LOCUS       Exported               10697 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  parental, all-in-one CRISPRi vector for B. burgdorferi, parental for
            gRNA cloning.
ACCESSION   .
VERSION     .
KEYWORDS    pBbdCas9S(-10TC)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10697)
  AUTHORS   Takacs CN, Scott M, Chang Y, Kloos ZA, Irnov I, Rosa PA, Liu J, 
            Jacobs-Wagner C
  TITLE     A CRISPR interference platform for selective downregulation of gene 
            expression in Borrelia burgdorferi.
  JOURNAL   Appl Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20.
  PUBMED    33257311
REFERENCE   2  (bases 1 to 10697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10697
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        4737..4781
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    4757..4773
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    4789..4805
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             4824..4835
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             4842..8948
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             9118..9909
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      10032..10620
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10521..10540
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tccccggcaa gttcatcctt agggtttcca tatctcttaa aaagtcaatt tctgcttgat
       61 taatttcttg tggattattg tttttgcggt ttttcatttt ttatctcctt gagtattgaa
      121 ttttttctta tatataaata tatagcaaaa actatttttg ccaacttttt tacaaaaaat
      181 tttttattaa aacacttagg gctttgctaa attctctttt aaaagaactt agtaaagccc
      241 tatggattta agaactgatt atacttacgt agtaaaaata ctacagatat taatagtata
      301 gaccatatca aaatcataat caatttatat ttatctaaaa ttgtagtcca tacaaaatac
      361 aaaaaaatta ggtttttcta aattctattt taaaagaact tagtaacacc taatctctaa
      421 attacttaca aaattctaaa attagatttt tagttcttca tatttcttta aaagtttttt
      481 aagaaaatct ttttgatttt caaaaatttc atgcatcata aaactagtaa atttggattt
      541 ttttttgtaa aaatcataac tttcttgggt tttaagttga aatcttaatg gttttattgg
      601 attttgccta gattttttta acgccggact ttctttatct tttataaaat tataagaatt
      661 tttaatacca ttttctatta aataatcttc ttttataagc ccctcttgca gtgcagttgc
      721 taatcttaaa tacttatacg cttgagtttt tgctacatca aagtctttta taaaatcaag
      781 aaaagtttta taaccatcaa atttataata ttgattatct tttatttctt ttaaaatttt
      841 aatattttcc accctgtgga atatttcttt tttgaaattg tcatttaact tttcctttag
      901 tgtcttgtaa taagaaattt tactttcatt aatcattaaa ttttgttcaa cataagtatc
      961 tatctttttt gttaaaactt taacaacacg ttttttttcc ataaaaattt tcctttattt
     1021 ttgcaacatt ttccttaatt gatataattg ttactatttt agatttttta atatccactt
     1081 aagaattaat ccctcactag aatatttttt attatagaat tcatatacat gtttttctaa
     1141 atctataaat ttatcaacaa ttgctttatt atagggtgta tctatctttt cttttcttaa
     1201 taatcgtctc atattcttta aataacaaca aacactgccg gttttaaacc taaattctat
     1261 gtaatatatt tttgaaaaag tatatacatt ttcaaccccg tttatttcat actttacaaa
     1321 aacattattt attggttttc tataaccata gtaaatgcct aaaaatttgt ccccgtcttt
     1381 cataggatat agataaagtt tttgaatctc tgatttatct aataattttt caaaaaaaat
     1441 tatacaccgc ttttgtttgt tactaactac tctatgcaat gcctttaata tttttgtgtg
     1501 atatatattt ttatcacctt ttttttcttt cagcaggaag atactttctt ttggaaattt
     1561 aattttttgt tcatttattc ttgcagcctt aagtccgatt ttcatattta atccttatat
     1621 agcctttcta tttaattctt ttaaactcaa taaacttttt tggtttttca ttagttctaa
     1681 taattcgcaa taatatgtgc aaaacacttt attgtattct attttctttt ggttattcaa
     1741 atattctttt ataattggct ttagaagttc aatatttact tcttttttta gttgttcaat
