LOCUS       Exported               13907 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Lentiviral expression vector for an inducible p53(R280K)-V5.
ACCESSION   .
VERSION     .
KEYWORDS    pSLIK_hygro_p53(R280K)_V5
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13907)
  AUTHORS   Pereira EJ, Burns JS, Lee CY, Marohl T, Calderon D, Wang L, Atkins 
            KA, Wang CC, Janes KA
  TITLE     Sporadic activation of an oxidative stress-dependent NRF2-p53 
            signaling network in breast epithelial spheroids and 
            premalignancies.
  JOURNAL   Sci Signal. 2020 Apr 14;13(627). pii: 13/627/eaba4200. doi: 
            10.1126/scisignal.aba4200.
  PUBMED    32291314
REFERENCE   2  (bases 1 to 13907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13907
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1443..1676
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1861..1905
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2203..2320
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2377..2401
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2433..2747
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2441..2459
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2477..2495
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2512..2530
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2548..2566
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2584..2602
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2619..2637
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2655..2673
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2720..2744
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2754..3932
                     /codon_start=1
                     /gene="Homo sapiens TP53"
                     /product="human tumor suppressor protein that is often 
                     mutated in cancers"
                     /label=p53
                     /note="responds to cellular stresses to regulate the 
                     expression of target genes"
                     /translation="MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLML
                     SPDDIEQWFTEDPGPDEAPRMPEAAPRVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQ
                     GSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIY
                     KQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEP
                     PEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGKD
                     RRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEM
                     FRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD"
     CDS             3966..4007
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    complement(4055..4079)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        4129..5340
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5177..5196
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             5465..6211
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    6352..6814
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6408..6425)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6635..6654
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6873..7892
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     misc_feature    8358..8946
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8411..8431)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8829..8840)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8949..8965)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8950..8966)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9475..9655
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9681..9698)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9687..9911
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9957..10385
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10044..10063)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10254..10275
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10394..10414)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10399..10728
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10579..10714
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10641..10660
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10776..10823
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10842..11216
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    11346..11467
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11383..11402)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11437..11456
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11516..11532)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11516..11532)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11529..11551)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11540..11556
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(11564..11594)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11609..11630
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11747..11764)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11918..12506)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11998..12017)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(12677..13537)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13300..13319
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13538..13642)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(13717..13736)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2161 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2221 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2281 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2341 cagagatcca gtttggttaa ttctagacag ttatcaacaa gtttgtacaa aaaagcaggc
     2401 tttaaaggaa ccaattcagt cgactggatc ccgagtttac tccctatcag tgatagagaa
     2461 cgtatgtcga gtttactccc tatcagtgat agagaacgat gtcgagttta ctccctatca
     2521 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgagtt
     2581 tactccctat cagtgataga gaacgtatgt cgagtttatc cctatcagtg atagagaacg
     2641 tatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgaggtagg cgtgtacggt
     2701 gggaggccta tataagcaga gctcgtttag tgaaccgtca gatcgccact agtatggagg
     2761 agccgcagtc agatcctagc gtcgagcccc ctctgagtca ggaaacattt tcagacctat
     2821 ggaaactact tcctgaaaac aacgttctgt cccccttgcc gtcccaagca atggatgatt
     2881 tgatgctgtc cccggacgat attgaacaat ggttcactga agacccaggt ccagatgaag
     2941 ctcccagaat gccagaggct gctccccgcg tggcccctgc accagcagct cctacaccgg
