LOCUS Exported 10943 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION doxycycline-inducible expression of mouse Irf8 in mammalian cells. ACCESSION . VERSION . KEYWORDS pFUW-tetO-Irf8 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10943) AUTHORS Rosa FF, Pires CF, Kurochkin I, Ferreira AG, Gomes AM, Palma LG, Shaiv K, Solanas L, Azenha C, Papatsenko D, Schulz O, Reis e Sousa C, Pereira CF TITLE Direct reprogramming of fibroblasts into antigen-presenting dendritic cells. JOURNAL Sci Immunol. 2018 Dec 7;3(30). pii: 3/30/eaau4292. doi: 10.1126/sciimmunol.aau4292. PUBMED 30530727 REFERENCE 2 (bases 1 to 10943) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10943 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 167..284 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" protein_bind 341..611 /label=tetracycline response element /bound_moiety="tetracycline repressor TetR" /note="contains seven copies of the tetracycline operator tetO" promoter 644..682 /label=minimal CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 679..703 /label=LNCX /note="Human CMV promoter, forward primer" misc_feature 3362..3950 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(3415..3435) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(3833..3844) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(3953..3969) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(3954..3970) /label=pBluescriptKS /note="For pBluescript vector" LTR 4475..4655 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" primer_bind complement(4681..4698) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 4687..4911 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 4957..5385 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(5044..5063) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 5254..5275 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(5394..5414) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 5399..5728 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 5579..5714 /label=SV40 ori /note="SV40 origin of replication" primer_bind 5641..5660 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 5776..5823 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 5842..6216 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" polyA_signal 6346..6467 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(6383..6402) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 6437..6456 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(6516..6532) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(6516..6532) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(6529..6551) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 6540..6556 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(6564..6594) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 6609..6630 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(6747..6764) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(6918..7506) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(6998..7017) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(7677..8537) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 8300..8319 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(8538..8642) /gene="bla" /label=AmpR promoter primer_bind complement(8717..8736) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 8908..9287 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 9289..9487 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 9442..9462 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" LTR 9505..9685 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 9732..9857 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 10350..10583 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 10768..10812 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" ORIGIN 1 accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat 61 agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga acggatcggc 121 actgcgtgcg ccaattctgc agacaaatgg cagtattcat ccacaatttt aaaagaaaag 181 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 241 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 301 acagcagaga tccagtttgg ttagatctcg agtttaccac tccctatcag tgatagagaa 361 aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta 421 ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat 481 agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg 541 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc 601 agtgatagag aaaagtgaaa gtcgagctcg gtacccgggt cgaggtaggc gtgtacggtg 661 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc 721 acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgcg gccccgaatt 781 catgtgtgac cggaacggcg ggcggcggct gcggcagtgg ctgatcgaac agatcgacag 841 cagcatgtac ccggggctga tctgggaaaa tgatgagaag accatgttcc gtatcccctg 901 gaagcatgcc ggcaagcagg attacaatca ggaggtggat gcttccatct tcaaggcctg 961 ggcagttttt aaagggaagt ttaaagaggg agacaaagct gaaccagcca cgtggaagac 1021 gaggttacgc tgtgctctga acaagagccc agattttgaa gaagtgactg accggtccca 1081 gctggacatt tctgagccat ataaagttta ccgaattgtc cccgaggaag aacaaaaatg 1141 caagctgggc gtggcacctg caggctgcat gagcgaagtt cctgagatgg agtgtggccg 1201 ctcagagatt gaggagctga tcaaggaacc ttctgtggat gagtacatgg gtatgaccaa 1261 gaggagccca tccccaccag aggcctgcag gagccagatc ctccctgact ggtgggtcca 1321 gcagcccagt gcaggcctgc cactggtgac cggatatgcc gcctatgaca cacaccattc 1381 agctttctcc cagatggtca tcagcttcta ctacgggggc aagctggtgg gccaggccac 1441 caccacctgc cttgaaggct gccgtctctc cctgagccag ccggggctgc ctaagttgta 1501 tgggccggat ggcctggaac ccgtgtgctt tccgacggcc gacaccatcc ccagtgagcg 1561 gcagaggcag gtgacccgga agctgtttgg gcacctggaa cgtggcgtgc tactgcacag 1621 caaccgcaag ggcgtgttcg tgaagcggct gtgccagggc cgcgtgttct gcagcggcaa 1681 cgcggtggtg tgcaagggca ggcccaacaa gctggagcgg gacgaggtgg tgcaggtctt 1741 tgacaccaac cagttcatcc gagagctgca gcaattctac gccacccaga gccgcctacc 1801 tgacagcagg gtggtcctgt gcttcgggga ggagtttccg gacactgtgc ccttgcgctc 1861 caaactcatt ctggtgcagg tagagcagct gtatgccagg cagctggtgg aggaagcggg 1921 caagagctgc ggtgctggct ccctgatgcc agccctggag gagccccagc cggaccaggc 1981 tttccgcatg tttccggata tctgtacctc acaccagaga ccctttttta gagaaaatca 2041 acagatcacc gtctaagaat tcatgtgtga ccggaacggc gggcggcggc tgcggcagtg 2101 gctgatcgaa cagatcgaca gcagcatgta cccggggctg atctgggaaa atgatgagaa 2161 gaccatgttc cgtatcccct ggaagcatgc cggcaagcag gattacaatc aggaggtgga 2221 tgcttccatc ttcaaggcct gggcagtttt taaagggaag tttaaagagg gagacaaagc 2281 tgaaccagcc acgtggaaga cgaggttacg ctgtgctctg aacaagagcc cagattttga 2341 agaagtgact gaccggtccc agctggacat ttctgagcca tataaagttt accgaattgt 2401 ccccgaggaa gaacaaaaat gcaagctggg cgtggcacct gcaggctgca tgagcgaagt 2461 tcctgagatg gagtgtggcc gctcagagat tgaggagctg atcaaggaac cttctgtgga 2521 tgagtacatg ggtatgacca agaggagccc atccccacca gaggcctgca ggagccagat 2581 cctccctgac tggtgggtcc agcagcccag tgcaggcctg ccactggtga ccggatatgc 2641 cgcctatgac acacaccatt cagctttctc ccagatggtc atcagcttct actacggggg 2701 caagctggtg ggccaggcca ccaccacctg ccttgaaggc tgccgtctct ccctgagcca 2761 gccggggctg cctaagttgt atgggccgga tggcctggaa cccgtgtgct ttccgacggc 2821 cgacaccatc cccagtgagc ggcagaggca ggtgacccgg aagctgtttg ggcacctgga 2881 acgtggcgtg ctactgcaca gcaaccgcaa gggcgtgttc gtgaagcggc tgtgccaggg 2941 ccgcgtgttc tgcagcggca acgcggtggt gtgcaagggc aggcccaaca agctggagcg 3001 ggacgaggtg gtgcaggtct ttgacaccaa ccagttcatc cgagagctgc agcaattcta 3061 cgccacccag agccgcctac ctgacagcag ggtggtcctg tgcttcgggg aggagtttcc 3121 ggacactgtg cccttgcgct ccaaactcat tctggtgcag gtagagcagc tgtatgccag 3181 gcagctggtg gaggaagcgg gcaagagctg cggtgctggc tccctgatgc cagccctgga 3241 ggagccccag ccggaccagg ctttccgcat gtttccggat atctgtacct cacaccagag 3301 accctttttt agagaaaatc aacagatcac cgtctaagaa ttcgatatca agcttatcga 3361 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc 