LOCUS Exported 11605 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning. ACCESSION . VERSION . KEYWORDS pBbdCas9H(RBSmut)_arr2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11605) AUTHORS Takacs CN, Scott M, Chang Y, Kloos ZA, Irnov I, Rosa PA, Liu J, Jacobs-Wagner C TITLE A CRISPR interference platform for selective downregulation of gene expression in Borrelia burgdorferi. JOURNAL Appl Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 10.1128/AEM.02519-20. PUBMED 33257311 REFERENCE 2 (bases 1 to 11605) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11605 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 5371..5415 /label=T5 promoter /note="bacteriophage T5 promoter for E. coli RNA polymerase, with embedded lac operator" protein_bind 5391..5407 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." protein_bind 5423..5439 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." CDS 5476..9579 /codon_start=1 /product="catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=dCas9 /note="RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain" /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL DATLIHQSITGLYETRIDLSQLGGD" CDS 9809..10828 /codon_start=1 /product="hygromycin B phosphotransferase" /label=HygR /note="confers resistance to hygromycin" /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE" rep_origin 10951..11539 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 11440..11459 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 cctgcactag atccccggca agttcatcct tagggtttcc atatctctta aaaagtcaat 61 ttctgcttga ttaatttctt gtggattatt gtttttgcgg tttttcattt tttatctcct 121 tgagtattga attttttctt atatataaat atatagcaaa aactattttt gccaactttt 181 ttacaaaaaa ttttttatta aaacacttag ggctttgcta aattctcttt taaaagaact 241 tagtaaagcc ctatggattt aagaactgat tatacttacg tagtaaaaat actacagata 301 ttaatagtat agaccatatc aaaatcataa tcaatttata tttatctaaa attgtagtcc 361 atacaaaata caaaaaaatt aggtttttct aaattctatt ttaaaagaac ttagtaacac 421 ctaatctcta aattacttac aaaattctaa aattagattt ttagttcttc atatttcttt 481 aaaagttttt taagaaaatc tttttgattt tcaaaaattt catgcatcat aaaactagta 541 aatttggatt tttttttgta aaaatcataa ctttcttggg ttttaagttg aaatcttaat 601 ggttttattg gattttgcct agattttttt aacgccggac tttctttatc ttttataaaa 661 ttataagaat ttttaatacc attttctatt aaataatctt cttttataag cccctcttgc 721 agtgcagttg ctaatcttaa atacttatac gcttgagttt ttgctacatc aaagtctttt 781 ataaaatcaa gaaaagtttt ataaccatca aatttataat attgattatc ttttatttct 841 tttaaaattt taatattttc caccctgtgg aatatttctt ttttgaaatt gtcatttaac 901 ttttccttta gtgtcttgta ataagaaatt ttactttcat taatcattaa attttgttca 961 acataagtat ctatcttttt tgttaaaact ttaacaacac gttttttttc cataaaaatt 1021 ttcctttatt tttgcaacat tttccttaat tgatataatt gttactattt tagatttttt 1081 aatatccact taagaattaa tccctcacta gaatattttt tattatagaa ttcatataca 1141 tgtttttcta aatctataaa tttatcaaca attgctttat tatagggtgt atctatcttt 1201 tcttttctta ataatcgtct catattcttt aaataacaac aaacactgcc ggttttaaac 1261 ctaaattcta tgtaatatat ttttgaaaaa gtatatacat tttcaacccc