     3001 cggcccctgc accagccccc tcctggcccc tgtcatcttc tgtcccttcc cagaaaacct
     3061 accagggcag ctacggtttc cgtctgggct tcttgcattc tgggacagcc aagtctgtga
     3121 cttgcacgta ctcccctgcc ctcaacaaga tgttttgcca actggccaag acctgccctg
     3181 tgcagctgtg ggttgattcc acacccccgc ccggcacccg cgtccgcgcc atggccatct
     3241 acaagcagtc acagcacatg acggaggttg tgaggcgctg cccccaccat gagcgctgct
     3301 cagatagcga tggtctggcc cctcctcagc atcttatccg agtggaagga aatttgcgtg
     3361 tggagtattt ggatgacaga aacacttttc gacatagtgt ggtggtgccc tatgagccgc
     3421 ctgaggttgg ctctgactgt accaccatcc actacaacta catgtgtaac agttcctgca
     3481 tgggcggcat gaaccggagg cccatcctca ccatcatcac actggaagac tccagtggta
     3541 atctactggg acggaacagc tttgaggtgc gtgtttgtgc ctgtcctggg aaagaccggc
     3601 gcacagagga agagaatctc cgcaagaaag gggagcctca ccacgagctg cccccaggga
     3661 gcactaagcg agcactgccc aacaacacca gctcctctcc ccagccaaag aagaaaccac
     3721 tggatggaga atatttcacc cttcagatcc gtgggcgtga gcgcttcgag atgttccgag
     3781 agctgaatga ggccttggaa ctcaaggatg cccaggctgg gaaggagcca ggggggagca
     3841 gggctcactc cagccacctg aagtccaaaa agggtcagtc tacctcccgc cataaaaaac
     3901 tcatgttcaa gacagaaggg cctgactcag acaagggctc gagtctagag ggcccgcggt
     3961 tcgaaggtaa gcctatccct aaccctctcc tcggtctcga ttctacgcgt accggttagc
     4021 aattggaaga ctaatgcgtt ttcgagatat ctagacccag ctttcttgta caaagtggtt
     4081 gataacatct cgagccataa agatggttaa ttaacccacc caagatctgg cctccgcgcc
     4141 gggttttggc gcctcccgcg ggcgcccccc tcctcacggc gagcgctgcc acgtcagacg
     4201 aagggcgcag cgagcgtcct gatccttccg cccggacgct caggacagcg gcccgctgct
     4261 cataagactc ggccttagaa ccccagtatc agcagaagga cattttagga cgggacttgg
     4321 gtgactctag ggcactggtt ttctttccag agagcggaac aggcgaggaa aagtagtccc
     4381 ttctcggcga ttctgcggag ggatctccgt ggggcggtga acgccgatga ttatataagg
     4441 acgcgccggg tgtggcacag ctagttccgt cgcagccggg atttgggtcg cggttcttgt
     4501 ttgtggatcg ctgtgatcgt cacttggtga gtagcgggct gctgggctgg ccggggcttt
     4561 cgtggccgcc gggccgctcg gtgggacgga agcgtgtgga gagaccgcca agggctgtag
     4621 tctgggtccg cgagcaaggt tgccctgaac tgggggttgg ggggagcgca gcaaaatggc
     4681 ggctgttccc gagtcttgaa tggaagacgc ttgtgaggcg ggctgtgagg tcgttgaaac
     4741 aaggtggggg gcatggtggg cggcaagaac ccaaggtctt gaggccttcg ctaatgcggg
     4801 aaagctctta ttcgggtgag atgggctggg gcaccatctg gggaccctga cgtgaagttt
     4861 gtcactgact ggagaactcg gtttgtcgtc tgttgcgggg gcggcagtta tggcggtgcc
     4921 gttgggcagt gcacccgtac ctttgggagc gcgcgccctc gtcgtgtcgt gacgtcaccc
     4981 gttctgttgg cttataatgc agggtggggc cacctgccgg taggtgtgcg gtaggctttt
     5041 ctccgtcgca ggacgcaggg ttcgggccta gggtaggctc tcctgaatcg acaggcgccg
     5101 gacctctggt gaggggaggg ataagtgagg cgtcagtttc tttggtcggt tttatgtacc
     5161 tatcttctta agtagctgaa gctccggttt tgaactatgc gctcggggtt ggcgagtgtg
     5221 ttttgtgaag ttttttaggc accttttgaa atgtaatcat ttgggtcaat atgtaatttt
     5281 cagtgttaga ctagtaaatt gtccgctaaa ttctggccgt ttttggcttt tttgttagac
     5341 gaagcttggt accgagctcg gatctccacc ccgtaccggt cctgcaggat ttaaatggcg
     5401 cgccgctagc gctaccggac tcagatctcg agctcaagct tcgaattctg cagtcgaatt
     5461 caccatgtct agactggaca agagcaaagt cataaacgga gctctggaat tactcaatgg
     5521 tgtcggtatc gaaggcctga cgacaaggaa actcgctcaa aagctgggag ttgagcagcc
     5581 taccctgtac tggcacgtga agaacaagcg ggccctgctc gatgccctgc caatcgagat
     5641 gctggacagg catcataccc acttctgccc cctggaaggc gagtcatggc aagactttct
     5701 gcggaacaac gccaagtcat accgctgtgc tctcctctca catcgcgacg gggctaaagt
     5761 gcatctcggc acccgcccaa cagagaaaca gtacgaaacc ctggaaaatc agctcgcgtt
     5821 cctgtgtcag caaggcttct ccctggagaa cgcactgtac gctctgtccg ccgtgggcca
     5881 ctttacactg ggctgcgtat tggaggaaca ggagcatcaa gtagcaaaag aggaaagaga
     5941 gacacctacc accgattcta tgcccccact tctgagacaa gcaattgagc tgttcgaccg
     6001 gcagggagcc gaacctgcct tccttttcgg cctggaacta atcatatgtg gcctggagaa
     6061 acagctaaag tgcgaaagcg gcgggccgac cgacgccctt gacgattttg acttagacat
     6121 gctcccagcc gatgcccttg acgactttga ccttgatatg ctgcctgctg acgctcttga
     6181 cgattttgac cttgacatgc tccccgggta actaagtaag gatccgcggc cgcactagag
     6241 gaattccgcc cctcyccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg
     6301 tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg
     6361 gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg
     6421 aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca
     6481 aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct
     6541 ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca
     6601 cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa
     6661 ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg
     6721 cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg
     6781 ggacgtggtt ttcctttgaa aaacacgatg ataagcttac cggtacgcgt gatagcttgc
     6841 cacaacccgt accaaagatg gatagatccg gaaagcctga actcaccgcg acgtctgtcg
     6901 agaagtttct gatcgaaaag ttcgacagcg tctccgacct gatgcagctc tcggagggcg
     6961 aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata
     7021 gctgcgccga tggtttctac aaagatcgtt atgtttatcg gcactttgca tcggccgcgc
     7081 tcccgattcc ggaagtgctt gacattgggg aattcagcga gagcctgacc tattgcatct
     7141 cccgccgtgc acagggtgtc acgttgcaag acctgcctga aaccgaactg cccgctgttc
     7201 tgcagccggt cgcggaggcc atggatgcga tcgctgcggc cgatcttagc cagacgagcg
     7261 ggttcggccc attcggaccg caaggaatcg gtcaatacac tacatggcgt gatttcatat
     7321 gcgcgattgc tgatccccat gtgtatcact ggcaaactgt gatggacgac accgtcagtg
     7381 cgtccgtcgc gcaggctctc gatgagctga tgctttgggc cgaggactgc cccgaagtcc
     7441 ggcacctcgt gcacgcggat ttcggctcca acaatgtcct gacggacaat ggccgcataa
     7501 cagcggtcat tgactggagc gaggcgatgt tcggggattc ccaatacgag gtcgccaaca
     7561 tcttcttctg gaggccgtgg ttggcttgta tggagcagca gacgcgctac ttcgagcgga
     7621 ggcatccgga gcttgcagga tcgccgcggc tccgggcgta tatgctccgc attggtcttg
     7681 accaactcta tcagagcttg gttgacggca atttcgatga tgcagcttgg gcgcagggtc
     7741 gatgcgacgc aatcgtccga tccggagccg ggactgtcgg gcgtacacaa atcgcccgca
     7801 gaagcgcggc cgtctggacc gatggctgtg tagaagtact cgccgatagt ggaaaccgac
     7861 gccccagcac tcgtccgagg gcaaaggaat agagtagatg ccgaccgaac aagagctgat
     7921 ttcgagaacg cctcagccag caactcgcgc gagcctagca aggcaaatgc gagagaacgg
     7981 ccttacgctt ggtggcacag ttctcgtcca cagttcgcta agctcgctcg gctgggtcgc
     8041 gggagggccg gtcgcagtga ttcaggccct tctggattgt gttggtcccc agggcacgat
