LOCUS Exported 12140 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pLKO-puro FLAG SREBP1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12140) AUTHORS Peterson TR, Sengupta SS, Harris TE, Carmack AE, Kang SA, Balderas E, Guertin DA, Madden KL, Carpenter AE, Finck BN, Sabatini DM TITLE mTOR Complex 1 Regulates Lipin 1 Localization to Control the SREBP Pathway. JOURNAL Cell. 2011 Aug 5;146(3):408-20. PUBMED 21816276 REFERENCE 2 (bases 1 to 12140) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..12140 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 107..128 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 143..173 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 181..197 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 186..208 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 205..221 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 205..221 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 240..260 /label=T3 /note="T3 promoter, forward primer" promoter 242..260 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" promoter 288..514 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" LTR 515..695 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 742..867 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1364..1597 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 3120..3164 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" promoter 3471..3674 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 3625..3645 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 3671..3695 /label=LNCX /note="Human CMV promoter, forward primer" promoter 3905..3923 /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind 3905..3922 /label=SP6 /note="SP6 promoter, forward primer" primer_bind 3936..3955 /label=pMT2-F /note="Synthetic intron, forward primer" CDS 4014..4037 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" misc_feature 7521..7638 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 7687..8197 /label=hPGK promoter /note="human phosphoglycerate kinase 1 promoter" primer_bind 8116..8134 /label=hPGK-F /note="Human PGK promoter, forward primer" CDS 8219..8818 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(8219..8238) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 8715..8735 /label=Puro-F /note="Puromycin resistance gene, forward primer" LTR 8946..9179 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 9251..9372 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(9288..9307) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 9342..9361 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 9412..9547 /label=SV40 ori /note="SV40 origin of replication" primer_bind 9474..9493 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind complement(9567..9586) /label=T7 /note="T7 promoter, forward primer" promoter complement(9568..9586) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(9596..9613) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(9596..9612) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(9605..9627) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 9754..10209 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(9841..9860) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 10051..10072 /label=F1ori-F /note="F1 origin, forward primer" promoter 10235..10339 /gene="bla" /label=AmpR promoter CDS 10340..11200 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(10558..10577) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 11371..11959 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 11860..11879 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 12113..12130 /label=L4440 /note="L4440 vector, forward primer" ORIGIN 1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg 241 caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc 301 aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga 361 gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg 421 aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga 481 tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg 541 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc 601 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct 661 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa 721 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg 781 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag 841 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg 901 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg 961 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc 1021 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga 1081 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac 1141 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag 1201 caagcggccg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa 1261 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg 1321 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg 1381 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg 1441 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg 1501 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc 1561 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa 1621 aactcatttg caccactgct gtgccctgat gaatccccta atgattttta tcaaaatcat 1681 taagttaagg tagatacaca tcttgtcata tgatcaaatg gtttcgccaa aaatcaataa 1741 tcagacaaca aaatgtgcga actcgatatt ttacacgact ctctttacca attctgcccc 1801 gaattacact taaaacgact caacagctta acgttggctt gccacgcctt acttgactgt 1861 aaaactctca ctcttaccga acttggccgt aacctgccaa ccaaagcgag aacaaaacat 1921 aacatcaaac gaatcgaccg attgttaggt aatcgtcacc tccacaaaga gcgactcgct 1981 gtataccgtt ggcatgctag ctttatctgt tcgggcaata