gtttatttca 1321 tactttacaa aaacattatt tattggtttt ctataaccat agtaaatgcc taaaaatttg 1381 tccccgtctt tcataggata tagataaagt ttttgaatct ctgatttatc taataatttt 1441 tcaaaaaaaa ttatacaccg cttttgtttg ttactaacta ctctatgcaa tgcctttaat 1501 atttttgtgt gatatatatt tttatcacct tttttttctt tcagcaggaa gatactttct 1561 tttggaaatt taattttttg ttcatttatt cttgcagcct taagtccgat tttcatattt 1621 aatccttata tagcctttct atttaattct tttaaactca ataaactttt ttggtttttc 1681 attagttcta ataattcgca ataatatgtg caaaacactt tattgtattc tattttcttt 1741 tggttattca aatattcttt tataattggc tttagaagtt caatatttac ttcttttttt 1801 agttgttcaa taaggatatt gaaaatgttt gtttttaaat cttgataatt ttgttgagaa 1861 ttttcttttt ttctttcaat tgacttctct aatttacgct ttatattgtt taaatcgcta 1921 tatttatgac tttcaataat aaaatgaggc ttaaatttgt aattttcgta tattttttgg 1981 aaacttgttt ctaattgttt agaattatat tcattttttt caaattcttt ttgagtgttg 2041 catagaattt cctttaattt ttcttgtttt tctttcatac aagatttagt aaaatgtata 2101 tttttatttt ttagtaattt aatttctatt atttttatga ttttaagtat tttgattata 2161 ttatctttat caatatttaa attgaataaa attgtaagaa aaattgaaag aattttttta 2221 cataaaaagt tacatttatt gaagtaattt cttacttgat acttttctat ttcattaatc 2281 tttctttctt cttttatatt atttttatta tttaaacact cctttaaatc tacactccca 2341 tttttagtaa atctatcttt aaggcccctt gaaactctat tttgaaatct taaatctttt 2401 ttttcttgaa agtatttata gattttttgg taacattctt tttttgaaaa attaagttga 2461 tagtatattt cggtccccaa atttacccct aagtgtttgt gataattagt tgtgacttta 2521 aattcttttt ctaatttgta aaggtaattt tgcagtgttt ttagtttaac gggtgtttga 2581 ctatttcttt taaggttttc attaaagtaa tagagtatgt ttttttgtgt gtatttctta 2641 tatgttttat ttatgtacgc tagtgttgag ataagaacta ctaatttata ctgtaattta 2701 ttgtagcaag ttgtggtttt actggttttg tttatattag ataagttttc catttatagc 2761 tccttgaata ccggattttt tatctataaa gtatatatat cacaattttg atttaaaagt 2821 aaattctttt tttaaaaata caaaaatttt ttataaatta tatagaccat gatattgtat 2881 taatactaat tgattttaat ttaaggttga actaaaataa acatagtttt gtagaaaata 2941 atttttcatt attttgctac ttgatattgg atcgtaaaaa tattagggct ttacaaagtt 3001 cttttaaaag agaatttagt aaagccctaa gtcttttaat aaaaaatttt ttgtaaaaaa 3061 gttggcaaaa atagtttttg ctatatattt atatataaga aaaaattcaa tactcaagga 3121 gataaaaaat ggaaaactta aacaataatc cacaaaaaat taatcaaata aataagcaaa 3181 ctaattttat tggttttgaa aattttatag aacttgataa tcaagttaat caaactaaag 3241 aaaaaagtaa attaagtaag ataggtaaga aattacccgg aataagcagt caagtctagt 3301 ctagtgcggc cgcatggctt gttatgactg tttttttggg gtacagtcta tgcctcgggc 3361 atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga 3421 tgttacgcag cagggcagtc gccctaaaac aaagttaaac atcatgagga ggtaacgtca 3481 tggtaaaaga ttggattcct atttctcatg ataattataa acaagtacaa ggaccatttt 3541 atcatggaac taaagctaat ttagcaattg gtgatttact aactacagga tttatttctc 3601 attttgaaga tggtcgaatt cttaaacata tttatttttc agctttaatg gaaccagcag 3661 tttggggagc tgaacttgct atgtcactat ctggtcttga aggtcgtggt tatatatata 3721 tagttgaacc aacaggacca tttgaagatg atccaaatct tacaaataaa aaatttcctg 3781 gtaatccaac acaatcttat agaacttgtg aacctttaag aattgttggt gttgttgaag 3841 attgggaagg acatcctgtt gaattaataa