LOCUS Exported 8094 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Expresses AtPHYB-S (Arabidopsis thaliana) followed by restriction sites. ACCESSION . VERSION . KEYWORDS pOVC1_AtPHYB-S_X SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8094) AUTHORS Tichy AM, Gerrard EJ, Legrand JMD, Hobbs RM, Janovjak H TITLE Engineering Strategy and Vector Library for the Rapid Generation of Modular Light-Controlled Protein-Protein Interactions. JOURNAL J Mol Biol. 2019 May 29. pii: S0022-2836(19)30317-1. doi: 10.1016/j.jmb.2019.05.033. PUBMED 31150735 REFERENCE 2 (bases 1 to 8094) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8094 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 190..569 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 570..773 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 724..744 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 818..837 /label=T7 /note="T7 promoter, forward primer" promoter 818..836 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(3640..3657) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 3646..3870 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 3916..4344 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(4003..4022) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 4213..4234 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(4353..4373) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 4358..4687 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 4538..4673 /label=SV40 ori /note="SV40 origin of replication" primer_bind 4600..4619 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 4754..5548 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(4808..4827) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 5418..5437 /label=Neo-F /note="Neomycin resistance gene, forward primer" polyA_signal 5722..5843 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(5759..5778) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 5813..5832 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(5892..5908) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(5892..5908) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(5905..5927) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 5916..5932 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(5940..5970) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 5985..6006 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(6123..6140) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(6294..6882) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(6374..6393) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(7053..7913) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 7676..7695 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(7914..8018) /gene="bla" /label=AmpR promoter primer_bind complement(8093..18) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" ORIGIN 1 caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg 61 tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt 121 gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat 181 atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag 241 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct 301 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc 361 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg 421 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat 481 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 541 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 601 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 661 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 721 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc 781 taactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga 841 cccaagctga ccgccaccgg tatggtttcc ggagtcgggg gtagtggcgg tggccgtggc 901 ggtggccgtg gcggagaaga agaaccgtcg tcaagtcaca ctcctaataa ccgaagagga 961 ggagaacaag ctcaatcgtc gggaacgaaa tctctcagac caagaagcaa cactgaatca 1021 atgagcaaag caattcaaca gtacaccgtc gacgcaagac tccacgccgt tttcgaacaa 1081 tccggcgaat cagggaaatc attcgactac tcacaatcac tcaaaacgac gacgtacggt 1141 tcctctgtac ctgagcaaca gatcacagct tatctctctc gaatccagcg aggtggttac 1201 attcagcctt tcggatgtat gatcgccgtc gatgaatcca gtttccggat catcggttac 1261 agtgaaaacg ccagagaaat gttagggatt atgcctcaat ctgttcctac tcttgagaaa 1321 cctgagattc tagctatggg aactgatgtg agatctttgt tcacttcttc gagctcgatt 1381 ctactcgagc gtgctttcgt tgctcgagag attaccttgt taaatccggt ttggattcat 1441 tccaagaata ctggtaaacc gttttacgcc attcttcata ggattgatgt tggtgttgtt 1501 attgatttag agccagctag aactgaagat cctgcgcttt ctattgctgg tgctgttcaa 1561 tcgcagaaac tcgcggttcg tgcgatttct cagttacagg ctcttcctgg tggagatatt 1621 aagcttttgt gtgacactgt cgtggaaagt gtgagggact tgactggtta tgatcgtgtt 1681 atggtttata agtttcatga agatgagcat ggagaagttg tagctgagag taaacgagac 1741 gatttagagc cttatattgg actgcattat cctgctactg atattcctca agcgtcaagg 1801 ttcttgttta agcagaaccg tgtccgaatg atagtagatt gcaatgccac acctgttctt 1861 gtggtccagg acgataggct aactcagtct atgtgcttgg ttggttctac tcttagggct 1921 cctcatggtt gtcactctca gtatatggct aacatgggat ctattgcgtc tttagcaatg 1981 gcggttataa tcaatggaaa tgaagatgat gggagcaatg tagctagtgg aagaagctcg 2041 atgaggcttt ggggtttggt tgtttgccat cacacttctt ctcgctgcat accgtttccg 2101 ctaaggtatg cttgtgagtt tttgatgcag gctttcggtt tacagttaaa catggaattg 2161 cagttagctt tgcaaatgtc agagaaacgc gttttgagaa cgcagacact gttatgtgat 2221 atgcttctgc gtgactcgcc tgctggaatt gttacacaga gtcccagtat catggactta 2281 gtgaaatgtg acggtgcagc atttctttac cacgggaagt attacccgtt gggtgttgct 2341 cctagtgaag ttcagataaa agatgttgtg gagtggttgc ttgcgaatca tgcggattca 2401 accggattaa gcactgatag tttaggcgat gcggggtatc ccggtgcagc tgcgttaggg 2461 gatgctgtgt gcggtatggc agttgcatat atcacaaaaa gagactttct tttttggttt 2521 cgatctcaca ctgcgaaaga aatcaaatgg ggaggcgcta agcatcatcc ggaggataaa 