cgatgcccat tgtacttgtt 2041 gactggtctg atatccgtga gcaaaaacgg cttatggtat tgcgagcttc agtcgcacta 2101 cacggtcgtt ctgttactct ttatgagaaa gcgttcccgc tttcagagca atgttcaaag 2161 aaagctcatg accaatttct agccgacctt gcgagcattc taccgagtaa caccacaccg 2221 ctcattgtca gtgatgctgg ctttaaagtg ccatggtata aatccgttga gaagctgggt 2281 tggtactggt taagtcgagt aagaggaaaa gtacaatatg cagacctagg agcggaaaac 2341 tggaaaccta tcagcaactt acatgatatg tcatctagtc actcaaagac tttaggctat 2401 aagaggctga ctaaaagcaa tccaatctca tgccaaattc tattgtataa atctcgctct 2461 aaaggccgaa aaaatcagcg ctcgacacgg actcattgtc accacccgtc acctaaaatc 2521 tactcagcgt cggcaaagga gccatggatt ctagcaacta acttacctgt tgaaattcga 2581 acacccaaac aacttgttaa tatctattcg aagcgaatgc agattgaaga aaccttccga 2641 gacttgaaaa gtcctgccta cggactaggc ctacgccata gccgaacgag cagctcagag 2701 cgttttgata tcatgctgct aatcgccctg atgcttcaac taacatgttg gcttgcgggc 2761 gttcatgctc agaaacaagg ttgggacaag cacttccagg ctaacacagt cagaaatcga 2821 aacgtactct caacagttcg cttaggcatg gaagttttgc ggcattctgg ctacacaata 2881 acaagggaag acttactcgt ggctgcaacc ctactagctc aaaatttatt cacacatggt 2941 tacgctttgg ggaaattatg aggggatctc tcagtgctgt gccttggaat gctagttgga 3001 gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa 3061 ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa 3121 agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca 3181 taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt 3241 taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat 3301 tatcgtttca gacccacctc ccaaccccga ggggacccca tatgccaagt acgcccccta 3361 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg 3421 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt 3481 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc 3541 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat 3601 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct 3661 atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt 3721 ttgacctcca tagaagacac cgggaccgat ccagcctccg cggccgggaa cggtgcattg 3781 gaacgcggat tccccgtgcc aagagtgacg taagtaccgc ctatagagtc tataggccca 3841 cccccttggc ttcgttagaa cgcggctaca attaatacat aaccttatgt atcatacaca 3901 tacgatttag gtgacactat agaataacat ccactttgcc tttctctcca caggtgtcca 3961 ctcccaggtc caactgcacc tcggttctat cgattgaatt caccatggaa atggactaca 4021 aggatgacga tgacaagggt ggtggtggtg gtgcgtcgac gatggacgag ctggccttcg 4081 gtgaggcggc tctggaacag acactggccg agatgtgcga actggacaca gcggttttga 4141 acgacatcga agacatgctc cagctcatca acaaccaaga cagtgacttc ccgggcctgt 4201 ttgacgcccc ctatgctggg ggtgagacag gggacacagg ccccagcagc ccaggtgcca 4261 actctcctga gagcttctct tctgcttctc tggcctcctc tctggaagcc ttcctgggag 4321 gacccaaggt gacacctgca cccttgtccc ctccaccatc ggcacccgct gctttaaaga 4381 tgtacccgtc cgtgtccccc ttttcccctg ggcctgggat caaagaggag ccagtgccac 4441 tcaccatcct acagcctgca gcgccacagc cgtcaccggg gaccctcctg cctccgagct 4501 tccccgcacc acccgtacag ctcagccctg cgcccgtgct gggttactcg agcctgcctt 4561 caggcttctc agggaccctt ccaggaaaca ctcagcagcc accatctagc ctgccgctgg 4621 cccctgcacc aggagtcttg cccacccctg ccctgcacac ccaggtccaa agcttggcct 4681 cccagcagcc gctgccagcc tcagcagccc ctagaacaaa cactgtgacc tcacaggtcc 4741 agcaggtccc agttgtactg cagccacact tcatcaaggc agactcactg ctgctgacag 4801 ctgtgaagac agatgcagga gccaccatga agactgcagg catcagcacc ctggctcctg 4861 gcacagccgt gcaggcaggt cccctgcaga ccctggtgag tggagggacc atcttggcca 4921 cagtaccttt ggttgtggac acagacaaac tgcccatcca ccgactcgca gctggcagca 4981 aggccctagg ctcagctcag agccgtggtg agaagcgcac agcccacaat gccattgaga 5041 agcgctaccg gtcctctatc aatgacaaga ttgtggagct caaagacctg gtggtgggca 5101 ctgaagcaaa gctgaataaa tctgctgtct tgcgcaaggc catcgactac atccgcttct 5161 tgcagcacag caaccagaag ctcaagcagg agaacctgac cctacgaagt gcacacaaaa 5221 gcaaatcact gaaggacctg gtgtcagctt gtggcagtgg aggaggcaca gatgtgtcta 5281 tggagggcat gaaacccgaa gtggtggaga cgcttacccc tccaccctca gacgccggct 5341 caccctccca gagtagcccc ttgtcttttg gcagcagagc tagcagcagt ggtggtagtg 5401 actctgagcc cgacagtcca gcctttgagg atagccaggt caaagcccag cggctgcctt 5461 cacacagccg aggcatgctg gaccgctccc gcctggccct gtgtgtactg gcctttctgt 5521 gtctgacctg caatcctttg gcctcgcttt tcggctgggg cattctcact ccctctgatg 5581 ctacgggtac acaccgtagt tctgggcgca gcatgctgga ggcagagagc agagatggct 5641 ctaattggac ccagtggttg ctgccacccc tagtctggct ggccaatgga ctactagtgt 5701 tggcctgctt ggctcttctc tttgtctatg gggaacctgt gactaggcca cactctggcc 5761 cggctgtaca cttctggaga catcgcaaac aagctgacct ggatttggcc cggggagatt 5821 tcccccaggc tgctcaacag ctgtggctgg ccctgcaagc gctgggccgg cccctgccca 5881 cctcaaacct ggatctggcc tgcagtctgc tttggaacct catccgccac ctgctccagc 5941 gtctctgggt gggccgctgg ctggcaggcc aggccggggg cctgctgagg gaccgtgggc 6001 tgaggaagga tgcccgtgcc agtgcccggg atgcggctgt tgtctaccat aagctgcacc 6061 agctgcatgc catgggcaag tacacaggag gacatcttgc tgcttctaac ctggcactaa 6121 gtgccctcaa cctggctgag tgcgcaggag atgctatctc catggcaaca ctggcagaga 6181 tctatgtggc agcggccctg agggtcaaaa ccagcctccc aagagccctg cacttcttga 6241 cacgtttctt cctgagcagc gcccgccagg cctgcctagc acagagcggc tcggtgcctc 6301 ttgccatgca gtggctctgc caccctgtag gtcaccgttt ctttgtggac ggggactggg 6361 ccgtgcacgg tgcccccccg gagagcctgt acagcgtggc tgggaaccca gtggatccgc 6421 tggcccaggt gacccggcta ttccgtgaac atctcctaga gcgagcgttg aactgtattg 6481 ctcagcccag cccaggggca gctgacggag acagggagtt ctcagatgcc cttggatatc 6541 tgcagttgct aaatagctgt tctgatgctg ccggggctcc tgcttgcagt ttctctgtca 6601 gctccagcat ggctgccacc actggcccag acccagtggc caagtggtgg gcctcactga 6661 cagctgtggt gatccactgg ctgaggcggg atgaagaggc agctgagcgc ttgtacccac 6721 tggtagagca tatcccccag gtgctgcagg acactgagag acccctgccc agggcagctc 6781 tgtactcctt caaggctgcc cgggctctgc tggaccacag aaaggtggaa tctagcccag 6841 ccagcctggc catctgtgag