     1801 aaggatattg aaaatgtttg tttttaaatc ttgataattt tgttgagaat tttctttttt
     1861 tctttcaatt gacttctcta atttacgctt tatattgttt aaatcgctat atttatgact
     1921 ttcaataata aaatgaggct taaatttgta attttcgtat attttttgga aacttgtttc
     1981 taattgttta gaattatatt catttttttc aaattctttt tgagtgttgc atagaatttc
     2041 ctttaatttt tcttgttttt ctttcataca agatttagta aaatgtatat ttttattttt
     2101 tagtaattta atttctatta tttttatgat tttaagtatt ttgattatat tatctttatc
     2161 aatatttaaa ttgaataaaa ttgtaagaaa aattgaaaga atttttttac ataaaaagtt
     2221 acatttattg aagtaatttc ttacttgata cttttctatt tcattaatct ttctttcttc
     2281 ttttatatta tttttattat ttaaacactc ctttaaatct acactcccat ttttagtaaa
     2341 tctatcttta aggccccttg aaactctatt ttgaaatctt aaatcttttt tttcttgaaa
     2401 gtatttatag attttttggt aacattcttt ttttgaaaaa ttaagttgat agtatatttc
     2461 ggtccccaaa tttaccccta agtgtttgtg ataattagtt gtgactttaa attctttttc
     2521 taatttgtaa aggtaatttt gcagtgtttt tagtttaacg ggtgtttgac tatttctttt
     2581 aaggttttca ttaaagtaat agagtatgtt tttttgtgtg tatttcttat atgttttatt
     2641 tatgtacgct agtgttgaga taagaactac taatttatac tgtaatttat tgtagcaagt
     2701 tgtggtttta ctggttttgt ttatattaga taagttttcc atttatagct ccttgaatac
     2761 cggatttttt atctataaag tatatatatc acaattttga tttaaaagta aattcttttt
     2821 ttaaaaatac aaaaattttt tataaattat atagaccatg atattgtatt aatactaatt
     2881 gattttaatt taaggttgaa ctaaaataaa catagttttg tagaaaataa tttttcatta
     2941 ttttgctact tgatattgga tcgtaaaaat attagggctt tacaaagttc ttttaaaaga
     3001 gaatttagta aagccctaag tcttttaata aaaaattttt tgtaaaaaag ttggcaaaaa
     3061 tagtttttgc tatatattta tatataagaa aaaattcaat actcaaggag ataaaaaatg
     3121 gaaaacttaa acaataatcc acaaaaaatt aatcaaataa ataagcaaac taattttatt
     3181 ggttttgaaa attttataga acttgataat caagttaatc aaactaaaga aaaaagtaaa
     3241 ttaagtaaga taggtaagaa attacccgga ataagcagtc aagtctagtc tagtgccggc
     3301 cggcgcgcca aataatgccc gtgtccctgt tgaataggga cacggttttt taacaatttt
     3361 tttattccta ggccttagtg gccggtaccc ggggatcctt attactggcc gctttctagc
     3421 ctggatactt gtctggctag ttgcattaga gagtcagcta gagccctagg gctagctgtt
     3481 tgtgtattcg gagccagtgt agtttttctt tttactaacg atacgggtaa taattgattg
     3541 ccttttacag cctgaccttg agacaattgc aaaagtctgt ccacagaggt ttgacctaat
     3601 aatctaaaat cttgtttgat agtagtaagt ggaggtatgt agcaactaga atcttctgta
     3661 tcatcataac ccacaactga aatatcagcc ccaactctaa gtccactttc agtaatagct
     3721 cgcatagctc ctaaagccat ttgatcatta gcaactaaca tagcagtagg gacaatgcct
     3781 tcatttaaca tttgcattgt ttgctgaaat cctgacatag cagaccaatc tccttctctt
     3841 tctgctattg gttgtatttg atttcttgtt aaatacttgt gccatcctgc taatctcaat
     3901 ctggcactta ctgaacttaa gggacctgca agaagagcaa tttgttgatg gcctaatgca
     3961 acaaggtgtt caactcctaa ccttgttcca tcttcgtgag aaaaaattat agagtttatt
     4021 ggggtttggt ccgacacatc caaaaacaat gctggaacat tagtacatgc tgcttcaact
     4081 gcaatcgcat cttggtcatc aagaggataa ttaattataa gtccactaac acgttgagct
     4141 aataaattat gtactgctgc cttacaagcc tctacacctg atctctcaac cattgataca
     4201 acaacgcttg caccaagttg atcagctctc gatttaattg cggctacaat ttgactaggc
     4261 gcatgtaatg caagtgatga tgtggcaact ccaattaaaa gtgattgttt ccccgcaagt
     4321 tgctgagcaa cgcgatttgg tatataattc aactctgcca tagctgcttc aaccttctct
     4381 cttgtttttg cagatacatg acttgcttgg ttcacaactc tcgaaaccgt ttggtagcta
     4441 accccggcat attcagcaac atcatacaaa gtaacaggtt tcatggatca ttcctccatg
     4501 ataaaattta aatttctgac tttggcaaat ccttttgcct gttaaaaaga atagcacaaa
     4561 aattaaaaaa aaagaaacgc aaatagcaat agcattaaaa ataattatta ttatttattt
     4621 ctttgattaa aatcaaaagc ctttaatgta ttttcttgat ctttcatgcg cttaacggta
     4681 aaatccaagg tattaagttc accttccact tttctctctc tatcttctct agaaaatcat