3421 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg 3481 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt 3541 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac 3601 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc 3661 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct 3721 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct 3781 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct 3841 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct 3901 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg 3961 tcgacctcga gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa 4021 tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc 4081 tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa 4141 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct 4201 gtggatctac cacacacaag gctacttccc tgattggcag aactacacac cagggccagg 4261 gatcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg agcaagagaa 4321 ggtagaagaa gccaatgaag gagagaacac ccgcttgtta caccctgtga gcctgcatgg 4381 gatggatgac ccggagagag aagtattaga gtggaggttt gacagccgcc tagcatttca 4441 tcacatggcc cgagagctgc atccggactg tactgggtct ctctggttag accagatctg 4501 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc 4561 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct 4621 cagacccttt tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc cgctgatcag 4681 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct 4741 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc 4801 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg 4861 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg 4921 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa 4981 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc 5041 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag 5101 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca 5161 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc 5221 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa 5281 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct 5341 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt 5401 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat 5461 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag 5521 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat 5581 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt 5641 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg 5701 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg 5761 atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac 5821 gacaaggtga ggaactaaac catggccaag ttgaccagtg ccgttccggt gctcaccgcg 5881 cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc 5941 gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc 6001 caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag 6061 ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc 6121 atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc 6181 aactgcgtgc acttcgtggc cgaggagcag gactgacacg tgctacgaga tttcgattcc 6241 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg 6301 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca 6361 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt 6421 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata 6481 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat 6541 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg 6601 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag 6661 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt 6721 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg 6781 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg 6841 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag 6901 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga 6961 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct 7021 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc 7081 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg 7141 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc 7201 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca 7261 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag 7321 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct 7381 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc 7441 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga 7501 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca 7561 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat 7621 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac 7681 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt 7741 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt 7801 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag 7861 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct 7921 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt 7981 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc 8041 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt 8101 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg 8161 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg 8221 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct 8281 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc 8341 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt 8401 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt 8461 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg 8521 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat 8581 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg 8641 cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc 8701 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc 8761 ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc 8821 aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc 8881 gcgatgtacg ggccagatat acgcgttgac attgattatt gactagttat taatagtaat 8941 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg 9001 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt 9061 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac 9121 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg 9181 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact 9241 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt 9301 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc 9361 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc 9421 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata 9481 taagcagcgc gttttgcctg tactgggtct ctctggttag accagatctg agcctgggag 9541 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt 9601 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt 9661 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga 9721 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg 9781 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag 9841 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc 9901 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg 9961 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa 10021 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata 10081 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag 10141 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg 10201 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa 10261 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg 10321 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca 10381 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg 10441 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg 10501 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga 10561 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc 10621 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac 10681 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta 10741 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa 10801 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc 10861 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg 10921 aatagagtta ggcagggata ttc //