gaggaatgtt agattcatta gaagatttaa 3901 aacgtcgtgg tttacatgtt attgaagatt aacctggcgc gccaaataat gcccgtgtcc 3961 ctgttgaata gggacacggt tttttaacaa tttttttatt cctaggcctt agtggccggt 4021 acccggggat ccttattact ggccgctttc tagcctggat acttgtctgg ctagttgcat 4081 tagagagtca gctagagccc tagggctagc tgtttgtgta ttcggagcca gtgtagtttt 4141 tctttttact aacgatacgg gtaataattg attgcctttt acagcctgac cttgagacaa 4201 ttgcaaaagt ctgtccacag aggtttgacc taataatcta aaatcttgtt tgatagtagt 4261 aagtggaggt atgtagcaac tagaatcttc tgtatcatca taacccacaa ctgaaatatc 4321 agccccaact ctaagtccac tttcagtaat agctcgcata gctcctaaag ccatttgatc 4381 attagcaact aacatagcag tagggacaat gccttcattt aacatttgca ttgtttgctg 4441 aaatcctgac atagcagacc aatctccttc tctttctgct attggttgta tttgatttct 4501 tgttaaatac ttgtgccatc ctgctaatct caatctggca cttactgaac ttaagggacc 4561 tgcaagtagt gcaatttgtt gatggcctaa tgcaacaagg tgttcaactc ctaaccttgt 4621 tccatcttcg tgagaaaaaa ttatagagtt tattggggtt tggtccgaca catccaaaaa 4681 caatgctgga acattagtac atgctgcttc aactgcaatc gcatcttggt catcaagagg 4741 ataattaatt ataagtccac taacacgttg agctaataaa ttatgtactg ctgccttaca 4801 agcctctaca cctgatctct caaccattga tacaacaacg cttgcaccaa gttgatcagc 4861 tctcgattta attgcggcta caatttgact aggcgcatgt aatgcaagtg atgatgtggc 4921 aactccaatt aaaagtgatt gtttccccgc aagttgctga gcaacgcgat ttggtatata 4981 attcaactct gccatagctg cttcaacctt ctctcttgtt tttgcagata catgacttgc 5041 ttggttcaca actctcgaaa ccgtttggta gctaaccccg gcatattcag caacatcata 5101 caaagtaaca ggtttcatgg atcattcctc catgataaaa tttaaatttc tgactttggc 5161 aaatcctttt gcctgttaaa aagaatagca caaaaattaa aaaaaaagaa acgcaaatag 5221 caatagcatt aaaaataatt attattattt atttctttga ttaaaatcaa aagcctttaa 5281 tgtattttct tgatctttca tgcgcttaac ggtaaaatcc aaggtattaa gttcaccttc 5341 cacttttctc tctctatctt ctctagaaaa tcataaaaaa tttatttgct ttgtgagcgg 5401 ataacaatta taatagattc aattgtgagc ggataacaat ttcacacaga attcattaaa 5461 gaagagaaat tacatatgga taagaaatac tcaataggct tagctatcgg cacaaatagc 5521 gtcggatggg cggtgatcac tgatgaatat aaggttccgt ctaaaaagtt caaggttctg 5581 ggaaatacag accgccacag tatcaaaaaa aatcttatag gggctctttt atttgacagt 5641 ggagagacag cggaagcgac tcgtctcaaa cggacagctc gtagaaggta tacacgtcgg 5701 aagaatcgta tttgttatct acaggagatt ttttcaaatg agatggcgaa agtagatgat 5761 agtttctttc atcgacttga agagtctttt ttggtggaag aagacaagaa gcatgaacgt 5821 catcctattt ttggaaatat agtagatgaa gttgcttatc atgagaaata tccaactatc 5881 tatcatctgc gaaaaaaatt ggtagattct actgataaag cggatttgcg cttaatctat 5941 ttggccttag cgcatatgat taagtttcgt ggtcattttt tgattgaggg agatttaaat 6001 cctgataata gtgatgtgga caaactattt atccagttgg tacaaaccta caatcaatta 6061 tttgaagaaa accctattaa cgcaagtgga gtagatgcta aagcgattct ttctgcacga 6121 ttgagtaaat caagacgatt agaaaatctc attgctcagc tccccggtga gaagaaaaat 6181 ggcttatttg ggaatctcat tgctttgtca ttgggtttga cccctaattt taaatcaaat 6241 tttgatttgg cagaagatgc taaattacag ctttcaaaag atacttacga tgatgattta 6301 gataatttat tggcgcaaat tggagatcaa tatgctgatt tgtttttggc agctaagaat 6361 ttatcagatg ctattttact ttcagatatc ctaagagtaa atactgaaat aactaaggct 6421 cccctatcag