aaggccagtg ggtacctgcg ggacagctta gcctctacac 6901 caactggcag ttccattgac aaggccatgc agctgctcct gtgtgatcta cttcttgtgg 6961 cccgtaccag tctgtggcag cggcagcagt caccagcttc agtccaggta gctcacggta 7021 ccagcaatgg accccaggcc tctgctctgg agctgcgtgg tttccaacat gacctgagca 7081 gcctgcggcg gttggcacag agcttccggc ctgctatgag gagggtattc ctacatgagg 7141 ccacagctcg gctgatggca ggagcaagtc ctgcccggac acaccagctc ctggatcgca 7201 gtctgaggag gagggcaggt tccagtggca aaggaggcac tacagctgag ctggagccac 7261 ggcccacatg gcgggagcac accgaggccc tgctgttggc atcctgctat ctgccccctg 7321 ccttcctgtc ggctcctggg cagcgaatga gcatgctggc cgaggcggca cgcaccgtag 7381 agaagcttgg cgatcaccgg ctactgctgg actgccagca gatgctcctg cgcctgggcg 7441 gcggaaccac cgtcacttcc agctaggcgg ccgcagtgag aattctcgac ctcgagacaa 7501 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg 7561 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt 7621 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccact ttggccgcgg 7681 ctcgaggggg ttggggttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg 7741 ctgctctggg cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt 7801 cacgtccgtt cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac 7861 gcttcctgct ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg 7921 acaaacggaa gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat 7981 ggcagcgcgc cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga 8041 gagcagcggc cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct 8101 gttcctgccc gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg 8161 ctccctcgtt gaccgaatca ccgacctctc tccccagggg gatccaccgg agcttaccat 8221 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg 8281 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg 8341 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat 8401 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag 8461 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc 8521 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc 8581 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag 8641 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga 8701 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga 8761 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacg 8821 cccgccccac gacccgcagc gcccgaccga aaggagcgca cgaccccatg catcggtacc 8881 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg 8941 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg 9001 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact 9061 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg 9121 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag 9181 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga 9241 gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac 9301 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat 9361 caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc 9421 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat 9481 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt 9541 ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg 9601 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg 9661 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt 9721 cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg 9781 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg 9841 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc 9901 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa 9961 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc 10021 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac 10081 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt 10141 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc 10201 ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt 10261 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata 10321 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt 10381 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc 10441 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat 10501 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct 10561 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca 10621 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg 10681 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa 10741 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg 10801 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga 10861 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg 10921 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt 10981 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg 11041 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc 11101 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca 11161 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc 11221 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat 11281 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc 11341 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg 11401 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct 11461 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct 11521 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct 11581 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg 11641 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc 11701 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga 11761 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg 11821 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta 11881 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg 11941 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg 12001 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat 12061 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc 12121 agtgagcgag gaagcggaag //