LOCUS       Exported                9827 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  doxycycline-inducible expression of Human KLF4 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    pFUW-tetO-KLF4
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9827)
  AUTHORS   Rosa FF, Pires CF, Kurochkin I, Ferreira AG, Gomes AM, Palma LG, 
            Shaiv K, Solanas L, Azenha C, Papatsenko D, Schulz O, Reis e Sousa 
            C, Pereira CF
  TITLE     Direct reprogramming of fibroblasts into antigen-presenting 
            dendritic cells.
  JOURNAL   Sci Immunol. 2018 Dec 7;3(30). pii: 3/30/eaau4292. doi: 
            10.1126/sciimmunol.aau4292.
  PUBMED    30530727
REFERENCE   2  (bases 1 to 9827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9827
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    167..284
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    341..611
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        644..682
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     679..703
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    2246..2834
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2299..2319)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2717..2728)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2837..2853)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2838..2854)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3359..3539
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3565..3582)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3571..3795
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3841..4269
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3928..3947)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4138..4159
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4278..4298)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4283..4612
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4463..4598
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4525..4544
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4660..4707
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4726..5100
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5230..5351
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5267..5286)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5321..5340
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5400..5416)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5400..5416)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5413..5435)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5424..5440
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5448..5478)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5493..5514
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5631..5648)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5802..6390)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5882..5901)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6561..7421)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7184..7203
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7422..7526)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7601..7620)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7792..8171
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8173..8371
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8326..8346
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             8389..8569
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8616..8741
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9234..9467
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9652..9696
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat
       61 agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga acggatcggc
      121 actgcgtgcg ccaattctgc agacaaatgg cagtattcat ccacaatttt aaaagaaaag
      181 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
      241 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
      301 acagcagaga tccagtttgg ttagatctcg agtttaccac tccctatcag tgatagagaa
      361 aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta
      421 ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat
      481 agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
      541 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc
      601 agtgatagag aaaagtgaaa gtcgagctcg gtacccgggt cgaggtaggc gtgtacggtg
      661 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc
      721 acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgcg gccccgaatt
      781 catgaggcag ccacctggcg agtctgacat ggctgtcagc gacgcgctgc tcccatcttt
      841 ctccacgttc gcgtctggcc cggcgggaag ggagaagaca ctgcgtcaag caggtgcccc
      901 gaataaccgc tggcgggagg agctctccca catgaagcga cttcccccag tgcttcccgg
      961 ccgcccctat gacctggcgg cggcgaccgt ggccacagac ctggagagcg gcggagccgg
     1021 tgcggcttgc ggcggtagca acctggcgcc cctacctcgg agagagaccg aggagttcaa
     1081 cgatctcctg gacctggact ttattctctc caattcgctg acccatcctc cggagtcagt
     1141 ggccgccacc gtgtcctcgt cagcgtcagc ctcctcttcg tcgtcgccgt cgagcagcgg
     1201 ccctgccagc gcgccctcca cctgcagctt cacctatccg atccgggccg ggaacgaccc
     1261 gggcgtggcg ccgggcggca cgggcggagg cctcctctat ggcagggagt ccgctccccc
     1321 tccgacggct cccttcaacc tggcggacat caacgacgtg agcccctcgg gcggcttcgt
     1381 ggccgagctc ctgcggccag aattggaccc ggtgtacatt ccgccgcagc agccgcagcc
     1441 gccaggtggc gggctgatgg gcaagttcgt gctgaaggcg tcgctgagcg cccctggcag
     1501 cgagtacggc agcccgtcgg tcatcagcgt cagcaaaggc agccctgacg gcagccaccc
     1561 ggtggtggtg gcgccctaca acggcgggcc gccgcgcacg tgccccaaga tcaagcagga
     1621 ggcggtctct tcgtgcaccc acttgggcgc tggaccccct ctcagcaatg gccaccggcc
     1681 ggctgcacac gacttccccc tggggcggca gctccccagc aggactaccc cgaccctggg
     1741 tcttgaggaa gtgctgagca gcagggactg tcaccctgcc ctgccgcttc ctcccggctt
     1801 ccatccccac ccggggccca attacccatc cttcctgccc gatcagatgc agccgcaagt
     1861 cccgccgctc cattaccaag agctcatgcc acccggttcc tgcatgccag aggagcccaa
     1921 gccaaagagg ggaagacgat cgtggccccg gaaaaggacc gccacccaca cttgtgatta
     1981 cgcgggctgc ggcaaaacct acacaaagag ttcccatctc aaggcacacc tgcgaaccca
     2041 cacaggtgag aaaccttacc actgtgactg ggacggctgt ggatggaaat tcgcccgctc
     2101 agatgaactg accaggcact accgtaaaca cacggggcac cgcccgttcc agtgccaaaa
     2161 atgcgaccga gcattttcca ggtcggacca cctcgcctta cacatgaaga ggcattttta
     2221 agaattcgat atcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt
     2281 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     2341 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     2401 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     2461 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     2521 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     2581 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     2641 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     2701 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     2761 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     2821 ggccgcctcc ccgcatcgat accgtcgacc tcgagaccta gaaaaacatg gagcaatcac
     2881 aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga
     2941 ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc
     3001 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     3061 acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact tccctgattg
     3121 gcagaactac acaccagggc cagggatcag atatccactg acctttggat ggtgctacaa
     3181 gctagtacca gttgagcaag agaaggtaga agaagccaat gaaggagaga acacccgctt
     3241 gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtat tagagtggag
     3301 gtttgacagc cgcctagcat ttcatcacat ggcccgagag ctgcatccgg actgtactgg
     3361 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     3421 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     3481 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     3541 ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     3601 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     3661 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     3721 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     3781 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc
     3841 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     3901 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     3961 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     4021 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     4081 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     4141 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     4201 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     4261 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     4321 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     4381 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     4441 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     4501 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
     4561 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
     4621 gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca
     4681 tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc caagttgacc
     4741 agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac
     4801 cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac
     4861 gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg
     4921 gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac
     4981 ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag
     5041 ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga
     5101 cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     5161 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     5221 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     5281 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     5341 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
     5401 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     5461 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     5521 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     5581 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     5641 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     5701 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     5761 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     5821 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     5881 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     5941 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     6001 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     6061 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     6121 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     6181 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     6241 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     6301 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     6361 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     6421 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     6481 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     6541 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     6601 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     6661 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     6721 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     6781 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     6841 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     6901 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     6961 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     7021 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     7081 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     7141 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     7201 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     7261 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     7321 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     7381 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     7441 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     7501 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac
     7561 ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc tctgatgccg
     7621 catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga
     7681 gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta
     7741 gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat
     7801 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
     7861 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
     7921 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
     7981 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
     8041 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
     8101 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
     8161 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
     8221 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
     8281 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
     8341 ggcgtgtacg gtgggaggtc tatataagca gcgcgttttg cctgtactgg gtctctctgg
     8401 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     8461 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     8521 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
     8581 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
     8641 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     8701 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     8761 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     8821 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     8881 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     8941 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     9001 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     9061 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     9121 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     9181 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     9241 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     9301 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     9361 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     9421 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     9481 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     9541 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     9601 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     9661 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     9721 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     9781 gtttttgctg tactttctat agtgaataga gttaggcagg gatattc
//