     4741 aaaaaattta tttgctttgt gagcggataa caattataac agattcaatt gtgagcggat
     4801 aacaatttca cacagaattc attaaagagg agaaattaca tatggataag aaatactcaa
     4861 taggcttagc tatcggcaca aatagcgtcg gatgggcggt gatcactgat gaatataagg
     4921 ttccgtctaa aaagttcaag gttctgggaa atacagaccg ccacagtatc aaaaaaaatc
     4981 ttataggggc tcttttattt gacagtggag agacagcgga agcgactcgt ctcaaacgga
     5041 cagctcgtag aaggtataca cgtcggaaga atcgtatttg ttatctacag gagatttttt
     5101 caaatgagat ggcgaaagta gatgatagtt tctttcatcg acttgaagag tcttttttgg
     5161 tggaagaaga caagaagcat gaacgtcatc ctatttttgg aaatatagta gatgaagttg
     5221 cttatcatga gaaatatcca actatctatc atctgcgaaa aaaattggta gattctactg
     5281 ataaagcgga tttgcgctta atctatttgg ccttagcgca tatgattaag tttcgtggtc
     5341 attttttgat tgagggagat ttaaatcctg ataatagtga tgtggacaaa ctatttatcc
     5401 agttggtaca aacctacaat caattatttg aagaaaaccc tattaacgca agtggagtag
     5461 atgctaaagc gattctttct gcacgattga gtaaatcaag acgattagaa aatctcattg
     5521 ctcagctccc cggtgagaag aaaaatggct tatttgggaa tctcattgct ttgtcattgg
     5581 gtttgacccc taattttaaa tcaaattttg atttggcaga agatgctaaa ttacagcttt
     5641 caaaagatac ttacgatgat gatttagata atttattggc gcaaattgga gatcaatatg
     5701 ctgatttgtt tttggcagct aagaatttat cagatgctat tttactttca gatatcctaa
     5761 gagtaaatac tgaaataact aaggctcccc tatcagcttc aatgattaaa cgctacgatg
     5821 aacatcatca agacttgact cttttaaaag ctttagttcg acaacaactt ccagaaaagt
     5881 ataaagaaat cttttttgat caatcaaaaa acggatatgc aggttatatt gatgggggag
     5941 ctagccaaga agaattttat aaatttatca aaccaatttt agaaaaaatg gatggtactg
     6001 aggaattatt ggtgaaacta aatcgtgaag atttgctgcg caagcaacgg acctttgaca
     6061 acggctctat tccccatcaa attcacttgg gtgagctgca tgctattttg agaagacaag
     6121 aagactttta tccattttta aaagacaatc gtgagaagat tgaaaaaatc ttgacttttc
     6181 gaattcctta ttatgttggt ccattggcgc gtggcaatag tcgttttgca tggatgactc
     6241 ggaagtctga agaaacaatt accccatgga attttgaaga agttgtcgat aaaggtgctt
     6301 cagctcaatc atttattgaa cgcatgacaa actttgataa aaatcttcca aatgaaaaag
     6361 tactaccaaa acatagtttg ctttatgagt attttacggt ttataacgaa ttgacaaagg
     6421 tcaaatatgt tactgaagga atgcgaaaac cagcatttct ttcaggtgaa cagaagaaag
     6481 ccattgttga tttactcttc aaaacaaatc gaaaagtaac cgttaagcaa ttaaaagaag
     6541 attatttcaa aaaaatagaa tgttttgata gtgttgaaat ttcaggagtt gaagatagat
     6601 ttaatgcttc attaggtacc taccatgatt tgctaaaaat tattaaagat aaagattttt
     6661 tggataatga agaaaatgaa gatatcttag aggatattgt tttaacattg accttatttg
     6721 aagataggga gatgattgag gaaagactta aaacatatgc tcacctcttt gatgataagg
     6781 tgatgaaaca gcttaaacgt cgccgttata ctggttgggg acgtttgtct cgaaaattga
     6841 ttaatggtat tagggataag caatctggca aaacaatatt agattttttg aaatcagatg
     6901 gttttgccaa tcgcaatttt atgcagctga tccatgatga tagtttgaca tttaaagaag
     6961 acattcaaaa agcacaagtg tctggacaag gcgatagttt acatgaacat attgcaaatt
     7021 tagctggtag ccctgctatt aaaaaaggta ttttacagac tgtaaaagtt gttgatgaat
     7081 tggtcaaagt aatggggcgg cataagccag aaaatatcgt tattgaaatg gcacgtgaaa
     7141 atcagacaac tcaaaagggc cagaaaaatt cgcgagagcg tatgaaacga atcgaagaag
     7201 gtatcaaaga attaggaagt cagattctta aagagcatcc tgttgaaaat actcaattgc
     7261 aaaatgaaaa gctctatctc tattatctcc aaaatggaag agacatgtat gtggaccaag
     7321 aattagatat taatcgttta agtgattatg atgtcgatgc cattgttcca caaagtttcc
     7381 ttaaagacga ttcaatagac aataaggtct taacgcgttc tgataaaaat cgtggtaaat
     7441 cggataacgt tccaagtgaa gaagtagtca aaaagatgaa aaactattgg agacaacttc
     7501 taaacgccaa gttaatcact caacgtaagt ttgataattt aacgaaagct gaacgtggag
     7561 gtttgagtga acttgataaa gctggtttta tcaaacgcca attggttgaa actcgccaaa