     8101 tgtcatgccc acgcactcgg gtgatctgac tgatcccgca gattggagat cgccgcccgt
     8161 gcctgccgat tgggtgcaga tctctagatc tgtacaagta aagcggccgc gactctagat
     8221 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
     8281 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gtttagtccc tcccaattcg
     8341 atatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     8401 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     8461 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     8521 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     8581 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     8641 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     8701 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
     8761 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     8821 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     8881 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     8941 ccccgcatcg ataccgtcga cctcgatcga gacctagaaa aacatggagc aatcacaagt
     9001 agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag
     9061 gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta
     9121 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     9181 agacaagata tccttgatct gtggatctac cacacacaag gctacttccc tgattggcag
     9241 aactacacac cagggccagg gatcagatat ccactgacct ttggatggtg ctacaagcta
     9301 gtaccagttg agcaagagaa ggtagaagaa gccaatgaag gagagaacac ccgcttgtta
     9361 caccctgtga gcctgcatgg gatggatgac ccggagagag aagtattaga gtggaggttt
     9421 gacagccgcc tagcatttca tcacatggcc cgagagctgc atccggactg tactgggtct
     9481 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     9541 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     9601 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagggcc
     9661 cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     9721 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     9781 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     9841 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
     9901 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc
     9961 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
    10021 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
    10081 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
    10141 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
    10201 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
    10261 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
    10321 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
    10381 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
    10441 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
    10501 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
    10561 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
    10621 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta
    10681 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga
    10741 gcttgtatat ccattttcgg atctgatcag cacgtgttga caattaatca tcggcatagt
    10801 atatcggcat agtataatac gacaaggtga ggaactaaac catggccaag ttgaccagtg
    10861 ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg accgaccggc
    10921 tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg gacgacgtga
    10981 ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg gcctgggtgt
    11041 gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc
    11101 gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg cgggagttcg
    11161 ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag gactgacacg
    11221 tgctacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
    11281 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
    11341 accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
    11401 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
    11461 tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat
    11521 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
    11581 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
    11641 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
    11701 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
    11761 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
    11821 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
    11881 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
    11941 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
    12001 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
    12061 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
    12121 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
    12181 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
    12241 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
    12301 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
    12361 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
    12421 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
    12481 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
    12541 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
    12601 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
    12661 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
    12721 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
    12781 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
    12841 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
    12901 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
    12961 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
    13021 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
    13081 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
    13141 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
    13201 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
    13261 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
    13321 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
    13381 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
    13441 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
    13501 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
    13561 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
    13621 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat
    13681 cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg atgccgcata
    13741 gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa
    13801 aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt
    13861 taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgtt
//
