LOCUS       Exported                2606 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Bacterial Expression plasmid for SARS-CoV-2 nsp6.
ACCESSION   .
VERSION     .
KEYWORDS    pGBW-m4046916
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2606)
  TITLE     SARS-CoV-2 protein E. coli expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    1..25
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             40..62
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             943..963
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             964..981
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     rep_origin      1102..1690
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1591..1610
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1796..2455
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1862..1881)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      2526..2560
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     primer_bind     2588..1
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2588..2606
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggaattgtga gcggataaca attcccctct agaaataatt ttgtttaact ttaagaagga
       61 gactcttcta tgtccgcggt gaagcgtacg attaaaggca ctcaccattg gctgttactt
      121 acaatcctaa ccagcctgct ggtcctcgtt caaagtactc agtggtcgct gttctttttc
      181 ttgtatgaga acgccttcct gccgtttgca atggggatca tagctatgtc agcgttcgct
      241 atgatgtttg ttaaacacaa acatgcattc ttatgtctgt ttctgctgcc aagcctggct
      301 accgtagcat acttcaatat ggtttacatg ccggcttcct gggtgatgcg cattatgact
      361 tggctggata tggtagacac ctctctgtct ggtttcaagc ttaaagactg cgttatgtat
      421 gccagcgcgg tggttctgct gatcctgatg accgctagga ccgtctacga tgatggtgct
      481 cgtcgcgtat ggactctaat gaacgttctg actcttgtat acaaagtcta ttacggcaac
      541 gcactggacc aggcgatctc tatgtgggcc ctgatcattt ctgttacatc caattacagt
      601 ggtgtagtta ccacggtaat gttccttgcc cgtggcattg tgttcatgtg cgtggaatat
      661 tgccctatct tctttatcac tggtaacacc ctgcaatgta tcatgttggt ttactgcttt
      721 ctgggctact tttgcacttg ctatttcggg ctgttctgtt tactcaaccg ttactttcgc
      781 ctgaccctgg gtgtctacga ctacctggtt agcacacagg aatttcgcta catgaacagc
      841 cagggactgc ttcccccgaa aaattctatt gatgctttca aactgaacat caagttgttg
      901 ggcgtgggtg gcaaaccgtg catcaaagtg gcgacggttc aggagaacct gtactttcaa
      961 agccaccatc atcaccatca ttaaccggct tatcggtcag tttcacctga tttacgtaaa
     1021 aacccgcttc ggcgggtttt tgcttttgga ggggcagaaa gatgaatgac tgtccacgac
     1081 gctataccca aaagaaaaag cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     1141 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     1201 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     1261 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     1321 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     1381 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     1441 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     1501 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     1561 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     1621 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     1681 cctatggaaa ttgatcgggc acgtaagagg ttccaacttt caccataatg aaataagatc
     1741 actaccgggc gtattttttg agttatcgag attttcagga gctaaggaag ctaaaatgga
     1801 gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt
     1861 tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac
     1921 ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat
     1981 tcttgcccgc ctgatgaatg ctcatccgga atttcgtatg gcaatgaaag acggtgagct
     2041 ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt
     2101 ttcatcgctc tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca
     2161 agatgtggcg tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat
     2221 gtttttcgtt tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa
     2281 tatggacaac ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa
     2341 ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg
     2401 cagaatgctt aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaatttga
     2461 tatcgagctc gcttggactc ctgttgatag atccagtaat gacctcagaa ctccatctgg
     2521 atttgttcag aacgctcggt tgccgccggg cgttttttat tggtcatcga tctcgatccc
     2581 gcgaaattaa tacgactcac tatagg
//