2581 gatgatgggc aacgaatgca tcctcgttcg tcctttcagg cttttcttga agttgttaag 2641 agccggagtc agccatggga aactgcggaa atggatgcga ttcactcgct ccagcttatt 2701 ctgagagact cttttaaaga atctgaggcg gctatgaact ctaaagttgt ggatggtgtg 2761 gttcagccat gtagggatat ggcgggggaa caggggattg atgagttagg tgcagttgca 2821 agagagatgg ttaggctcat tgagactgca actgttccta tattcgctgt ggatgccgga 2881 ggctgcatca atggatggaa cgctaagatt gcagagttga caggtctctc agttgaagaa 2941 gctatgggga agtctctggt ttctgattta atatacaaag agaatgaagc aactgtcaat 3001 aagcttcttt ctcgtgcttt gagaggggac gaggaaaaga atgtggaggt taagctgaaa 3061 actttcagcc ccgaactaca agggaaagca gtttttgtgg ttgtgaatgc ttgttccagc 3121 aaggactact tgaacaacat tgtcggcgtt tgttttgttg gacaagacgt tactagtcag 3181 aaaatcgtaa tggataagtt catcaacata caaggagatt acaaggctat tgtacatagc 3241 ccaaaccctc taatcccgcc aatttttgct gctgacgaga acacgtgctg cctggaatgg 3301 aacatggcga tggaaaagct tacgggttgg tctcgcagtg aagtgattgg gaaaatgatt 3361 gtcggggaag tgtttgggag ctgttgcatg ctaaagggtc ctgatgcttt aaccaagttc 3421 atgattgtat tgcataatgc gattggtggc caagatacgg ataagttccc tttcccattc 3481 tttgaccgca atgggaagtt tgttcaggct ctattgactg caaacaagcg ggttagcctc 3541 gagggaaagg ttattggggc tttctgtttc ttgcaaatcc cgagccccgg tgaattctca 3601 ggctctggat cccccgggta agtttaaacc gctgatcagc ctcgactgtg ccttctagtt 3661 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc 3721 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt 3781 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca 3841 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct 3901 ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta 3961 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc 4021 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt 4081 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg 4141 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca 4201 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct 4261 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga 4321 tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa 4381 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac 4441 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa 4501 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag 4561 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc 4621 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt 4681 ttgcaaaaag ctctcgggag cttgtatatc cattttcgga tctgatcaag agacaggatg 4741 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt 4801 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt 4861 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc 4921 cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc 4981 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga 5041 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat 5101 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca 5161 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga 5221 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc 5281 gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat 5341 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga 5401 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg 5461 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt 5521 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa 5581 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg 5641 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg 5701 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc 5761 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg 5821 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg 5881 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac 5941 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc 6001 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg 6061 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct 6121 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 6181 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 6241 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 6301 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 6361 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 6421 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 6481 ctttctcata gctcacgatg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 6541 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 6601 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 6661 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 6721 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 6781 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 6841 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 6901 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 6961 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 7021 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct 7081 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata 7141 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca 7201 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga 7261 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga 7321 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg 7381 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 7441 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt 7501 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 7561 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca 7621 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat 7681 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 7741 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 7801 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 7861 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 7921 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 7981 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 8041 cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc agta //