cttcaatgat taaacgctac gatgaacatc atcaagactt gactctttta 6481 aaagctttag ttcgacaaca acttccagaa aagtataaag aaatcttttt tgatcaatca 6541 aaaaacggat atgcaggtta tattgatggg ggagctagcc aagaagaatt ttataaattt 6601 atcaaaccaa ttttagaaaa aatggatggt actgaggaat tattggtgaa actaaatcgt 6661 gaagatttgc tgcgcaagca acggaccttt gacaacggct ctattcccca tcaaattcac 6721 ttgggtgagc tgcatgctat tttgagaaga caagaagact tttatccatt tttaaaagac 6781 aatcgtgaga agattgaaaa aatcttgact tttcgaattc cttattatgt tggtccattg 6841 gcgcgtggca atagtcgttt tgcatggatg actcggaagt ctgaagaaac aattacccca 6901 tggaattttg aagaagttgt cgataaaggt gcttcagctc aatcatttat tgaacgcatg 6961 acaaactttg ataaaaatct tccaaatgaa aaagtactac caaaacatag tttgctttat 7021 gagtatttta cggtttataa cgaattgaca aaggtcaaat atgttactga aggaatgcga 7081 aaaccagcat ttctttcagg tgaacagaag aaagccattg ttgatttact cttcaaaaca 7141 aatcgaaaag taaccgttaa gcaattaaaa gaagattatt tcaaaaaaat agaatgtttt 7201 gatagtgttg aaatttcagg agttgaagat agatttaatg cttcattagg tacctaccat 7261 gatttgctaa aaattattaa agataaagat tttttggata atgaagaaaa tgaagatatc 7321 ttagaggata ttgttttaac attgacctta tttgaagata gggagatgat tgaggaaaga 7381 cttaaaacat atgctcacct ctttgatgat aaggtgatga aacagcttaa acgtcgccgt 7441 tatactggtt ggggacgttt gtctcgaaaa ttgattaatg gtattaggga taagcaatct 7501 ggcaaaacaa tattagattt tttgaaatca gatggttttg ccaatcgcaa ttttatgcag 7561 ctgatccatg atgatagttt gacatttaaa gaagacattc aaaaagcaca agtgtctgga 7621 caaggcgata gtttacatga acatattgca aatttagctg gtagccctgc tattaaaaaa 7681 ggtattttac agactgtaaa agttgttgat gaattggtca aagtaatggg gcggcataag 7741 ccagaaaata tcgttattga aatggcacgt gaaaatcaga caactcaaaa gggccagaaa 7801 aattcgcgag agcgtatgaa acgaatcgaa gaaggtatca aagaattagg aagtcagatt 7861 cttaaagagc atcctgttga aaatactcaa ttgcaaaatg aaaagctcta tctctattat 7921 ctccaaaatg gaagagacat gtatgtggac caagaattag atattaatcg tttaagtgat 7981 tatgatgtcg atgccattgt tccacaaagt ttccttaaag acgattcaat agacaataag 8041 gtcttaacgc gttctgataa aaatcgtggt aaatcggata acgttccaag tgaagaagta 8101 gtcaaaaaga tgaaaaacta ttggagacaa cttctaaacg ccaagttaat cactcaacgt 8161 aagtttgata atttaacgaa agctgaacgt ggaggtttga gtgaacttga taaagctggt 8221 tttatcaaac gccaattggt tgaaactcgc caaatcacta agcatgtggc acaaattttg 8281 gatagtcgca tgaatactaa atacgatgaa aatgataaac ttattcgaga ggttaaagtg 8341 attaccttaa aatctaaatt agtttctgac ttccgaaaag atttccaatt ctataaagta 8401 cgtgagatta acaattacca tcatgcccat gatgcgtatc taaatgccgt cgttggaact 8461 gctttgatta agaaatatcc aaaacttgaa tcggagtttg tctatggtga ttataaagtt 8521 tatgatgttc gtaaaatgat tgctaagtct gagcaagaaa taggcaaagc aaccgcaaaa 8581 tatttctttt actctaatat catgaacttc ttcaaaacag aaattacact tgcaaatgga 8641 gagattcgca aacgccctct aatcgaaact aatggggaaa ctggagaaat tgtctgggat 8701 aaagggcgag attttgccac agtgcgcaaa gtattgtcca tgccccaagt caatattgtc 8761 aagaaaacag aagtacagac aggcggattc tccaaggagt caattttacc aaaaagaaat 8821 tcggacaagc ttattgctcg taaaaaagac tgggatccaa aaaaatatgg tggttttgat 8881 agtccaacgg tagcttattc agtcctagtg gttgctaagg tggaaaaagg gaaatcgaag 8941 aagttaaaat ccgttaaaga gttactaggg atcacaatta tggaaagaag