     7621 tcactaagca tgtggcacaa attttggata gtcgcatgaa tactaaatac gatgaaaatg
     7681 ataaacttat tcgagaggtt aaagtgatta ccttaaaatc taaattagtt tctgacttcc
     7741 gaaaagattt ccaattctat aaagtacgtg agattaacaa ttaccatcat gcccatgatg
     7801 cgtatctaaa tgccgtcgtt ggaactgctt tgattaagaa atatccaaaa cttgaatcgg
     7861 agtttgtcta tggtgattat aaagtttatg atgttcgtaa aatgattgct aagtctgagc
     7921 aagaaatagg caaagcaacc gcaaaatatt tcttttactc taatatcatg aacttcttca
     7981 aaacagaaat tacacttgca aatggagaga ttcgcaaacg ccctctaatc gaaactaatg
     8041 gggaaactgg agaaattgtc tgggataaag ggcgagattt tgccacagtg cgcaaagtat
     8101 tgtccatgcc ccaagtcaat attgtcaaga aaacagaagt acagacaggc ggattctcca
     8161 aggagtcaat tttaccaaaa agaaattcgg acaagcttat tgctcgtaaa aaagactggg
     8221 atccaaaaaa atatggtggt tttgatagtc caacggtagc ttattcagtc ctagtggttg
     8281 ctaaggtgga aaaagggaaa tcgaagaagt taaaatccgt taaagagtta ctagggatca
     8341 caattatgga aagaagttcc tttgaaaaaa atccgattga ctttttagaa gctaaaggat
     8401 ataaggaagt taaaaaagac ttaatcatta aactacctaa atatagtctt tttgagttag
     8461 aaaacggtcg taaacggatg ctggctagtg ccggagaatt acaaaaagga aatgagctgg
     8521 ctctgccaag caaatatgtg aattttttat atttagctag tcattatgaa aagttgaagg
     8581 gtagtccaga agataacgaa caaaaacaat tgtttgtgga gcagcataag cattatttag
     8641 atgagattat tgagcaaatc agtgaatttt ctaagcgtgt tattttagca gatgccaatt
     8701 tagataaagt tcttagtgca tataacaaac atagagacaa accaatacgt gaacaagcag
     8761 aaaatattat tcatttattt acgttgacga atcttggagc tcccgctgct tttaaatatt
     8821 ttgatacaac aattgatcgt aaacgatata cgtctacaaa agaagtttta gatgccactc
     8881 ttatccatca atccatcact ggtctttatg aaacacgcat tgatttgagt cagctaggag
     8941 gtgactaata aaagcttgat cagatctcag cttttttttg aagtgcctgg cagtaagttg
     9001 aaaaaatttg aaataaactt ggactatgtt aataaaaaaa tacaaattaa cgaacaaaaa
     9061 gatttgaatt attatataat ttagttaaaa gcaattttaa atgagggagg tttccatatg
     9121 agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag
     9181 cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc
     9241 ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca
     9301 acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag
     9361 attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat
     9421 ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc
     9481 ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat
     9541 agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat
     9601 ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc
     9661 gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa
     9721 atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag
     9781 cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg
     9841 cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc
     9901 ggcaaataat gacgtcgccg gagcggtcga gttctggacc gaccggctcg ggttctcatg
     9961 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
    10021 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
    10081 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
    10141 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
    10201 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
    10261 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
    10321 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
    10381 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
    10441 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
    10501 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
    10561 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
    10621 aacgccagca acgcggcctt tttacggttc ctgggctttt gctggccttt tgctcacatg
    10681 ttctttcctg cactaga
//
