LOCUS       Exported                2757 bp ds-DNA     linear   SYN 25-JUN-2021
DEFINITION  Gateway-compatible Entry vector, with insert of NSP12 gene's CDS 
            from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon. .
ACCESSION   .
VERSION     .
KEYWORDS    pDONR223 SARS-CoV-2 NSP12_nostop
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 25, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
ORIGIN
        1 atgtccgccg ctagactgac tccttgtggt actggcacct ctactgacgt ggtgtacagg
       61 gccttcgaca tctacaacga caaggtggcc ggattcgcta agttcctgaa gaccaactgc
      121 tgccgcttcc aggagaagga cgaagacgac aacctgatcg actcttactt cgtggtgaag
      181 aggcacacct tcagcaacta ccagcacgag gaaaccatct acaacctgct gaaggactgc
      241 cccgccgtgg ctaagcacga cttcttcaag ttccgcatcg acggtgacat ggtgcctcac
      301 atcagccgcc agaggctgac caagtacacc atggccgacc tggtgtacgc tctgaggcac
      361 ttcgacgagg gcaactgcga caccctgaag gaaatcctgg tgacctacaa ctgctgcgac
      421 gacgactact tcaacaagaa ggactggtac gacttcgtgg agaaccctga catcctgaga
      481 gtgtacgcca acctgggcga aagagtgcgt caggctctgc tgaagaccgt gcagttctgc
      541 gacgccatgc gtaacgctgg tatcgtgggc gtgctgaccc tggacaacca ggacctgaac
      601 ggcaactggt acgacttcgg agacttcatc cagactactc caggatctgg tgtgcctgtg
      661 gtggactcct actactctct gctgatgcca atcctgaccc tgaccagggc cctgaccgct
      721 gagtctcacg tggacaccga cctgaccaag ccctacatca agtgggacct gctgaagtac
      781 gacttcaccg aggaacgcct gaagctgttc gacaggtact tcaagtactg ggaccagacc
      841 taccacccaa actgcgtgaa ctgcctggac gacagatgca tcctgcactg cgccaacttc
      901 aacgtgctgt tctctaccgt gttccctccc accagcttcg gccctctggt gcgtaagatc
      961 ttcgtggacg gcgtgccatt cgtggtgtct accggatacc acttcagaga gctgggtgtg
     1021 gtgcacaacc aggacgtgaa cctgcacagc tcccgtctga gcttcaagga actgctggtg
     1081 tacgctgctg accctgctat gcacgctgct agcggcaacc tgctgctgga caagcgcacc
     1141 acctgcttct ccgtggccgc tctgaccaac aacgtggcct tccagaccgt gaagcctgga
     1201 aacttcaaca aggacttcta cgacttcgct gtgagcaagg gattcttcaa ggagggttct
     1261 agcgtggaac tgaagcactt cttcttcgcc caggacggca acgccgctat ctccgactac
     1321 gactactacc gctacaacct gcccaccatg tgcgacatca ggcagctgct gttcgtggtg
     1381 gaggtggtgg acaagtactt cgactgctac gacggcggat gcatcaacgc taaccaggtc
     1441 atcgtgaaca acctggacaa gagcgccggc ttccctttca acaagtgggg aaaggctaga
     1501 ctgtactacg acagcatgtc ctacgaagac caggacgccc tgttcgctta caccaagcgt
     1561 aacgtgatcc caaccatcac ccagatgaac ctgaagtacg ccatctccgc taagaaccgc
     1621 gccaggaccg tggctggtgt gagcatctgc tccaccatga ccaacagaca gttccaccag
     1681 aagctgctga agtctatcgc cgctacccgt ggtgccaccg tggtcatcgg caccagcaag
     1741 ttctacggtg gctggcacaa catgctgaag accgtgtact ccgacgtgga gaacccacac
     1801 ctgatgggct gggactaccc caagtgcgac agagctatgc ctaacatgct gcgtatcatg
     1861 gcctctctgg tgctggctag aaagcacacc acctgctgct ctctgagcca ccgcttctac
     1921 aggctggcca acgagtgcgc tcaggtgctg tccgaaatgg tcatgtgcgg aggttctctg
     1981 tacgtgaagc ccggcggaac ctcctctgga gacgctacca ccgcctacgc taactccgtg
     2041 ttcaacatct gccaggccgt gaccgctaac gtgaacgccc tgctgtctac cgacggtaac
     2101 aagatcgctg acaagtacgt gagaaacctg cagcaccgtc tgtacgagtg cctgtacaga
     2161 aaccgtgacg tggacaccga cttcgtgaac gaattctacg cctacctgcg caagcacttc
     2221 tccatgatga tcctgtctga cgacgctgtg gtgtgcttca actctaccta cgccagccag
     2281 ggcctggtgg ctagcatcaa gaacttcaag tccgtgctgt actaccagaa caacgtgttc
     2341 atgagcgagg ccaagtgctg gaccgaaacc gacctgacca agggacctca cgagttctgc
     2401 tcccagcaca ccatgctggt gaagcagggt gacgactacg tgtacctgcc ataccctgac
     2461 ccttctagaa tcctgggagc tggatgcttc gtggacgaca tcgtgaagac cgacggcacc
     2521 ctgatgatcg aaaggttcgt gagcctggcc atcgacgctt accctctgac caagcaccca
     2581 aaccaggagt acgctgacgt gttccacctg tacctgcagt acatccgcaa gctgcacgac
     2641 gaactgaccg gacacatgct ggacatgtac agcgtgatgc tgaccaacga caacacctcc
     2701 aggtactggg agccagaatt ctacgaggcc atgtacaccc ctcacaccgt gctgcag
//