ttcctttgaa 9001 aaaaatccga ttgacttttt agaagctaaa ggatataagg aagttaaaaa agacttaatc 9061 attaaactac ctaaatatag tctttttgag ttagaaaacg gtcgtaaacg gatgctggct 9121 agtgccggag aattacaaaa aggaaatgag ctggctctgc caagcaaata tgtgaatttt 9181 ttatatttag ctagtcatta tgaaaagttg aagggtagtc cagaagataa cgaacaaaaa 9241 caattgtttg tggagcagca taagcattat ttagatgaga ttattgagca aatcagtgaa 9301 ttttctaagc gtgttatttt agcagatgcc aatttagata aagttcttag tgcatataac 9361 aaacatagag acaaaccaat acgtgaacaa gcagaaaata ttattcattt atttacgttg 9421 acgaatcttg gagctcccgc tgcttttaaa tattttgata caacaattga tcgtaaacga 9481 tatacgtcta caaaagaagt tttagatgcc actcttatcc atcaatccat cactggtctt 9541 tatgaaacac gcattgattt gagtcagcta ggaggtgaca cgcgtttgga gcaagtgaag 9601 ttctggaggg ataaaaatta ataaaagctt gatcagatcc cagctttttt ttgaagtgcc 9661 tggcagtaag ttgaaaaaat ttgaaataaa cttggactat gttaataaaa aaatacaaat 9721 taacgaacaa aaagatttga attattatat aatttagtta aaagcaattt taaatgaggg 9781 aggtttccat catatggata gaagtggtaa acctgaatta actgctacta gtgttgaaaa 9841 atttttaatt gaaaaatttg atagtgttag tgatttaatg caattaagtg aaggtgaaga 9901 aagtagagct tttagttttg atgttggtgg tagaggttat gttttaagag ttaatagttg 9961 tgctgatggt ttttataaag atagatatgt ttatagacat tttgctagtg ctgctttacc 10021 tattcctgaa gttttagata ttggtgaatt tagtgaaagt ttaacttatt gtattagtag 10081 aagagcacaa ggtgttactt tacaagattt acctgaaact gaattacctg ctgttttaca 10141 acctgttgct gaagctatgg atgctattgc ggcggctgat ctcagtcaaa cttcgggttt 10201 tggtcctttt ggtcctcaag gtattggtca atatactact tggagagatt ttatttgtgc 10261 tattgctgat cctcatgttt atcattggca aactgttatg gatgatactg ttagtgctag 10321 tgttgctcaa gcattagatg aattaatgtt atgggctgaa gattgtcctg aagttagaca 10381 tttagttcat gctgattttg gtagtaataa tgttttaact gataatggta gaattactgc 10441 tgttattgat tggagtgaag ctatgtttgg tgatagtcaa tatgaagttg ctaatatttt 10501 tttttggaga ccttggttag cttgtatgga acaacaaact agatattttg aaagaagaca 10561 tcctgaatta gctggtagtc ctagattaag agcttatatg ttaagaattg gtttagatca 10621 attatatcaa agtttagttg atggtaattt tgatgatgct gcttgggctc aaggtagatg 10681 tgatgctatt gttagaagtg gtgctggtac tgttggtaga actcaaattg ctagaagaag 10741 tgctgctgtt tggactgatg gttgtgttga agttttagct gatagtggta atagaagacc 10801 tagtactaga cctagagcta aagaataatg acgtcgccgg agcggtcgag ttctggaccg 10861 accggctcgg gttctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc 10921 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg 10981 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct 11041 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct 11101 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct 11161 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg 11221 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc 11281 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga 11341 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg 11401 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta 11461 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg 11521 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tgggcttttg 11581 ctggcctttt gctcacatgt tcttt //