reis e Sousa C, Pereira CF TITLE Direct reprogramming of fibroblasts into antigen-presenting dendritic cells. JOURNAL Sci Immunol. 2018 Dec 7;3(30). pii: 3/30/eaau4292. doi: 10.1126/sciimmunol.aau4292. PUBMED 30530727 REFERENCE 2 (bases 1 to 9830) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9830 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 13..283 /label=tetracycline response element /bound_moiety="tetracycline repressor TetR" /note="contains seven copies of the tetracycline operator tetO" promoter 316..354 /label=minimal CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 351..375 /label=LNCX /note="Human CMV promoter, forward primer" misc_feature 1921..2509 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(1974..1994) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(2392..2403) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(2512..2528) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2513..2529) /label=pBluescriptKS /note="For pBluescript vector" LTR 3034..3214 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" primer_bind complement(3240..3257) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 3246..3470 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 3516..3944 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(3603..3622) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 3813..3834 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(3953..3973) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 3958..4287 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 4138..4273 /label=SV40 ori /note="SV40 origin of replication" primer_bind 4200..4219 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 4335..4382 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 4401..4775 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" polyA_signal 4905..5026 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(4942..4961) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 4996..5015 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(5075..5091) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(5075..5091) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(5088..5110) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 5099..5115 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(5123..5153) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 5168..5189 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(5306..5323) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(5477..6065) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(5557..5576) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(6236..7096) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 6859..6878 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(7097..7201) /gene="bla" /label=AmpR promoter primer_bind complement(7276..7295) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 7467..7846 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 7848..8046 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 8001..8021 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" LTR 8064..8244 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 8291..8416 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 8909..9142 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 9327..9371 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 9669..9786 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" ORIGIN 1 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta 61 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt 121 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac 181 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag 241 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagct 301 cggtacccgg gtcgaggtag gcgtgtacgg tgggaggcct atataagcag agctcgttta 361 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac 421 cgggaccgat ccagcctccg cggccccgaa ttcatggctt cagacagcat atttgagtca 481 tttccttcgt acccacagtg cttcatgaga gaatgcatac ttggaatgaa tccttctaga 541 gacgtccacg atgccagcac gagccgccgc ttcacgccgc cttccaccgc gctgagccca 601 ggcaagatga gcgaggcgtt gccgctgggc gccccggacg ccggcgctgc cctggccggc 661 aagctgagga gcggcgaccg cagcatggtg gaggtgctgg ccgaccaccc gggcgagctg 721 gtgcgcaccg acagccccaa cttcctctgc tccgtgctgc ctacgcactg gcgctgcaac 781 aagaccctgc ccatcgcttt caaggtggtg gccctagggg atgttccaga tggcactctg 841 gtcactgtga tggctggcaa tgatgaaaac tactcggctg agctgagaaa tgctaccgca 901 gccatgaaga accaggttgc aagatttaat gacctcaggt ttgtcggtcg aagtggaaga 961 gggaaaagct tcactctgac catcactgtc ttcacaaacc caccgcaagt cgccacctac 1021 cacagagcca tcaaaatcac agtggatggg ccccgagaac ctcgaagaca tcggcagaaa 1081 ctagatgatc agaccaagcc cgggagcttg tccttttccg agcggctcag tgaactggag 1141 cagctgcggc gcacagccat gagggtcagc ccacaccacc cagcccccac gcccaaccct 1201 cgtgcctccc tgaaccactc cactgccttt aaccctcagc ctcagagtca gatgcaggat 1261 acaaggcaga tccaaccatc cccaccgtgg tcctacgatc agtcctacca atacctggga 1321 tccattgcct ctccttctgt gcacccagca acgcccattt cacctggacg tgccagcggc 1381 atgacaaccc tctctgcaga actttccagt cgactctcaa cggcacccga cctgacagcg 1441 ttcagcgacc cgcgccagtt ccccgcgctg ccctccatct ccgacccccg catgcactat 1501 ccaggcgcct tcacctactc cccgacgccg gtcacctcgg gcatcggcat cggcatgtcg 1561 gccatgggct cggccacgcg ctaccacacc tacctgccgc cgccctaccc cggctcgtcg 1621 caagcgcagg gaggcccgtt ccaagccagc tcgccctcct accacctgta ctacggcgcc 1681 tcggccggct cctaccagtt ctccatggtg ggcggcgagc gctcgccgcc gcgcatcctg 1741 ccgccctgca ccaacgcctc caccggctcc gcgctgctca accccagcct cccgaaccag 1801 agcgacgtgg tggaggccga gggcagccac agcaactccc ccaccaacat ggcgccctcc 1861 gcgcgcctgg aggaggccgt gtggcggccc tactgagaat tcgatatcaa gcttatcgat 1921 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct 1981 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt 2041 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg 2101 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact 2161 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct 2221 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg 2281 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc 2341 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc 2401 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt 2461 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt 2521 cgacctcgag acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat 2581 gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct 2641 caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa 2701 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg 2761 tggatctacc acacacaagg ctacttccct gattggcaga actacacacc agggccaggg 2821 atcagatatc cactgacctt tggatggtgc tacaagctag taccagttga gcaagagaag 2881 gtagaagaag ccaatgaagg agagaacacc cgcttgttac accctgtgag cctgcatggg 2941 atggatgacc cggagagaga agtattagag tggaggtttg acagccgcct agcatttcat 3001 cacatggccc gagagctgca tccggactgt actgggtctc tctggttaga ccagatctga 3061 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 3121 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 3181 agaccctttt agtcagtgtg gaaaatctct agcagggccc gtttaaaccc gctgatcagc 3241 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt 3301 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca 3361 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga 3421 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc 3481 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag 3541 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc 3601 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc 3661 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa 3721 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg 3781 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac 3841 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta 3901 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg 3961 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg 4021 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt 4081 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc 4141 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt 4201 atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc 4261 ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga 4321 tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg 4381 acaaggtgag gaactaaacc atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc 4441 gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc cgggacttcg 4501 tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc agcgcggtcc 4561 aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc 4621 tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca 4681 tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac ccggccggca 4741 actgcgtgca cttcgtggcc gaggagcagg actgacacgt gctacgagat ttcgattcca 4801 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga 4861 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag 4921 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt 4981 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac 5041 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt 5101 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg 5161 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt 5221 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt 5281 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 5341 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 5401 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 5461 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 5521 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 5581 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 5641 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 5701 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 5761 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 5821 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 5881 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc 5941 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 6001 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 6061 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 6121 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 6181 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 6241 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 6301 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 6361 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 6421 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 6481 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 6541 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 6601 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 6661 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 6721 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 6781 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 6841 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 6901 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 6961 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 7021 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 7081 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt 7141 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc 7201 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct 7261 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc 7321 tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca 7381 aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg 7441 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc 7501 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt 7561 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta 7621 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg 7681 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga 7741 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt 7801 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg 7861 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc 7921 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg 7981 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat 8041 aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc 8101 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc 8161 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt 8221 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa 8281 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga 8341 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga 8401 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg 8461 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga 8521 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat 8581 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa 8641 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc 8701 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc 8761 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat 8821 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg 8881 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag 8941 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt 9001 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt 9061 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat 9121 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca 9181 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca 9241 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa 9301 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat 9361 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca 9421 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga 9481 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg 9541 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca 9601 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc 9661 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta 9721 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa 9781 aattttcggg tttattacag ggacagcaga gatccagttt ggttagatct //